Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,044 T→A 20.5% intergenic (+27/‑171) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0440TA20.5% 2239.6 / 210.9 961intergenic (+27/‑171)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (114/599);  new base A (209/4);  total (356/605)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1007 total aligned reads displayed.

GTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTG  >  NC_000913/3780895‑3781193
                                                                                                                                                     |                                                                                                                                                         
  ggCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCctct                                                                                                                                                         <  2:8661694/150‑1 (MQ=255)
    cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCTCTcc                                                                                                                                                       >  1:4080042/1‑150 (MQ=255)
     gATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCACAccc                                                                                                                                                      >  1:2051316/1‑150 (MQ=255)
        gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                                                   <  1:6044538/150‑1 (MQ=255)
         aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                                 <  1:8670214/150‑1 (MQ=255)
          cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                                                <  1:5294416/150‑1 (MQ=255)
          cGCAGACGGGCGCGCAATCGAGGAGCGCAATGACGCAAGCTTCGCTGGTGCAGGGGCTGGGTCCAGAGTTGCCTGCGGGACTGGCTCCCATGGCGCAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAgg                                                                                                                                                <  2:8196687/150‑1 (MQ=255)
           gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCGAAACCACAcccaaaagg                                                                                                                                                >  1:4775869/1‑144 (MQ=255)
              gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgaga                                                                                                                                            <  1:6274150/150‑1 (MQ=255)
                   gcgcGAAATCGATCAGCGACAGTACGCAAGAATCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                                                      <  1:2930138/150‑1 (MQ=255)
                   gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                                                      <  1:8829389/150‑1 (MQ=255)
                   gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                                                      <  1:11787918/150‑1 (MQ=255)
                    cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggtta                                                                                                                                      <  2:1094572/150‑4 (MQ=255)
                    cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                                                     <  1:9019367/150‑1 (MQ=255)
                     gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACTCCCACAGGGTGAGGGGAcc                                                                                                                                    >  1:7888107/1‑150 (MQ=255)
                           tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                                                                              <  2:7160990/150‑1 (MQ=255)
                           tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCGCACCCACACGGGGGGGGGGCCCGtggg                                                                                                                               >  1:2319477/1‑147 (MQ=255)
                           tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACACCCGCAGGGAGCGGGGCCCGGTCgg                                                                                                                              >  1:11022597/1‑150 (MQ=255)
                            ggatgtgCGATATTACGCTTGATTCGCTGGTGCCGGGGCTGGGTTATGTGTTGCCTGCGGCACTGTCTGCCATGGCGAACGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGTGGGGACCGTTCGgc                                                                                                                             <  2:9697910/144‑1 (MQ=255)
                             gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCTGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                                                                            <  2:5011672/150‑1 (MQ=255)
                             gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAACCACACCCACAGGGAGAGGGGACCGGTCGgcg                                                                                                                            >  1:8103705/1‑150 (MQ=255)
                              aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCCCACCCACACCCACCGGGCGAGGGGCCCCGGCGgcgc                                                                                                                           >  2:6076583/1‑150 (MQ=255)
                               tCAGCGATATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                          <  2:5857977/150‑1 (MQ=255)
                               tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAACCACTCCCACACGGCGAGGGGACCCAGCGgcgcg                                                                                                                          >  1:11607880/1‑149 (MQ=255)
                                  gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCCCCAAAACCACAACCACAAGGAGAGGGGGCCGGGCGGCGCcgtt                                                                                                                       >  1:11402673/1‑149 (MQ=255)
                                   cGAAATTCCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGATTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                                                      <  1:10519520/150‑1 (MQ=255)
                                   cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                                                      <  1:3280182/150‑1 (MQ=255)
                                         tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTc                                                                                                                <  1:6888281/150‑1 (MQ=255)
                                            gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCCACCGAACCCAAACCCGCAGGGCGGGGGGACCCTTCGGCGCGGTATGTAATccca                                                                                                             >  1:7164681/1‑149 (MQ=255)
                                              aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCGCCCCCAAAACCAATCCCGACGGGAGAAGGGACCCTTAGGCGCAGTATGTAATCCCTcc                                                                                                           >  2:7925038/1‑149 (MQ=255)
                                              aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAACCCACAACCAAACGGGGAGGGGACCCATCGGCGCGGTATTTAcaaccaca                                                                                                           >  2:8449733/1‑143 (MQ=255)
                                              aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACACCCAAACGGGGAGGGGCCCGTTAGGCGCTGGAAGTACTcccccc                                                                                                           >  1:6756565/1‑147 (MQ=255)
                                              aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCCAAACCACAACCCAACGGCGCGGGGACCCGTAGGCGCGGGATGTACTCCcaca                                                                                                           >  2:11803888/1‑150 (MQ=255)
                                                gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGGCCCCGCACCCAAAACCTCTACCTCAGGGGGAGGGGACCGTTCGGCGCTGTTTGTACACCCGCAcg                                                                                                         >  2:12181280/1‑149 (MQ=255)
                                                    cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                    <  2:3771328/150‑1 (MQ=255)
                                                      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGTATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                  <  2:929964/150‑1 (MQ=255)
                                                      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                  <  1:5811077/150‑1 (MQ=255)
                                                      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                  <  1:3769569/150‑1 (MQ=255)
                                                         gTGCTGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGAATAGTCTTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGa                                                                                               <  1:5548830/150‑1 (MQ=255)
                                                           gctggggCTGGGTAATGTGTTGCGTGGGGCAGTGGCTCCCTTGGCGAATGGTATTGCGGCATAGTCGTTTGCGCCCCCTCACCATAACCCTCTCCCTCTGGGTGTGGGGACCGTTCGGGGCTGTATTTACTCCCTCACTCTTAAACGaca                                                                                             <  1:8304304/147‑1 (MQ=255)
                                                           gCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACTCCCACAGGGAGAGGGGCCCGTTCGGCGCAGGGAGTACTACCACACAATGAAACGacc                                                                                             >  1:11922402/1‑149 (MQ=255)
                                                             aGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACAcc                                                                                           <  2:3223697/150‑1 (MQ=255)
                                                             aGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAACCCACACCCACAGGGAGAGGGGGCCCGTAGGCGCGGTATTTACTGCCACACACTGGAACGccaac                                                                                           >  1:6451046/1‑148 (MQ=255)
                                                              ggggGTGGTTAATGTGTTGCCTGCGGCACTGGCGCCCATGGCGAATGGGATTGCGGATTAGTGGTTGGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGTGTGGGGACCGTTCGGCGCTGTTTTTACTCCCTCACTCTGAAACGACACCg                                                                                          <  1:10248115/150‑1 (MQ=255)
                                                                       tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                <  1:1122926/150‑1 (MQ=255)
                                                                       tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCAAAACCACTACCTCAGGGCGAGGGGGCCGGTGGGCGCAGTATGTTATCCCTCAAGATGGAAAGACACCCCCCACtttt                                                                                >  1:9551956/1‑150 (MQ=255)
                                                                        aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                               <  2:2463989/150‑1 (MQ=255)
                                                                           gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCTAACCCGCAACCACCGGGAGAGGGGACCGGTCGGCGCGGTATTTACTCCCACCCTCTGAAACGACACCCCCCTCttttttat                                                                            >  2:6789614/1‑148 (MQ=255)
                                                                           gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCAAACCCACTCCCACAGGGGGTGGGGCCCGGTCGGCGCTGGATGTTATCCCTCACACGGTAACGACACCCCACACttttttta                                                                            >  2:2888155/1‑149 (MQ=255)
                                                                              ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGTATAGTCGTGTGCGCCCCCCCCCCCAAACCCACTCCCGCAGGGCGGGGGGCCCCCTGGGCGCGGGTAGTACTCCCGCACGCTGAAACGCCACCGCCCTCTGTTTTTCTcc                                                                         >  2:8810020/1‑150 (MQ=255)
                                                                              ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtcgat                                                                         <  2:8011025/150‑4 (MQ=255)
                                                                              ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                         <  1:846208/150‑1 (MQ=255)
                                                                              ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCCCCACCAAAACCACACCCCCCGGGTCGGGGGGCCCGGCGGCGCGGTATGTAATCACCAAAACCTAAACGACACCCCACACATTTTTTGTcc                                                                         >  1:1798554/1‑150 (MQ=255)
                                                                                 ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                      <  1:7819487/150‑1 (MQ=255)
                                                                                 ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                      <  1:565808/150‑1 (MQ=255)
                                                                                 ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAACCCACACCCACACGGGGAGGGGGCCGGTGGGCGCGGTTTGTAAGACCACCCACAGAAACCACACCCCCCCCattttttatacccc                                                                      >  1:1539408/1‑143 (MQ=255)
                                                                                 ccTGCGGCACTGGCTCCCAGGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                      <  2:1692779/150‑1 (MQ=255)
                                                                                   tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCAGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                                    <  1:9919594/150‑1 (MQ=255)
                                                                                     cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                                  <  1:3895282/150‑1 (MQ=255)
                                                                                           tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                            <  2:10523359/150‑1 (MQ=255)
                                                                                           tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCACAACCACAGGGGGGGGGGGACGGTAGGCGCCGTGTGTACACCCCCACACTGTAAACACACCGCCCACTTTTTTTCTACCACGCCCCACCgg                                                            >  2:3939301/1‑150 (MQ=255)
                                                                                           gggCTCCCATGGCGAAAGTTATTGCGGCTTTGTCGTTGCCGCCCCCACACCCTACCCCCATCCCCCTGGTAGAGGGTACCTTTGGGCGCTGTTTGTCGCCCCACAAAAGGAAACGACACCGAACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                            <  1:10001155/149‑1 (MQ=255)
                                                                                            ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                           <  2:4191425/150‑1 (MQ=255)
                                                                                             gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAAACCACAACCACACGGTGAGGGGCCCCGTAGGCGCGGTATGTAAGACCACACACAGAAAACACACCCCACGCATTTTTTTTACCGCCCCCCACCgggg                                                          >  2:6372167/1‑150 (MQ=255)
                                                                                              cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                         <  2:4545379/150‑1 (MQ=255)
                                                                                               tCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCGAACCCCAAACCGCACACGCATGGGGAGGGGAACCTTACGCGCAGTATGTACTCCCCCAATCCGTAACGCCACCGCACACTTTTTTTCTCCCGCCCCCCACCcgggcg                                                        >  2:6939051/1‑148 (MQ=255)
                                                                                                 ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                       <  2:11241415/150‑1 (MQ=255)
                                                                                                   aTGGCGAATGGGATTGCGGCATAGTAGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                    <  1:7382368/150‑1 (MQ=255)
                                                                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCGCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCCGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                    <  2:9817960/150‑1 (MQ=255)
                                                                                                        gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCTCACCCACAGGGGGAGGGGACCCGTCGGCGCGGTGTGTACTCCCCCACGCAGGAAACACCCCGCCCAAGTTTTTTCTCCCACGCCCCACCCGGGGGAGGGCCgcg                                               >  2:6504219/1‑148 (MQ=255)
                                                                                                         aaaGGGAATGCGGAATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                                              <  1:3537036/150‑1 (MQ=255)
                                                                                                          aaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                             <  1:5075924/150‑1 (MQ=255)
                                                                                                            gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                           <  1:1298005/150‑1 (MQ=255)
                                                                                                            gggAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAAACCACAACCACAGGGCGCGGGGCACCGTCGGCGGGGTATGTACAACCCCACTCCGCAACCGACCCCCACGAGTTTTTTCTGCCTCGCCCCACCGGGGGGGGGGCCGGGGTgg                                           >  2:4777234/1‑149 (MQ=255)
                                                                                                            gggAATGCGGCATAGGCGTGTGCGCCCCCTCCCCCTACCCCTCGCCCTCAGGGAGAGGGGCCCGTTCGGCGCGGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCGCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                           <  1:312292/150‑1 (MQ=255)
                                                                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTTTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                          <  1:4314672/150‑1 (MQ=255)
                                                                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                          <  2:5299732/150‑1 (MQ=255)
                                                                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                          <  2:6649452/150‑1 (MQ=255)
                                                                                                              gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                         <  2:1369058/150‑1 (MQ=255)
                                                                                                              gAATGCGGCATAGTCGTGGGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTGCGGCGCTGTAGGGACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                         <  2:2298268/150‑1 (MQ=255)
                                                                                                               aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                        <  2:7782895/150‑1 (MQ=255)
                                                                                                                  gCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaa                                     <  2:11953082/150‑1 (MQ=255)
                                                                                                                    ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCACTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                   <  2:3467923/150‑1 (MQ=255)
                                                                                                                      cATAGTCGTTTGCGCCCCCTCACCCTCACCCTATCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACGGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGCGGGGACAAgg                                 <  2:958726/150‑1 (MQ=255)
                                                                                                                              tttGCGCCCCCTCACCCTAACCCAAACCCACAGGGAGAGGGGACCCTTCGGCGCAGTAAGTAATCCCTCACAATGAAACCACACCGCAAACATTTTTTAAACCACCCCCCACCGGGGAGAGGGCCCGGGTGAGGGGAAAAGGCCCCAcag                         >  2:2593186/1‑148 (MQ=255)
                                                                                                                                   gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                    >  2:5500941/1‑150 (MQ=255)
                                                                                                                                     ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCa                  >  2:1495385/1‑150 (MQ=255)
                                                                                                                                     ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAATCTTTTTTTCTCCCTCGCACCTCCTGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCa                  >  2:11542352/1‑150 (MQ=255)
                                                                                                                                       ccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAt                >  2:6212623/1‑150 (MQ=255)
                                                                                                                                           aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                                    <  2:10182988/94‑1 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCTGCTCACCAATTTTCTg          >  1:2596777/1‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg          >  1:10791560/1‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg          >  1:9286493/1‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg          >  2:11077250/1‑150 (MQ=255)
                                                                                                                                                tccccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa       >  2:7432385/3‑150 (MQ=255)
                                                                                                                                                aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa       >  1:2707456/1‑150 (MQ=255)
                                                                                                                                                aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa       >  2:1987974/1‑150 (MQ=255)
                                                                                                                                                 ccccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTAt      >  2:9069296/2‑150 (MQ=255)
                                                                                                                                                    ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt   >  1:7635235/1‑150 (MQ=255)
                                                                                                                                                     tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg  >  2:10733576/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
GTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTG  >  NC_000913/3780895‑3781193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: