Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,903,621 G→A 7.8% G27G (GGC→GGT bglB ← cryptic phospho‑beta‑glucosidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,903,6210GA 7.8% 2418.0 / 129.8 740G27G (GGC→GGTbglBcryptic phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base G (357/324);  new base A (29/29);  total (387/353)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 746 total aligned reads displayed.

AAGCCCATCTCGGCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCC  >  NC_000913/3903472‑3903770
                                                                                                                                                     |                                                                                                                                                     
aaGCCCATCTCGGCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTg                                                                                                                                                       >  1:8513581/1‑150 (MQ=255)
      aTCTCGGCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCt                                                                                                                                                 <  1:5684074/150‑1 (MQ=255)
        ctcGGCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTc                                                                                                                                               <  1:5256732/150‑1 (MQ=255)
          cGGCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCt                                                                                                                                             <  2:1466300/150‑1 (MQ=255)
            gCAAATAACGCGATATCTTCCGTGTATCGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGc                                                                                                                                           <  2:3913495/150‑1 (MQ=255)
            gCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGc                                                                                                                                           >  1:4556281/1‑150 (MQ=255)
            gCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGc                                                                                                                                           <  2:6772565/150‑1 (MQ=255)
            gCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTACCATCTTCCTGc                                                                                                                                           >  2:7354918/1‑150 (MQ=255)
              aaaTAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                                                                                                                         <  2:11835336/150‑1 (MQ=255)
              aaaTAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCa                                                                                                                                         >  2:71510/1‑150 (MQ=255)
              aaaTAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTACCATCTTCCTGCCa                                                                                                                                         <  2:9428929/150‑1 (MQ=255)
                aTAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAgg                                                                                                                                       >  2:2247184/1‑150 (MQ=255)
                    cgcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCAcc                                                                                                                                   >  1:2111263/1‑150 (MQ=255)
                    cgcgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCAcc                                                                                                                                   >  1:2328305/1‑150 (MQ=255)
                    cgcgATATCTTCCGAGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCAcc                                                                                                                                   >  1:8317202/1‑150 (MQ=255)
                      cgATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCtt                                                                                                                                 >  1:7884282/1‑150 (MQ=255)
                         tatCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCa                                                                                                                              <  2:6454086/150‑1 (MQ=255)
                         tatCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCa                                                                                                                              <  1:5049144/150‑1 (MQ=255)
                          atCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTACCATCTTCCTGCCAGGCACCTTCCAc                                                                                                                             >  1:6373598/1‑150 (MQ=255)
                           tCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTACCATCTTCCTGCCAGGCACCTTCCAcc                                                                                                                            >  2:12042155/1‑150 (MQ=255)
                            cTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCt                                                                                                                           <  1:11521449/150‑1 (MQ=255)
                             ttCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTg                                                                                                                          >  1:10600375/1‑150 (MQ=255)
                             ttCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTg                                                                                                                          >  2:4206658/1‑150 (MQ=255)
                              tCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGa                                                                                                                         <  1:3831727/150‑1 (MQ=255)
                                cGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGAtt                                                                                                                       <  2:9748934/150‑1 (MQ=255)
                                  ggTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTgg                                                                                                                     <  1:170374/150‑1 (MQ=255)
                                  ggTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTgg                                                                                                                     <  1:762726/150‑1 (MQ=255)
                                    tAACGGTGATAAAAATCGCTGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                                                                                                                   <  2:8282715/150‑1 (MQ=255)
                                    tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                                                                                                                   <  2:6664470/150‑1 (MQ=255)
                                    tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                                                                                                                   <  2:3010206/150‑1 (MQ=255)
                                    tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                                                                                                                   <  2:7764108/150‑1 (MQ=255)
                                    tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                                                                                                                   <  1:7063774/150‑1 (MQ=255)
                                    tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                                                                                                                   <  1:7335412/150‑1 (MQ=255)
                                    tAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCa                                                                                                                   <  1:11160631/150‑1 (MQ=255)
                                         gTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGt                                                                                                              <  2:8823712/150‑1 (MQ=255)
                                          tGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGtt                                                                                                             >  2:3315689/1‑150 (MQ=255)
                                           gATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTg                                                                                                            >  2:6953134/1‑150 (MQ=255)
                                           gATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTg                                                                                                            <  2:9051831/150‑1 (MQ=255)
                                            aTAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATACCTTTGCCATCTTCCTGCCAGGCACCTTCCGCCTGATTGGCAGCTGTTgc                                                                                                           <  1:9584673/150‑1 (MQ=255)
                                             tAAAAATCGATGGCGACATCTTTGATATTCTCTTTCGCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgcg                                                                                                          >  2:9640285/1‑150 (MQ=255)
                                              aaaaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgcgc                                                                                                         >  1:8034494/1‑150 (MQ=255)
                                              aaaaaTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTgcgc                                                                                                         <  2:10469931/150‑1 (MQ=255)
                                                  aTCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcc                                                                                                     <  1:3532271/150‑1 (MQ=255)
                                                   tCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAccc                                                                                                    >  2:10631303/1‑150 (MQ=255)
                                                   tCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAccc                                                                                                    >  1:9806202/1‑150 (MQ=255)
                                                    cGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCAcccc                                                                                                   >  2:5716054/1‑150 (MQ=255)
                                                       tGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCaaa                                                                                                <  1:911404/150‑1 (MQ=255)
                                                              aTCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGt                                                                                         >  1:277155/1‑150 (MQ=255)
                                                                 tttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTc                                                                                      >  1:12053325/1‑150 (MQ=255)
                                                                 tttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTc                                                                                      >  2:5490328/1‑150 (MQ=255)
                                                                 tttGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTc                                                                                      >  2:4055053/1‑150 (MQ=255)
                                                                    gATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTgg                                                                                   <  1:2972383/150‑1 (MQ=255)
                                                                    gATATTATCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCttg                                                                                   >  2:7891720/1‑148 (MQ=255)
                                                                            ctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCtt                                                                           <  2:11545678/150‑1 (MQ=255)
                                                                            ctTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTACCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCtt                                                                           >  2:11478436/1‑150 (MQ=255)
                                                                             tttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCttt                                                                          <  2:8626541/150‑1 (MQ=255)
                                                                             tttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCttt                                                                          <  2:2065991/150‑1 (MQ=255)
                                                                              ttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTc                                                                         >  1:871696/1‑150 (MQ=255)
                                                                              ttCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTc                                                                         >  1:9961593/1‑150 (MQ=255)
                                                                                 cccAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATa                                                                      >  1:10121315/1‑150 (MQ=255)
                                                                                 cccAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATa                                                                      >  1:10286788/1‑150 (MQ=255)
                                                                                 cccAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATa                                                                      <  2:10257155/150‑1 (MQ=255)
                                                                                   cAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAAt                                                                    >  2:12094007/1‑150 (MQ=255)
                                                                                      gATGCGCTGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTaa                                                                 <  2:11361450/150‑1 (MQ=255)
                                                                                      gATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTaa                                                                 <  1:11732870/150‑1 (MQ=255)
                                                                                       aTGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAAc                                                                <  2:1732595/150‑1 (MQ=255)
                                                                                          cgcgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTcc                                                             >  2:4434690/1‑150 (MQ=255)
                                                                                          cgcgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTcc                                                             <  2:11644488/150‑1 (MQ=255)
                                                                                           gcgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCt                                                            <  1:4816388/150‑1 (MQ=255)
                                                                                           gcgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCt                                                            >  2:4956115/1‑150 (MQ=255)
                                                                                            cgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCtt                                                           >  1:6895737/1‑150 (MQ=255)
                                                                                            cgGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCtt                                                           <  1:11314898/150‑1 (MQ=255)
                                                                                             ggTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTACCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCttt                                                          <  1:10004710/150‑1 (MQ=255)
                                                                                               ttCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTa                                                        >  1:1298528/1‑150 (MQ=255)
                                                                                               ttCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTa                                                        >  1:5923544/1‑150 (MQ=255)
                                                                                                tCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTAt                                                       >  1:3736218/1‑150 (MQ=255)
                                                                                                   aTTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGt                                                    >  1:6119874/1‑150 (MQ=255)
                                                                                                   aTTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGt                                                    >  1:8948049/1‑150 (MQ=255)
                                                                                                   aTTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTACCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGt                                                    >  2:4750864/1‑150 (MQ=255)
                                                                                                   aTTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATAACTTTGACATCTTACTGCAAGGAACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTAATAATTAACTAATTTTATctt                                                    >  2:3397953/1‑148 (MQ=255)
                                                                                                     ttttCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTa                                                  >  2:5845438/1‑150 (MQ=255)
                                                                                                      tttCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAg                                                 <  2:5205318/150‑1 (MQ=255)
                                                                                                            aTTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatg                                           <  2:3680321/150‑1 (MQ=255)
                                                                                                            aTTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatg                                           >  2:1087023/1‑150 (MQ=255)
                                                                                                              tACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAatgat                                         <  1:7417503/150‑1 (MQ=255)
                                                                                                                   cATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATa                                    >  1:3229517/1‑150 (MQ=255)
                                                                                                                   cATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATa                                    >  1:9414172/1‑150 (MQ=255)
                                                                                                                   cATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATa                                    >  1:1927591/1‑150 (MQ=255)
                                                                                                                             tAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTc                          >  1:8580538/1‑150 (MQ=255)
                                                                                                                                    gAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTgcg                   >  1:10944450/1‑150 (MQ=255)
                                                                                                                                       gTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCtt                >  1:4139644/1‑150 (MQ=255)
                                                                                                                                       gTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCtt                >  1:6540126/1‑150 (MQ=255)
                                                                                                                                          gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATc             >  2:7569335/1‑150 (MQ=255)
                                                                                                                                          gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATc             >  2:36009/1‑150 (MQ=255)
                                                                                                                                          gagaTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATc             >  1:3898077/1‑150 (MQ=255)
                                                                                                                                          gagaTCCCTTTACCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATc             <  2:2701110/150‑1 (MQ=255)
                                                                                                                                          gagaTCCCTTTACCATCTTCCTGCCAGGCACCATCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAAATAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATc             >  1:9314831/1‑150 (MQ=255)
                                                                                                                                             aTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGTGCTTATCTgc          <  2:11144414/150‑1 (MQ=255)
                                                                                                                                             aTCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgc          >  1:2538177/1‑150 (MQ=255)
                                                                                                                                              tCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTgcg         <  1:5329510/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGCCCATCTCGGCAAATAACGCGATATCTTCCGGGTAACGGTGATAAAAATCGATGGCGACATCTTTGATATTCTCTTTCCCCAGGATGCGCGGTTCCATTTTTCCCATTACGCCATGAGGCTGTAAATCTGAGGTCGAGATCCCTTTGCCATCTTCCTGCCAGGCACCTTCCACCTGATTGGCAGCTGTTGCGCCACCCCAAAGAAATGTTTCTGGAAATGCTTTCATAATTAACTCCTTTTATCGTTAGCGAATGATGGATAACAGCGGTTCACCTGCGCTTATCTGCGCCGTGCC  >  NC_000913/3903472‑3903770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: