BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,990,927	T→A	5.2%	pseudogene (374/396 nt)	ygeF →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,990,927	0	T	A	5.2%	1770.3 / 9.0	531	pseudogene (374/396 nt)	ygeF	pseudogene
Reads supporting (aligned to +/- strand): ref base T (277/215); new base A (5/27); total (283/248)
Fisher's exact test for biased strand distribution p-value = 5.65e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 536 total aligned reads displayed.

ATTATCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTA  >  NC_000913/2990778‑2991076

 ttatCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTc                                                                                                                                                      >  2:4367680/1‑150 (MQ=255)

     cTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCAGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGACACCa                                                                                                                                                  >  2:7151977/1‑150 (MQ=255)

      tGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAg                                                                                                                                                 >  1:2868409/1‑150 (MQ=255)

       gCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGa                                                                                                                                                <  2:247348/150‑1 (MQ=255)

        cTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGAc                                                                                                                                               <  1:5060206/150‑1 (MQ=255)

        cTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGAc                                                                                                                                               <  1:9087798/150‑1 (MQ=255)

            gAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACataa                                                                                                                                           <  1:6934804/150‑1 (MQ=255)

               cATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACAtaataa                                                                                                                                        >  2:4077486/1‑150 (MQ=255)

                atatatAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATaaa                                                                                                                                       <  2:4797087/150‑1 (MQ=255)

                atatatAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATaaa                                                                                                                                       <  2:4553731/150‑1 (MQ=255)

                 tatataAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAg                                                                                                                                      <  2:963232/150‑1 (MQ=255)

                   tataAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaa                                                                                                                                    <  1:4299755/150‑1 (MQ=255)

                    ataAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaa                                                                                                                                   >  2:9762608/1‑150 (MQ=255)

                    ataAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaa                                                                                                                                   >  1:6353233/1‑150 (MQ=255)

                    ataAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaa                                                                                                                                   >  2:5679215/1‑150 (MQ=255)

                     taAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaaa                                                                                                                                  >  1:11053043/1‑150 (MQ=255)

                     taAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTAATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaaa                                                                                                                                  <  2:11887153/150‑1 (MQ=255)

                        aTTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaaaaaa                                                                                                                               <  1:11707383/150‑1 (MQ=255)

                        aTTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGaaaaaaa                                                                                                                               <  2:5822697/150‑1 (MQ=255)

                         ttttCGATAACAGATGATTTAATGAGTATGGCTGTAAAAGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAaaac                                                                                                                              <  2:6545803/150‑1 (MQ=255)

                            tCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAaaacaca                                                                                                                           <  1:5239817/150‑3 (MQ=255)

                            tCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAaaacaaa                                                                                                                           <  2:82616/150‑1 (MQ=255)

                              gATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATGAAGAAAAAAACAAACg                                                                                                                         >  1:2549669/1‑150 (MQ=255)

                                tAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGAc                                                                                                                       >  1:3884170/1‑150 (MQ=255)

                                    aGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTc                                                                                                                   >  1:8220034/1‑150 (MQ=255)

                                     gatgatTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATTGAGAATATTGTAGACCTGAATATATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATATTAAAGAAAAAAACAAACGACATTCt                                                                                                                  <  2:9167240/150‑1 (MQ=255)

                                     gatgatTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCt                                                                                                                  >  2:5315969/1‑150 (MQ=255)

                                     gatgatTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCt                                                                                                                  >  1:500096/1‑150 (MQ=255)

                                         atTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTAccc                                                                                                              >  1:5748185/1‑150 (MQ=255)

                                             aaTGATTATGGTTTTAAATGAAAAAATAAAAATTACAAAAATAGACAATAATGTAGACCTGAATAAATTAACTACTATATGCACATACGTTCTTTCCCCCCGCGTCACAAGAAATAATAAAGAAAAAAACAAACGACATTCTACCCTtct                                                                                                          <  2:10698271/150‑1 (MQ=255)

                                                gAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCaa                                                                                                       >  2:2353346/1‑150 (MQ=255)

                                                  gTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCaaaa                                                                                                     <  2:4019011/150‑1 (MQ=255)

                                                       gttttAAATGAACTCTTAAATCTGACCAAAATTGTGATTATTGTAGACCTGAATAATTTCACTACTTTTTGCACTGAGGTTCTATCCCCCGGCGTCACCAGACATAATAAATAAAAAAACAAACGACATTCTACCCTTCTCAAAAATccc                                                                                                <  2:9389567/146‑1 (MQ=255)

                                                         tGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCt                                                                                              >  1:3775779/1‑150 (MQ=255)

                                                         tGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCt                                                                                              >  2:1218313/1‑150 (MQ=255)

                                                         tGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCt                                                                                              >  2:10846421/1‑150 (MQ=255)

                                                         tGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCt                                                                                              >  1:9960571/1‑150 (MQ=255)

                                                           tAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCttt                                                                                            >  1:2308916/1‑150 (MQ=255)

                                                             aaTGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCttttt                                                                                          >  1:1075802/1‑150 (MQ=255)

                                                               tGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTaa                                                                                        >  1:1810804/1‑150 (MQ=255)

                                                               tGAACAAATAAAACTGACAAAAATTCATAATATTTTAGACCTGTATAAATACACTACTATATGCACAGAGGTTCTATCCCCCCGCGTCACCAGAAATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTaa                                                                                        <  2:11340136/150‑1 (MQ=255)

                                                                gAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAt                                                                                       >  1:246117/1‑150 (MQ=255)

                                                                  aCTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAAttt                                                                                     <  2:7020872/150‑1 (MQ=255)

                                                                    tCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTc                                                                                   >  1:10438870/1‑150 (MQ=255)

                                                                     aaTAAAACTCACAAAAATACATAATAATGTAGACATGAATAAATACAATACTATATGCAAAGAGGTTCCACCCCCCCGCGTCACCAGAAATAAAAAACAAAAAAACACACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCa                                                                                  <  1:8658275/149‑1 (MQ=255)

                                                                      aTAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCAt                                                                                 >  1:9639305/1‑150 (MQ=255)

                                                                      aTAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCAt                                                                                 >  2:2162660/1‑150 (MQ=255)

                                                                       tAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCAtt                                                                                >  1:3635468/1‑150 (MQ=255)

                                                                           aCTGACAAAAATAGAGAATAATGTAGACCTGAATAAATTCACTAATATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGaaa                                                                            <  2:2837495/150‑1 (MQ=255)

                                                                               acaaaaaTATATAATATTTTAGACCTTAATAAATTCACTAATATATGCACAGACGTTCTACCCCCCCGCGACACCACAAATAATAAATAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAAt                                                                        <  1:6166019/147‑1 (MQ=255)

                                                                               aCCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAAt                                                                        >  2:6782902/1‑150 (MQ=255)

                                                                                 cAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATgg                                                                      >  1:10035031/1‑150 (MQ=255)

                                                                                 cAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATgg                                                                      >  2:8119013/1‑150 (MQ=255)

                                                                                   aaaTAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCa                                                                    >  1:11915058/1‑150 (MQ=255)

                                                                                    aaTAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCaa                                                                   >  1:6779926/1‑150 (MQ=255)

                                                                                      tAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAAtt                                                                 >  1:1504705/1‑150 (MQ=255)

                                                                                      tAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAAtt                                                                 >  2:4190906/1‑150 (MQ=255)

                                                                                        gataataatGTAGACCTGAATAAATTCACTACTATATGCACAGACTTTCTACCCCCCCGCGACACCAGAAATAATAAATAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGa                                                               <  1:2726304/147‑1 (MQ=255)

                                                                                          gaATAATGTAGACCTGAATAGATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATa                                                             <  2:1396422/150‑1 (MQ=255)

                                                                                          caataatTTACACATTAAAAAATACACTACTATATGCACAGACGTTCAAACCCCCCGCGACACCACAAAAAAAAAAAAAAAAAACACACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATa                                                             <  1:11325203/149‑1 (MQ=255)

                                                                                          caataatGTAGACCTGAATAAATTCACTACTATATGCACAGACGTACTATCCCCCCGCGTCACCACACATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATa                                                             <  2:3199749/149‑1 (MQ=255)

                                                                                           aataatGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGACACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCATTTTAATTTTCATTGAAAAAATGGAAATTGATAc                                                            >  2:1559947/1‑150 (MQ=255)

                                                                                             taatTTAGACCTTAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTAACCACACATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTa                                                          <  2:10577020/150‑1 (MQ=255)

                                                                                             taatGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTa                                                          >  1:5380551/1‑150 (MQ=255)

                                                                                             taatGTAGACCTGAATAAATTCACTACTATATGAACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTa                                                          <  2:1710617/150‑1 (MQ=255)

                                                                                                  tAGACCTTAATAAATTCACTAATATATGCACAGACGTTCTATCCCCCCGCGTCACCAGAAATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAAt                                                     <  2:8959664/150‑1 (MQ=255)

                                                                                                    gACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATcc                                                   >  2:8007844/1‑150 (MQ=255)

                                                                                                     aCCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCa                                                  >  1:4533328/1‑150 (MQ=255)

                                                                                                       cTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAgg                                                >  2:6053040/1‑150 (MQ=255)

                                                                                                        tGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGt                                               <  2:5082336/150‑1 (MQ=255)

                                                                                                        tGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGt                                               >  2:2077839/1‑150 (MQ=255)

                                                                                                        tGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGt                                               >  2:1925705/1‑150 (MQ=255)

                                                                                                            tAAATACACTACTATATGCACAGACGTTCTCCCCCCCCGCGTCACCCCACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATa                                           <  1:7111224/150‑1 (MQ=255)

                                                                                                             aaaTTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATac                                          <  1:11179939/150‑1 (MQ=255)

                                                                                                                    ctactaTATGCACAGACGTTCTATCCCCCCGCGTCACCACACATAATAAACAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTaa                                   <  2:6234558/150‑1 (MQ=255)

                                                                                                                    atactaTATGCACAGACGTTCTATCCCCCCGCGTCACCAGAAATATAAAATAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTaa                                   <  1:9364626/149‑1 (MQ=255)

                                                                                                                     tactaTATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATACAGGTCATACACATAAAg                                  >  2:11190659/1‑150 (MQ=255)

                                                                                                                        tataTGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCa                               <  2:11053043/150‑1 (MQ=255)

                                                                                                                             gCACAGACTTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTAt                          <  2:8694654/150‑1 (MQ=255)

                                                                                                                             gCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTAt                          >  1:6550795/1‑150 (MQ=255)

                                                                                                                             gCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTAt                          <  2:11145791/150‑1 (MQ=255)

                                                                                                                              cacaGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATg                         >  1:881187/1‑150 (MQ=255)

                                                                                                                              cacaGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATg                         >  2:2989822/1‑150 (MQ=255)

                                                                                                                               acaGAGGTGCTAGCCCCCCGCGTCACCAGAAATAATAACGAAAAAAACACACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGt                        <  2:1163592/150‑1 (MQ=255)

                                                                                                                                caGCCGTTCTAGCCCCCCGCGCCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTa                       <  2:9366866/150‑1 (MQ=255)

                                                                                                                                caGACGTTCTCTCCCCCCGCGTCACCAGACATAATAAATAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTa                       <  1:9509925/150‑1 (MQ=255)

                                                                                                                                caGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTa                       <  1:735045/150‑1 (MQ=255)

                                                                                                                                 aGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTaa                      >  1:7705353/1‑150 (MQ=255)

                                                                                                                                    cGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGTTACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGt                   <  2:3431615/150‑1 (MQ=255)

                                                                                                                                         tATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTaa              >  2:7424259/1‑150 (MQ=255)

                                                                                                                                         tATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTaa              >  1:807079/1‑150 (MQ=255)

                                                                                                                                          aTCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAAt             >  1:2044415/1‑150 (MQ=255)

                                                                                                                                          aTCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAAt             >  1:11800012/1‑150 (MQ=255)

                                                                                                                                           tCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCAAGGTCATACACATTAAGGCATTTATGTAATGTCGTAAtt            >  2:12099498/1‑150 (MQ=255)

                                                                                                                                            ccccccGCGGCACCAGACAGAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAAGGGCAATTGATACTCTGAATCCAGGTCATACACAGTAAGGCAGGTCGGTAATGTCGTACTTa           >  2:10122214/1‑150 (MQ=255)

                                                                                                                                             cccccGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTaa          >  1:7494844/1‑150 (MQ=255)

                                                                                                                                              ccccGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAg         >  1:4068134/1‑150 (MQ=255)

                                                                                                                                              ccccGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAg         <  2:879874/150‑1 (MQ=255)

                                                                                                                                               cccccctCACCAGACAGAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAAAGGCAAAAGATACTAAGAAACCAGGTCAAACACATAAAGGCCTTTATGTAAAGTCGTAAAAAAGa        >  2:6845692/7‑150 (MQ=255)

                                                                                                                                               cccGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAgt        >  2:2619628/1‑149 (MQ=255)

ATTATCTGCTATGAGCATATATAAATTTTCGATAACAGATGATTTAATGAGTATGGCTGTAAATGAACTCATAAAACTGACCAAAATAGAGAATAATGTAGACCTGAATAAATTCACTACTATATGCACAGACGTTCTATCCCCCCGCGTCACCAGACATAATAAAGAAAAAAACAAACGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTA  >  NC_000913/2990778‑2991076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`