Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,439 G→T 5.4% D437Y (GAC→TAC) ‡ gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4390GT 5.4% 2324.6 / 3.8 764D437Y (GAC→TAC) ‡gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (307/377);  new base T (34/4);  total (383/381)
Fisher's exact test for biased strand distribution p-value = 3.37e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 775 total aligned reads displayed.

TTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGT  >  NC_000913/2470290‑2470588
                                                                                                                                                     |                                                                                                                                                     
tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAg                                                                                                                                                       >  2:4204232/1‑150 (MQ=255)
tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTACAAGAAATAAAg                                                                                                                                                       >  2:2153010/1‑150 (MQ=255)
         tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                              <  2:12143677/150‑1 (MQ=255)
         tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                              <  1:6617426/150‑1 (MQ=255)
            ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAATACAATAGCGATg                                                                                                                                           >  1:10118399/1‑150 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGt                                                                                                                                          >  2:11167960/1‑150 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAATAGCGaatt                                                                                                                                          >  1:619060/1‑147 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                          >  2:8210166/1‑150 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAATCAAGACAAAAGCGATGt                                                                                                                                          >  2:4983042/1‑150 (MQ=255)
             tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAttt                                                                                                                                          >  1:5988126/1‑148 (MQ=255)
                  tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                                                                                                                     <  1:11194593/150‑1 (MQ=255)
                   ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                                    <  1:10245898/150‑1 (MQ=255)
                   ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATAAAAAAGCGATTTAAATCa                                                                                                                                    >  1:7914270/1‑150 (MQ=255)
                         gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAACAAGACAAAAGAGATGTTAATCACGaatt                                                                                                                              >  1:9315027/1‑148 (MQ=255)
                          tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGtt                                                                                                                             >  2:6656462/1‑150 (MQ=255)
                          tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAAGAAAAAAACGATTTAAAGCACTAAGtt                                                                                                                             >  2:4409470/1‑150 (MQ=255)
                              ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                         <  1:8529757/150‑1 (MQ=255)
                              ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                         <  2:9547986/150‑1 (MQ=255)
                              ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAATCGATGTGAAAAACAAAGTGTaaa                                                                                                                         >  1:9035661/1‑150 (MQ=255)
                                 tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTg                                                                                                                      <  1:5182108/150‑1 (MQ=255)
                                  cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATAAATACAAAAGCGATGTAAAGCACAAAGTTTaaaattc                                                                                                                     >  1:9660278/1‑146 (MQ=255)
                                   ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAAGACAAAAGCGATGTAAAGCACGAAGTTTTAATTtcg                                                                                                                    >  2:3739904/1‑150 (MQ=255)
                                      gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATTAAATAATTCAAAGTAATAAAAAAAAAATCGATTTAAAGCACGAAGTTTAAATTtcgcgg                                                                                                                 >  1:11833523/1‑149 (MQ=255)
                                         ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATAAAGAAAAAATCGATTTAAATCACAAAGTTTAAATTGAGCGCCaa                                                                                                              >  2:1656438/1‑150 (MQ=255)
                                           atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAGACAAAAAAGAGGTAAAGCACAAAGATTAAAATTAGCGCCaccc                                                                                                            >  1:3140132/1‑147 (MQ=255)
                                              taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAAATAAAAAAAATAAAGAAAAAAAAGATTTAAATCACTAAGGTTAAAATTAGCGCCCAACATg                                                                                                         >  1:3685876/1‑150 (MQ=255)
                                              taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCTAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                         <  2:7219744/150‑1 (MQ=255)
                                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAAAAAAGAAAAAATCGGTGTAAAGAACTAAGTTTTAATTTCGCGCCAAACATgg                                                                                                        >  2:10307776/1‑150 (MQ=255)
                                                gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                       <  2:3559457/150‑1 (MQ=255)
                                                gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAAAAGCCATTTAAATCACTAAGTTTAAATTTCGCGCCAAAAATGgc                                                                                                       >  2:4733255/1‑150 (MQ=255)
                                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAAAAATACAAAATCGATGTAAAGCACAAAGTTTATATTTAGCGCCACGCATGgcgc                                                                                                     >  1:779325/1‑150 (MQ=255)
                                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCCTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                     <  1:5814020/150‑1 (MQ=255)
                                                   aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAACAAGACAAAACCGCTGGAAAGCACTAACTTTAAAATTCGCGCCCCGCAAGgcgcg                                                                                                    >  1:691757/1‑150 (MQ=255)
                                                     ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                                  <  2:2728111/150‑1 (MQ=255)
                                                      tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATTGCGCGcac                                                                                                 >  2:6966423/1‑150 (MQ=255)
                                                        agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                               <  2:8577825/150‑1 (MQ=255)
                                                         gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                              <  2:6372091/150‑1 (MQ=255)
                                                         gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAAAAAAGACAAAAGAGAATTAAAGAACAAAGTTTAAATTTAGCGCCCATAAAGGCGCGCACAAg                                                                                              >  2:9296714/1‑150 (MQ=255)
                                                          caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATAAAATAGCGAATTAAAGCACTAAGTTTAAATTGAGCGGCAATCATGGCGCGCACAAGc                                                                                             >  2:8770784/1‑150 (MQ=255)
                                                          caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAAAAAATACAAAAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACaaac                                                                                             >  2:10520623/1‑148 (MQ=255)
                                                           aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAATAATTCAAAGAAAAAAAAACAAAAGCGATGTAAAGCACTAACTTTAAAATTCGCGCCAATCAATGCCCGCACAACCt                                                                                            >  2:10799604/1‑150 (MQ=255)
                                                               ttttatttTGATTGTATTTTTGTTTCGACCTTTTCTTATAAATAACAGTGTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                                        <  1:7559724/149‑1 (MQ=255)
                                                                tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGACAAAAGCGATGTAAATCAAAAAGTTTAAATTTAGCGCCAATAATGGCGCGCACAAGCTataat                                                                                       >  2:11477406/1‑150 (MQ=255)
                                                                   atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                                    <  2:4827583/150‑1 (MQ=255)
                                                                      tgcaccgTATTGTTCGTTCGACCTTTCCTTATACTAAACCCTCTTTTTTTTAATTTATAAAATAATTCAAAGAAACCAAGACAATAGCGAGGAAAAGCACTAAGTTTAAATTGCGCGCCTATCATGGCGCGCACAAGCTATAATACCAAc                                                                                 <  1:7272594/144‑1 (MQ=255)
                                                                      tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                                 <  2:3157452/150‑1 (MQ=255)
                                                                        gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAAATAAAAAAAAAAAAGAAAAAAAAGAAGTAAAAAACAAAATTTATATTTAGCGCCAAAAAAGGCGCGCACAACCGATAAAAACaaaca                                                                               >  1:11584483/1‑147 (MQ=255)
                                                                               tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAATCAAGAAAAAATAGATTTAAAGCACAAAGTTTAAAATTCGCGCCAATAAAGGCGCGCACAAGCTATAATACCAACCAAAtttat                                                                        >  2:7361871/1‑148 (MQ=255)
                                                                                ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATAAAATAATTCAAAGAAATCAAGACATTAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                       <  1:10007495/150‑1 (MQ=255)
                                                                                ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                       <  2:2025957/150‑1 (MQ=255)
                                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAAAAAGACAAAATCGATGTAAAGCAATAATTTTAAATTTCGCGCCCATCATGGCGCGCACCAGCGATAAAACCAACAAAATTTctacacct                                                                  >  2:3279844/1‑150 (MQ=255)
                                                                                     gttcgACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                                  <  1:509467/150‑1 (MQ=255)
                                                                                      ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATAATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                                 >  2:10141762/1‑150 (MQ=255)
                                                                                         gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                              <  2:7914165/150‑1 (MQ=255)
                                                                                          aCCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATTAAATAATTCAAAGAAATAAAGAAAATAGCGATGTAAATCACAAATTTTAAATTGCGCGCCAATAATTGCGCGCACAAGCTATAATAACAAACAAATTTATACTCCTCTTag                                                             >  2:9101820/1‑150 (MQ=255)
                                                                                           ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAAAAAAGCGCTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAACAACCAAATTTCTCCTCCTCTTaga                                                            >  2:7498417/1‑150 (MQ=255)
                                                                                             tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAGCGATTTAAAGCACAAAGTTTAAATTTCGCGCCAATAATGGCGCGCACAAGCTATAATACCAACCTAATTTAACCTCCTCTTagatt                                                          >  2:1841113/1‑148 (MQ=255)
                                                                                               tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                        <  1:6415033/150‑1 (MQ=255)
                                                                                               tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                        <  2:12205650/150‑1 (MQ=255)
                                                                                               tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                        <  2:1313868/150‑1 (MQ=255)
                                                                                                     taCATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAAAAAAGACAAAAGCCAATTAAAGCACAAAGTTTAAATTTCGCGCCAATCATGGCGCCCACAAGCAAAAATAACAAACAAAATTATACAACACTTAGAGTGACtataa                                                  >  2:11765036/1‑149 (MQ=255)
                                                                                                        aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAAACATGGCGCGCACAAGCTATAATAACAACCAAATTTATCCTCCTCTTATAGTGACTATAtctc                                               >  2:2991409/1‑150 (MQ=255)
                                                                                                        aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                               <  1:8884902/150‑1 (MQ=255)
                                                                                                        aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAAAATATCGATGTAAAGCAATAAGTTTAAATTGCCCGCCAATCATTGCGCGCACAAGCGATAAAAACAAACTAATTTCTCCACCACTTAGAGTGACTATAtatc                                               >  2:3833042/1‑150 (MQ=255)
                                                                                                            cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                           <  1:4048278/150‑1 (MQ=255)
                                                                                                              gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                         <  2:7659403/150‑1 (MQ=255)
                                                                                                              gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACAATATATCCAGATa                                         >  2:1529559/1‑150 (MQ=255)
                                                                                                               tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCGCTTAGAGTGACTATATCTCCTGATAg                                        >  2:5253420/1‑150 (MQ=255)
                                                                                                                 gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                      >  2:2464167/1‑150 (MQ=255)
                                                                                                                  tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                     >  1:8691653/1‑150 (MQ=255)
                                                                                                                  tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                     >  1:8177465/1‑150 (MQ=255)
                                                                                                                  tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                     >  1:4348669/1‑150 (MQ=255)
                                                                                                                      tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATGGCGCGCACAAGCTCTAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                 >  1:11019979/1‑150 (MQ=255)
                                                                                                                      tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                 <  1:10539516/150‑1 (MQ=255)
                                                                                                                      tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                 <  2:9709260/150‑1 (MQ=255)
                                                                                                                       ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                                <  2:4004195/150‑1 (MQ=255)
                                                                                                                         aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                              >  2:5671311/1‑150 (MQ=255)
                                                                                                                           tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTAtt                            >  1:3769948/1‑150 (MQ=255)
                                                                                                                           tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                            <  2:10968844/150‑1 (MQ=255)
                                                                                                                             tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                          >  1:10795241/1‑150 (MQ=255)
                                                                                                                             tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                          <  1:3395859/150‑1 (MQ=255)
                                                                                                                                   ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa                    <  1:4744739/150‑1 (MQ=255)
                                                                                                                                     aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                  <  1:5508693/150‑1 (MQ=255)
                                                                                                                                     aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                  <  2:6211285/150‑1 (MQ=255)
                                                                                                                                        tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc               <  1:1555606/150‑1 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  2:6097422/150‑1 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAACTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  1:1841128/150‑1 (MQ=255)
                                                                                                                                          aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc             >  1:1363333/1‑150 (MQ=255)
                                                                                                                                          aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc             >  1:1139114/1‑150 (MQ=255)
                                                                                                                                             gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa          <  1:2104568/150‑1 (MQ=255)
                                                                                                                                              aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt         <  2:5405567/150‑1 (MQ=255)
                                                                                                                                                  cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt     <  1:10355112/150‑1 (MQ=255)
                                                                                                                                                  cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt     <  2:5939188/150‑1 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATACTACCAACCTAATTTCTCCTCATCTTAGAGTGACTATATCTCCTGATAGAATTGCGCTATTGACTATCAAATGCCCTGATTCgta    >  1:2322964/1‑149 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    >  1:8394738/1‑150 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    <  1:9888448/150‑1 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    <  1:5385772/150‑1 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    <  2:12014353/150‑1 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    <  1:12161438/150‑1 (MQ=255)
                                                                                                                                                     gACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgt  >  1:272028/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGT  >  NC_000913/2470290‑2470588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: