Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,226 T→G 11.8% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2260TG11.8% 247.5 / 6.1 146intergenic (‑369/+126)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (89/31);  new base G (5/14);  total (95/51)
Fisher's exact test for biased strand distribution p-value = 8.72e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTT  >  NC_000913/915088‑915364
                                                                                                                                           |                                                                                                                                          
  tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcg                                                                                                                                         >  1:1488756/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgc                                                                                                                                        >  1:136611/1‑140 (MQ=17)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcg                                                                                                                                       >  1:494645/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcg                                                                                                                                       >  1:128209/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcg                                                                                                                                       >  1:208213/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcg                                                                                                                                       >  1:1873414/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcg                                                                                                                                       >  2:915339/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcg                                                                                                                                       >  2:83537/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgcg                                                                                                                                       >  1:819122/1‑140 (MQ=32)
    aGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGGGGCCGCCCCCTgcgcg                                                                                                                                       >  1:11732/1‑140 (MQ=17)
       gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTc                                                                                                                                    >  2:718669/1‑140 (MQ=32)
          cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtt                                                                                                                                 >  2:820989/1‑140 (MQ=32)
          cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtt                                                                                                                                 >  1:94943/1‑140 (MQ=32)
          cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtt                                                                                                                                 >  1:1489406/1‑140 (MQ=32)
          cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtt                                                                                                                                 >  1:358113/1‑140 (MQ=32)
          cgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtt                                                                                                                                 >  1:95013/1‑140 (MQ=32)
                accgggATGTTACCTCGCGCCCGACCGGGTAGCCTTATGGGATAAGTGGTGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCTGGGGGGGGCGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCa                                                                                                                           <  2:1829430/137‑1 (MQ=11)
                 cAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                          >  2:823589/1‑140 (MQ=32)
                    ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTgg                                                                                                                       >  2:939896/1‑140 (MQ=33)
                      ttGTTACCTCGCGCCCGACCCGGTTGCCTTATGGTATATGTCGTGGGCACCGCGCTGCGGCTTTTTTGTTCGCCAGCGCCGGGGGGTGCTGGGGGGGGGGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                     <  2:1762845/139‑1 (MQ=11)
                      aTGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGAGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                     >  2:1747493/1‑140 (MQ=21)
                       tGTTACCTCGCGCCCGACCCGGTCGCGGTTGGGGCGGGGTCGGGGGCGCCGCGCGGCGGCGTGTGGGTTCGCGAGAGCGCGGGGGTGCGGGGGGCGGCGGCGATTGGCGGCCCGCGGCGCGCTCCGTGCGCCGGTGGctg                                                                                                                    >  2:698840/1‑138 (MQ=2)
                       tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCaa                                                                                                                    >  2:298422/1‑140 (MQ=35)
                       tGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAGAAGGCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCACCCCCTGCGCGCTCCTTTCGCCAGTGGCaa                                                                                                                    >  1:777597/1‑140 (MQ=255)
                        gTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAat                                                                                                                   >  2:1638678/1‑140 (MQ=37)
                         ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAata                                                                                                                  >  2:78124/1‑140 (MQ=37)
                         ttACCTCGCCCCCTACCGGTTAGCCTTATGGATTATGTCGTGTCCACGGGCCTGCGGGTATTTTTTTCGACTGTGCCGGGGGGTGCTGGGGGCGGGGCCTTTTGGCGGCCCGCTGCGCGCTCCTTGCGCCAGTGGCAata                                                                                                                  <  2:1568514/140‑1 (MQ=2)
                         ttACCACGCGCCCGACCAGGTATCCGTAAGTGATAAGTCGAGGGAACCGCGCAGAGGCGATTATGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTTTCAGCCCCCTGCGAGATCCTTGCGCCGGAGGCAata                                                                                                                  >  2:1444305/1‑140 (MQ=11)
                               cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGc                                                                                                            >  2:978727/1‑140 (MQ=255)
                               cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGc                                                                                                            >  2:978448/1‑140 (MQ=255)
                                gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCCGTGGCAATATGTTGCt                                                                                                           >  2:1219068/1‑140 (MQ=255)
                                gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                                           >  2:486769/1‑140 (MQ=255)
                                gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCt                                                                                                           >  2:813878/1‑140 (MQ=255)
                                 cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCtt                                                                                                          >  1:1736908/1‑140 (MQ=255)
                                 cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCtt                                                                                                          >  1:912183/1‑140 (MQ=255)
                                   ccggACCGGGTTGCCGTAAGGTATATGTCGGGGGCACCGCGCTGGGGCGTTTTTGTTCGCATGCGCCGGGGGGTGCTGGGGGGGGGGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg                                                                                                        <  2:1852599/137‑1 (MQ=14)
                                       aCCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                    >  2:726946/1‑140 (MQ=255)
                                       aCCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                    >  1:497557/1‑140 (MQ=255)
                                                   tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAgcgc                                                                                        >  1:743011/1‑140 (MQ=255)
                                                   tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAAAATGTTGCTTAGCTCATGAAAGGAgcgc                                                                                        >  1:910677/1‑140 (MQ=255)
                                                     aGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTATCTCATGAAAGGAGCGcaa                                                                                      >  2:224859/1‑140 (MQ=255)
                                                      gggATACGTGGGGGGCACCGCGCAGGGGCGTTTGTGTTCGCCAGTGCCCGGGGTGGCTGGGGGCGGGGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATCTGTTTCTTAGCTCATGTAAGGAGCGcaac                                                                                     <  2:1484153/140‑1 (MQ=17)
                                                          tAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGATCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAga                                                                                 >  2:1519360/1‑140 (MQ=255)
                                                           aaGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgat                                                                                >  2:1403100/1‑140 (MQ=255)
                                                            aGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatg                                                                               >  2:1344589/1‑140 (MQ=255)
                                                                 aGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGaa                                                                          >  1:670041/1‑140 (MQ=255)
                                                                 aGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCCGTGGCAATATGTTGCGTAGCTCATGAAAGGAGCGCAACAACATGATGaa                                                                          >  1:1571074/1‑140 (MQ=255)
                                                                  gggCCCGGCGCGGCGGCTTTTTTGTTGGCCAGCGCCGGGGGGTGCTGGGGGGGGCGGCGTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  2:542063/140‑1 (MQ=18)
                                                                  gggCCCCGCGCTGCGGGTTTTTTGTTCGCATTCGCCCGGGGGGGCGGGGGGGGGGGGCTTGGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGTTGAAt                                                                         <  2:1545416/140‑1 (MQ=16)
                                                                  gggCAGCGCCCCGCGGCTTTTTTTTTCGTATGAGCCGGGGGGTGCTGGGGGCGGGTCGGATGGGCCGCCCCCGGCGCGCTACTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  2:1030123/140‑1 (MQ=255)
                                                                  gggCACGGGGCAGGGGCTTTTTTTTCCGCCTGCGCCCGGGGGTGCCGGGGGGGGCGGCTTTGGCCCGCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  2:416933/140‑1 (MQ=18)
                                                                  gggCACGGCGCCGGGGGGTTTTTGTTCGCCTGAGCCGGGGGGTGCTGGGGGGGGCGGCGTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  2:1544657/140‑1 (MQ=18)
                                                                  gggCACCGGGCTGGGGTTTTTTTTTTCGCCTTAGCCGGGGGGTGCTGGGGGCGGCGGTTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  2:1375411/140‑1 (MQ=18)
                                                                  gggCACCGGGCAGCGGCTTTTTTGTTCGCCTTAGCCCGGGGGTGCTGGGGGCGGCGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  2:803836/140‑1 (MQ=18)
                                                                  gggCACCGCGCCGGGGTGTTTTTTTCCGCAAGTCCGGGGGGGGGCTGGGGGGGCCGGGGTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  1:1759636/140‑1 (MQ=16)
                                                                  gggCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCATAGCTCAAGTAAGGAGCGCAACAAGATGATGAAt                                                                         >  1:238601/1‑140 (MQ=255)
                                                                  gggCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCGTAGCTCAGGCAAGGAGCGCACCAAGAtgatgaca                                                                         >  2:946441/1‑138 (MQ=18)
                                                                  ggcccccGCGCTGGGGCTTTTTTGTCCGCCTGCGCGGGGGGGTGGGGGGGGGGGGGGGTTTGGGCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAt                                                                         <  1:1638678/135‑1 (MQ=16)
                                                                     ccccGCGCTGGGGCTTTTTTGTACGCCTGTGCCGGGGGTGGCTGGGGGGGGCGGGTTTTGCCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCaa                                                                      <  2:1322874/138‑1 (MQ=18)
                                                                        cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                   >  2:1580728/1‑140 (MQ=255)
                                                                        cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                   >  2:589989/1‑140 (MQ=255)
                                                                        cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                   >  2:588812/1‑140 (MQ=255)
                                                                        cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                   >  2:561006/1‑140 (MQ=255)
                                                                        cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                   >  1:587130/1‑140 (MQ=255)
                                                                        cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                   >  2:560337/1‑140 (MQ=255)
                                                                        cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGAGCAGGGGGCCGCCGCGCTTGGCCGCCCCCTGCGCGCTCCATGCGCCAGGGGCAATATGTTGCTTAGCTCCTGAAAGGAGCGCCACAAGAAGAGGAaacaaccg                                                                   >  2:425992/1‑138 (MQ=18)
                                                                        cgcgCAGCGGCGAGTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGTTTGGCCGCGCCCTGCGCGCTCCTTGCGCCCGTGGCGTTATGGTGCGTAGCTCATGTCAGGTGCGCAACAAGGTGATGAAtgatctg                                                                   >  2:921459/1‑134 (MQ=17)
                                                                          cgcAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACatat                                                                 >  2:466353/1‑140 (MQ=255)
                                                                            cAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaa                                                               >  2:773288/1‑140 (MQ=255)
                                                                             aGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaac                                                              >  2:415938/1‑140 (MQ=255)
                                                                                    atgttGTTCGCCCGAGCCGGGGGGTCCAGGGGGCGGCGGCGCTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATCTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACCTATAACAACATCt                                                       <  2:1691534/137‑1 (MQ=25)
                                                                                    aTTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCt                                                       >  2:771332/1‑140 (MQ=255)
                                                                                            cGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaaa                                               >  2:1050962/1‑140 (MQ=255)
                                                                                                tgcgcggggggggGCTGGGGGGGGGGGCTTTTGGCCCCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                           <  1:737413/133‑1 (MQ=37)
                                                                                                tgcgcgggggggTGCGGGGGGGGGGGGTTTTGGGCCCCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                           <  1:1125822/133‑1 (MQ=37)
                                                                                                tgcgCCGGGGGGTGCTGGGGGCGGGGGCGTTTGCCCCCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                           <  2:340668/137‑1 (MQ=37)
                                                                                                cgcgcggggggggGCGGGGGGGGGGGGCTCTGGGCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                           <  1:88589/133‑1 (MQ=37)
                                                                                                cgcgCCGGGGGGTGCTGGGGGCGGGGGCTTTTGGCCCCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                           <  1:688534/137‑1 (MQ=37)
                                                                                                 gtgCCGGGGGGTGCTGGGGGGGGGGGCTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt                                          <  1:1755066/138‑1 (MQ=37)
                                                                                                 gcgggggggggTGCTGGGGGGGGGGGGGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCt                                          <  1:915339/134‑1 (MQ=37)
                                                                                                    cccGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTTCGCCAGTGGCAATATTTTGCTTATCTCATGAAAGGAGCGCAACAAGATTATGAATCAACATATAAAAACATCTTAAAAAAAGGCCTGaa                                       >  1:1533414/1‑139 (MQ=37)
                                                                                                     cggggggTGCTGGGGGGGGCGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACa                                      <  1:962684/138‑1 (MQ=255)
                                                                                                     cggggggTGCGGGGGGGGGGGGGTTTTGGCCCCCCCTGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACa                                      <  1:1491421/138‑1 (MQ=37)
                                                                                                         gggTGCTGGGGGGGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTAc                                  <  2:792458/140‑1 (MQ=255)
                                                                                                          gtcggccgggggCCGCTGCCACTCGCCCCCACCTCCGCCCTCCTTGCTCCAGAGGCTCGATTATGCGTATCTCAATACAGCACCGCAACAACCTGAAGAAACAACAAATAACAACCTCTTAAAAAAAGGCCTGACATCAc                                  <  2:1922323/133‑1 (MQ=255)
                                                                                                            gggggggggggggggGCGTTGGGCCGCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGc                                <  1:1607590/135‑1 (MQ=255)
                                                                                                              cAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAg                             >  2:277558/1‑140 (MQ=255)
                                                                                                                  ggggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                          >  2:1819056/1‑140 (MQ=255)
                                                                                                                    gggggcggcgTTTGGCCGCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTGGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCtt                        <  1:706859/137‑1 (MQ=38)
                                                                                                                    ggcggcggcGAATGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCACATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCATAAAAAAAGGCCTGACATTACGCCAGGCCtt                        >  2:86935/1‑140 (MQ=255)
                                                                                                                      cggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCt                      >  1:434059/1‑140 (MQ=255)
                                                                                                                       ggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTg                     >  1:611855/1‑140 (MQ=255)
                                                                                                                       ggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTg                     >  2:1145546/1‑140 (MQ=255)
                                                                                                                       ggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTg                     >  1:512564/1‑140 (MQ=255)
                                                                                                                       ggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGGTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTg                     >  1:514278/1‑140 (MQ=255)
                                                                                                                         cggctATGGGCCGCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCg                   <  1:776852/140‑1 (MQ=255)
                                                                                                                             taTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa               <  2:910677/139‑1 (MQ=255)
                                                                                                                                 ggCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat          >  1:1823795/1‑140 (MQ=255)
                                                                                                                                       ccccCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc     <  2:100728/140‑1 (MQ=255)
                                                                                                                                          cTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCTACAAGCTGATGTATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCttt  <  2:677632/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTT  >  NC_000913/915088‑915364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: