Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,671 A→C 7.1% intergenic (+22/+80) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6710AC 7.1% 2175.7 / 13.3 697intergenic (+22/+80)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (386/224);  new base C (2/70);  total (388/309)
Fisher's exact test for biased strand distribution p-value = 1.39e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 716 total aligned reads displayed.

GGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCC  >  NC_000913/3608522‑3608820
                                                                                                                                                     |                                                                                                                                                     
ggCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGa                                                                                                                                                       >  1:6597480/1‑150 (MQ=255)
ggCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGa                                                                                                                                                       <  1:6497919/150‑1 (MQ=255)
ggCTGAAAGGGATCGTCCTCGTCACCCCGCTGTTAGGGATGCCCGGGTTGTGGCTGGCAGTGCGGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGTTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGa                                                                                                                                                       <  1:1327409/150‑1 (MQ=255)
   tGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAgcg                                                                                                                                                    >  2:8093010/1‑150 (MQ=255)
   tGAAACGGATATACCACGAAACCACGCTGTAACGGATCACCCCCTGCTGGCTTGCAGAGCAGGCAGATACGGGTGCGACGGTGCTGTTGACAGCGAATACGTTAAGATTGTTGCGCAGGAGATAATGCAAACCGATCGCAACATTGAgcg                                                                                                                                                    >  2:10578016/1‑150 (MQ=255)
      aaGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGa                                                                                                                                                 <  1:7440065/150‑1 (MQ=255)
              ttCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTccc                                                                                                                                         >  1:3146321/1‑150 (MQ=255)
               tCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTcccc                                                                                                                                        >  2:8274713/1‑150 (MQ=255)
                 cTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTc                                                                                                                                      >  2:9225296/1‑150 (MQ=255)
                 cTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGATAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGCTCCCCTc                                                                                                                                      >  1:5610964/1‑150 (MQ=255)
                  tCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCg                                                                                                                                     >  1:1503083/1‑150 (MQ=255)
                  tCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCg                                                                                                                                     >  1:6117497/1‑150 (MQ=255)
                          acGCTGTTAGGGATGACCGGGTTGTGGCTGGCTGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                             <  1:8811842/150‑1 (MQ=255)
                          acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                             <  2:10076238/150‑1 (MQ=255)
                           cGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                            <  2:4919497/150‑1 (MQ=255)
                            gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCgggg                                                                                                                           >  1:4815069/1‑150 (MQ=255)
                            gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                           <  1:536082/150‑1 (MQ=255)
                            gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                           <  2:3225137/150‑1 (MQ=255)
                            gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                           <  2:1635579/150‑1 (MQ=255)
                              tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                         >  1:11972905/1‑150 (MQ=255)
                              tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                         >  1:11679135/1‑150 (MQ=255)
                              tGTTAGGGATGACCGGGGTGTGGCTGGCAGTGCGGGCCGATCCGGGGGCGCCGGTGCTGGTGACCGCGAATGCGTTAAGATGGGTGCGCAGGAGATAAGGCAACCCGATCGCAACATTGAGCGCGATGGGTCCCCTCGCCCCTCTGGGga                                                                                                                         <  1:11320207/150‑1 (MQ=255)
                                ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                       <  1:11515214/150‑1 (MQ=255)
                                 tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                      >  2:224024/1‑150 (MQ=255)
                                 tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                      <  1:4637902/150‑1 (MQ=255)
                                    ggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                   >  1:221831/1‑150 (MQ=255)
                                    ggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                   >  1:6700439/1‑150 (MQ=255)
                                    ggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                   >  2:10262797/1‑150 (MQ=255)
                                    ggATGACCGGGGTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGAGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                   >  1:4236379/1‑150 (MQ=255)
                                     gATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCTCCCCAGAGGGGCGAgggga                                                                                                                  <  2:3456763/150‑3 (MQ=255)
                                        gACCGGGTTGTGGCTGGCAGTTCTGGCAGATTCGGGGGCGACGTTGCTGGTGACAGCTAATTCGTTAAGATTGTTGCGCTGGAGATAAGGCAACCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                               <  1:2785146/150‑1 (MQ=39)
                                         aCCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                              >  1:1727081/1‑150 (MQ=255)
                                          ccGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCTCCCCAGAGGGGCGAggggaccgat                                                                                                             <  2:4159637/150‑8 (MQ=255)
                                           cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                                                            >  1:4053065/1‑150 (MQ=255)
                                           cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                                                            >  1:7857932/1‑150 (MQ=255)
                                            gggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                                                                           >  1:10603121/1‑150 (MQ=255)
                                             ggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                          <  1:11184684/150‑1 (MQ=255)
                                              gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                         >  1:10006321/1‑150 (MQ=255)
                                                    gctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCACACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                   <  2:1365118/150‑1 (MQ=255)
                                                      tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                 >  1:719026/1‑150 (MQ=255)
                                                      tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                 >  1:4479918/1‑150 (MQ=255)
                                                         cAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcgg                                                                                              >  2:7036023/1‑150 (MQ=255)
                                                          aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGACAAggcggc                                                                                             >  1:8358380/1‑150 (MQ=255)
                                                           gTGCTGGCAGTTAGGGGGGCGAGGGTGCTGGTGACAGCGAATGCGTTATGTTTGTGGCGCTGTATATAAGGAAAACCGATCGAAACTTTGTGCGCGATCGTTCCCCTCGCCCCTCTGGGTTGAGGTTTAGGGTGAGGGGAAAAGGCGGCa                                                                                            <  2:976092/150‑1 (MQ=255)
                                                           gTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCa                                                                                            >  1:10837851/1‑150 (MQ=255)
                                                            tGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCAt                                                                                           >  1:6950443/1‑150 (MQ=255)
                                                                 gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                      >  2:6124140/1‑150 (MQ=255)
                                                                  cagagccGGGGGCGAGGGTGCTGGTGACTGCGATTGCGTTAAGTTTGTTGCGCAGTTGATAAGGCAAACCGATCGCAACTTTGAGCGCGATGGTTCCCCTCGCCCCTCTGGGGAGCGGTTTAGGGTGAGGGGAAAAGGCGGCATCGAAGc                                                                                     <  2:11056617/144‑1 (MQ=37)
                                                                   aGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGcc                                                                                    >  1:8177170/1‑150 (MQ=255)
                                                                        cGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCa                                                                               >  2:3998426/1‑150 (MQ=255)
                                                                           gggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGcc                                                                            >  1:12215618/1‑150 (MQ=255)
                                                                            ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGATATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                           <  2:5675547/150‑1 (MQ=255)
                                                                               gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTa                                                                        >  1:8971757/1‑150 (MQ=255)
                                                                                    tGCTGGTTACCGCCTATGGTTTAAGTTTGTTGCGCTTGATATACGCCACCCCCATCGCAAAATTGAGCGCCAGGGTCCCCCTCGCCCCTCTGGGGTGTGGTTTTGGTTGCGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAc                                                                   <  2:7233342/150‑1 (MQ=255)
                                                                                    tGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTACCAAc                                                                   >  1:11048647/1‑150 (MQ=255)
                                                                                       tGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                >  2:2813464/1‑150 (MQ=255)
                                                                                          tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                             >  1:9507621/1‑150 (MQ=255)
                                                                                            aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                           >  1:3578857/1‑150 (MQ=255)
                                                                                               gctattGCGTTATGTTTGGTGCGCCGGAGATACGGCAACCCGAACGAAACATTGAGCGCGATCGGTCCCCCCGCCCCTGGGGGTAGCGGTTTTGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                        <  1:10170410/145‑1 (MQ=255)
                                                                                                 gAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                      >  2:2639892/1‑150 (MQ=255)
                                                                                                    tGCTTTAAGATTGTTGCGCAGTAGATACGGCAACCCGACCGCAACTTTGCGCGCGATCGGCCCCCCCGCCCCTCTGGGGAGTGGTTTCGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaa                                                   <  2:107980/150‑1 (MQ=255)
                                                                                                       gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                >  1:10278295/1‑150 (MQ=255)
                                                                                                       gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                >  2:9647785/1‑150 (MQ=255)
                                                                                                       gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                >  2:10474850/1‑150 (MQ=255)
                                                                                                        ttAAGTTTGTGGCGCAGGATATACGGCCAACCGATCGCAACATTGCGCGCGAGCGGTCCCCTCGCCCCTCTGGGGTGTGGTTTAGGGTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                               <  2:10638263/150‑1 (MQ=255)
                                                                                                          aaGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACg                                             >  2:5032912/1‑150 (MQ=255)
                                                                                                          aaGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACg                                             >  2:3647463/1‑150 (MQ=255)
                                                                                                             aTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCTGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATa                                          >  2:11128982/1‑150 (MQ=255)
                                                                                                             aTTGTTGCGCAGGAGATAAGGCAAACCGAACGCAACAATGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCCTCGAAGCCAATCAGCCCCTATCAACCGCCGTTTCGCAGCAAATAACGATa                                          >  2:12193841/1‑150 (MQ=38)
                                                                                                              ttgttgCGCAGGAGATACGGCAACCCGATCGCAACATTGAGCGCGATGGGTCCCCTCGCCCCTGTGGGTAGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                         <  2:11972905/150‑1 (MQ=255)
                                                                                                                gttgCTCTGTAGATACGGCAACCCGATCGAAACTTTGCGCGCGATCGGCCCCCGCGCCCCTGGGGGGTGTGGTTTAGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAgg                                       <  2:5498594/150‑1 (MQ=255)
                                                                                                                  tgCGCTGTATATAAGGCAAACCGATCGAAACTTGGTGCGCTATGGGTCCCCTCGCCCCTGTGGGGTGTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCa                                     <  2:11595881/150‑1 (MQ=255)
                                                                                                                    ctcAGGATATAAGGCAAACCTATCGAAAATTTGAGCGCGATCGGTCCCCTCGCCCCTGTGGGGAGTGTGTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGt                                   <  2:7776274/148‑1 (MQ=255)
                                                                                                                      cAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTcc                                 >  2:8937125/1‑150 (MQ=255)
                                                                                                                      cAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTcc                                 >  1:977701/1‑150 (MQ=255)
                                                                                                                         gagaTACGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATcc                             <  2:4356347/150‑1 (MQ=255)
                                                                                                                         gagaTAAGGCAAACGTATCGCAACATGGCGCGCGTTCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTTGGGTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATc                              <  2:6826555/150‑1 (MQ=38)
                                                                                                                            aTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGt                           >  2:1969180/1‑150 (MQ=255)
                                                                                                                                ggCAACCCGATCGAAACATTGAGCGCGTTCGGGCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                       <  2:6540383/150‑1 (MQ=39)
                                                                                                                                 gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                      >  2:3040912/1‑150 (MQ=255)
                                                                                                                                 gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                      >  1:2524220/1‑150 (MQ=255)
                                                                                                                                  cAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                     >  2:4654993/1‑150 (MQ=255)
                                                                                                                                   aaaCCGAGCGCAACATTGAGCGCGATCGGCCCCCTCGCCCCTCGGGGGAGAGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                    <  2:7385142/150‑1 (MQ=255)
                                                                                                                                       cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                >  1:4988720/1‑150 (MQ=255)
                                                                                                                                       cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                >  1:3927897/1‑150 (MQ=255)
                                                                                                                                       cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                >  2:9446158/1‑150 (MQ=255)
                                                                                                                                       cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                >  2:7520580/1‑150 (MQ=255)
                                                                                                                                            gCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGt           >  1:9946110/1‑150 (MQ=255)
                                                                                                                                            gCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGt           >  1:779235/1‑150 (MQ=255)
                                                                                                                                            gCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGt           >  1:3820877/1‑150 (MQ=255)
                                                                                                                                             cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAATGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt          >  2:3870413/1‑150 (MQ=255)
                                                                                                                                             cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt          >  1:3411805/1‑150 (MQ=255)
                                                                                                                                             cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt          >  1:2948215/1‑150 (MQ=255)
                                                                                                                                             cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt          >  2:5961115/1‑150 (MQ=255)
                                                                                                                                             cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt          >  2:629235/1‑150 (MQ=255)
                                                                                                                                                  ttGCGCGCGTTCGGCCCCCTCGCCCCTGTGGGGTGCGGGTTAGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt     <  1:8672153/150‑1 (MQ=255)
                                                                                                                                                   tGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGtt    >  1:8521967/1‑150 (MQ=255)
                                                                                                                                                   tGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGtt    >  2:8399310/1‑150 (MQ=255)
                                                                                                                                                    gAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTc   <  1:9074500/150‑1 (MQ=255)
                                                                                                                                                     aGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc  >  2:9067898/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCC  >  NC_000913/3608522‑3608820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: