Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,695 C→G 12.0% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6950CG12.0% 2300.7 / 98.1 746intergenic (+46/+56)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (398/245);  new base G (5/95);  total (403/343)
Fisher's exact test for biased strand distribution p-value = 1.45e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 768 total aligned reads displayed.

CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAAT  >  NC_000913/3608546‑3608843
                                                                                                                                                     |                                                                                                                                                    
 cacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctct                                                                                                                                                     >  2:10539891/1‑150 (MQ=255)
 cacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctct                                                                                                                                                     >  1:11595881/1‑150 (MQ=255)
  acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                                                    <  1:3942628/150‑1 (MQ=255)
   cGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                   <  1:11392693/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                  <  1:9071523/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                  <  2:11109464/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                  <  1:1379554/150‑1 (MQ=255)
     cTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGTTACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgggg                                                                                                                                                 <  1:2388328/150‑1 (MQ=255)
      tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                >  1:4056427/1‑150 (MQ=255)
      tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                >  1:7309225/1‑150 (MQ=255)
      tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                <  1:11232702/150‑1 (MQ=255)
      tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                >  2:3609797/1‑150 (MQ=255)
         tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                             >  2:224024/1‑150 (MQ=255)
         gaGGGAGGCCCGGGGTGGGGCTGGCAGTGCTGGCAGAGCCGGGGGCGCCGGTGCTGGTGACCGCGACTGCGTTAAGATTGGGGCGCAGGAGATAAGGCAAACCGCTCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCGCTGGGGagag                                                                                                                                             <  2:3860438/149‑1 (MQ=255)
          aGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                                                                            >  2:11455887/1‑150 (MQ=255)
           gggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCTCCCCAGAGGGGCGAggg                                                                                                                                           <  1:7867394/150‑1 (MQ=255)
             gATGACCGGGTTGTGGCTGGCAGTGCTGGCAGTTACGGGGGCGACGGTGCTGGTGACAGCGAAGGCGTTAAGATTGTTGCGCTGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGTTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                                                                                         <  1:10809293/150‑1 (MQ=255)
                 aCCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                     >  1:4311474/1‑150 (MQ=255)
                  ccGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGt                                                                                                                                    >  1:6631875/1‑150 (MQ=255)
                   cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                                                                                   >  1:9758150/1‑150 (MQ=255)
                     ggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                 <  2:9271632/150‑1 (MQ=255)
                      gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGCTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                <  1:28956/150‑1 (MQ=255)
                      gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATACGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                <  1:3495422/150‑1 (MQ=255)
                      gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                >  1:10006321/1‑150 (MQ=255)
                            gctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                                          <  1:484223/150‑1 (MQ=255)
                              tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                        >  1:4479918/1‑150 (MQ=255)
                                  aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcggc                                                                                                                    >  2:4694045/1‑150 (MQ=255)
                                   gTGCTGGCAGTTAGGGGGGCGAGGGTGCTGGTGACAGCGAATGCGTTATGTTTGTGGCGCTGTATATAAGGAAAACCGATCGAAACTTTGTGCGCGATCGTTCCCCTCGCCCCTCTGGGTTGAGGTTTAGGGTGAGGGGAAAAGGCGGCa                                                                                                                   <  2:976092/150‑1 (MQ=255)
                                        ggCAGATAGGGGGGCGCGGGTGCTGGTGACTGCTAATGCGTTAAGTTTGTTGCGCTGGAGATAAGGAACACCGATGGCAACATTGAGCGGGTTGGGTCCCCTCGCCCCTCTGGGTTGTGGGTTTGGGTGTGGGTAAAAGGCGGCATCGaa                                                                                                              <  2:10063466/150‑1 (MQ=255)
                                         gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                             >  1:6108946/1‑150 (MQ=255)
                                          ctgATAGGGGGGCGCCGGTGCTGGTGACTGCGAATGCGTTAAGTTTGTTGCGCAGGAGATACGGCAAACCGATGGCAACATTGGGGGCGTTCGGTCCCCTCGCCCCTGTGGGTAGAGGGTTAGGGTGTGGGGAAAAGGCGGCATCGAAGc                                                                                                            <  2:7025719/148‑1 (MQ=255)
                                          ccgATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGACACGGCAAACCGATCGCAACATTGTGCGCGATCGGTCCCCTCGCCCCTGTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGc                                                                                                            <  2:12126110/148‑1 (MQ=255)
                                          cAGACAGGGGGGCGCCGGTGCTGGTGACAGCGATTGGTTTATTATTGTTGGTCAGGTTATACGGCAAACGGATCGCAAAATTGAGGGGTTTCGGTCCCCTCCCCCCTCTGGGGTGTGTTTTTGGGTTTGGGGAAAAGGCGGCATCGAAGc                                                                                                            <  2:4997792/150‑1 (MQ=255)
                                             ttccGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAATTTTGTTGCGCTGGATTTACGGCAACCCTTTCGCAACTTTGAGGGCTTTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGAGGGGAGAAGGCGGCATCGAAGCCaa                                                                                                         <  2:1983001/147‑1 (MQ=25)
                                             aTACGGGGGCGAGGGTGCTGGTGACAGCGAATGGTTTATGATTGTTGCGCAGGTGATAAGGCAAACCGATCGCAACATTGAGGGCGATGGGTCCCCTCGCCCCTCTGGGGTGTGGGTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCaa                                                                                                         <  2:4096667/150‑1 (MQ=38)
                                               cggggggCGCCGGTGCTGGTGACAGCTATTGCTTTATTATTGTTGCGCTGGAGATACGGAAAACCTATCGCAAATTTGCGCGCGATGGGCCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATc                                                                                                       <  2:2354937/148‑1 (MQ=25)
                                                  ggggCGCCGGTGCTGGTGACAGCGAATGCGTTAAGTTTGTTGCGCAGGAGATAAGGCAACCCGATCGCAACATTGAGCGGGATGGGTCCCCTCGCCCGTGTGGGGATCGGGTTTGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGc                                                                                                    <  2:10627326/150‑1 (MQ=255)
                                                  ggggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGc                                                                                                    >  2:4576543/1‑150 (MQ=255)
                                                    ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCTATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                  <  2:7949678/150‑1 (MQ=255)
                                                      cGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCt                                                                                                >  2:5641043/1‑150 (MQ=255)
                                                       gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTa                                                                                               >  1:3066381/1‑150 (MQ=255)
                                                               tGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                                       >  2:11675015/1‑150 (MQ=255)
                                                                 gTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCt                                                                                     >  1:210808/1‑150 (MQ=255)
                                                                  tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                    >  1:9507621/1‑150 (MQ=255)
                                                                      aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                >  2:3411393/1‑150 (MQ=255)
                                                                       gctattGCGTTATGTTTGGTGCGCCGGAGATACGGCAACCCGAACGAAACATTGAGCGCGATCGGTCCCCCCGCCCCTGGGGGTAGCGGTTTTGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                                               <  1:10170410/145‑1 (MQ=255)
                                                                       gCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                                               <  2:7462920/150‑1 (MQ=255)
                                                                            tgtgTTATGTTTGTTGCGCTGGAGATAAGCCAAACGGATCGCAACTTGGAGCGCGAGCGGGCCCCCCGCCCCTGTGGGGAGAGGGTTTGGGGGGGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaa                                                                          <  2:8278939/147‑1 (MQ=255)
                                                                            tGCTTTAATATTTTTGCGCAGGAGAAATGTCAAACCGATCGAAAATTTGCGCGCGATGGGTCCCCTCGCCCCTCTGGGGTGTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaa                                                                          <  1:8225506/150‑1 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                       >  2:3941498/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                       >  1:6841294/1‑150 (MQ=255)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                       >  1:10278295/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCCGGAGATAAGGCAAACCGATCGCTACATTGCGCGCGAGCGGTCCCCTCGCCCCTCGGGGGCGAGGGTTCGGGTGAGGGGAAAGGCGGCATCGAAGCCCATCAGCCCCTCTCACCCGCCTGGACGACTCAAATAAc                                                                     >  2:10369956/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                      >  1:8635421/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                      >  1:12081541/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                      >  1:8777707/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                      >  2:1612260/1‑150 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                      >  1:11777284/1‑150 (MQ=255)
                                                                                  atttttgttgCGCTGTTGATATGCCAACCCGATCGCAACTTTGCGCGGGTTGGGGCCCCTCGCCCCTCTGGGTTTTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACg                                                                    <  2:4349677/146‑1 (MQ=255)
                                                                                   tttttgttgCGCTGGATATAAGCCAAACCGATCGCAACTTTGAGCGCGATGGGCCCCCTCGCCCCTGTGGGGAGTGGTTTTGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGa                                                                   <  2:7675817/147‑1 (MQ=255)
                                                                                   aGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGACCCTATCAACCGCCTTTACGAATCAAATAACGa                                                                   >  1:7066838/1‑150 (MQ=255)
                                                                                      ttgttgCGCTGGAGATAAGGCAAACCTATCGCAACTTTGAGCGCGATGGGTCCCCTCGCCCCTGTGGGGTGTGGGTTAGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                <  2:886197/150‑1 (MQ=255)
                                                                                      ttgttgCGCAGGATATACGGCAAACCGATCGCAAATTTGAGCGGGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                <  1:7528474/150‑1 (MQ=38)
                                                                                             gctggAGATACGGCAACCCGATCGAAACTTTGCGGGGGATGGGGCCCCGCGCCCCGGTGGGGTGTGGGTTGGGGTGGGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTc                                                         <  1:1654336/147‑1 (MQ=255)
                                                                                                      aaGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                >  1:11163177/1‑150 (MQ=255)
                                                                                                        ggCAACCCGATCGAAACATTGAGCGCGTTCGGGCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                              <  2:6540383/150‑1 (MQ=39)
                                                                                                        ggCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                              >  2:5140943/1‑150 (MQ=255)
                                                                                                        gccAAACCGATCGCAAATTTGCGCGCGAGCGGCCCCCCCGCCCCGCTGGGTTGTGGGTTGGGTTGTGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                              <  2:3122964/148‑1 (MQ=255)
                                                                                                         gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                             >  1:10560358/1‑150 (MQ=255)
                                                                                                         gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                             >  2:3040912/1‑150 (MQ=255)
                                                                                                         gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                             >  2:9168597/1‑150 (MQ=255)
                                                                                                          cAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                            >  2:10759155/1‑150 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                           >  1:3831285/1‑150 (MQ=255)
                                                                                                                 aTCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAcc                                     >  2:9630042/1‑150 (MQ=255)
                                                                                                                 aTCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCGTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAcc                                     >  2:7250163/1‑150 (MQ=255)
                                                                                                                  tCGCACCATTGCGCGCGATCGGTCCCCTCGCCCCTCGGGGGTGTGGGTTGGGGGGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCa                                    <  2:1347143/150‑1 (MQ=255)
                                                                                                                    gCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGt                                  >  1:779249/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCTGCATCGAAGCCAATCAGCCACTATCAACCGCCTTTACGAATCAAAAAACGATAAGGCAGTCCAACCGTCTCTTTAGCAACCAGtg                                 >  2:2804043/1‑149 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                 >  2:629235/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                 >  1:8945549/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                 >  2:5961120/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                 >  1:3411577/1‑150 (MQ=255)
                                                                                                                      aaCTTTGAGCGCGAGCGGCCCCCACGCCCCTCGGGGGTGTGGGTTTGGGGGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTc                                <  1:8393835/150‑1 (MQ=255)
                                                                                                                         aTTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtg                             >  1:5141319/1‑150 (MQ=255)
                                                                                                                          gggcgcgcGATCGGGCCCCCCGCCCCCGTGGGGTGCGGGTTCGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                            <  1:4744718/146‑1 (MQ=255)
                                                                                                                           tgcgcgcGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGtt                           <  2:12277819/147‑1 (MQ=255)
                                                                                                                           tGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTCTCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATACGTCTCTGTCGCAACCAGTTCGTGtt                           >  1:6522800/1‑150 (MQ=39)
                                                                                                                           tGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGtt                           >  2:8399310/1‑150 (MQ=255)
                                                                                                                               cgcgTTCGGTCCCCTCGCCCCTCTGGTGTGTGGTTTCGGGTGCGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                       <  1:10993548/150‑1 (MQ=255)
                                                                                                                                  gATCGGCCCCCTCGCCCCTCTGGGTTGAGGGTTTGGGTGCGGGGAACAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                    <  1:5926682/150‑1 (MQ=255)
                                                                                                                                   agcGGCCCCCTCGCCCGTGTGGGTTGTGGGTTCGGGTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                   <  1:3685220/148‑1 (MQ=255)
                                                                                                                                   aTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                   >  2:9032728/1‑150 (MQ=255)
                                                                                                                                       gTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc               >  2:10980429/1‑150 (MQ=255)
                                                                                                                                        tCCCCTCGCCCCTCTGGGGTGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACa              <  2:10183796/150‑1 (MQ=255)
                                                                                                                                          cccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaa            >  1:538270/1‑150 (MQ=255)
                                                                                                                                           ccTCGCCCGTCTGGGGAGAGGGTTAGGGTTAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa           <  2:7087571/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc          >  1:9876077/1‑150 (MQ=255)
                                                                                                                                             tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAcc         >  2:6673127/1‑150 (MQ=255)
                                                                                                                                                ccccTCTGGGGTGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAg      <  2:6127327/150‑1 (MQ=255)
                                                                                                                                                   ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCACCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGaa   >  1:2702116/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAAT  >  NC_000913/3608546‑3608843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: