Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,158 A→C 5.3% intergenic (+141/‑57) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1580AC 5.3% 2661.2 / 55.2 845intergenic (+141/‑57)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (764/19);  new base C (24/37);  total (788/57)
Fisher's exact test for biased strand distribution p-value = 2.34e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 874 total aligned reads displayed.

GCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATC  >  NC_000913/3781009‑3781307
                                                                                                                                                        |                                                                                                                                                     
  ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGaaaa                                                                                                                                                     <  2:6433544/150‑1 (MQ=255)
   gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAg                                                                                                                                                    <  2:2904548/150‑1 (MQ=255)
         tCGTTTGCGCCCCCTCACCCGAACCCACACCATCAGGGAGAGGGGACCCTTCGGCGCTGTATGTACACCCTCACTCTGAACCGCCACCGCACACTTTTTTTCTCCCTCGCCCCACCGGGG‑‑AGAGGGCCGGGGTGAGGGGCCAAGGCCGCa                                                                                                                                              >  2:11599017/1‑150 (MQ=255)
            tttGCGCCCCCTCACCCTAACCCAAACCCACAGGGAGAGGGGACCCTTCGGCGCAGTAAGTAATCCCTCACAATGAAACCACACCGCAAACATTTTTTAAACCACCCCCCACCGGGG‑‑AGAGGGCCCGGGTGAGGGGAAAAGGCCCCAcag                                                                                                                                           >  2:2593186/1‑148 (MQ=255)
            tttGCGCCCCCACACCCTAAACCACACCCACAGGGAGAGGGGACCGTTAGGCGCAGTATGTAATACCGCACACTGAAACGACACCCCCCAAATTTTTTATACCACGCCCCACCCGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCAcag                                                                                                                                           >  1:8471113/1‑148 (MQ=255)
                cgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCCCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCa                                                                                                                                       <  2:10444810/150‑1 (MQ=255)
                      cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGC‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                                                                                                                 <  1:2656354/150‑1 (MQ=255)
                            cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                                                                           >  1:6540498/1‑150 (MQ=255)
                             tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                                                                          >  1:5601448/1‑150 (MQ=255)
                                 ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                      >  2:2712443/1‑150 (MQ=255)
                                  ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                                                     >  2:1087803/1‑150 (MQ=255)
                                   tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                                                    >  2:5097458/1‑150 (MQ=255)
                                     tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                                                                                                  >  2:4077592/1‑150 (MQ=255)
                                         tgaGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                                                                              >  1:2835445/3‑150 (MQ=255)
                                         tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                                                                              >  1:9496189/1‑150 (MQ=255)
                                           aGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCa                                                                                                            >  2:1647110/1‑150 (MQ=255)
                                              gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                                                                         >  1:1618753/1‑150 (MQ=255)
                                                   gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                                                    >  2:9255361/1‑150 (MQ=255)
                                                   gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                                                    >  2:1177143/1‑150 (MQ=255)
                                                   gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACAGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                                                    >  1:3279826/1‑150 (MQ=255)
                                                    ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                                                   >  2:1209777/1‑150 (MQ=255)
                                                     gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                                                                                  >  1:9082376/1‑150 (MQ=255)
                                                        cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                                               >  2:4814043/1‑150 (MQ=255)
                                                        cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                                               >  1:5251815/1‑150 (MQ=255)
                                                        cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                                               >  2:2428224/1‑150 (MQ=255)
                                                               cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                                                                        >  1:4988417/1‑150 (MQ=255)
                                                                  tgtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTATCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa                                                                                     >  2:7697641/1‑150 (MQ=255)
                                                                   gtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCAGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt                                                                                    >  2:7567690/1‑150 (MQ=255)
                                                                       gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                                                                                >  1:4756513/1‑150 (MQ=255)
                                                                           tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                                                                            >  2:5966827/1‑150 (MQ=255)
                                                                           tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                                                                            >  1:9292667/1‑150 (MQ=255)
                                                                           tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                                                                            >  2:7366861/1‑150 (MQ=255)
                                                                           tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGAGGGCAGTATGCTAAACATCGTACttt                                                                            >  1:10782807/1‑150 (MQ=255)
                                                                            cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                                                                           >  2:7159354/1‑150 (MQ=255)
                                                                             ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                                                          >  1:11144458/1‑150 (MQ=255)
                                                                             ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTGTAc                                                                          >  1:1051465/1‑150 (MQ=255)
                                                                               tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                                                        >  2:6363524/1‑150 (MQ=255)
                                                                                cACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGaa                                                                       >  1:7390431/1‑150 (MQ=255)
                                                                                    ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                                                   >  1:722111/1‑150 (MQ=255)
                                                                                     tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa                                                                  >  1:11915067/1‑150 (MQ=255)
                                                                                            acacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCg                                                           >  1:229551/1‑150 (MQ=255)
                                                                                               caccccactTTTTTTTTCCCCCGCCCCCCCGGGGGTCGGGGGCGGGGGTGGGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc                                                          <  2:8011152/143‑1 (MQ=255)
                                                                                                cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  1:11340512/1‑150 (MQ=255)
                                                                                                cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  2:8755145/1‑150 (MQ=255)
                                                                                                cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                                                       >  1:1361789/1‑150 (MQ=255)
                                                                                                 gCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGGGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaaa                                                      >  1:1208135/1‑150 (MQ=255)
                                                                                                     tctTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                                                  >  1:7913221/1‑150 (MQ=255)
                                                                                                     tctTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                                                  >  2:2250389/1‑150 (MQ=255)
                                                                                                     tctTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                                                  >  2:3252092/1‑150 (MQ=255)
                                                                                                     tctTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                                                  >  2:3005574/1‑150 (MQ=255)
                                                                                                     tctTTTTTTCCCCCGCGCCCCGGGGGGT‑‑TGTGGGGGGGGGTGTGGGGCCAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                                                  <  2:11202343/150‑1 (MQ=255)
                                                                                                     ctcttttttCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                                                  >  1:3702610/4‑150 (MQ=255)
                                                                                                      atttttttCTCCCTCGCCCGTGGGGGG‑‑TGCGGGCGGGGGTGTGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                                                 <  2:12273429/149‑1 (MQ=255)
                                                                                                       tttttttCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGTTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  1:5992406/1‑150 (MQ=255)
                                                                                                       tttttttCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAGCAGAAATTCCGCCAAATACTgg                                                >  2:3683774/1‑150 (MQ=255)
                                                                                                       tttttttCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  1:3850192/1‑150 (MQ=255)
                                                                                                       tttttttCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                >  2:6928890/1‑150 (MQ=255)
                                                                                                       tttttttCACCCCCGCCCCGGGGGGT‑‑TGTGGGCGGGGGTGTGGGCAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                <  2:8989859/150‑1 (MQ=255)
                                                                                                        ttttttCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                                               >  2:11028922/1‑150 (MQ=255)
                                                                                                           tttCTCCCTCGCCCCTCGGGGG‑‑TGAGGTCCGGGGTGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAc                                            <  1:8170566/150‑1 (MQ=255)
                                                                                                           tttCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAc                                            >  2:5419911/1‑150 (MQ=255)
                                                                                                           gtgccccccCGCCCCGCGGGGG‑‑TGGGGGCGGGGGTGGGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAc                                            <  2:8229067/145‑1 (MQ=255)
                                                                                                            ccggcccTCTCCCCGGAGGGG‑‑CGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATGTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAca                                           >  2:10708874/5‑150 (MQ=255)
                                                                                                              ctcCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGACCCAGAAATTCCGCCAAATACTGGCAAcatc                                         >  1:12230633/1‑150 (MQ=255)
                                                                                                              ctcCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGACATTCCGCCAAATACGGGCAAcatc                                         >  1:7564344/1‑150 (MQ=255)
                                                                                                                cccTCACCCCGGAGGGG‑‑CGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat                                       >  1:5456472/1‑150 (MQ=255)
                                                                                                                 ccTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc                                      >  1:8746268/1‑150 (MQ=255)
                                                                                                                  cTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                                     >  2:8423117/1‑150 (MQ=255)
                                                                                                                  cTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                                     >  1:4632839/1‑150 (MQ=255)
                                                                                                                  cTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc                                     >  2:3864723/1‑150 (MQ=255)
                                                                                                                  cTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATATTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACGGGCAACATCATcc                                     >  1:10885097/1‑150 (MQ=255)
                                                                                                                   tCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                                    >  1:2116832/1‑150 (MQ=255)
                                                                                                                   tCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                                    >  1:4363119/1‑150 (MQ=255)
                                                                                                                   tCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                                    >  1:6761832/1‑150 (MQ=255)
                                                                                                                   tCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                                    >  2:2099716/1‑150 (MQ=255)
                                                                                                                   tCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                                    >  1:2428993/1‑150 (MQ=255)
                                                                                                                   tCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTATACGAACCAGAAAATCCGCCAAATACTGTCAACATCATCCg                                    >  1:3499528/1‑150 (MQ=255)
                                                                                                                       cccTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt                                >  1:11689138/1‑150 (MQ=255)
                                                                                                                        gcggggggg‑‑ggTGGGTCGGTGTGCGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTTCGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt                               <  1:6183954/145‑1 (MQ=255)
                                                                                                                         cTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTg                              >  2:10112780/1‑150 (MQ=255)
                                                                                                                         cTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCACCAAATACTGGCAACATCATCCGTCTTTg                              >  1:5811132/1‑150 (MQ=255)
                                                                                                                          tCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc                             >  1:3051748/1‑150 (MQ=255)
                                                                                                                                   cgagGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATa                      >  1:9784248/2‑150 (MQ=255)
                                                                                                                                     agGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATAcc                    >  2:4301941/1‑150 (MQ=255)
                                                                                                                                       ggCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCgg                  >  2:9692269/1‑150 (MQ=255)
                                                                                                                                       ggCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCgg                  >  2:5486695/1‑150 (MQ=255)
                                                                                                                                       ggCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCgg                  >  2:1408499/1‑150 (MQ=255)
                                                                                                                                       ggCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCgg                  >  1:7731540/1‑150 (MQ=255)
                                                                                                                                        gCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGc                 >  1:1017313/1‑150 (MQ=255)
                                                                                                                                        gCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGc                 >  1:10017398/1‑150 (MQ=255)
                                                                                                                                         ccGGGGTGCGGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGc                 <  2:4463074/150‑1 (MQ=255)
                                                                                                                                              gTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGt           >  2:1840174/1‑150 (MQ=255)
                                                                                                                                                   gggACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCa      <  2:6172573/150‑1 (MQ=255)
                                                                                                                                                   gggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCa      >  1:6389895/1‑150 (MQ=255)
                                                                                                                                                   gggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCa      >  1:1840685/1‑150 (MQ=255)
                                                                                                                                                   gggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCa      >  2:9602450/1‑150 (MQ=255)
                                                                                                                                                    ggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCat     >  2:669079/1‑150 (MQ=255)
                                                                                                                                                    ggAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCat     >  1:4118781/1‑150 (MQ=255)
                                                                                                                                                     gAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACGAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCata    >  2:11949719/1‑150 (MQ=255)
                                                                                                                                                     gAAAAGGACGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCata    >  1:7034179/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
GCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGG‑‑AGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATC  >  NC_000913/3781009‑3781307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: