Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,005,308 A→T 5.9% A149A (GCA→GCT pldA → outer membrane phospholipase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,005,3080AT 5.9% 2566.2 / 17.6 801A149A (GCA→GCTpldAouter membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base A (361/360);  new base T (2/72);  total (365/436)
Fisher's exact test for biased strand distribution p-value = 4.49e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 823 total aligned reads displayed.

GGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGT  >  NC_000913/4005159‑4005457
                                                                                                                                                     |                                                                                                                                                     
 ggATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAg                                                                                                                                                      <  1:6423331/150‑1 (MQ=255)
 ggATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCAATTACCGTTTTGCAg                                                                                                                                                      >  1:7796252/1‑150 (MQ=255)
   aTTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGt                                                                                                                                                    <  2:7581689/150‑1 (MQ=255)
       tAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGa                                                                                                                                                <  1:10005900/150‑1 (MQ=255)
          gCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGc                                                                                                                                             >  1:6072729/1‑150 (MQ=255)
          gCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGc                                                                                                                                             >  1:7914472/1‑150 (MQ=255)
           cccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCTAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCt                                                                                                                                            >  2:4860940/1‑150 (MQ=255)
           cccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCt                                                                                                                                            >  1:2035124/1‑150 (MQ=255)
            ccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTg                                                                                                                                           >  2:9413244/1‑150 (MQ=255)
               aaCTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTgcgc                                                                                                                                        <  2:8797968/150‑1 (MQ=255)
               aaCTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTgcgc                                                                                                                                        <  1:2156616/150‑1 (MQ=255)
                 cTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGa                                                                                                                                      >  2:5391046/1‑150 (MQ=255)
                 cTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGa                                                                                                                                      >  1:10676333/1‑150 (MQ=255)
                  tCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAt                                                                                                                                     >  2:6108978/1‑150 (MQ=255)
                   cGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtg                                                                                                                                    >  1:11290068/1‑150 (MQ=255)
                    ggTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgt                                                                                                                                   <  1:9248206/150‑1 (MQ=255)
                    ggTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgt                                                                                                                                   <  2:1156168/150‑1 (MQ=255)
                    ggTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgt                                                                                                                                   <  2:12188572/150‑1 (MQ=255)
                     gtgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgtg                                                                                                                                  <  2:4034611/150‑1 (MQ=255)
                      tgtTGGGTGCGTCTTATACGCAACAATCCTGGTGGCAACTGTCCACGCGCGAAGAGTCTTCACCGTTTCGTGAAACCACCTCCGAACCGCAATTGGTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                                                                                                                                 <  2:1810534/150‑1 (MQ=255)
                      tgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                                                                                                                                 <  2:8601936/150‑1 (MQ=255)
                      tgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTgg                                                                                                                                 <  1:5243242/150‑1 (MQ=255)
                       gtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGga                                                                                                                                <  1:7225138/150‑1 (MQ=255)
                        ttGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTATCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTTCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGgag                                                                                                                               <  1:5482110/150‑1 (MQ=255)
                           ggTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATg                                                                                                                            <  2:5554617/150‑1 (MQ=255)
                            gTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgg                                                                                                                           <  2:7483314/150‑1 (MQ=255)
                            gTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgg                                                                                                                           >  1:5140089/1‑150 (MQ=255)
                            gTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgg                                                                                                                           <  2:3607792/150‑1 (MQ=255)
                            gTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgg                                                                                                                           <  2:1511434/150‑1 (MQ=255)
                               cGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGt                                                                                                                        <  2:9003926/150‑1 (MQ=255)
                               cGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGt                                                                                                                        <  1:6614830/150‑1 (MQ=255)
                                gTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGta                                                                                                                       >  1:2373447/1‑150 (MQ=255)
                                   ttATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATaa                                                                                                                    <  1:4436706/150‑1 (MQ=255)
                                   ttATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATaa                                                                                                                    <  2:517265/150‑1 (MQ=255)
                                   ttATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATaa                                                                                                                    >  1:6820168/1‑150 (MQ=255)
                                   ttAGACGCAAAAATCCGGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATaa                                                                                                                    <  2:1363736/150‑1 (MQ=255)
                                      taCGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCa                                                                                                                 <  1:852994/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              <  2:8439188/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              >  1:3684511/1‑150 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              >  2:7780543/1‑150 (MQ=255)
                                               aTCCTGGTGGCAACTGTCCAATAGCGAAGAGTATTCACCGTTTCGTGAAACCAACTACGAACCGAAATTGTTCCTCGTTTTTGCCACCGTTTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGTGATGGGGTATAACCACGACTCTaa                                                                                                        <  2:11943837/150‑1 (MQ=255)
                                                                ccAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTccgacccga                                                                                       <  2:2730520/150‑1 (MQ=255)
                                                                acAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAAATACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCTTTTTGCAGGTTGGACGCTGCGCGATGTGTAGATGGGGTATAACCACGACTCTAACGGGCGTTccgacccga                                                                                       <  2:10876047/149‑1 (MQ=255)
                                                                 cAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTccgacccgac                                                                                      >  1:7010581/1‑150 (MQ=255)
                                                                  aaTAGCGAAGAGTCTTCACCGTTTCGAGAAACCAACTACGAACCGCCATTGTTCCTCGGTTGTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCcgacccgacc                                                                                     >  1:11712839/1‑150 (MQ=255)
                                                                   aTAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCt                                                                                    >  2:243776/1‑150 (MQ=255)
                                                                    tAGCGAAGAGTCTTCACGGTTTCGTTAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTc                                                                                   <  2:5820271/150‑1 (MQ=255)
                                                                     aGCGAAGAGTCTTCACCGTTTCGTCAAACCAACTACGAACCGCAATTTTTCCTGGTTTTTGCCACCGATTACCTTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTcc                                                                                  <  1:9713681/150‑1 (MQ=255)
                                                                       ctatgagTCTTCACCGTTTCGTTAAACCAACACCCACCCGCAATTGTTCCTGGGTTTTGCCACCGATAACCGTTTTGCAGGTTGGACGCTGCGCTTTGTGGAGTTGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCg                                                                                <  1:10775842/146‑1 (MQ=255)
                                                                        gAAGAGTCTTCACGGTTTCGTGAAACCAACTACGACCCGCATTTGTTCCTCGGTTTTGCCACCGATTCCGGTTTGGCTGGTTGGACGGTGGGCGATGTGGCGAGGGGGTATACCCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGc                                                                               <  1:8820736/150‑1 (MQ=255)
                                                                        gAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTCACGCGCGTTCCGACCCGACCTCCCGc                                                                               >  2:6602652/1‑150 (MQ=255)
                                                                           gcgTCTTCACCGTTTCGTGAAACCAACTCCGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGTTGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGc                                                                            <  1:9436015/148‑1 (MQ=255)
                                                                            agTCTTCACCTTTTCGTTAAACAAACTACCAACCGCATTTGTTCCTCGTTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGTTGTGTTGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCt                                                                           <  1:4150365/150‑1 (MQ=255)
                                                                            agTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCt                                                                           >  1:11847050/1‑150 (MQ=255)
                                                                               cTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGa                                                                        >  2:8187016/1‑150 (MQ=255)
                                                                                ttCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGaa                                                                       >  2:9609275/1‑150 (MQ=255)
                                                                                 aaaCCGTTTCGTGAAACCAACTACGAACCGCATTTGTTCCTCGGTTTTGCCACCGATTACCTTTTTGCAGGTTGGACGCTGCGCGATGTGGATATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAAc                                                                      <  2:11317097/148‑1 (MQ=255)
                                                                                  cACCGTTTCGTGAACCCACCGCCGACCCGCAATTGGTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAAcc                                                                     <  2:11086362/150‑1 (MQ=255)
                                                                                  cACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAAcc                                                                     <  2:2056477/150‑1 (MQ=255)
                                                                                  cACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAAcc                                                                     >  2:10626550/1‑150 (MQ=255)
                                                                                    ccGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGc                                                                   >  1:1050301/1‑150 (MQ=255)
                                                                                    ccGTTTCGTGAAACCAACTACGAACCGCAATTGTGCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGc                                                                   <  1:4779372/150‑1 (MQ=255)
                                                                                     cGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGcc                                                                  <  2:7970547/150‑1 (MQ=255)
                                                                                      gTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCt                                                                 <  1:4616767/150‑1 (MQ=255)
                                                                                       tttCGTTAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCTTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCtt                                                                <  1:2988093/150‑1 (MQ=255)
                                                                                       tttCGTTAAACCAACTACGAACCGCAATTGTCCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCGGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCtt                                                                <  1:5625408/150‑1 (MQ=255)
                                                                                          cttGAAACCAACTACCAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTtat                                                             <  2:440182/148‑1 (MQ=255)
                                                                                           gTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTtata                                                            >  1:6282427/1‑150 (MQ=255)
                                                                                            tGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGTAGATGGGGTATACCCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATAc                                                           <  1:3411897/150‑1 (MQ=255)
                                                                                            tGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATAc                                                           <  1:1302820/150‑1 (MQ=255)
                                                                                             gAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCTTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACt                                                          <  1:11591193/150‑1 (MQ=255)
                                                                                              aaaCCAACTACGAACCGCATTTGTTCCTCGGTTTTGCCACCGTTTACCGTTTTGCTGGTTGGACGCTGCGCTTTGTGGATTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTc                                                         <  2:100615/150‑1 (MQ=255)
                                                                                              aaaCCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCCGGTTGGACGCTGCGCGATGTGGAGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTc                                                         <  2:10359796/150‑1 (MQ=255)
                                                                                              aaaCCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTc                                                         >  1:9863419/1‑150 (MQ=255)
                                                                                                aCCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGTTTGGACGCTGCGCGATGTGTATATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGc                                                       <  2:9807058/150‑1 (MQ=255)
                                                                                                   aaCTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTg                                                    >  2:4978069/1‑150 (MQ=255)
                                                                                                        cGAACCGCATTTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGc                                               <  1:5955972/150‑1 (MQ=255)
                                                                                                            ccGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGaa                                           <  1:8270761/150‑1 (MQ=255)
                                                                                                             cGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGaaa                                          >  2:3226416/1‑150 (MQ=255)
                                                                                                              gCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGaaaa                                         <  2:6887974/150‑1 (MQ=255)
                                                                                                               cAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAAc                                        >  2:3775956/1‑150 (MQ=255)
                                                                                                                aaTTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACg                                       <  2:9196509/150‑1 (MQ=255)
                                                                                                                    gTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTaa                                   <  1:3962536/150‑1 (MQ=255)
                                                                                                                    gTTCCTCGGTTTTGCAACAGATTACCTTTTGGCTGGTTGTACGCTGCGCGTTGTGGTTATGGGTTATACCCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTaa                                   <  1:11929049/150‑1 (MQ=255)
                                                                                                                        cTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActgg                               <  2:2948813/150‑1 (MQ=255)
                                                                                                                        cTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActgg                               >  1:2816733/1‑150 (MQ=255)
                                                                                                                         tCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggc                              >  1:10213056/1‑150 (MQ=255)
                                                                                                                         tCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggc                              >  1:7702741/1‑150 (MQ=255)
                                                                                                                          cGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggct                             >  2:2155852/1‑150 (MQ=255)
                                                                                                                            gTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggctgg                           <  1:1606338/150‑1 (MQ=255)
                                                                                                                              tttGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                         <  1:9040136/150‑1 (MQ=255)
                                                                                                                                  ccACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCCCGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAg                     <  2:7155186/150‑1 (MQ=255)
                                                                                                                                   cACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGt                    >  1:2600175/1‑150 (MQ=255)
                                                                                                                                    aCCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTg                   >  2:4616050/1‑150 (MQ=255)
                                                                                                                                      cGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGaa                 <  1:12072850/150‑1 (MQ=255)
                                                                                                                                       gATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTATATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAg                >  2:4190243/1‑150 (MQ=255)
                                                                                                                                           aCCGTTTTGCAGGTTGGACGCGGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGt            <  2:2273320/150‑1 (MQ=255)
                                                                                                                                             cGTTTTGCTGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTgg          <  1:7447337/150‑1 (MQ=255)
                                                                                                                                                 ttGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtg      <  1:1102740/150‑1 (MQ=255)
                                                                                                                                                     aGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtggt  <  2:6413930/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGT  >  NC_000913/4005159‑4005457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: