Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,482 A→C 5.4% intergenic (+37/+50) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4820AC 5.4% 1661.1 / 13.2 570intergenic (+37/+50)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base A (185/330);  new base C (37/8);  total (232/338)
Fisher's exact test for biased strand distribution p-value = 1.41e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 691 total aligned reads displayed.

ACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAAC  >  NC_000913/3959333‑3959631
                                                                                                                                                     |                                                                                                                                                     
 cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                                                      <  2:762309/150‑1 (MQ=255)
  gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCGCTACCCCagg                                                                                                                                                     >  1:10420427/1‑150 (MQ=255)
    gCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCTACCAAaggga                                                                                                                                                   >  2:1994945/1‑150 (MQ=255)
      aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                                                 <  2:4750569/150‑1 (MQ=255)
       aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagag                                                                                                                                                <  2:5421373/150‑1 (MQ=255)
       aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagag                                                                                                                                                <  1:3309881/150‑1 (MQ=255)
        cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAATCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                               <  2:3829385/150‑1 (MQ=255)
        cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                               <  2:5193848/150‑1 (MQ=255)
        cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagagg                                                                                                                                               <  1:3093871/150‑1 (MQ=255)
        cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGAGATTGAGCAGGTAGGTAAGAAACTGCGAGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagtgagagg                                                                                                                                               <  2:2448938/150‑1 (MQ=255)
         tGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggg                                                                                                                                              >  1:12147988/1‑150 (MQ=255)
         tGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggg                                                                                                                                              <  2:7375798/150‑1 (MQ=255)
         tGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggg                                                                                                                                              <  2:830557/150‑1 (MQ=255)
            gTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAgggcgagggaga                                                                                                                                           >  1:1137759/1‑150 (MQ=255)
             tGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCACTCCCACAGGGAGCGGGAGaa                                                                                                                                          >  1:1718781/1‑150 (MQ=255)
                 gtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCCCTCACCCACAGGGAGGGGGGGAAAaca                                                                                                                                      >  2:606151/1‑150 (MQ=255)
                  tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCTCCCACAGGGAGAGGGAGAAAacac                                                                                                                                     >  1:7054252/1‑150 (MQ=255)
                   gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACt                                                                                                                                    >  1:7951063/1‑150 (MQ=255)
                         aGCGATTCGCAGCCATGCGATTGAGCAGGTTGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCTAGGc                                                                                                                              <  2:8889452/150‑1 (MQ=255)
                           cGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAAAACTCAAGGCCt                                                                                                                            >  1:2819299/1‑150 (MQ=255)
                            gATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACTCTCAAGGCCtt                                                                                                                           <  2:11684651/150‑1 (MQ=255)
                            gATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCta                                                                                                                           >  2:7881365/1‑149 (MQ=255)
                            gATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTATCCCACAGGGAGAGGGAGAAAACACTCAAGGCCtt                                                                                                                           >  1:10005052/1‑150 (MQ=255)
                              ttCGCAGCCATGCGATTGAGCAGGTAGGTAAGAATCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtct                                                                                                                         <  1:6174932/150‑1 (MQ=255)
                              ttCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGCCACACCCCCACCCACACCCCCAGGGGGGGGGAGAAAAAACACAAGGccgtat                                                                                                                         >  2:396977/1‑146 (MQ=255)
                                cGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTcc                                                                                                                       <  1:3487712/150‑1 (MQ=255)
                                  cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                     <  2:6127811/150‑1 (MQ=255)
                                    gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTTCTGTTGAGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                   <  1:4237335/150‑1 (MQ=255)
                                        tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                                               <  1:11670700/150‑1 (MQ=255)
                                         gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTACCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGGGCAgg                                                                                                              >  2:7043054/1‑150 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCCCAGGGAGAGGGGGAAAACACTCAAGGCCTTCTCCTGGAGAAGGcc                                                                                                            >  1:11861201/1‑150 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGTGGGAGAAAACACTCTAGGCCTTCTCCTGGAGAAGGcc                                                                                                            <  2:464130/150‑1 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGcc                                                                                                            <  1:5618323/150‑1 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGcc                                                                                                            <  1:9558135/150‑1 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCTCCCACACGGAGAGGGGGAAAACACACAAGGCCATCTCCTGGGGAAGGcc                                                                                                            >  1:2664209/1‑150 (MQ=38)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCTCACCCACAGGGAGAGGGAGAAAAAACTCAAGGCCTTCTACTGGAGGACGcc                                                                                                            >  2:4884863/1‑150 (MQ=255)
                                              tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                         <  1:990897/150‑1 (MQ=255)
                                               gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTga                                                                                                        <  1:8482680/150‑2 (MQ=255)
                                                aGCAGGTAGGTAAGAAACTGCACGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGCCTTgag                                                                                                       <  2:4464381/150‑3 (MQ=255)
                                                     gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAg                                                                                                  >  1:10186695/1‑150 (MQ=255)
                                                        ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCGCACCCCCCGGGAGAGGGAGAAAACACTAAAGGCCTTATCCTGGGGCAGGCCGTGCTATTATTTg                                                                                               >  1:8662702/1‑150 (MQ=255)
                                                         gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                                              <  1:10554567/150‑1 (MQ=255)
                                                         gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCACCCACACCCACAGGGCGAGGGAGAAAAAACACAAGGCCTTCTCCAGGAGAAGGCCGTGCTATTAGtttc                                                                                              >  1:4732333/1‑148 (MQ=255)
                                                          taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                             <  1:831576/150‑1 (MQ=255)
                                                           aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACTCCCACCCGGGGCGGGAGCAAACACACAATGCCATCTCCCGGCGAAGGCCGGGCAATTAGTTTCgg                                                                                            >  2:9634438/1‑150 (MQ=255)
                                                           aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCAAAACCACCGGGAGCTGGAGAAAAAACACAAGGCCGTATCCCGGAGAAGGCCTTGCGATTATTTTCgg                                                                                            >  2:11494724/1‑150 (MQ=255)
                                                           aaGAAACTGCGCGGCTATATGACAGATAGGACACGTATTGCTGTTGCGGGTTCAGTGCGCGCTGATGCCCGCACCCCGACCCTCTCCCACAGGGCGCGGGGGCACACACGCGAGGCCGTCGCCTGGGGGGGGCCGTGGTCGTAGTTGCgg                                                                                            >  2:11904710/1‑150 (MQ=255)
                                                             gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCACACCCCCACGGAGGGGGAGAAAACACACAAGGCCGACACCGGGAGAACGCCGTGCGATTAGTTGCGGTa                                                                                          >  1:11238952/1‑150 (MQ=255)
                                                               aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCCCCCACACCCCCAGGGAGAGGGAGAAAAAAAACAATGCCGTCTACAGGGGCAGGCCGTGCGAATATTTTCGGttaa                                                                                        >  1:9643309/1‑147 (MQ=255)
                                                                aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                                                                       <  1:5271802/150‑1 (MQ=255)
                                                                  tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCCCACCCCCACCCACGCCCCCCCGGCGGGGGCGAAAAAACACAACGCCCTCTCCAGGGGCAGGCCGGGCGATTAGTTGCGGTGCaccc                                                                                     >  2:1516689/1‑147 (MQ=255)
                                                                   gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc                                                                                    <  1:6048493/150‑1 (MQ=255)
                                                                   gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc                                                                                    <  1:2314167/150‑1 (MQ=255)
                                                                     gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACACCCACAGGGAGAGGGAGAAAAAACTCAAGGCCATCTCCGGGGGTAGGCCGTGGTATTATTTTCGGGTCAGCAcca                                                                                  >  1:5125303/1‑149 (MQ=25)
                                                                     gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCGACCCACTCCCACACGGCGAGGGAGAAAAAACACAAGGCCAACTACAGGAGAAGGCCTTGCTATTATTTGCGGTACAGCAcca                                                                                  >  2:1271957/1‑149 (MQ=255)
                                                                              tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                         <  1:147091/150‑1 (MQ=255)
                                                                                aCAGATATGACCCGTATTGCTGTTGCGGGTTAAGGGCGCGCGGATGCCCTCCCCCCGACCCGCTCCCCCAGGGAGAGGGCGAAAACACTCCCGGCCGGCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                       <  2:3198450/150‑1 (MQ=255)
                                                                                aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                       <  2:5543887/150‑1 (MQ=255)
                                                                                    atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                   <  2:5792113/150‑1 (MQ=255)
                                                                                       tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCTCAACCCGACCCACTCCCACACGGGGAGGGAGAAAAAACTCAAGGCCTTATCCGGGAGAAGGCCGTGCTATTAGTTTCGGTTCAGCACCGTACAGTTGTGGTATCCGa                                                                >  2:10454368/1‑150 (MQ=255)
                                                                                         aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCCCCCACACCCACAGGGCGCGGGGGAAAAAAAAAAAAGCCATCTCCAGGAGAAAGCCATTCAATTAGTTTCGGGAAAGCACCAAAAAGATTTTatataccaaa                                                              >  2:9492193/1‑144 (MQ=255)
                                                                                         aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACTCCCACACGGAGAGGGAGAAAAAAAACAAGGCCTTCACCTGGAGAAGGCCGTGCGAATAGTTGCGGTACAGCAACATAAATATGTGTTATACGAAc                                                              >  1:8846264/1‑150 (MQ=255)
                                                                                          aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACt                                                             <  1:10742759/150‑1 (MQ=255)
                                                                                            cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                           <  2:10784207/150‑1 (MQ=255)
                                                                                             ggaTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                                                          <  1:10927355/148‑1 (MQ=255)
                                                                                             gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                                                          <  1:3721046/150‑1 (MQ=255)
                                                                                              tagtGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTATTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                         <  2:11299511/147‑1 (MQ=255)
                                                                                              tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                         <  1:4368323/150‑1 (MQ=255)
                                                                                              gaTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                         <  2:10139122/149‑1 (MQ=255)
                                                                                                ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                                                       <  2:3434090/150‑1 (MQ=255)
                                                                                                ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCTACCACACGGAGAGGGAGAAAAAACACAACGCCATCACCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCATAAAGATGTGATATACGAACGGGgtgt                                                       >  2:6636341/1‑150 (MQ=37)
                                                                                                 ggCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACATGTAGAGGGAGTAAACACTCAAGGCCTTCTTCTGGAGAAGGCCTTGCTATTTGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                      <  1:9316734/149‑1 (MQ=39)
                                                                                                        gCGGGTTAAGTGCGCGCTGCTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCggg                                               <  1:7444442/150‑1 (MQ=255)
                                                                                                        gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCggg                                               <  2:2177951/150‑1 (MQ=255)
                                                                                                            gTTAAGTGCGCGCTGATTCCCAAACCCCCACCCAAAACCCCACGGGGAGGGGGAAAAAAAAAAAAGCCATCTACCGGGGAAGGCCGTTCGAATATTTTCGGTTCAACACCAAAATGAAGTGATATAACAACTGGGTGTGCAGCGGGcccg                                           >  2:4228175/1‑148 (MQ=255)
                                                                                                            gTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCgg                                           <  2:9716559/150‑1 (MQ=255)
                                                                                                                aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                       <  1:9095650/150‑1 (MQ=255)
                                                                                                                 gTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGc                                      <  1:4991132/150‑1 (MQ=255)
                                                                                                                  tGCGCGCTGATGCCCGCACCCCGACCCTCAACCACAGGGGGCGGGAGAAAACACACAAGGCCTTCTCCTGGTGAAGGCCTTGCTATTAGTTGCGGTTAAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGcg                                     >  2:5030112/1‑149 (MQ=255)
                                                                                                                   gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                    <  1:2438802/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                  <  1:5056581/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                  <  1:2222479/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                  <  2:9161429/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                  <  1:656835/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                  <  2:10908669/150‑1 (MQ=255)
                                                                                                                           aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                            <  2:7715926/150‑1 (MQ=255)
                                                                                                                           aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                            <  2:8640070/150‑1 (MQ=255)
                                                                                                                               ccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCGGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGCTATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACa                        >  2:9397307/1‑150 (MQ=255)
                                                                                                                                  cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                     <  2:7779865/150‑1 (MQ=255)
                                                                                                                                      ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCCGGCCTGCGCCTGGAGAAGGCCTTGCGATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                 <  1:385578/150‑1 (MQ=255)
                                                                                                                                      ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                 >  1:7704125/1‑150 (MQ=255)
                                                                                                                                       cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                <  1:6809731/150‑1 (MQ=255)
                                                                                                                                         aCCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAcc              <  1:1989597/150‑1 (MQ=255)
                                                                                                                                           ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc            >  2:3062631/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc            >  2:6528637/1‑150 (MQ=255)
                                                                                                                                              ctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACttt         >  1:5904855/1‑150 (MQ=255)
                                                                                                                                               tcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTa        <  2:12121905/150‑1 (MQ=255)
                                                                                                                                                cccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAt       <  2:6339365/150‑1 (MQ=255)
                                                                                                                                                 ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc      >  2:2834089/1‑150 (MQ=255)
                                                                                                                                                    caGGGAGAGGTAGAAAACACTCAAGGCCTTCTCCTGGATAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTTATATCCGAACTGGTTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa   <  1:256444/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAAC  >  NC_000913/3959333‑3959631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: