Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,714,133 A→C 6.8% K413T (AAG→ACG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,714,1330AC 6.8% 1980.5 / 19.6 623K413T (AAG→ACG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (351/213);  new base C (3/55);  total (354/269)
Fisher's exact test for biased strand distribution p-value = 1.81e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 678 total aligned reads displayed.

AGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACT  >  NC_000913/2713984‑2714282
                                                                                                                                                     |                                                                                                                                                     
aGGCCGCTATATTGAAGAGCCAATGAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAagagaaagagaa                                                                                                                                                       <  2:10530187/150‑1 (MQ=255)
  gCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGc                                                                                                                                                     >  2:3260621/1‑150 (MQ=255)
  gCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGCGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGc                                                                                                                                                     >  2:1910535/1‑150 (MQ=255)
    cGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCcg                                                                                                                                                   >  2:8365436/1‑150 (MQ=255)
     gCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCcgc                                                                                                                                                  >  2:2434054/1‑150 (MQ=255)
           ttGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGATAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGTGTGa                                                                                                                                            <  2:9035022/150‑1 (MQ=255)
           ttGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGa                                                                                                                                            <  2:10680792/150‑1 (MQ=255)
            tGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGaa                                                                                                                                           <  2:9321223/150‑1 (MQ=255)
                gagCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGaaaaaa                                                                                                                                       >  2:6281581/1‑150 (MQ=255)
                   ccAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGc                                                                                                                                    >  2:9594886/1‑150 (MQ=255)
                   ccAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGc                                                                                                                                    >  2:6809201/1‑150 (MQ=255)
                   ccAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGc                                                                                                                                    >  2:4198476/1‑150 (MQ=255)
                      aTTAAAGCTCGCGTTATTGATGAGATACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCAt                                                                                                                                 >  1:7296616/1‑150 (MQ=255)
                                gcgTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCa                                                                                                                       >  2:12194278/1‑150 (MQ=255)
                                   ttATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCaaaa                                                                                                                    >  2:11821166/1‑150 (MQ=255)
                                        tatgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAATAAAGTACTGGATAAACGTGCTTATAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               <  1:7990628/149‑1 (MQ=255)
                                        gttgttttACGCCCTAAAAGGGGTGCGCAACGCTAAAAGCTGACGGGAAAGCCATCTATGAATGTACTGGCTAAAGGTGCTGTTAACAAAAAAGCTAAAGATAAAGAGCCGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               <  1:4094945/144‑1 (MQ=255)
                                        gttgaTTTACGCCCTAAAACGCGTGCGCCAAGCTAAAAGCACACGGGCACTCAATCTAATAATGTTGTGGATAAAGGTGCTTATAATAAAAAAGCTAATGAGAAAGACACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               <  1:5754955/148‑1 (MQ=255)
                                        gttgaGTTCCGCCCTAAAACGCGTGCGCCAAGCTAAAAGATGACCGGCAAGCCATCTAATAAAGTACTGGATAAAGGTGCTTAGAATAAAAAAGATAAAGACAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               <  1:1494812/148‑1 (MQ=255)
                                        gatgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               <  2:6409257/150‑1 (MQ=255)
                                         atgtGTTACGCCCTAAAAGGCGTGCGCCACGCGAAAAGCAGACCGGCAAGCCATCTAAGAAAGTACTGGCTAAACGTGCTGAGAATAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTg                                                                                                              <  2:4684042/150‑1 (MQ=255)
                                           gaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAATAAAGTACTGGCTAAACGTGCTGAGCAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGt                                                                                                            <  2:6492929/150‑1 (MQ=255)
                                            aGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTa                                                                                                           >  1:8445143/1‑150 (MQ=255)
                                             gTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTaa                                                                                                          <  2:8608382/150‑1 (MQ=255)
                                                aCGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAgcg                                                                                                       >  1:4313390/1‑150 (MQ=255)
                                                aCGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAgcg                                                                                                       >  2:10418030/1‑150 (MQ=255)
                                                   cccGAAAACGCGTGCGCCAACCTAAAATCAGACCGGAAAGCAATCTAATAATGTACTGGCTAAACGTGCTGAGAATAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCg                                                                                                    <  1:12099373/150‑1 (MQ=255)
                                                    ccGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTCAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAACAATATTGGTAAGCGCCGt                                                                                                   >  1:8783285/1‑150 (MQ=255)
                                                    ccGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGt                                                                                                   >  1:1662468/1‑150 (MQ=255)
                                                    ccGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCAACGCGACACCAAAAATATAGGTAAGCGCCGt                                                                                                   >  1:4842164/1‑150 (MQ=255)
                                                     cggaaaCGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAACGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATCTTGGTCAGCGCCGTa                                                                                                  >  1:8253645/4‑150 (MQ=255)
                                                     cGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTa                                                                                                  >  2:5531602/1‑150 (MQ=255)
                                                     cGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTa                                                                                                  >  2:5116634/1‑150 (MQ=255)
                                                       aaaaCGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTaaa                                                                                                <  2:3792960/150‑1 (MQ=255)
                                                           cgcgTGCGCCACGCTAAAAGCAGCCCGGAACGCCATCGATTAAAGTACTGGCTAAACGTGCTTATAATAAAAAAGCTAAAGACAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCaa                                                                                            <  1:3226939/150‑1 (MQ=255)
                                                           cgcgTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCaa                                                                                            <  2:1612602/150‑1 (MQ=255)
                                                            gcgTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAg                                                                                           <  2:2833867/150‑1 (MQ=255)
                                                             cgTGCGCCAAGCGAAAAGCAGACCGGAAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGc                                                                                          <  2:3170746/150‑1 (MQ=255)
                                                              gTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAATAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCg                                                                                         <  1:1001423/150‑1 (MQ=255)
                                                              gTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCg                                                                                         >  2:361481/1‑150 (MQ=255)
                                                                  gcCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAACAATATTGGTAAGCGCCGTAAACCAAGCGGacc                                                                                     >  2:11697245/1‑148 (MQ=255)
                                                                  ccaaaGCTAAAAGCAGACCGGCAAGCCTTCTAAGAATGTACTGGATAAACGTGCTGAGAACAAAAAAGCTAAAGCGAAAGAGACGCGGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCACGCGGAAc                                                                                     <  1:9254287/147‑1 (MQ=255)
                                                                    cAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACgg                                                                                   >  1:6604053/1‑150 (MQ=255)
                                                                    cAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACgg                                                                                   <  2:9181241/150‑1 (MQ=255)
                                                                    cAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACgg                                                                                   <  2:11154190/150‑1 (MQ=255)
                                                                     aaGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACggg                                                                                  >  2:1336841/1‑150 (MQ=255)
                                                                     aaGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACggg                                                                                  >  1:461450/1‑150 (MQ=255)
                                                                     aaGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACggg                                                                                  >  1:9913140/1‑150 (MQ=255)
                                                                     aaGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACggg                                                                                  >  1:1927892/1‑150 (MQ=255)
                                                                     aaGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACggg                                                                                  >  2:1144173/1‑150 (MQ=255)
                                                                      aGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGc                                                                                 >  2:8025079/1‑150 (MQ=255)
                                                                         gAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGACCGGGCGTg                                                                              >  1:8655852/1‑150 (MQ=255)
                                                                         gAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTg                                                                              >  2:12066013/1‑150 (MQ=255)
                                                                          aaaaGCATACCGGCAAGCCATCTAATAAAGTACTGGCTAAACGTGCTGATAAGAAAAAAGCTAAAGAGAAAGACACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGc                                                                             <  1:9981511/150‑1 (MQ=255)
                                                                             agcagcCCGGCAAGCCATCGAATAAAGTACTGGCTAAAGGTGCTGATAATAAAAAAGCTAAAGAGACAGCCACGCCGCGGGGGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCAc                                                                          <  1:10255294/150‑1 (MQ=255)
                                                                               cAGACCGGCAAGCCATCCAAGAAAGAACTCGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAACCAGAAGCCGCGGGTCAAAAAACGCCATCGCGACACCAAAAAAATTCGTCAGCGCCGAAAACCAAGCGGAACGGGCGTGCCCCCg                                                                        >  2:9787771/1‑150 (MQ=255)
                                                                                aGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGc                                                                       >  1:3578472/1‑150 (MQ=255)
                                                                                aGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGACACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGc                                                                       <  1:2828609/150‑1 (MQ=255)
                                                                                   ccGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCaaa                                                                    <  2:445134/150‑1 (MQ=255)
                                                                                     ggCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGACCGGGCGTGCCACCGCAAACg                                                                  >  2:6068638/1‑150 (MQ=255)
                                                                                          gCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACaga                                                             >  2:11308655/1‑150 (MQ=255)
                                                                                             aTCTAATAATGTACTGGATAAACGTGCTGATAAGAAAAAATCTAAAGATAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          <  1:6922377/150‑1 (MQ=255)
                                                                                             aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  1:9482536/1‑150 (MQ=255)
                                                                                              tCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAagag                                                         >  2:585393/1‑150 (MQ=255)
                                                                                               cGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGt                                                        >  2:172836/1‑150 (MQ=255)
                                                                                               cGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGt                                                        >  1:601602/1‑150 (MQ=255)
                                                                                                gaagaaAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTCAGCGCCGTAAACCACGCGGAACGGGCGTGCCACCGCAAACGACAGACGAGtc                                                       >  2:3404823/1‑149 (MQ=255)
                                                                                                   gaaAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtc                                                    >  2:7813031/1‑150 (MQ=255)
                                                                                                    aaaGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtct                                                   >  1:8089185/1‑150 (MQ=255)
                                                                                                    aaaGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtct                                                   <  2:8174669/150‑1 (MQ=255)
                                                                                                        tACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAAt                                               >  1:3908040/1‑150 (MQ=255)
                                                                                                        tACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAAt                                               >  1:4808570/1‑150 (MQ=255)
                                                                                                           tGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGcc                                            >  1:6430854/1‑150 (MQ=255)
                                                                                                           tGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGcc                                            >  2:7527672/1‑150 (MQ=255)
                                                                                                             gCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAg                                          >  1:2708232/1‑150 (MQ=255)
                                                                                                                   cGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTaa                                    <  2:7128786/150‑1 (MQ=255)
                                                                                                                        tGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTg                               >  1:7419801/1‑150 (MQ=255)
                                                                                                                          agaagaAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACC‑AAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTa                            >  1:9746500/1‑150 (MQ=255)
                                                                                                                          agaagaAAAAAGCTAAAGACACCGCGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGt                             <  1:11549526/150‑1 (MQ=255)
                                                                                                                              gaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTa                         >  1:11143526/1‑150 (MQ=255)
                                                                                                                              gaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTa                         >  2:2175381/1‑150 (MQ=255)
                                                                                                                              gaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTa                         >  2:8827244/1‑150 (MQ=255)
                                                                                                                                 aaaaGCTAAAGAGAACGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaaaa                      <  2:10115360/150‑1 (MQ=255)
                                                                                                                                 aaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaaaa                      <  1:11821166/150‑1 (MQ=255)
                                                                                                                                 aaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATGaaaa                      >  2:6967689/1‑150 (MQ=255)
                                                                                                                                     gCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACC‑AAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCt                 <  1:9066158/150‑1 (MQ=255)
                                                                                                                                      cTAACGAGACAGAGAAGCCGCGGGGGAAAACACGCCATCGCGACACCCACACTATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCt                 <  2:1462892/150‑1 (MQ=255)
                                                                                                                                       tAAAGAGAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTg                <  1:7627736/150‑1 (MQ=255)
                                                                                                                                       tAAAGAGAAAGAGAAGCCGCGGGTGAAAAAATGCCATCGCGACACC‑AAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCACTGCCAGGTTTAAGCCTGGTCTTAAAAGTGCTgg               >  1:12249839/1‑150 (MQ=255)
                                                                                                                                        aaagagaaagagAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTgg               <  1:6850225/150‑1 (MQ=255)
                                                                                                                                        aaagagaaagagAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTgg               >  2:7419039/1‑150 (MQ=255)
                                                                                                                                        aaagagaaagagAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTgg               >  1:2505696/1‑150 (MQ=255)
                                                                                                                                         aagagaaagagaAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGc              >  2:8398470/1‑150 (MQ=255)
                                                                                                                                         aagagaaagagaAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGc              <  1:8918941/150‑1 (MQ=255)
                                                                                                                                            cgaaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATCTTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCaaa           <  1:10627764/149‑1 (MQ=255)
                                                                                                                                            agaaagcgacGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCCAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCaaa           <  1:543216/150‑1 (MQ=255)
                                                                                                                                            agaaagagacGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCaaa           <  1:10927754/150‑1 (MQ=255)
                                                                                                                                            agaaacacaaCCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCAACCGCAAACGACAGAAGATTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCaaa           <  1:2281640/150‑1 (MQ=255)
                                                                                                                                             gaaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAACTATGGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAAc          <  2:10855472/150‑1 (MQ=255)
                                                                                                                                                     aGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACt  <  2:4823715/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACT  >  NC_000913/2713984‑2714282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑