Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,427 C→A 19.1% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4270CA19.1% 1842.0 / 150.0 748Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (207/366);  new base A (156/12);  total (370/378)
Fisher's exact test for biased strand distribution p-value = 6.28e-43
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 762 total aligned reads displayed.

TGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470279‑2470576
                                                                                                                                                    |                                                                                                                                                     
tGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCTGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCa                                                                                                                                                      <  2:1582042/150‑1 (MQ=255)
tGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCa                                                                                                                                                      <  1:4683623/150‑1 (MQ=255)
 gtagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaa                                                                                                                                                     <  2:3395827/150‑1 (MQ=255)
 gtagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaa                                                                                                                                                     <  2:4002760/150‑1 (MQ=255)
  tagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaaa                                                                                                                                                    <  1:174067/150‑1 (MQ=255)
       tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                                                                                               <  2:8319914/150‑1 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                             <  1:6010599/150‑1 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAtaa                                                                                                                                             >  2:5613983/1‑148 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGaaaaaa                                                                                                                                             >  1:7524499/1‑147 (MQ=255)
         tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAATAAAAGAAAACa                                                                                                                                             >  1:10086343/1‑150 (MQ=255)
          ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                            <  2:9217975/150‑1 (MQ=255)
           tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                                                                                                           <  1:10449387/150‑1 (MQ=255)
                ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACaaa                                                                                                                                      >  2:5736548/1‑149 (MQ=255)
                 gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAATAAAAAAAATCAAGaaaaaa                                                                                                                                     >  1:8513895/1‑148 (MQ=255)
                    tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAATACAATATCg                                                                                                                                  >  2:10031307/1‑150 (MQ=255)
                    tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                  <  1:8986007/150‑1 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAATAGCGaatt                                                                                                                              >  1:619060/1‑147 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAAAAATAAAATATCGATGt                                                                                                                              >  2:11625429/1‑150 (MQ=255)
                        tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATAAAGACAAAAGAGAtct                                                                                                                              >  1:7794411/1‑148 (MQ=255)
                          aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                                                                                                            <  2:6618100/150‑1 (MQ=255)
                          aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaa                                                                                                                            <  1:8852838/150‑1 (MQ=255)
                          aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAACGCTGTaa                                                                                                                            >  1:8191028/1‑150 (MQ=255)
                          aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAAAAAAAAAAGATGTaa                                                                                                                            >  1:11024535/1‑150 (MQ=255)
                            ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                          <  1:6369542/150‑1 (MQ=255)
                            ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                          <  1:1993094/150‑1 (MQ=255)
                              ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                        <  2:2750336/150‑1 (MQ=255)
                              ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAACCGATGTAAAGCa                                                                                                                        >  2:10917362/1‑150 (MQ=255)
                                  gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                    <  2:3564560/150‑1 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                 >  2:7121849/1‑150 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAAGAAAAAAACGATTTAAAGCACTAAGtt                                                                                                                 >  2:4409470/1‑150 (MQ=255)
                                     tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAAGAAATAAAGAAAATATCGATGTAAAGCACTAAGtt                                                                                                                 >  2:3746856/1‑150 (MQ=255)
                                      tcattttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                >  2:10739519/4‑150 (MQ=255)
                                       aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGATGTAAAGCAAAAAGTTTa                                                                                                               >  1:375113/1‑150 (MQ=255)
                                       aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAAGACAATAACGATTTAAAGAACGAAGTTTa                                                                                                               >  1:4012263/1‑150 (MQ=255)
                                        tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTATTTTTTTAAATAATTTAAATAAATAAAGACAATATGGGTGTTATGGAGTAAGttttt                                                                                                              >  1:6612248/1‑148 (MQ=255)
                                         ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                             <  1:5181680/150‑1 (MQ=255)
                                         ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTGTTCGTTCGACCTTGGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                             <  2:3854906/150‑1 (MQ=255)
                                                   aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                                                                                                   <  1:286289/150‑1 (MQ=255)
                                                    ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATAAAGAAAAAATCGATTTAAATCACAAAGTTTAAATTGAGCGCCaa                                                                                                  >  2:1656438/1‑150 (MQ=255)
                                                         taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                             <  1:11946733/150‑1 (MQ=255)
                                                          agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTGAAATAAAAAAAGAAAATATCGAAGTGAAGGACGAAGTTTTATTTTCGCGCCAAACATgg                                                                                            >  1:11468336/1‑150 (MQ=255)
                                                           gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAAAAGCCATTTAAATCACTAAGTTTAAATTTCGCGCCAAAAATGgc                                                                                           >  2:4733255/1‑150 (MQ=255)
                                                           gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATCAAAGAAAAAAAGAAAAAATCGATGTAAAGCACTAATTTTAAATTTCGCGCCAAtaaatgc                                                                                           >  2:1204707/1‑144 (MQ=255)
                                                             gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATATCGATGTAAAGCACTAATTTTAAATTTCGCGCCAATCATTgcgc                                                                                         >  2:9702414/1‑150 (MQ=255)
                                                              aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAACAAGACAAAACCGCTGGAAAGCACTAACTTTAAAATTCGCGCCCCGCAAGgcgcg                                                                                        >  1:691757/1‑150 (MQ=255)
                                                                 tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATTGCGCGcac                                                                                     >  2:6966363/1‑150 (MQ=255)
                                                                  aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTTTAAAATAATTAAAAAAAAAAAAAACAATATCGATGTAAATGACAAAGTTTTAATTGAGGGCCCAGCCGGgcgcgcccc                                                                                    >  1:8653930/1‑147 (MQ=255)
                                                                     caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAAAAAAAAAAAAAAAAAAAGATGTAAAAAAAAAATATTAAAATTAGCGCCACACAAGGCGCGCCCaaac                                                                                 >  1:5395653/1‑148 (MQ=255)
                                                                     caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAATAAAAAAAAAAAAAAAAGAATTAAAACAAAAAGATTAAAATTCACGCCCAGCAGGGCGCGCCCaaca                                                                                 >  1:9500349/1‑148 (MQ=255)
                                                                        gcaTTTATTTGGAATGTATTTTTCGTTCGACCTGTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtat                                                                              <  2:6119302/150‑1 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                            <  2:5153662/150‑1 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                            <  2:2982447/150‑1 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                            <  2:734019/150‑1 (MQ=255)
                                                                          atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATTAAATAATTAAAAAAAAAAAAAAAAAAATCGTTTTAAAAAAATAACTTTAAATTTAGAGCCAATCAAAGGGCGGACaaccgaata                                                                            >  1:12196340/1‑143 (MQ=255)
                                                                              atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                        <  2:4827583/150‑1 (MQ=255)
                                                                                 tgcaccgTATTGTTCGTTCGACCTTTCCTTATACTAAACCCTCTTTTTTTTAATTTATAAAATAATTCAAAGAAACCAAGACAATAGCGAGGAAAAGCACTAAGTTTAAATTGCGCGCCTATCATGGCGCGCACAAGCTATAATACCAAc                                                                     <  1:7272594/144‑1 (MQ=255)
                                                                                   gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAcca                                                                   >  2:8072704/1‑149 (MQ=255)
                                                                                   gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATATCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAACaaaca                                                                   >  2:1378861/1‑147 (MQ=255)
                                                                                   gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGAGGTAAAGCACTAAGTTTAAATTGCGCGCCAATAATGGCGCGCACAACCTATAAaaccaacca                                                                   >  2:10161263/1‑149 (MQ=255)
                                                                                    aaTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTGAAAGAAATAAATACAAAAACGATGTAAATCAATAAGTTTAAATTTAGCGCCAAAAAATGCGCGCACAAGCTATAATAACaaacaa                                                                  >  2:10429750/1‑146 (MQ=255)
                                                                                      tttATTTTTCGTTCGACTTTTTTTTATAAATAACAGTCTTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAGACAATAGCGATGTAAACAAATATTTTTAAATTGCGCCCCAATCCTGGCGCGCACAAGCTATAATACCAACCTAAt                                                                <  1:4156041/148‑1 (MQ=255)
                                                                                       gTATTTTTCGTTCGACCTTTGCTTATACATAAATGTCTTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGTTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAAtt                                                               <  1:8389491/150‑1 (MQ=255)
                                                                                         aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                             <  2:11229066/150‑1 (MQ=255)
                                                                                           ttttCGTTCTACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                           <  1:10221547/150‑1 (MQ=255)
                                                                                           ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATAAAATAATTCAAAGAAATCAAGACATTAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                           <  1:10007495/150‑1 (MQ=255)
                                                                                           ttttCGTTCGACCTTTGATTATAAATAACTGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATCGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                           <  1:152099/150‑1 (MQ=255)
                                                                                                gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGCTGTAAAGCACTAAGTTTAAAATGAGCGCCAATCAAGGCGCGCACAAGCGATAATACCAACCAACTTTcacctcca                                                      >  2:8701521/1‑149 (MQ=255)
                                                                                                 ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAATAATTAAAAGAAAAAAAGAAAATATCGATGTAAATAACTAAGTTTAAATTGAGCGCCAATAATGGCGCGCACAAGCAATAATAACAAACTAAATtctaatacac                                                     >  2:6248180/1‑143 (MQ=255)
                                                                                                    gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                  <  1:4843070/150‑1 (MQ=255)
                                                                                                      ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAAAAAAGCGCTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAACAACCAAATTTCTCCTCCTCTTaga                                                >  2:7498417/1‑150 (MQ=255)
                                                                                                        tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCGATAATACCAACCTAATTTCTCCACCACGTagagg                                              >  2:7844160/1‑149 (MQ=255)
                                                                                                        tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAGCGATTTAAAGCACAAAGTTTAAATTTCGCGCCAATAATGGCGCGCACAAGCTATAATACCAACCTAATTTAACCTCCTCTTagatt                                              >  2:1841113/1‑148 (MQ=255)
                                                                                                          tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                            <  2:8994167/150‑1 (MQ=255)
                                                                                                           gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                           <  1:9213906/150‑1 (MQ=255)
                                                                                                           gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                           <  2:3134043/150‑1 (MQ=255)
                                                                                                            cTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATCAAAGAAATCAAGACAATATCGAAGTAAAGCACTAAGTTTAAATTTCGCGCCAAACAAGGCGCGCACAAGCGATAATACCAACCAAATTTAACCTCCGCATAGAGTGACt                                          >  2:9470074/1‑150 (MQ=255)
                                                                                                                   aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACAAAAGCGATGTAAAGCACGAAGTTTAAATTGCGCGCCAAACCTGGCGCGCACAAGCTATAATACAAAACTAATTTATCCTCCACATAGAGTTACTAAAtcac                                   >  2:12175470/1‑148 (MQ=255)
                                                                                                                   aTAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAACAAGACAAAAGCCAAGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAACCAATAAAACCAACCAAATTTCTCCTCCTCATAGAGTGACtataaatc                                   >  2:12234032/1‑146 (MQ=255)
                                                                                                                         gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                             <  2:7659403/150‑1 (MQ=255)
                                                                                                                            gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATCGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                          <  2:2471578/150‑1 (MQ=255)
                                                                                                                             tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                         >  1:11695482/1‑150 (MQ=255)
                                                                                                                             tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                         >  1:10308307/1‑150 (MQ=255)
                                                                                                                              ttttttAATTTATAAAATAATTCAAAGAAATCACGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                        <  2:11079717/150‑1 (MQ=255)
                                                                                                                              ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTCAAGCACTAAGTTTAAATTGCGCTCCAATCATGTCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                        <  2:4981547/150‑1 (MQ=255)
                                                                                                                               tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                       >  1:5939283/1‑150 (MQ=255)
                                                                                                                               tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                       <  1:7937550/150‑1 (MQ=255)
                                                                                                                                 tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                     <  2:2071277/150‑1 (MQ=255)
                                                                                                                                  ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAGTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTGAGAGTGACTATATCTCCTGATAGAATTGCgg                    <  1:9771665/150‑1 (MQ=255)
                                                                                                                                      tttATAAAATAATTCAAATAATTCAATCCAATAGCGCTTTAAAGCACTATGTTTATTTTGCGCGCCAATCCTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTCGAGTGACTATCTCTCCTGATAGAATTGCGGTAtt                <  1:3304005/150‑1 (MQ=255)
                                                                                                                                      tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                <  1:11220030/150‑1 (MQ=255)
                                                                                                                                       ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg               >  1:2296309/1‑150 (MQ=255)
                                                                                                                                        tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa              <  2:5980286/150‑1 (MQ=255)
                                                                                                                                          taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt            >  1:3478161/1‑150 (MQ=255)
                                                                                                                                            aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt          <  1:551435/150‑1 (MQ=255)
                                                                                                                                                aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa      <  1:10802297/150‑1 (MQ=255)
                                                                                                                                                  ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAACTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg    <  2:4160231/150‑1 (MQ=255)
                                                                                                                                                  ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg    <  2:11860541/150‑1 (MQ=255)
                                                                                                                                                   tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   >  2:220276/1‑150 (MQ=255)
                                                                                                                                                   tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   <  1:2470814/150‑1 (MQ=255)
                                                                                                                                                   tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   >  2:5338345/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470279‑2470576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: