Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,297,107:1 +C 100% coding (538/624 nt) ccmA ← heme export ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,297,1071.C90.0% 394.5 / 39.2 110coding (538/624 nt)ccmAheme export ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base . (4/7);  new base C (41/58);  total (45/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 129 total aligned reads displayed.

CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACG  >  NC_000913/2296970‑2297237
                                                                                                                                          |                                                                                                                                   
cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACTCCCTGCGGCCCTCGATTGAGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGGGGAGCGGCTGGTGGGTAGTCAGAATCACAATcc                                                                                                                                   <  2:281154/140‑1 (MQ=255)
cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcc                                                                                                                                   <  2:1539628/140‑1 (MQ=255)
cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcc                                                                                                                                   <  1:1802489/140‑1 (MQ=255)
cgcTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcc                                                                                                                                   <  2:1539629/140‑1 (MQ=255)
     tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATccccccc                                                                                                                             <  1:921065/140‑1 (MQ=255)
     tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATccccccc                                                                                                                             <  2:291268/140‑1 (MQ=255)
     tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATccccccc                                                                                                                             <  1:799106/140‑1 (MQ=255)
     tGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATccccccc                                                                                                                             <  2:11508/140‑1 (MQ=255)
      ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcccccccc                                                                                                                            <  2:123958/140‑2 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGCACATCCTGCGGCCCTCGTTTGCGTNAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGNGTAGTCAGAATCACAATcccccncct                                                                                                                           <  2:118260/140‑3 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACCTCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGCGCGGCTGGTGGGTAGTCAGAATCACAATcccccccct                                                                                                                           <  2:118016/140‑3 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcccccccct                                                                                                                           <  2:817814/140‑3 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcccccccct                                                                                                                           <  1:736783/140‑3 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcccccccct                                                                                                                           <  2:691189/140‑3 (MQ=255)
       gCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcccccccct                                                                                                                           <  1:278577/140‑3 (MQ=255)
          aaaCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcccccccctgct                                                                                                                        >  2:32667/1‑135 (MQ=255)
          aaaCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATcccccccctgct                                                                                                                        >  2:679946/1‑135 (MQ=255)
            aCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCTGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTcc                                                                                                                       <  2:577887/140‑1 (MQ=255)
            aCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCTGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTcc                                                                                                                       <  2:580299/140‑1 (MQ=255)
            aCGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTcc                                                                                                                       <  2:806760/140‑1 (MQ=255)
                 acacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtg                                                                                                                  <  1:236239/140‑1 (MQ=255)
                 acacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtg                                                                                                                  <  1:741870/140‑1 (MQ=255)
                  cacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtgc                                                                                                                 >  2:1170722/1‑140 (MQ=255)
                   acGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtgct                                                                                                                <  1:858875/140‑1 (MQ=255)
                   acGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtgct                                                                                                                <  1:824287/140‑1 (MQ=255)
                   acGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtgct                                                                                                                <  2:1780340/140‑1 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                            >  2:1738321/1‑140 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                            >  1:1179041/1‑140 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                            >  2:1738383/1‑140 (MQ=255)
                       aGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTgcgc                                                                                                            >  2:68921/1‑140 (MQ=255)
                        gCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGcc                                                                                                           <  2:305885/140‑1 (MQ=255)
                         cTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCa                                                                                                          >  1:1139249/1‑140 (MQ=255)
                         cTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCa                                                                                                          >  1:1070188/1‑140 (MQ=255)
                         cTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCa                                                                                                          >  1:342619/1‑140 (MQ=255)
                         cTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCa                                                                                                          >  1:1828000/1‑140 (MQ=255)
                          tCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATa                                                                                                        >  2:727252/1‑140 (MQ=255)
                          tCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATa                                                                                                        >  2:727253/1‑140 (MQ=255)
                             aGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACg                                                                                                      <  1:486282/140‑1 (MQ=255)
                             aGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACg                                                                                                      <  1:423933/140‑1 (MQ=255)
                             aGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACg                                                                                                      <  2:595748/140‑1 (MQ=255)
                              gACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGc                                                                                                     >  1:230141/1‑140 (MQ=255)
                                 ggAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTgg                                                                                                  >  1:462480/1‑140 (MQ=255)
                                 ggAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTgg                                                                                                  >  1:175905/1‑140 (MQ=255)
                                 ggAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTgg                                                                                                  >  1:462300/1‑140 (MQ=255)
                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCTGTATCTCAATCCCCCCCCTGCTCCGTCTGCTGCGCCATACGCTGGGt                                                                                                >  2:1905060/1‑140 (MQ=255)
                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGt                                                                                                >  1:1026938/1‑140 (MQ=255)
                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGt                                                                                                >  1:825042/1‑140 (MQ=255)
                                   aaaaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                               <  2:1036771/140‑1 (MQ=255)
                                       tGCGCCTGAACTTCATGCGGCCCTCGTTTGCGTCTGGGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACACTCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGa                                                                                            <  2:1601987/140‑1 (MQ=255)
                                       tGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGa                                                                                            <  2:1460846/140‑1 (MQ=255)
                                          gcCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGa                                                                                         >  2:1085101/1‑140 (MQ=255)
                                              gAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGa                                                                                     <  2:1918833/140‑1 (MQ=255)
                                              gAACATCATGCGGCCCTCGATTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGa                                                                                     <  2:825979/140‑1 (MQ=255)
                                                aCATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                                                   <  2:156205/140‑1 (MQ=255)
                                                aCATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                                                   <  1:38577/140‑1 (MQ=255)
                                                     atGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGtt                                                                              <  1:401911/140‑1 (MQ=255)
                                                     atGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGtt                                                                              <  1:401728/140‑1 (MQ=255)
                                                            ccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCa                                                                       >  1:258215/1‑140 (MQ=255)
                                                             cTCGTTTGCGTCAGTGAAATGCGGCGAATTTTTCTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCaa                                                                      >  2:1596747/1‑140 (MQ=255)
                                                             cTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCaa                                                                      >  2:210322/1‑140 (MQ=255)
                                                                gTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATcg                                                                   <  1:1572612/140‑1 (MQ=255)
                                                                     cGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTa                                                              >  2:386771/1‑140 (MQ=255)
                                                                     cGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTa                                                              >  1:630878/1‑140 (MQ=255)
                                                                     cGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTa                                                              >  1:775325/1‑140 (MQ=255)
                                                                     cGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACg                                                                                                      >  2:926127/1‑99 (MQ=255)
                                                                     cGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACg                                                                                                      <  1:926127/99‑1 (MQ=255)
                                                                      gTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTaa                                                             <  2:577252/140‑1 (MQ=255)
                                                                          gTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAgg                                                         <  2:109419/140‑1 (MQ=255)
                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                  <  2:862820/140‑1 (MQ=255)
                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                  <  2:1320700/140‑1 (MQ=255)
                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                  <  1:35746/140‑1 (MQ=255)
                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                  <  1:604596/140‑1 (MQ=255)
                                                                                 gcggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                  <  1:604609/140‑1 (MQ=255)
                                                                                   ggcgATTTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCTTACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                <  2:84277/140‑1 (MQ=255)
                                                                                   ggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                <  1:131343/140‑1 (MQ=255)
                                                                                   ggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                <  2:218383/140‑1 (MQ=255)
                                                                                   ggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAgg                                               <  2:1421513/140‑1 (MQ=255)
                                                                                    gcgATTTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTAAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAgg                                               <  1:983487/140‑1 (MQ=255)
                                                                                    gcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAgg                                               <  1:983800/140‑1 (MQ=255)
                                                                                     cgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGTATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGa                                              >  1:1027379/1‑140 (MQ=255)
                                                                                     cgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTATCGTCAATCGCGGTAATAGGCTCGTCGAGGa                                              >  2:172782/1‑140 (MQ=255)
                                                                                     cgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGa                                              >  1:1026682/1‑140 (MQ=255)
                                                                                            tACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATaa                                       <  1:1283501/140‑1 (MQ=255)
                                                                                            tACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATaa                                       <  1:862002/140‑1 (MQ=255)
                                                                                             aCTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACg                                     <  2:182002/140‑1 (MQ=255)
                                                                                              cTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACg                                     <  2:1070188/140‑1 (MQ=255)
                                                                                                  gaGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCGCTGCTCCGTATGCTGCGCCGTACGCTGGGTCAGACGATCGACACCGTTATCGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGc                                 <  2:1005179/139‑1 (MQ=255)
                                                                                                    gCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCAc                               <  2:1098011/140‑1 (MQ=255)
                                                                                                    gCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCAc                               <  1:122250/140‑1 (MQ=255)
                                                                                                        cGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGt                           <  2:1769032/140‑1 (MQ=255)
                                                                                                        cGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGt                           <  1:1561532/140‑1 (MQ=255)
                                                                                                        cGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGt                           <  2:680611/140‑1 (MQ=255)
                                                                                                         gTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTc                          <  2:1514095/140‑1 (MQ=255)
                                                                                                             aGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGcc                      >  1:1716569/1‑140 (MQ=255)
                                                                                                                  cTGGTGGGTTGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCATTCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCTCGGGTCAGCCACAGa                 <  1:233249/140‑1 (MQ=255)
                                                                                                                  cTGGTGGGTAGTCAGAATCACCATCCCCCCCCTGCTGCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATTCATAACGTGGCACGGGTCAGCCACAGa                 <  2:105390/140‑1 (MQ=255)
                                                                                                                            gTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAg       >  1:722701/1‑140 (MQ=255)
                                                                                                                               aGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGa    >  1:1490877/1‑140 (MQ=255)
                                                                                                                               aGAATCACAATCCCCCCCCTGCTCCGTAAGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGa    >  1:1336538/1‑140 (MQ=255)
                                                                                                                                 aaTCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcg  <  1:112926/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                   
CGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACG  >  NC_000913/2296970‑2297237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: