Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,092 T→A 6.5% intergenic (+75/‑123) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0920TA 6.5% 2649.1 / 34.0 805intergenic (+75/‑123)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (380/335);  new base A (69/2);  total (468/337)
Fisher's exact test for biased strand distribution p-value = 1.32e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 831 total aligned reads displayed.

GATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCA  >  NC_000913/3780943‑3781241
                                                                                                                                                      |                                                                                                                                                       
    cGCTGGTGCAGGGGCTGGGTAACGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCCTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                                                                    <  1:3493000/150‑1 (MQ=255)
    cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                                                                    <  2:2572693/150‑1 (MQ=255)
    cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                                                                    <  2:966746/150‑1 (MQ=255)
    cGCTGGTGCAGGGGCTGGGTAAAGAGGTGCCTGCGGCACTGGCTCCCAGGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                                                                    <  2:5048592/150‑1 (MQ=255)
      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                                  <  2:8135097/150‑1 (MQ=255)
         gTGCTGGGGGTGGGTAAAGAGTTGCCTGCGGCACGGGCTCCCATGGCGAAAGGGAATGCGGCTTTGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGa                                                                                                                                               <  1:5312374/150‑1 (MQ=255)
          tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGac                                                                                                                                              <  2:9046114/150‑1 (MQ=255)
          tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCAAACCCACACCCCCACGGAGGGGGGACCGTGCCGCGCGGGATGTACACACGCACACTGaaaacac                                                                                                                                              >  2:5826961/1‑146 (MQ=255)
                ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAATGGGAATGCGGCTTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                                                        <  1:7666777/150‑1 (MQ=255)
                  cTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAAACCACTCCCACAAGGGGGGGGGGCCGTTGGGCGCGGTATGTACACCCACAAACAGAAACCACACCCCaaa                                                                                                                                      >  2:1329984/1‑148 (MQ=255)
                     ggTAATGTGTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTTTGTACTCCCTCACTCTGAAACGACACCGCACTCtt                                                                                                                                   <  1:4413942/150‑1 (MQ=255)
                       tAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                                 <  1:1123203/150‑1 (MQ=255)
                       tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCAAAACCACTACCTCAGGGCGAGGGGGCCGGTGGGCGCAGTATGTTATCCCTCAAGATGGAAAGACACCCCCCACtttt                                                                                                                                 >  1:9551956/1‑150 (MQ=255)
                        aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAACGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                                                <  1:8259385/150‑1 (MQ=255)
                          agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCGCACCCAAACCCAAAACCACCCGGGGGGGGGCCCCTTAGGCGCGGTTTGGACTACCACACACTGAAAACACACCCCCCACatttttt                                                                                                                              >  1:8727230/1‑150 (MQ=255)
                          agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                                              <  1:10259931/150‑1 (MQ=255)
                           gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTCACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                                            <  2:11148673/150‑1 (MQ=255)
                            agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACAACCTAACCCACTCCCTCAGGGAGAGAGGACCGTTCGGCGCTGTATTTTCTCCCACACACTGAAAAGACACCCCACACgttttttat                                                                                                                            >  2:1481341/1‑148 (MQ=255)
                             gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                                          <  1:2939147/150‑1 (MQ=255)
                             gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACACTTTTTTtctc                                                                                                                          <  2:1060474/150‑1 (MQ=255)
                              ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtcgat                                                                                                                         <  2:40882/150‑4 (MQ=255)
                              gtGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                         <  1:7855281/149‑1 (MQ=255)
                              gtGCCTGCGGCACTGGCGCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                         <  2:7985538/149‑1 (MQ=255)
                                gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCt                                                                                                                       <  1:10689822/150‑1 (MQ=255)
                                   tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                                                                                    <  1:11853231/150‑1 (MQ=255)
                                    gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                                                                   <  2:1162394/150‑1 (MQ=255)
                                    gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTGGCGCCCCCGCCCCCGGAACCGCGCCCGCAGGGGGAGGGGACCCTTCGGCGCGGTATGTGCAGCCGCGCGCAGAAACGCCACCCCCCGCGTTTTTTCTCCCGCGcc                                                                                                                   >  2:6532161/1‑150 (MQ=255)
                                          cTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCAAACCCACAACCACAGGGCGTGGGGTCCCTTACGCGCGGTGTGTAATCCCACACACTGGAACCACACCCCACACTTTTTTTGTCCCGCGCCCCTCCg                                                                                                             >  2:1632239/1‑150 (MQ=255)
                                           tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCACAACCACAGGGGGGGGGGGACGGTAGGCGCCGTGTGTACACCCCCACACTGTAAACACACCGCCCACTTTTTTTCTACCACGCCCCACCgg                                                                                                            >  2:3939301/1‑150 (MQ=255)
                                           gggCTCCCATGGCGAAAGTTATTGCGGCTTTGTCGTTGCCGCCCCCACACCCTACCCCCATCCCCCTGGTAGAGGGTACCTTTGGGCGCTGTTTGTCGCCCCACAAAAGGAAACGACACCGAACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                            <  1:10001155/149‑1 (MQ=255)
                                            ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                           <  2:2454211/150‑1 (MQ=255)
                                            ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                           <  1:4995914/150‑1 (MQ=255)
                                             gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAAACCACAACCACACGGTGAGGGGCCCCGTAGGCGCGGTATGTAAGACCACACACAGAAAACACACCCCACGCATTTTTTTTACCGCCCCCCACCgggg                                                                                                          >  2:6372167/1‑150 (MQ=255)
                                              cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                         <  2:4545379/150‑1 (MQ=255)
                                                cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                       <  1:1729498/150‑1 (MQ=255)
                                                 ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                      <  1:11562087/150‑1 (MQ=255)
                                                  cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgg                                                                                                     <  1:1897130/150‑1 (MQ=255)
                                                  cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                     <  1:6163731/150‑1 (MQ=255)
                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTTGGCCCCCGCACCCAAACACAAAACCAAATGGTGTGGGGTCCGGTAGGGGCGGTATGTACTCCCGCAAACTTAAAAGACACCCCACACATTTTTTTTCCCTCGCCCCACCCGGGCGCggg                                                                                                    >  2:6455518/1‑150 (MQ=255)
                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCGCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCCGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                    <  2:9817960/150‑1 (MQ=255)
                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                    <  1:2734638/150‑1 (MQ=255)
                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                    <  2:6226111/150‑1 (MQ=255)
                                                    tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCGCTCCCGCAGGGGGTGGGGGCCCGTGGGCGCGGTTTGTTCTCCCACACACAGGAACCGCCCCCCCCAATTTTTTTATCCCACGCCCCACCGGGGGGGGGGc                                                                                                   >  2:6067146/1‑150 (MQ=255)
                                                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                <  1:2401249/150‑1 (MQ=255)
                                                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                <  1:5562128/150‑1 (MQ=255)
                                                            gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                           <  1:10904535/150‑1 (MQ=255)
                                                            gggAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAAACCACAACCACAGGGCGCGGGGCACCGTCGGCGGGGTATGTACAACCCCACTCCGCAACCGACCCCCACGAGTTTTTTCTGCCTCGCCCCACCGGGGGGGGGGCCGGGGTgg                                                                                           >  2:4777234/1‑149 (MQ=255)
                                                            gggAATGCGGCATAGGCGTGTGCGCCCCCTCCCCCTACCCCTCGCCCTCAGGGAGAGGGGCCCGTTCGGCGCGGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCGCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                           <  1:312292/150‑1 (MQ=255)
                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                          <  2:5299732/150‑1 (MQ=255)
                                                              gAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTCTGTACTCCCTCACTCGGAACCGACACCGCACTCTGTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                         <  2:8851782/150‑1 (MQ=255)
                                                              gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                         <  1:7214365/150‑1 (MQ=255)
                                                              gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                         <  1:633373/150‑1 (MQ=255)
                                                              gAATGCGGCATAGTCGTGGGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTGCGGCGCTGTAGGGACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                         <  2:2298268/150‑1 (MQ=255)
                                                                    ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                   <  2:7455034/150‑1 (MQ=255)
                                                                    ggCATAGTCGTTTGCGCCCCCCCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                   <  2:2144894/150‑1 (MQ=255)
                                                                     gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg                                                                                  <  2:2904548/150‑1 (MQ=255)
                                                                      cATAGTCGTTTGCGCCCCCTCACCCTCACCCTATCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACGGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGCGGGGACAAgg                                                                                 <  2:958726/150‑1 (MQ=255)
                                                                           tCGTTTGCGCCCCCTCACCCAAACCCGCACCCTCAGGGAGAGGGGACCCTTCGGCGCCGTAAGTAATCCCTCACTCTGAAACGACACCCCACTCTTTTTTTATACCTCGCCCCACCCGGGAGAGGGCCGGGGTGAGGGGCAAAGGCCCCa                                                                            >  2:3743342/1‑150 (MQ=255)
                                                                              tttGCGCCCCCACACCCTAAACCACACCCACAGGGAGAGGGGACCGTTAGGCGCAGTATGTAATACCGCACACTGAAACGACACCCCCCAAATTTTTTATACCACGCCCCACCCGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCAcag                                                                         >  1:8471113/1‑148 (MQ=255)
                                                                                   gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                    >  2:5500941/1‑150 (MQ=255)
                                                                                      cccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCaa                                                                 >  2:11918839/1‑150 (MQ=255)
                                                                                           aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCCTCCCACGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGCCCGCACTGCGCACCAAtttt                                                             >  2:9279430/1‑150 (MQ=255)
                                                                                              cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                         >  1:6540498/1‑150 (MQ=255)
                                                                                               tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                        >  2:4309896/1‑150 (MQ=255)
                                                                                                tccccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAACAGGCCGCACTGCTCACCAATTTTCTGCTa                                                       >  2:11787708/3‑150 (MQ=255)
                                                                                                  cccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCATATTTCTGCTATc                                                     >  2:6858494/1‑150 (MQ=255)
                                                                                                   ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                    >  2:2712443/1‑150 (MQ=255)
                                                                                                    ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCCATTTTCTGCTATCCt                                                   >  2:8341566/1‑150 (MQ=255)
                                                                                                    ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                   >  2:10334270/1‑150 (MQ=255)
                                                                                                         ccTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                              >  2:1836392/1‑150 (MQ=255)
                                                                                                         ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                              >  1:5739099/1‑150 (MQ=255)
                                                                                                                 agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                      >  1:1509472/1‑150 (MQ=255)
                                                                                                                    ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                   >  2:7006940/1‑150 (MQ=255)
                                                                                                                     gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                  >  2:8652590/1‑150 (MQ=255)
                                                                                                                     gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACAGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                  >  1:3279826/1‑150 (MQ=255)
                                                                                                                       gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                >  2:3035247/1‑150 (MQ=255)
                                                                                                                       gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                >  1:9082376/1‑150 (MQ=255)
                                                                                                                         aCCGTTCGGCGCTGTATGTACTCACTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAACAGGCCGCACTGCTCACCAATATTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                               >  2:7691164/1‑150 (MQ=255)
                                                                                                                           cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                             >  2:3293867/1‑150 (MQ=255)
                                                                                                                           cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                             >  2:11355592/1‑150 (MQ=255)
                                                                                                                                ggggcTGTTTGTACACCCCCCCTCTCAACCGCCCCCGATTTCTTTTTTCCGCCCGCCCCCCTGGGGGGTGTGGGGGGGGGTGTGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                        <  2:7168462/147‑1 (MQ=255)
                                                                                                                                  cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                      >  1:1219280/1‑150 (MQ=255)
                                                                                                                                  cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGACCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                      >  1:4792765/1‑150 (MQ=255)
                                                                                                                                   gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                     >  1:4018867/1‑150 (MQ=255)
                                                                                                                                   gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCGCACTAAGGGGGCAGTATGCTAAAc                    >  1:7954045/1‑150 (MQ=255)
                                                                                                                                       tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                 >  2:12151668/1‑150 (MQ=255)
                                                                                                                                       tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                 >  1:2153217/1‑150 (MQ=255)
                                                                                                                                        atgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                >  2:6734296/1‑150 (MQ=255)
                                                                                                                                          gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa              >  2:10808631/1‑150 (MQ=255)
                                                                                                                                          gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa              >  2:7903935/1‑150 (MQ=255)
                                                                                                                                              tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt          >  1:11819062/1‑150 (MQ=255)
                                                                                                                                                ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc        >  1:11144458/1‑150 (MQ=255)
                                                                                                                                                ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc        >  1:9324530/1‑150 (MQ=255)
                                                                                                                                                ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc        >  1:6789196/1‑150 (MQ=255)
                                                                                                                                                 cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg       >  1:3855945/1‑150 (MQ=255)
                                                                                                                                                 cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg       >  1:5896047/1‑150 (MQ=255)
                                                                                                                                                 cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg       >  2:11008610/1‑150 (MQ=255)
                                                                                                                                                   cACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGaa     >  1:7390431/1‑150 (MQ=255)
                                                                                                                                                      tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa  >  1:3748522/1‑150 (MQ=255)
                                                                                                                                                      tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa  >  1:9880983/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCA  >  NC_000913/3780943‑3781241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: