Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,506 C→G 5.9% intergenic (‑140/+43) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,5060CG 5.9% 3097.2 / 60.9 931intergenic (‑140/+43)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (499/362);  new base G (13/56);  total (512/419)
Fisher's exact test for biased strand distribution p-value = 2.72e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 962 total aligned reads displayed.

CCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCG  >  NC_000913/3908357‑3908655
                                                                                                                                                     |                                                                                                                                                     
  aTACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTg                                                                                                                                                     <  1:2629774/150‑1 (MQ=255)
  aTACTCAGTGCAATCTGTTCCTCCTCGTGCCTGATGCACTTCGCTAATGAGGCTTCCCGTAGGCCGGATCAGGCGTTCCCGCCGCATCCGGCTAAGTGTTAACCGTGTAGAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTg                                                                                                                                                     <  2:7529975/150‑1 (MQ=255)
      tCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                 >  1:3945472/1‑150 (MQ=255)
      tCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                 >  2:6662305/1‑150 (MQ=255)
            cAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           >  1:2367770/1‑150 (MQ=255)
            cAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           >  1:1999865/1‑150 (MQ=255)
               tCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCAACCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                        >  2:8580287/1‑150 (MQ=255)
                cTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAACAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                       <  2:5867269/150‑1 (MQ=255)
                 ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                      >  1:1791142/1‑150 (MQ=255)
                      cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 >  1:6500431/1‑150 (MQ=255)
                       tCATCGTGCCTGATGCACTTCTCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGattg                                                                                                                                <  2:8902320/150‑4 (MQ=255)
                       tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                                >  2:6240318/1‑150 (MQ=255)
                          tCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                             >  1:11752193/1‑150 (MQ=255)
                          tCGTGCCCGATGCACGTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAATGTGTTAACCGTTTACCTATTCAAAAAGGGGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGaa                                                                                                                             <  2:6782316/150‑1 (MQ=38)
                                 tGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgtg                                                                                                                      >  1:3090152/1‑150 (MQ=255)
                                   aTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTc                                                                                                                    <  2:9127222/150‑1 (MQ=255)
                                      cACTTCGCTAATCAGGCTTACCGTAGGCCGTATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTAAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGc                                                                                                                 <  2:6962839/150‑1 (MQ=255)
                                         ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAat                                                                                                              >  1:9640267/1‑150 (MQ=255)
                                         ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAat                                                                                                              >  1:497953/1‑150 (MQ=255)
                                           cGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGGTCACGCCGCATCCGGCAAAGTGTTAACCGGGTACAGAGTCAAAAAGGCGCGCGGGCGGCCCCCTCGCCCCTCGGGGGCGAGGGGGAGGGTGAGGGGGAAGCCGTGGAAGCAatat                                                                                                            >  1:5567804/1‑150 (MQ=255)
                                                 tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCg                                                                                                      >  2:4781433/1‑150 (MQ=255)
                                                    ggCTTACCGTAGGCCGTATCAGGCGTTCACGCCGCATCCGGAAATGTGTTAACCGTGTACAGATTCAAAATGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgtgt                                                                                                   <  1:9041649/150‑1 (MQ=38)
                                                     gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGtt                                                                                                  >  2:11605031/1‑150 (MQ=255)
                                                       ttacgttAGGCCGGATCCGGCGTCCCCCCCGCACCCGGCAAAGTTTTAACCGTTTACAGATTCAAAAAGGCGCTCGTGCGGCCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTa                                                                                                <  1:8484911/144‑1 (MQ=37)
                                                        tACCGTTGGCCGGTTCAGGCGTTCACGCCGCATCGGTCAAATTGTTAACCGTTTAAAGAGTCAAAACGGCGCTCGGTCGTTCCCCCCGCCCCTCTGGGTTGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAt                                                                                               <  2:164141/150‑1 (MQ=255)
                                                        tACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAt                                                                                               <  2:11154563/150‑1 (MQ=255)
                                                         aCCGTAGGCCGGATCAGGCGTTCACGCCGCAGCCGGAAATGTTTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAtt                                                                                              <  2:3735412/150‑1 (MQ=39)
                                                          ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGACAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                                             >  2:7214133/1‑150 (MQ=255)
                                                          ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                                             >  2:12238833/1‑150 (MQ=255)
                                                           cGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAATAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGGGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttct                                                                                            >  2:221197/1‑150 (MQ=255)
                                                            gTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCATAGTGTTAACCGTGTACAGAGACAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttctt                                                                                           <  2:10992473/150‑1 (MQ=255)
                                                             tAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                                                                                          >  1:1624041/1‑150 (MQ=255)
                                                             tAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                                                                                          >  2:439544/1‑150 (MQ=255)
                                                                gCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCa                                                                                       >  2:7998534/1‑150 (MQ=255)
                                                                  cGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaa                                                                                     >  2:8223926/1‑150 (MQ=255)
                                                                   ggatgcggCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaa                                                                                    >  1:7239079/7‑150 (MQ=38)
                                                                   ggATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaaa                                                                                    >  2:2616345/1‑150 (MQ=255)
                                                                      tCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaaca                                                                                 >  2:7445136/1‑150 (MQ=255)
                                                                        tggCTTTCCCGCCGCATCGGGAAATGTTTTAACGGTGAACAGAGTAAAACTGGGGCGGGGTGGGCCCCCTCGCCCTTGTGGGTTGTGGTTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaac                                                                               <  1:12273930/149‑1 (MQ=255)
                                                                        aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaac                                                                               >  1:5110133/1‑150 (MQ=255)
                                                                        aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGACAAAAAGGCGCTCGGTCGGGCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaac                                                                               <  2:392615/150‑1 (MQ=255)
                                                                          gCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgc                                                                             >  1:7507550/1‑150 (MQ=255)
                                                                           cGTTCACGCCGCATCCGGCAATGTTTTAACCGTGTACAGAGTCAAAATGGCGCTGGGTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgcg                                                                            <  2:11931996/150‑1 (MQ=39)
                                                                              tCACGCCGCATCCGGCAATGTGTTAACCGTTTACAGTGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACgcgcgc                                                                         <  1:10984014/150‑1 (MQ=39)
                                                                              tCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACgcgcgc                                                                         <  2:1998147/150‑1 (MQ=255)
                                                                               cccgccgcACCCGCCAATTTGTTCCCCGTGTCCAGAGTCAAAACGGGGCTGGGGGGGTCCCCCCGCCCGCGTGGGGTGTGGGTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGcc                                                                        <  1:3442854/148‑1 (MQ=255)
                                                                                aCGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCa                                                                       >  1:617593/1‑150 (MQ=255)
                                                                                  gccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGa                                                                     >  2:5633847/1‑150 (MQ=255)
                                                                                  gccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGa                                                                     >  1:10642055/1‑150 (MQ=255)
                                                                                   ccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGaa                                                                    >  2:4631725/1‑150 (MQ=255)
                                                                                   ccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGaa                                                                    >  2:303323/1‑150 (MQ=255)
                                                                                    cgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAAGCGCGCCAGAAt                                                                   >  2:6370882/1‑150 (MQ=255)
                                                                                     gcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATg                                                                  >  2:10487880/1‑150 (MQ=255)
                                                                                        gccGGCATTGTGTTAACCGTGCACAGCGAAAAAATGGCGCTGGGTCGGTCCCCTCGCCCCTGTGGGGTGTGGGTTGGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                               <  1:11460518/149‑1 (MQ=255)
                                                                                          cGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcag                                                             >  2:8358750/1‑150 (MQ=255)
                                                                                          cGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcag                                                             >  1:8079093/1‑150 (MQ=255)
                                                                                           ggCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagc                                                            >  2:5003241/1‑150 (MQ=255)
                                                                                           ggCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagc                                                            >  2:1790055/1‑150 (MQ=255)
                                                                                           ggCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagc                                                            >  1:4564336/1‑150 (MQ=255)
                                                                                           ggCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagc                                                            >  2:9349378/1‑150 (MQ=255)
                                                                                               cattgtTAACCGTTTACAGAGACAAAACGGCGCGCGGTCGGTCCCCTCGCCCCCCTGGGGATTGGGTTAGGGTGGGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcagca                                                        <  2:32631/147‑1 (MQ=255)
                                                                                                   gtTAACCGTGTACAGAGTCAAAAAGGCGCGGGGTCGGTCCCCGCGCCCCTGTGGGGAGTGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTAc                                                    <  1:11650213/150‑1 (MQ=255)
                                                                                                    ttAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACg                                                   >  2:5229748/1‑150 (MQ=255)
                                                                                                      aaCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca                                                 >  1:8713861/1‑150 (MQ=255)
                                                                                                       aCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcac                                                >  2:287069/1‑150 (MQ=255)
                                                                                                          gtgtACAGAGTCATAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                             <  2:10061978/150‑1 (MQ=255)
                                                                                                          gtgtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                             <  2:10214609/150‑1 (MQ=255)
                                                                                                              aCAGAGTCAAAACGGCGCTCGGTCGGTCCCCGCGCCCCTCTGGGTAGTGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTaa                                         <  1:8315357/150‑1 (MQ=255)
                                                                                                              aCAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTaa                                         <  1:1214051/150‑1 (MQ=255)
                                                                                                               catagTCAAAACGGCGCTGGGTCGGTCCCCCCCCCCCTCGGGGGAGAGGTTTCGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAAt                                        <  2:3564981/147‑1 (MQ=255)
                                                                                                                  cgTCAAAACGGCGCTCGGTCGGGCCCCCCGCCCCGGGGGGGTGTGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                     <  1:5414476/149‑1 (MQ=255)
                                                                                                                  agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                     <  2:8124682/150‑1 (MQ=255)
                                                                                                                  agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                     <  1:11265072/150‑1 (MQ=255)
                                                                                                                  agTAAAAATGGCGCTCGGTCGGTCCCCACGCCCCTCTGGGGTGAGGGTTAGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                     <  1:286782/150‑1 (MQ=255)
                                                                                                                   ctCAAAACGGCGCTCGGTCGGCCCCCTCGCCCCTGTGGGGTGTGGGTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATc                                    <  2:4182214/149‑1 (MQ=255)
                                                                                                                    acAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGATGGGAAAACCTTGTCAGCAATATCTACCGTGTTTATTCTTCGCATAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCa                                   <  2:11789029/149‑1 (MQ=255)
                                                                                                                      aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAAt                                 >  1:9220973/1‑150 (MQ=255)
                                                                                                                      aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAAt                                 >  1:893604/1‑150 (MQ=255)
                                                                                                                       aaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATa                                >  1:42171/1‑150 (MQ=255)
                                                                                                                       aaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATa                                >  1:11150722/1‑150 (MQ=255)
                                                                                                                       aaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATa                                >  1:10511369/1‑150 (MQ=255)
                                                                                                                        aaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAc                               >  1:226646/1‑150 (MQ=255)
                                                                                                                          aGGCGCTCGGTCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATAccc                             <  1:10046398/150‑1 (MQ=255)
                                                                                                                             cgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCgg                          >  2:11374807/1‑150 (MQ=255)
                                                                                                                               cTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCACAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc                        >  1:6908977/1‑150 (MQ=255)
                                                                                                                               cTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc                        <  1:10178893/150‑1 (MQ=255)
                                                                                                                                  ggtcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAg                     >  2:9803257/1‑150 (MQ=255)
                                                                                                                                    tcggtcCCCTCGCCCCTGTGGGTTGAGTGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc                   <  2:3389570/150‑1 (MQ=255)
                                                                                                                                    tcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc                   >  1:7935258/1‑150 (MQ=255)
                                                                                                                                       gtcCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCaa                <  1:586952/150‑1 (MQ=255)
                                                                                                                                         acccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACACCGGCCCAGGCCAAtt              >  2:600653/2‑150 (MQ=255)
                                                                                                                                            cccGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGCAAACCCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  1:3586220/148‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGTTGTGGGTTTGGTTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  1:9515173/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           >  2:7713694/1‑150 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           >  2:10775171/1‑150 (MQ=255)
                                                                                                                                             gcGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctg          <  2:1442364/149‑1 (MQ=255)
                                                                                                                                                ccccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCa       >  2:12018826/1‑150 (MQ=255)
                                                                                                                                                  ccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAtt     >  2:5427698/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTcc   >  1:4775002/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTcc   >  2:9488914/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCG  >  NC_000913/3908357‑3908655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: