Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,436 C→A 16.4% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4360CA16.4% 2086.3 / 123.1 763Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (239/367);  new base A (147/9);  total (387/376)
Fisher's exact test for biased strand distribution p-value = 2.50e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 776 total aligned reads displayed.

CTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470287‑2470585
                                                                                                                                                     |                                                                                                                                                     
 tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCa                                                                                                                                                      >  2:12233122/1‑150 (MQ=255)
 tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                                      <  1:8356867/150‑1 (MQ=255)
  ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                     <  2:2779844/150‑1 (MQ=255)
   tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                                                                                                                    <  2:5355713/150‑1 (MQ=255)
    ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGATTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                                   <  2:1669409/150‑1 (MQ=255)
    ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAAGAAAACaaaa                                                                                                                                                   >  2:1080025/1‑148 (MQ=255)
      ttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACa                                                                                                                                                 >  2:4991155/1‑150 (MQ=255)
         gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAATAAATCAAGaaaaaa                                                                                                                                              >  2:7347468/1‑148 (MQ=255)
            taactcAATTTTCTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                           <  1:8053315/147‑1 (MQ=255)
              tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTAGACCCTTGCTTATACCTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  2:4828169/150‑1 (MQ=255)
                tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAGAAAAAAGCGATGt                                                                                                                                       >  2:10354250/1‑150 (MQ=255)
                tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGACAAAAGCGATGt                                                                                                                                       >  2:7826005/1‑150 (MQ=255)
                tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAttt                                                                                                                                       >  1:5988126/1‑148 (MQ=255)
                  aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAAAAAAAAAAGATGTaa                                                                                                                                     >  1:11024535/1‑150 (MQ=255)
                   aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                                    <  1:1011391/150‑1 (MQ=255)
                   aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                                    <  2:7622403/150‑1 (MQ=255)
                             tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGtt                                                                                                                          >  2:6656462/1‑150 (MQ=255)
                             tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGCTGTAAAGCACTAAGtt                                                                                                                          >  2:3464373/1‑150 (MQ=255)
                             tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAACAAAATAACGAAGTAAAGCaaaaactt                                                                                                                          >  2:524839/1‑143 (MQ=255)
                              cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTATCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                         <  2:1960307/150‑1 (MQ=255)
                                tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                       <  1:8720371/150‑1 (MQ=255)
                                tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                       <  2:11512659/150‑1 (MQ=255)
                                tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTATTTTTTTAAATAATTTAAATAAATAAAGACAATATGGGTGTTATGGAGTAAGttttt                                                                                                                       >  1:6612248/1‑148 (MQ=255)
                                 ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  1:9972076/150‑1 (MQ=255)
                                 ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  1:6252683/150‑1 (MQ=255)
                                 ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTGTTCGTTCGACCTTGGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  2:3854906/150‑1 (MQ=255)
                                   ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                                    <  2:10147481/150‑1 (MQ=255)
                                    tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                                                                                                                   <  1:5182108/150‑1 (MQ=255)
                                     cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATAAATACAAAAGCGATGTAAAGCACAAAGTTTaaaattc                                                                                                                  >  1:9660278/1‑146 (MQ=255)
                                      ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAATTAAAAAAAAAAAAGAAAAAATAGATGTAAATCACAAAGTTTAAAtttag                                                                                                                 >  1:4101379/1‑147 (MQ=255)
                                           aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                                                                                                            <  1:846837/150‑1 (MQ=255)
                                            ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCATGACAATAGCGATGTAAAGGACTAAGTTTAAATTGCGCGCCag                                                                                                           >  2:7143266/1‑149 (MQ=255)
                                               tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                        <  1:2838214/150‑1 (MQ=255)
                                                 taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                      <  2:8312932/150‑1 (MQ=255)
                                                 taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAAAATCGATGTAAAGCAAAAACTTTAAATTTAGCGCCAATCagg                                                                                                      >  1:10236315/1‑148 (MQ=255)
                                                  agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTGAAATAAAAAAAGAAAATATCGAAGTGAAGGACGAAGTTTTATTTTCGCGCCAAACATgg                                                                                                     >  1:11468336/1‑150 (MQ=255)
                                                  agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAACAAGACAATAGCGATGTAAAGGACTAAGTTTAAATTGGGCGCCAATAATgg                                                                                                     >  2:1318053/1‑150 (MQ=255)
                                                   gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAAAAGCCATTTAAATCACTAAGTTTAAATTTCGCGCCAAAAATGgc                                                                                                    >  2:4733255/1‑150 (MQ=255)
                                                     gaTTTATGCTTGCATTTTTTTGGATTGTATTTTTCGTTCGACCTTTGCTTATACATTTCAGTCGTTTTTTTTATTTATAATATAATTCAATGAAATCAAGACAATAGGGTTGTAAAGCACTATGTTTTAATTGCGCGCCAATCATGgggc                                                                                                  <  1:7893586/150‑1 (MQ=255)
                                                     gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                  <  1:9155721/150‑1 (MQ=255)
                                                     gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCCTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                  <  1:5814020/150‑1 (MQ=255)
                                                        ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                               <  2:12162298/150‑1 (MQ=255)
                                                         tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATTGCGCGcac                                                                                              >  2:6966423/1‑150 (MQ=255)
                                                            gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                           <  2:6372091/150‑1 (MQ=255)
                                                             caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAAGAAAAAAAGAAAAAAGCGAAGTAAATCACAAAGTTTAAAATGAGCGCCCCACAAAGCGCGCACaaaa                                                                                          >  1:6879481/1‑148 (MQ=255)
                                                                 caTTTATTTGGAATGTATTTTTCGTTTGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtata                                                                                      <  2:4528815/150‑1 (MQ=255)
                                                                  atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                                     <  2:10700937/150‑1 (MQ=255)
                                                                  atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATTAAATAATTAAAAAAAAAAAAAAAAAAATCGTTTTAAAAAAATAACTTTAAATTTAGAGCCAATCAAAGGGCGGACaaccgaata                                                                                     >  1:12196340/1‑143 (MQ=255)
                                                                   tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAAAAAAAAAAAAATCGATTTTAATAACAAAGATTAAATTTAGCGCCCAACAAGGCGCGCCCAAGcaaaaaa                                                                                    >  1:1928743/1‑144 (MQ=255)
                                                                           gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAcca                                                                            >  2:8072704/1‑149 (MQ=255)
                                                                               gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                                        <  2:8741124/150‑1 (MQ=255)
                                                                                 aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                                      <  2:375155/150‑1 (MQ=255)
                                                                                 aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                                      <  2:11228857/150‑1 (MQ=255)
                                                                                  tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATAAAAAAGCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCGATAATAACAACCGAAtttat                                                                     >  2:9520726/1‑148 (MQ=255)
                                                                                  tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAAAAAAAAAGCCATTTAAATCACTAATTTTAAATTTCGCCCCAAAAAAGGCGCGCACAAGCTATAATAACAAACAAAtttat                                                                     >  2:1201449/1‑148 (MQ=255)
                                                                                   ttttCGTTCGACCTTTTCTTATACATAACTTTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  1:3900685/150‑1 (MQ=255)
                                                                                   ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATAAAATAATTCAAAGAAATCAAGACATTAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  1:10007495/150‑1 (MQ=255)
                                                                                   ttttCGTACGACCTTTGCTTATACATAACACTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                    <  1:1691508/150‑1 (MQ=255)
                                                                                        gttcgACCTTTGCTTATACATAACCGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATCGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                               <  1:1514867/150‑1 (MQ=255)
                                                                                        gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                               <  1:7134272/150‑1 (MQ=255)
                                                                                        gttcgACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                               <  1:509467/150‑1 (MQ=255)
                                                                                         ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACCATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                              <  2:11069786/150‑1 (MQ=255)
                                                                                              ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                         <  1:10519824/150‑1 (MQ=255)
                                                                                              ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAATTCTCCTCCTCTTaga                                                         >  2:2996765/1‑150 (MQ=255)
                                                                                               cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAAAAAAGACAAAAGCGAAGTAAAGCACGAAGTTTAAAATTCCCGCCAAACAATGCGCGCACAAGCTATTATAACAAACAAATATATACTCCTCTTagag                                                        >  2:9275046/1‑150 (MQ=255)
                                                                                                      tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                                 <  1:10354919/150‑1 (MQ=255)
                                                                                                      tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                                 <  2:2441661/150‑1 (MQ=255)
                                                                                                        taCATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAAAAAAGACAAAAGCCAATTAAAGCACAAAGTTTAAATTTCGCGCCAATCATGGCGCCCACAAGCAAAAATAACAAACAAAATTATACAACACTTAGAGTGACtataa                                               >  2:11765036/1‑149 (MQ=255)
                                                                                                               cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                        <  2:7467985/150‑1 (MQ=255)
                                                                                                                 gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                      <  1:2163710/150‑1 (MQ=255)
                                                                                                                     tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                  >  1:11694754/1‑150 (MQ=255)
                                                                                                                        ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                               <  2:1585509/150‑1 (MQ=255)
                                                                                                                         tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              >  1:11900380/1‑150 (MQ=255)
                                                                                                                         tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  2:2071277/150‑1 (MQ=255)
                                                                                                                         tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  2:4204667/150‑1 (MQ=255)
                                                                                                                         tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  2:9709260/150‑1 (MQ=255)
                                                                                                                          ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCCACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                             <  2:4004310/150‑1 (MQ=255)
                                                                                                                          ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                             >  1:8922492/1‑150 (MQ=255)
                                                                                                                            aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                           <  1:8649589/150‑1 (MQ=255)
                                                                                                                              tttATAAAATAATTCAAATAATTCAATCCAATAGCGCTTTAAAGCACTATGTTTATTTTGCGCGCCAATCCTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTCGAGTGACTATCTCTCCTGATAGAATTGCGGTAtt                         <  1:3304005/150‑1 (MQ=255)
                                                                                                                              tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                         <  1:11220030/150‑1 (MQ=255)
                                                                                                                                tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       >  1:9446469/1‑150 (MQ=255)
                                                                                                                                tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       >  1:10795754/1‑150 (MQ=255)
                                                                                                                                 ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                      <  2:619060/150‑1 (MQ=255)
                                                                                                                                 ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                      <  2:2612866/150‑1 (MQ=255)
                                                                                                                                  taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTCTTGACt                     >  2:9067621/1‑150 (MQ=255)
                                                                                                                                     aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                  >  1:6158334/1‑150 (MQ=255)
                                                                                                                                      ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa                 <  1:4723076/150‑1 (MQ=255)
                                                                                                                                          ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg             <  1:3746856/150‑1 (MQ=255)
                                                                                                                                           tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc            <  1:2470814/150‑1 (MQ=255)
                                                                                                                                           tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc            >  2:3624226/1‑150 (MQ=255)
                                                                                                                                           tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc            >  2:5661368/1‑150 (MQ=255)
                                                                                                                                            cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc           <  2:6097422/150‑1 (MQ=255)
                                                                                                                                             aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATCGACTATCAAATGccc          <  1:5484219/150‑1 (MQ=255)
                                                                                                                                             aaaGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTCTATCTCCTGATAGAATTGCGGTATTGACTCTCAAATGccc          >  1:7375269/1‑150 (MQ=255)
                                                                                                                                              aggAAATCAAGACAATTGCGATGTAAAGCACTAAGTTTAATTTGCGCGCCAATCCTGGCGCGCCCGAGCTATAATACCAACCGAATTTCTCCTCCTCTTAGTGTGACTCTATGTCCTGTTAGAAGGGCGGTCTGGTCTATGATATGccgt         >  2:3168063/3‑148 (MQ=255)
                                                                                                                                              aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         >  1:535907/1‑150 (MQ=255)
                                                                                                                                              aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  1:7783430/150‑1 (MQ=255)
                                                                                                                                              aaGAAATAAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCACTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  1:3464142/150‑1 (MQ=255)
                                                                                                                                                     cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt  <  2:5939188/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470287‑2470585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: