BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,828,980	A→C	7.0%	Q12H (CAA→CAC)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,980	0	A	C	7.0%	3113.8 / 43.3	1194	Q12H (CAA→CAC)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (598/479); new base C (22/93); total (620/574)
Fisher's exact test for biased strand distribution p-value = 4.98e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1240 total aligned reads displayed.

ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  NC_000913/3828831‑3829128

 tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTCCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAc                                                                                                                                                     >  2:5397140/1‑150 (MQ=255)

 tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                     <  2:9190087/150‑1 (MQ=255)

 tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTATTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGAATGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                     >  2:12222324/1‑150 (MQ=255)

  ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  1:7675135/1‑150 (MQ=255)

   taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                   <  2:5226304/150‑1 (MQ=255)

    aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                  >  2:9387021/1‑150 (MQ=255)

     aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  1:10007209/150‑1 (MQ=255)

     aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 >  1:7462247/1‑150 (MQ=255)

             cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                                                                                                                         <  2:4133688/150‑1 (MQ=255)

              aTTTATCTCATTTAAATAGTTAGAAGAGCAAGTGGCAAACGTTAGCTATTCACATACGGTCAGCTAAAATGCCCCCTTTCCTACCACGGGATTGATATCGATGTCTGTATCCACCCTCGAGTCAGAAAATGCGCAACCGGATGCGCAGAc                                                                                                                                        >  2:1637187/1‑150 (MQ=255)

                ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCattttc                                                                                                                                      <  2:7618002/150‑6 (MQ=255)

                  aTCTAATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTAAGAAAATGCGCAACCGGTTGCGCAGACTCaa                                                                                                                                    <  2:2422434/150‑1 (MQ=255)

                    ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                  >  2:5699065/1‑150 (MQ=255)

                     tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                                 >  2:2851335/1‑150 (MQ=255)

                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                               >  1:6083611/1‑150 (MQ=255)

                        tttAAATTTTTAGATGAGGTTGTGGCAAAGGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCTCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                                                                                                              <  2:8851730/150‑1 (MQ=255)

                        tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                                                                                                              >  1:3656859/1‑150 (MQ=255)

                        tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTGTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                                                                                                              <  2:11040893/150‑1 (MQ=255)

                           aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                           >  1:7123812/1‑150 (MQ=255)

                             atttttAGCTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCTGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACt                                                                                                                         <  2:11420153/147‑1 (MQ=255)

                                    gATGTGTATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCGAGTAATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  <  2:2087443/150‑1 (MQ=255)

                                    gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  >  1:4798189/1‑150 (MQ=255)

                                             gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                         <  2:5084007/150‑1 (MQ=255)

                                             gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                         <  2:1358085/150‑1 (MQ=255)

                                             gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGATTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                         <  2:4728253/150‑1 (MQ=255)

                                                 cAACCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCTCGGGATTGTTTTCGATGTCTTTTTCCCCCCTCGATTCATAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                     <  1:5050975/150‑1 (MQ=255)

                                                 cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                     <  2:7819960/150‑1 (MQ=255)

                                                  aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACGCAAAAAAGCGAACTGATTTACCGTCTTTAAGAtag                                                                                                    >  1:11573036/1‑148 (MQ=255)

                                                     cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                 <  2:5870328/150‑1 (MQ=255)

                                                        ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTTAAGATCGTccgcc                                                                                              >  1:11232593/1‑150 (MQ=255)

                                                           cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                           >  2:8554172/1‑150 (MQ=255)

                                                            ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTATCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                          >  2:7061301/1‑150 (MQ=255)

                                                             tttCTCTGTCGGTCAGCTACAAGGCCCGCTTTGCGACCACGGGATTGTTTTCGATGTCTGGTTCCACCCTCGAGTCAGACAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         <  2:10576475/150‑1 (MQ=255)

                                                               tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       <  2:8980346/150‑1 (MQ=255)

                                                               tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       <  2:8691629/150‑1 (MQ=255)

                                                                 tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGCTTTACCGTCTTGAAGATCCTCCGCCGCGTCCTCA                                                                                     >  1:831102‑M1/1‑150 (MQ=255)

                                                                   tttCGGTCATATAAAATGCCCGCTTTGCTACCAGGGTATTTTTTTCGATGTGTGTTACCACCCTCGAGTCAGAAAATGCGCACCCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAA                                                                                   <  1:1876626‑M1/150‑1 (MQ=255)

                                                                   tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAA                                                                                   >  2:1245766‑M1/1‑150 (MQ=255)

                                                                   tttCGGCCAGCTAAATTGCCCGCTTTGCTACCAGGGTATTGTTTTCGTTGTCTGTTTCCACCCTCGAGACAGAAATTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAA                                                                                   <  1:10755370‑M1/150‑1 (MQ=255)

                                                                     tCGGTCAGATAAATTGCCCGCTTTGCTACCTGGGTTTTTTTTTCTTTGTCTGTTTCCACCCTCGTGTCAGAAATTGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACC                                                                                 <  1:4491914‑M1/150‑1 (MQ=255)

                                                                     tCGGTACGCTAAAATGCCCGATTTGCTACCCGGGTTTTGTTTTCTATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACC                                                                                 <  1:6502101‑M1/150‑1 (MQ=255)

                                                                         tCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                             <  2:11251710/150‑1 (MQ=255)

                                                                          cAGCTAAAATGCCCGCTTGGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            <  2:6922734/150‑1 (MQ=255)

                                                                           aGCTAAATTGCCCGCTTTGCTACCACGTTTTTTTTTTCGTTGTCTGTTTCCCCCCTCGAGTCAGAAATTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  1:10208259/150‑1 (MQ=255)

                                                                           aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  2:3901148/150‑1 (MQ=255)

                                                                           aGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTGTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                           <  2:3694134/150‑1 (MQ=255)

                                                                            gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGTTCGTCCGCCGCTTCCTCAAACCCGGTTTg                                                                          >  2:10207873/1‑150 (MQ=255)

                                                                            gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                          >  2:4826806/1‑150 (MQ=255)

                                                                             cTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         >  2:2276536/1‑150 (MQ=255)

                                                                             cTAAAATGCCCGCTTTGATACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                         <  2:461035/150‑1 (MQ=255)

                                                                              tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgcc                                                                        >  2:4902117/1‑150 (MQ=255)

                                                                               aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccg                                                                       <  2:2628844/150‑1 (MQ=255)

                                                                                atttGCCCGCTTTGCTACCTGGGGTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                      <  1:10971165/147‑1 (MQ=255)

                                                                                aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                      >  2:1901102/1‑150 (MQ=255)

                                                                                aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                      <  2:9852674/150‑1 (MQ=255)

                                                                                  aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                    >  2:6716571/1‑150 (MQ=255)

                                                                                     cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCACAAACCCAGTTTGCCGCCTGTc                                                                 >  1:1335008/1‑150 (MQ=255)

                                                                                     cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                 >  2:10881874/1‑150 (MQ=255)

                                                                                     cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCACAAACCCTGTTTTACGCCAGTc                                                                 >  1:9930714/1‑150 (MQ=255)

                                                                                     cccGCTTTGCTACCACGGGATTGTTGTCGAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                 <  2:9634914/150‑1 (MQ=255)

                                                                                      ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                <  2:3250197/150‑1 (MQ=255)

                                                                                            tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACACAAAACAGCGAACTGATTTAACGTCTTTAATATCGTCCGCCCCGTCCTCAAACCCAGGTTGCCGCCAGTCAGCAGct                                                          >  1:1044948/1‑150 (MQ=255)

                                                                                              cTACCAGGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                        <  1:2123812/150‑1 (MQ=255)

                                                                                              cTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAATATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                        >  1:8976584/1‑150 (MQ=255)

                                                                                              cTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                        <  2:950593/150‑1 (MQ=255)

                                                                                               tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACACCGAACAGATTTAACGTCTTTAAGATCGTCCGCCGCGTCCTCAAAACCTGTTTTCCGCCTGTCAGCAActgct                                                       >  1:5802881/1‑150 (MQ=255)

                                                                                                  cactgttttgttttgttTGTGTTTTTCCACGCTCGATTCAGTATATGCTCTACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                    <  1:2537927/143‑1 (MQ=255)

                                                                                                  cACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                    <  1:8635132/150‑1 (MQ=255)

                                                                                                  cACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGTGCGCAGACACCAAACAGCGGACGGCTTTAACGTCTTGAAGATCGGCCGCCGCTGCCACAAACCCAGGTTGCCGCCGCTCAGCAGATGCGGGc                                                    >  1:15507/1‑150 (MQ=255)

                                                                                                  cACGGGATTGTCTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGTAGATCGTCCGCCGCTTCCTCAAAACCAGTTTGCCGCCTGTCAGCATCTGCTGGc                                                    >  1:9249916/1‑150 (MQ=255)

                                                                                                         ttGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                             <  1:2794559/150‑1 (MQ=255)

                                                                                                         ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                             <  2:11662643/150‑1 (MQ=255)

                                                                                                          tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                            <  2:8051637/150‑1 (MQ=255)

                                                                                                           gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           <  2:7495551/150‑1 (MQ=255)

                                                                                                            ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          <  2:602955/150‑1 (MQ=255)

                                                                                                             tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                         >  2:6581750/1‑150 (MQ=255)

                                                                                                             tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                         >  2:6318218/1‑150 (MQ=255)

                                                                                                                 gATGTCTTTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                     <  1:11806577/150‑1 (MQ=255)

                                                                                                                  aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTAACGTCTATAAGATAGTCCGCCCCGTCCACAAAACCTGTTTGCCGCCAGTCAGCAACAGCGGGCGAGGTTcggtgcggtg                                    >  1:689163/1‑150 (MQ=255)

                                                                                                                   tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                   >  2:11981999/1‑150 (MQ=255)

                                                                                                                    gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  <  1:3344613/150‑1 (MQ=255)

                                                                                                                    gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAAAACCGAACAGATTTAACGTCATTAAGATAGTCCGCCGCTTCCTCAAACACAGTTTTCCGCCTGTCAGCATCTGAGGGCGATGTTAGTTGGGGTGAt                                  >  1:6318374/1‑150 (MQ=255)

                                                                                                                    gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACAGCGAACAGATTTAACGTCTTTAAGATCGTCCGCCGCGTCCGCAAAACCTGTGTGCCGCCCGTCAGCAACTGCTGGCGCTGGTCGTTGCGGTgct                                  >  1:9579294/1‑148 (MQ=255)

                                                                                                                     tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                 <  1:8001116/150‑1 (MQ=255)

                                                                                                                       tGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               <  2:1069255/150‑1 (MQ=255)

                                                                                                                         tttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  2:10355429/150‑1 (MQ=255)

                                                                                                                         tttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                             <  1:3137284/150‑1 (MQ=255)

                                                                                                                           tCCCCCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  1:6741904/150‑1 (MQ=255)

                                                                                                                             cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAGTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCatc                         >  2:3537362/1‑148 (MQ=255)

                                                                                                                                       tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGTGGTGATCACGCCAGCGCTATTAATc               >  2:11763908/1‑150 (MQ=255)

                                                                                                                                        cAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  1:4325985/150‑1 (MQ=255)

                                                                                                                                         aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             >  2:4418196/1‑150 (MQ=255)

                                                                                                                                           aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTGTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCGATTAATCTGcc           >  2:8339422/1‑150 (MQ=255)

                                                                                                                                            aaaTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          >  2:7357400/1‑150 (MQ=255)

                                                                                                                                              aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg        >  2:5581182/1‑150 (MQ=255)

                                                                                                                                                   cAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg   >  1:5460176‑M2/1‑149 (MQ=255)

                                                                                                                                                   cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg   <  1:4054982‑M2/150‑2 (MQ=255)

                                                                                                                                                    aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  2:619853‑M2/1‑148 (MQ=255)

                                                                                                                                                    aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  1:12043429‑M2/1‑148 (MQ=255)

                                                                                                                                                    aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  2:3049190‑M2/1‑148 (MQ=255)

ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  NC_000913/3828831‑3829128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`