Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C98.9% 324.1 / ‑2.5 89intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (45/43);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*NC_0009134,296,3802.G97.8% 318.3 / 1.5 89intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (43/43);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAC  >  NC_000913/4296243‑4296505
                                                                                                                                           ||                                                                                                                               
tatagTAGGTCGCACAAGGCGTTTATGCCGCAACCGAAATCAAAGACTGATGCGACTCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTAAGCGAGAATTTGTAGGTCGGAT‑AAGGCGTTTA‑‑c‑c                                                                                                                              <  2:167462/136‑1 (MQ=11)
                                                                   cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                                                            >  2:36463/1‑140 (MQ=255)
                                                                    ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                                                           >  1:448817/1‑140 (MQ=255)
                                                                     tATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑TAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGACTTATCAGGCCTATCTTAACCGTTGGTTa                                                          <  1:260724/140‑1 (MQ=11)
                                                                     tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTTGCGTGATTTTGTCGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGTCATGATTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                          <  1:1423000/140‑1 (MQ=11)
                                                                     tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                          <  1:1347912/140‑1 (MQ=255)
                                                                     tATCAGGACTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACTTCTATGCCTGATGCGACGCTTGCCGCGTCTTATAAGGCCTATCTTAACCGTTGGTTa                                                          <  1:182808/140‑1 (MQ=11)
                                                                      aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                         >  2:1320643/1‑140 (MQ=255)
                                                                      aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGACGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGAGGCGACGCTTGCCGCGTCTGATCAGGCCTATCTTAACCGTTGGTTaa                                                         >  2:1320096/1‑140 (MQ=11)
                                                                           gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                    >  2:480201/1‑140 (MQ=255)
                                                                             cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCa                                                  >  2:326120/1‑140 (MQ=255)
                                                                             cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCa                                                  >  2:327519/1‑140 (MQ=255)
                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑TCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                <  2:913259/140‑1 (MQ=255)
                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaat                                                >  2:628762/1‑139 (MQ=255)
                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                <  1:53109/140‑1 (MQ=255)
                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                <  1:1069426/140‑1 (MQ=255)
                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                <  2:1593341/140‑1 (MQ=255)
                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                <  1:1218587/140‑1 (MQ=255)
                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                <  1:1354205/140‑1 (MQ=255)
                                                                                cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                               >  2:1846681/1‑140 (MQ=255)
                                                                                cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGACGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGACTTATCAGGCCTATCTTAACCGTAGGTTAATTTTTCAAAc                                               >  1:1773735/1‑140 (MQ=14)
                                                                                    aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt                                           >  2:260044/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAACTAAACCGTTGGATAATTTTTCAAACTCTTc                                          >  1:1637604/1‑140 (MQ=14)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTTACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:741590/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:1632734/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:1693800/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:1632495/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:50535/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:1587736/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  2:1001421/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:670364/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:766232/1‑140 (MQ=255)
                                                                                     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  2:366171/1‑140 (MQ=255)
                                                                                          cgcATTAGGCTGATTTAGCGTGATTGTGTTGGTCGGAA‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTGGGTTAATTTTTCAAACTCGTCACTcc                                     >  2:419033/1‑140 (MQ=14)
                                                                                          cgcAATAGCCTTATTTAGCGTGATTTTGTAGGTCTGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAACAGGCCTATATTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  1:874992/140‑1 (MQ=17)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     >  1:298739/1‑140 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  1:1794156/140‑1 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     >  1:514246/1‑140 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     >  2:426740/1‑140 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     >  2:571653/1‑140 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  1:875512/140‑1 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  2:710460/140‑1 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     >  1:1096369/1‑140 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  1:840076/140‑1 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  1:840085/140‑1 (MQ=255)
                                                                                          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTAATAATCTTCacaca                                     >  2:1069426/1‑139 (MQ=14)
                                                                                            cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg                                   <  1:1277430/140‑1 (MQ=255)
                                                                                             aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCgg                                  >  1:1016067/1‑140 (MQ=255)
                                                                                             aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCgg                                  >  1:1333904/1‑140 (MQ=255)
                                                                                               tAGCCTGATTTAGCGTGATTTTGTAGGTAGGAT‑AAGGCGTTTACTC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa                                >  2:959466/1‑140 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCTTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  2:1813120/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaat                           >  1:302023/1‑139 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:691151/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  2:801703/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:1096785/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  2:576009/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:209918/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  2:204088/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  2:1321435/140‑1 (MQ=255)
                                                                                                    tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           >  1:287005/1‑140 (MQ=255)
                                                                                                     gATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                          >  2:486713/1‑140 (MQ=255)
                                                                                                     gATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGAGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                          >  2:959683/1‑140 (MQ=255)
                                                                                                      aTTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                         >  2:4478/1‑140 (MQ=255)
                                                                                                      aTTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTAAACCGTTGGTTAATTTTTAAAACTCTTCTCACCCGGTATCAAAcc                         >  2:4670/1‑140 (MQ=18)
                                                                                                       tttAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                        >  1:982685/1‑140 (MQ=255)
                                                                                                       tttAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                        >  1:982416/1‑140 (MQ=255)
                                                                                                         tAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                      <  1:455986/140‑1 (MQ=255)
                                                                                                            cGTGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa                   >  1:1729131/1‑140 (MQ=255)
                                                                                                              tGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                 <  1:197490/140‑1 (MQ=255)
                                                                                                              tGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                 <  1:466882/140‑1 (MQ=255)
                                                                                                              tGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                 <  1:1544123/140‑1 (MQ=255)
                                                                                                              tGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                 <  1:1544476/140‑1 (MQ=255)
                                                                                                              tGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                 <  1:466877/140‑1 (MQ=255)
                                                                                                              tGATTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa                 <  1:923679/140‑1 (MQ=255)
                                                                                                                aTTTTGTAGGTCGGATAAAGCCGTTAAC‑C‑C‑GCAACCGAAAACAAATCCAGATTCGACGCATGCCCCCACTTATAAGGCCAAAATTAACCGTTGGGTAATTTTTCAAAAAAGTCAAACCCGGTATCAAAACCTTCCaaaaa               >  2:1428968/1‑136 (MQ=255)
                                                                                                                aTTTTGTAGGTCGGAT‑AAGTCGTTTACGC‑C‑GCATCCGACATCAATGACAGAGGCGACCCTTGCCGCGTCATATCATGCCTATCATAACCGTTGGTTAATTTTTAAATCTCTTCACACACGGTATCAAACCCTTCCATACa               >  2:1428958/1‑140 (MQ=18)
                                                                                                                aTTTTGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTTGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa               >  2:1649627/1‑140 (MQ=255)
                                                                                                                   ttGTAGTTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  1:914430/140‑1 (MQ=255)
                                                                                                                   ttGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  1:1533632/140‑1 (MQ=255)
                                                                                                                   ttGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  1:914479/140‑1 (MQ=255)
                                                                                                                   ttGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  1:153027/140‑1 (MQ=255)
                                                                                                                   ttGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  1:154513/140‑1 (MQ=255)
                                                                                                                   ttGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  2:298739/140‑1 (MQ=255)
                                                                                                                   ttGTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  1:1479497/140‑1 (MQ=255)
                                                                                                                     gTAGGTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa          <  1:683369/140‑1 (MQ=255)
                                                                                                                        ggTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt       <  2:1824263/140‑1 (MQ=255)
                                                                                                                        ggTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt       <  1:1001421/140‑1 (MQ=255)
                                                                                                                        ggTCGGAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTAGCCTCGTCTTAACAGGCCTATCTTACCCGTTGGTAAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt       <  2:802549/140‑1 (MQ=25)
                                                                                                                        ggTCGGAT‑AAGGCGTTTA‑‑CGCTGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt       <  2:1823681/140‑1 (MQ=255)
                                                                                                                             gAT‑AAGGCGTTTACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc  >  1:338269/1‑140 (MQ=255)
                                                                                                                                           ||                                                                                                                               
TTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTTA‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAC  >  NC_000913/4296243‑4296505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑