Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 737,928 C→G 6.4% pseudogene (1104/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913737,9280CG 6.4% 562.8 / 24.9 155pseudogene (1104/1137 nt)ybfLpseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
Reads supporting (aligned to +/- strand):  ref base C (63/82);  new base G (7/3);  total (70/85)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

CAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACT  >  NC_000913/737790‑738067
                                                                                                                                          |                                                                                                                                           
cAAAAAAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTcg                                                                                                                                            >  1:190903/1‑140 (MQ=255)
    aTACGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTc                                                                                                                                        <  1:1326166/140‑1 (MQ=255)
    aTAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTc                                                                                                                                        <  1:1326229/140‑1 (MQ=255)
     tAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCa                                                                                                                                       >  2:1398295/1‑140 (MQ=255)
     tAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCa                                                                                                                                       <  2:714902/140‑1 (MQ=255)
      aagcagAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAg                                                                                                                                      <  2:1205604/140‑1 (MQ=255)
      aagaagAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAg                                                                                                                                      <  1:1673301/140‑1 (MQ=255)
      aagaagAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAg                                                                                                                                      <  1:1300/140‑1 (MQ=255)
      aagaagAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAg                                                                                                                                      <  1:971116/140‑1 (MQ=21)
              gAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCg                                                                                                                              <  2:357772/140‑1 (MQ=255)
              gAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCg                                                                                                                              <  2:211377/140‑1 (MQ=255)
                aaCGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCggg                                                                                                                            >  2:1706486/1‑140 (MQ=255)
                  cgccgcAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGa                                                                                                                          >  2:1797948/1‑140 (MQ=255)
                       cAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCggg                                                                                                                     <  1:1236136/140‑1 (MQ=40)
                       cAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCggg                                                                                                                     <  2:70487/140‑1 (MQ=40)
                       cAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCggg                                                                                                                     <  2:548299/140‑1 (MQ=40)
                       cAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCggg                                                                                                                     <  1:236360/140‑1 (MQ=40)
                       cAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCggg                                                                                                                     <  2:1626422/140‑1 (MQ=40)
                       cAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCggg                                                                                                                     <  2:327169/140‑1 (MQ=40)
                       cAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCggg                                                                                                                     <  2:327011/140‑1 (MQ=40)
                         gAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCt                                                                                                                   >  1:140354/1‑140 (MQ=40)
                             tataTTCAGGGATACGGCACATAGCTATAAATATTTTGACGCATGATACGGTATTCAACGCAGGGTTAAGCCGTAAGATGCGAACAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg                                                                                                               <  2:1887718/140‑1 (MQ=11)
                                  tCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATc                                                                                                          <  2:664245/140‑1 (MQ=40)
                                    aGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCtt                                                                                                        >  2:133567/1‑140 (MQ=40)
                                         tACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCt                                                                                                   >  1:644259/1‑140 (MQ=255)
                                         tACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCt                                                                                                   <  2:1458296/140‑1 (MQ=255)
                                              cacaTCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCttt                                                                                              <  1:411520/140‑1 (MQ=255)
                                              cacaTCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCttt                                                                                              <  1:703289/140‑1 (MQ=255)
                                                 aTCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTg                                                                                           >  2:863891/1‑140 (MQ=255)
                                                 aTCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTg                                                                                           <  2:919749/140‑1 (MQ=255)
                                                  tCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGt                                                                                          >  1:909607/1‑140 (MQ=255)
                                                  tCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGt                                                                                          <  2:508889/140‑1 (MQ=255)
                                                     cTATTAATATTTTAACGAATGATACGGTATTCATGGCAGGTTTAAGACGTAAGCTGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGATCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACt                                                                                       <  2:1769113/140‑1 (MQ=17)
                                                      tATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTa                                                                                      >  1:873681/1‑140 (MQ=255)
                                                        ttAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTaaa                                                                                    >  1:657549/1‑140 (MQ=255)
                                                           atatTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGACCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAaataa                                                                                 >  2:5027/1‑140 (MQ=255)
                                                               tttAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCg                                                                             >  1:1894455/1‑140 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGGAGCCATGGATAGAAACTATCTCGCGTCAGTCATTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTaaaaa                                                            <  2:1614040/140‑1 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGTATAGAAACTATCTCGCTTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATTTTTaaaaa                                                            <  2:1613993/140‑1 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTaaaaa                                                            <  2:1819024/140‑1 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTaaaaa                                                            <  2:1819416/140‑1 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTaaaaa                                                            <  2:1223392/140‑1 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTaaaaa                                                            <  2:1644227/140‑1 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTAGCATTTTATGTTTaaaaa                                                            <  2:1138627/140‑1 (MQ=255)
                                                                                tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAAATATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTTTGTTTaaaaa                                                            <  2:85244/140‑1 (MQ=255)
                                                                                 aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAAt                                                           <  2:1560181/140‑1 (MQ=255)
                                                                                 aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAAt                                                           <  2:1558971/140‑1 (MQ=255)
                                                                                 aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAAt                                                           <  2:1657389/140‑1 (MQ=255)
                                                                                 aTTCAAGGAAGGGTTAAGACGTAAGATGCGAAAAGCAGCCTTGGATAGAAACTATCTCGCGTCAGTCATTGTGGGGAGCGGGATTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATGCGCATTTTATGTTTAAAAAt                                                           <  2:1723961/140‑1 (MQ=21)
                                                                                  ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTATAAAtt                                                          >  1:1608451/1‑140 (MQ=255)
                                                                                   tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTg                                                         >  2:1847791/1‑140 (MQ=255)
                                                                                       ggCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGat                                                     >  2:1653711/1‑140 (MQ=255)
                                                                                         cAGGGTTTCGACGTAAGCGGCGAATAGCAGCCATGGATAGAAACTCTCTCGCGTCTGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGatat                                                   <  2:1793077/140‑1 (MQ=25)
                                                                                         cAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCTTTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGatat                                                   <  2:1578411/140‑1 (MQ=255)
                                                                                         cAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAACAATTCGCATTTTATGTTTAAAAATTGAGatat                                                   >  1:1168635/1‑140 (MQ=255)
                                                                                          aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTTAAAATTGAGATCtt                                                  >  1:1015796/1‑140 (MQ=255)
                                                                                          aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATAtt                                                  >  1:1005161/1‑140 (MQ=255)
                                                                                          aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATAtt                                                  >  1:1005487/1‑140 (MQ=255)
                                                                                               tAAGACGTTAGATGCGTAAAGCAGCCTTGGATTGTAACTTTGTCGCGTCAGTACTTGGGTGGAGCGGGCTTTCGTAATCTTTCGCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCtta                                             <  2:291001/140‑1 (MQ=25)
                                                                                               tAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCtta                                             <  2:929820/140‑1 (MQ=255)
                                                                                               tAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCtta                                             <  2:930532/140‑1 (MQ=255)
                                                                                               tAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCtta                                             <  2:1324973/140‑1 (MQ=255)
                                                                                                 agtcttAAGATGCGTATAGCAGCCTTGGATAGTTTCTATCTCGCGTGAGTCCTTGCGGGGTGCGGGCTTTCGTATTCTTTCCGTGCTTTTTGTTCTAAATTTTTTGCTTTTTATGTTTAAAAATTGAGTTATTCattatt                                           <  1:1467585/135‑1 (MQ=255)
                                                                                                 aGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCttatt                                           <  1:1467594/140‑1 (MQ=255)
                                                                                                 aGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCttatt                                           <  1:1467252/140‑1 (MQ=255)
                                                                                                      tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTg                                      >  2:782230/1‑140 (MQ=255)
                                                                                                      tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTg                                      >  1:1607961/1‑140 (MQ=255)
                                                                                                      tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTg                                      >  2:821496/1‑140 (MQ=255)
                                                                                                      tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTg                                      >  2:83362/1‑140 (MQ=255)
                                                                                                      tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTg                                      >  1:1362944/1‑140 (MQ=255)
                                                                                                      tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTg                                      >  2:197492/1‑140 (MQ=255)
                                                                                                      tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTg                                      >  1:643529/1‑140 (MQ=255)
                                                                                                       aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGa                                     >  1:518587/1‑140 (MQ=255)
                                                                                                       aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGa                                     >  1:1489675/1‑140 (MQ=255)
                                                                                                       aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGa                                     >  1:729799/1‑140 (MQ=255)
                                                                                                       aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGa                                     >  1:1022268/1‑140 (MQ=255)
                                                                                                       aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAACAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCtaa                                     >  1:626451/1‑138 (MQ=255)
                                                                                                        aGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGaa                                    >  1:450933/1‑140 (MQ=255)
                                                                                                         gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAg                                   <  2:754752/140‑1 (MQ=255)
                                                                                                         gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAg                                   <  2:529769/140‑1 (MQ=255)
                                                                                                         gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAg                                   <  2:754927/140‑1 (MQ=255)
                                                                                                             cGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGt                               >  2:1576671/1‑140 (MQ=255)
                                                                                                                   gcagcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTa                         <  1:1605632/140‑1 (MQ=255)
                                                                                                                   gcagcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTa                         <  1:1885447/140‑1 (MQ=255)
                                                                                                                   gcagcCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTa                         <  1:289916/140‑1 (MQ=255)
                                                                                                                       ccATGTATAGAAACTTTCTCGATTCAGTCCTTGCTTGGAGAGGGATTTAGTAATCTTTCCCTGATTTTTGTAATAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGc                     <  1:1757699/140‑1 (MQ=18)
                                                                                                                       ccAAGGATTGTAACCTTCTCGATTCAGTCCGTTGGTGGCGAGGGCATTCTTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGTGATATTCCTTATTACCTGAAGCTGTTTTTTATTGc                     <  1:1757677/140‑1 (MQ=14)
                                                                                                                         aTGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCtt                   >  1:548736/1‑140 (MQ=255)
                                                                                                                              tAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACa              <  1:1530501/140‑1 (MQ=255)
                                                                                                                              tAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACa              <  1:113095/140‑1 (MQ=255)
                                                                                                                              tAGAAACTATATCGCGTCATTACTTGCGGTGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACa              <  2:802898/140‑1 (MQ=255)
                                                                                                                                 aaaCTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGa           >  1:1086921/1‑140 (MQ=255)
                                                                                                                                 aaaCTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGa           >  2:1430392/1‑140 (MQ=255)
                                                                                                                                 aaaCTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGa           >  2:288834/1‑140 (MQ=255)
                                                                                                                                     tATCTCGCGTCATTCCTTTAGGGTAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTTTACTAAATAATTAGCTTTTTATGTTTAAAAATTGAGATATTACTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCaa       <  2:1131997/140‑1 (MQ=18)
                                                                                                                                      aTCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCaaa      >  1:1632267/1‑140 (MQ=255)
                                                                                                                                      aTCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCaaa      >  1:618014/1‑140 (MQ=255)
                                                                                                                                       tctcGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAAt     >  1:1105505/1‑140 (MQ=255)
                                                                                                                                       tctcGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTAGTACTAAATAATTCGAATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAAt     >  1:167503/1‑140 (MQ=255)
                                                                                                                                          cgcgTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATAct  <  2:1263467/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAATCTTTCCCTGCTTTTTGTACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACT  >  NC_000913/737790‑738067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: