Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,075 T→G 7.6% intergenic (+58/‑140) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0750TG 7.6% 2410.8 / 17.4 762intergenic (+58/‑140)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (284/378);  new base G (78/3);  total (381/381)
Fisher's exact test for biased strand distribution p-value = 6.42e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 847 total aligned reads displayed.

TCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACA  >  NC_000913/3780926‑3781224
                                                                                                                                                     |                                                                                                                                                     
tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGACTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                                                       <  1:6457240/150‑1 (MQ=255)
  aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAAACCACAACCTCAGGGAGAGGGGACCCGTCGGGGCggt                                                                                                                                                     >  1:11239132/1‑150 (MQ=255)
   gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCCCACCCAAAACCACAACCACACGGAGAGGGGGCCCGTCGGCGCagtt                                                                                                                                                    >  1:6281273/1‑149 (MQ=255)
    cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                                                                                   <  2:973981/150‑1 (MQ=255)
    cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                                                                                   <  1:8705034/150‑1 (MQ=255)
    cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                                                                                   <  1:3280182/150‑1 (MQ=255)
      aaaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGGGTGCCCCCCCCCCCCCCAACCCCCACCCCCCCGGCGCGGGGCCCCGTCGGCGCgggatgg                                                                                                                                                 >  2:11780471/1‑149 (MQ=255)
       aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                                                                                                <  1:11681139/150‑1 (MQ=255)
         ttACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAACCCACTCCCACAGGGAGAGGGGACCCGTCGGCGCTGGATGTACt                                                                                                                                              >  1:7684615/1‑150 (MQ=255)
          tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTc                                                                                                                                             <  1:6888281/150‑1 (MQ=255)
           aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                                                                            <  1:7183804/150‑1 (MQ=255)
             gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCACACCCACACGGTGAGGGGGCCCGTCGGCGCGGGATTTACTacca                                                                                                                                          >  1:5661614/1‑149 (MQ=255)
             gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAACCACTCCCGCAGGGGGAGGGGGCCGGTCGGCGCGGGATGTACTcccg                                                                                                                                          >  1:4082761/1‑149 (MQ=255)
             gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCCCCCAACCCCCACCCACAGGGGGAGGGGGCCGAGCGGGGCAGTGTGTACAcccc                                                                                                                                          >  1:11448686/1‑149 (MQ=255)
              cAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTACCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGAGCTGTATGTACTCCCTc                                                                                                                                         <  2:11293374/150‑1 (MQ=255)
                 gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATCTACTCCCTCAct                                                                                                                                      <  2:9254575/150‑1 (MQ=255)
                  aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                                                                                     <  1:3978476/150‑1 (MQ=255)
                    gcGCTGGTGCAGGGGCGGGGTCAAGTGTGGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTg                                                                                                                                   <  2:9363390/149‑1 (MQ=255)
                     cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                                                  <  2:2572693/150‑1 (MQ=255)
                      gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAAACCACTCCCACAGGGGGAGGGGGCCGGTAGGCGCTGTGTGTTCACCCACACACAGaa                                                                                                                                 >  1:670563/1‑150 (MQ=255)
                       cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                <  1:9711247/150‑1 (MQ=255)
                       cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                <  2:5382340/150‑1 (MQ=255)
                        tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                                                               <  2:10210704/150‑1 (MQ=255)
                          gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGa                                                                                                                             <  1:82441/150‑1 (MQ=255)
                               ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCGCCCCCTAAACCACACCCACAGGGAGGGGGGCCCCGTCGGCGCGGTATGTTCTCCCGCACTCTGCAACGACACCg                                                                                                                        >  2:6841761/1‑150 (MQ=255)
                               ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACCACCAAACCCACTCCCACCAGGGGAGGGGACCCGTCGGCGCGGTTTTTTAACCCTCACACGGAAAACACAccc                                                                                                                        >  2:8524753/1‑149 (MQ=255)
                                   cTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAct                                                                                                                    <  1:2601450/150‑1 (MQ=255)
                                    tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTTAAACGACACCGCActc                                                                                                                   <  1:11136907/150‑1 (MQ=255)
                                        tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACACCCACAGGGGGAGGGGCCCCGTCGGCGCGGTATGTAATACCACACACTGAAACGACACCCCCCACtttt                                                                                                               >  1:2830984/1‑150 (MQ=255)
                                         aaaGTGTTGGCTGCGGGACTGGCTCCCATGGCGAAAGGGTATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGTGGGGTCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                              <  2:12137944/150‑1 (MQ=255)
                                         aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                              <  2:1586215/150‑1 (MQ=255)
                                           agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                            <  1:10259931/150‑1 (MQ=255)
                                              gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                         <  1:1367205/150‑1 (MQ=255)
                                              gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                         <  2:11889490/150‑1 (MQ=255)
                                              gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                         <  1:2939147/150‑1 (MQ=255)
                                              gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAACGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                         <  2:1241098/150‑1 (MQ=255)
                                               ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                        <  2:1789056/150‑1 (MQ=255)
                                               ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCCAAACCACACCCACAGGGCGGGGGGGACCTGCCGCGCGGTGTGTACACCCCCACGCAGGAACGACACCCCACACTTGTTTTCTcc                                                                                                        >  2:10979140/1‑150 (MQ=255)
                                               gtGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                        <  1:7855281/149‑1 (MQ=255)
                                                tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTccc                                                                                                       <  2:5688818/150‑1 (MQ=255)
                                                  ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                                                     <  1:3818848/150‑1 (MQ=255)
                                                  ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                                                     <  2:1478976/150‑1 (MQ=255)
                                                  ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAACCCACACCCACACGGGGAGGGGGCCGGTGGGCGCGGTTTGTAAGACCACCCACAGAAACCACACCCCCCCCattttttatacccc                                                                                                     >  1:1539408/1‑143 (MQ=255)
                                                    tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                                                                   <  1:2168559/150‑1 (MQ=255)
                                                    tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                                                                   <  2:9045768/150‑1 (MQ=255)
                                                            tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                           <  2:10523359/150‑1 (MQ=255)
                                                            tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                           <  1:1184196/150‑1 (MQ=255)
                                                            gggCTCCCATGGCGAAAGTTATTGCGGCTTTGTCGTTGCCGCCCCCACACCCTACCCCCATCCCCCTGGTAGAGGGTACCTTTGGGCGCTGTTTGTCGCCCCACAAAAGGAAACGACACCGAACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                           <  1:10001155/149‑1 (MQ=255)
                                                             ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTTAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                          <  1:322560/150‑1 (MQ=255)
                                                             ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                          <  1:5547411/150‑1 (MQ=255)
                                                             ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                          <  2:5133201/150‑1 (MQ=255)
                                                             ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                          <  2:5931187/150‑1 (MQ=255)
                                                             ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                          <  2:4191425/150‑1 (MQ=255)
                                                             ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCCCCCCCCCCCCCAAACCCAAACCCCAACGGCAAGGGGGCCCGTCGGCCCGGGAAGTACACCCACCCCCGGCAACGCCACCGCCCGGTTTTTTTAACCCGCCCCCCACCggg                                                                                          >  2:10788109/1‑150 (MQ=255)
                                                               cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                        <  1:60931/150‑1 (MQ=255)
                                                               cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCGCTCCCACAGGGAGAGGGGCCCGGTCGGCGCTGGATGTCCTCCCGCACTCTGAAACGACACCCCCCGCTTTTTTTCTCCCTCGCCCCGCCGGGga                                                                                        >  2:7974823/1‑150 (MQ=255)
                                                               cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAACCCACACCCACAGGGGGAGGGGACCCTTCGGCGCGGTTTGTACTCCCACACACGGAAACGCCACCCCACTCTTTTTTTCTCCCTCGCCCCCCCggggg                                                                                        >  2:11117621/1‑149 (MQ=255)
                                                                 cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                      <  1:1729207/150‑1 (MQ=255)
                                                                  ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                     <  2:7392082/150‑1 (MQ=255)
                                                                  ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                     <  1:10525886/150‑1 (MQ=255)
                                                                   cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgg                                                                                    <  1:1897130/150‑1 (MQ=255)
                                                                   cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACTACCGCAGGGCGGGGGGGCCGGTCGGGGCGGGAAGTACTCCCACACACGGGAACGACCCCCCCCACTTTTTTTATACCACGCCCCACCcgggggcgg                                                                                    >  2:7554756/1‑145 (MQ=255)
                                                                    aTGGCGAATGGGATTGCGGCATAGTAGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                   <  1:7382368/150‑1 (MQ=255)
                                                                    aTGGCGAAAGGGAAGGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                   <  2:4656761/150‑1 (MQ=255)
                                                                         gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCTAACCCACACCCACAGGGAGTGGGGACCCTTGGGCGCTGTTTGTACTCCCTCACACTGAAACGACACCCCACTATTTTTTTATCCCTAGCCCCTCCGGGGAGGGGGCCggg                                                                              >  2:7795829/1‑150 (MQ=255)
                                                                             gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                          <  1:10904535/150‑1 (MQ=255)
                                                                             gggAATGCGGCATAGTCGAGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                          <  2:6705342/150‑1 (MQ=255)
                                                                              ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                         <  2:440372/150‑1 (MQ=255)
                                                                               gAATGCGGCATAGTCGTGGGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTGCGGCGCTGTAGGGACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                        <  2:2298268/150‑1 (MQ=255)
                                                                                aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                       <  2:3964399/150‑1 (MQ=255)
                                                                                     ggCATAGTCGTTTGCGCCCCCTCACCCTACCCCACTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                  <  2:4898772/150‑1 (MQ=255)
                                                                                     ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                  <  2:6433544/150‑1 (MQ=255)
                                                                                              gTTTGCGCCCCCTGACCCTAAACCTATACAGCAGGGAGAGGGGAAAGTTAGGCCCTTTATGTACTACCTAACACTGAAACGAAAACGCAATCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGGGGGGAAAATGCCGCACt                                                         >  2:3297517/1‑150 (MQ=255)
                                                                                              gTTTGCGCCCCCACACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCATTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAACGGCCGCACt                                                         >  2:3751812/1‑150 (MQ=255)
                                                                                               tttGCGCCCCCTCACCCTAAACCTCTCCCTCAGGGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACGTCACCGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTg                                                        >  2:6325065/1‑150 (MQ=255)
                                                                                               tttGCGCCCCCACACCCTAAACCACACCCACAGGGAGAGGGGACCGTTAGGCGCAGTATGTAATACCGCACACTGAAACGACACCCCCCAAATTTTTTATACCACGCCCCACCCGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCAcag                                                        >  1:8471113/1‑148 (MQ=255)
                                                                                                    gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                   >  1:3544070/1‑150 (MQ=255)
                                                                                                      ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAATCTTTTTTTCTCCCTCGCACCTCCTGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCa                                                 >  2:11542352/1‑150 (MQ=255)
                                                                                                         cTCCCCCTAGCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAACGGCCGCACTGCTCACCAAtt                                              >  2:8768223/1‑150 (MQ=255)
                                                                                                         cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                              >  2:6182979/1‑150 (MQ=255)
                                                                                                              ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                         >  2:9649819/1‑150 (MQ=255)
                                                                                                                tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                       >  1:4668842/1‑150 (MQ=255)
                                                                                                                tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                       >  1:9457539/1‑150 (MQ=255)
                                                                                                                    ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                   >  2:2712443/1‑150 (MQ=255)
                                                                                                                      tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                 >  2:8341262/1‑150 (MQ=255)
                                                                                                                      tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                 >  1:12122493/1‑150 (MQ=255)
                                                                                                                          ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                             >  2:6524616/1‑150 (MQ=255)
                                                                                                                          ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                             >  2:3124956/1‑150 (MQ=255)
                                                                                                                                    agGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATGTTCTGCTATCCTGCCCCCGCACTAAgg                   >  2:9903405/1‑150 (MQ=255)
                                                                                                                                     ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                  >  2:7006940/1‑150 (MQ=255)
                                                                                                                                      gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                 >  2:8085467/1‑150 (MQ=255)
                                                                                                                                           cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt            >  2:2428224/1‑150 (MQ=255)
                                                                                                                                           cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTACTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt            >  1:5251874/1‑150 (MQ=255)
                                                                                                                                             ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt          >  2:2040328/1‑150 (MQ=255)
                                                                                                                                             ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt          >  1:8900083/1‑150 (MQ=255)
                                                                                                                                                 gcgcTGTATGTACTCCCGCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCGCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCGCACCAATATGCGGCTATCCTGCCCCCGCACTAGGGGGGCAGTCTGCTa      >  1:5795336/1‑150 (MQ=255)
                                                                                                                                                  cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa     >  2:2909069/1‑150 (MQ=255)
                                                                                                                                                  cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa     >  1:4988417/1‑150 (MQ=255)
                                                                                                                                                   gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCGCACTAAGGGGGCAGTATGCTAAAc   >  1:7954045/1‑150 (MQ=255)
                                                                                                                                                     tgtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa  >  1:9117217/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACA  >  NC_000913/3780926‑3781224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: