Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,049,827 T→G 5.3% intergenic (‑83/‑6) gfcA ← / → insA O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,049,8270TG 5.3% 315.2 / 9.1 112intergenic (‑83/‑6)gfcA/insAO‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
Reads supporting (aligned to +/- strand):  ref base T (67/39);  new base G (2/4);  total (69/43)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 115 total aligned reads displayed.

CGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTA  >  NC_000913/1049688‑1049966
                                                                                                                                           |                                                                                                                                           
cGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGtt                                                                                                                                             >  1:32191/1‑140 (MQ=255)
       ggCGTCGTCAACATGAAGGCCATATGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTCCTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGt                                                                                                                                      <  2:316223/140‑1 (MQ=255)
        gCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTg                                                                                                                                     >  2:812624/1‑140 (MQ=255)
        gCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGAGTTAGTGTATGATGGTGTGTTTGAGGTTCTCCAGTg                                                                                                                                     >  2:236646/1‑140 (MQ=255)
        gCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACATACTGATTTAGTGTATGATGGTGTTTTTTAGGTTCTCCAGTg                                                                                                                                     >  2:812662/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCACCTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:1669418/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:216706/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:120434/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:935073/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:668284/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:180026/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:532539/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:532532/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:524732/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:164415/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:887540/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:633107/1‑140 (MQ=255)
           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt                                                                                                                                  >  1:1681207/1‑140 (MQ=255)
            cgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCtt                                                                                                                                 <  1:1765498/140‑1 (MQ=255)
             gtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTc                                                                                                                                >  2:23229/1‑140 (MQ=255)
                  cATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTgggt                                                                                                                           >  2:892272/1‑137 (MQ=255)
                  cATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGttt                                                                                                                           >  2:892238/1‑140 (MQ=255)
                    tGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCt                                                                                                                         <  1:652803/140‑1 (MQ=255)
                     gAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTa                                                                                                                        >  1:1178824/1‑140 (MQ=255)
                     gAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTa                                                                                                                        >  1:133609/1‑140 (MQ=255)
                        ggCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCa                                                                                                                     <  2:1218333/140‑1 (MQ=255)
                             tAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGt                                                                                                                >  2:526311/1‑140 (MQ=255)
                             tAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGt                                                                                                                <  1:787574/140‑1 (MQ=255)
                                   ttGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCcctcc                                                                                                          >  2:1713311/1‑140 (MQ=255)
                                    tGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCcctcct                                                                                                         >  2:463828/1‑140 (MQ=255)
                                       aGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGtt                                                                                                      <  1:1830301/140‑1 (MQ=255)
                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATTGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  <  2:1527933/140‑1 (MQ=255)
                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  <  2:794206/140‑1 (MQ=255)
                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  <  2:1720083/140‑1 (MQ=255)
                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  <  2:138126/140‑1 (MQ=255)
                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  <  2:1401383/140‑1 (MQ=255)
                                           aGCTTGTGTTTCATAACTATTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGATGTTATCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  >  2:434976/1‑140 (MQ=255)
                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:711160/140‑1 (MQ=255)
                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:1260881/140‑1 (MQ=255)
                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:532532/140‑1 (MQ=255)
                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:532539/140‑1 (MQ=255)
                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  1:452695/140‑1 (MQ=255)
                                              ttGTGTTTCATAACTTTACCTTTAGTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  1:1896904/140‑1 (MQ=255)
                                                 tgtTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGa                                                                                            <  2:471985/140‑1 (MQ=255)
                                                 tgtTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCACCTGTTCAGCTACTGa                                                                                            <  2:1920307/140‑1 (MQ=255)
                                                   tttCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACg                                                                                          <  1:356382/140‑1 (MQ=255)
                                                      cATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTATCCCTCCTGTTCAGCTACTGACgggg                                                                                       <  1:1395895/140‑1 (MQ=255)
                                                       aTAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGggt                                                                                      >  2:1505114/1‑140 (MQ=255)
                                                       aTAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGggt                                                                                      >  1:1076079/1‑140 (MQ=255)
                                                       aTAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGggt                                                                                      >  1:287588/1‑140 (MQ=255)
                                                                 cTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAAc                                                                            <  1:1444873/140‑1 (MQ=255)
                                                                   ttATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACgg                                                                          >  2:768826/1‑140 (MQ=255)
                                                                      ttCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCaa                                                                       <  2:1201798/140‑1 (MQ=255)
                                                                                      tACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACAt                                                       <  1:587490/140‑1 (MQ=35)
                                                                                          ggTGATGCTGCCAACTTACTGTTTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTCTCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgc                                                   <  1:1416469/140‑1 (MQ=17)
                                                                                           gTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcg                                                  >  1:1511910/1‑140 (MQ=32)
                                                                                           gTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcg                                                  >  1:980166/1‑140 (MQ=32)
                                                                                           gTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcg                                                  <  2:128316/140‑1 (MQ=32)
                                                                                           gTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcg                                                  <  2:77227/140‑1 (MQ=32)
                                                                                           gTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcg                                                  >  2:874790/1‑140 (MQ=32)
                                                                                              aTGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTa                                               >  1:1781540/1‑140 (MQ=32)
                                                                                              aTGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTa                                               <  1:417440/140‑1 (MQ=32)
                                                                                              aTGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTa                                               >  2:1875751/1‑140 (MQ=32)
                                                                                              aTGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTa                                               <  1:1069320/140‑1 (MQ=32)
                                                                                                gctgcCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTAtc                                             >  1:199550/1‑140 (MQ=32)
                                                                                                gctgcCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTAtc                                             >  1:199559/1‑140 (MQ=32)
                                                                                                         ttACTGATTTAGTGTATGATGGTGTTTTTGATTTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACAGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGAAATCAGAGCTATCTCTGCactc                                    >  2:1189970/1‑140 (MQ=11)
                                                                                                          tACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATAAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCa                                   >  2:1350838/1‑140 (MQ=17)
                                                                                                           aCTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCAc                                  <  1:1689531/140‑1 (MQ=32)
                                                                                                           aCTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCAc                                  >  2:1026662/1‑140 (MQ=32)
                                                                                                           aCTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCAc                                  <  1:287440/140‑1 (MQ=32)
                                                                                                              gATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGc                               >  1:1137111/1‑140 (MQ=32)
                                                                                                              gATTTAGTGTATGATGGTGTTTATGAGGTTCTCCAGTGGCTTCTGTTTCTATAAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGc                               >  1:946454/1‑140 (MQ=17)
                                                                                                                tttAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCg                             <  2:1088768/140‑1 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:346453/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  1:1468166/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:424208/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:346966/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:346602/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:1698583/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:1701142/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:1135841/1‑140 (MQ=32)
                                                                                                                   aGTGTATGATGGTGTATTTGAGGTTCTCCAGTGGCTTCGGTTTCTATCAGCTGTCCCTCCTGATCAGCTACAGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:452538/1‑140 (MQ=255)
                                                                                                                     tgtATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaaaa                        <  2:1028039/140‑1 (MQ=32)
                                                                                                                     tgtATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaaaa                        >  1:1276039/1‑140 (MQ=32)
                                                                                                                      gtttgatgGTGTTTTTGTGGTTCTCGTGTGGTTTATGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGTCGGGGTGGTGAGTAAATGTAAATTAACTGGCGGAATTGAGCGCTATCTCTGCTCTCACTGCCGTatata                       <  2:1818252/137‑5 (MQ=2)
                                                                                                                         tgatgGTGTTTTTGAGGTTCTCCAGTGGCTTCTTTTTCTATCAGCTTTACCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATg                    >  2:1263223/1‑140 (MQ=12)
                                                                                                                         tgatgGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATg                    >  1:1889527/1‑140 (MQ=32)
                                                                                                                         tgatgGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATAAGCTGTCCCTCCTGTTCAGCTACAGACGGGGAGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCAATCTCAGCACTCACTGCCGTAAAACAtt                    >  1:1373407/1‑139 (MQ=11)
                                                                                                                           atgGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGc                  <  1:935581/140‑1 (MQ=32)
                                                                                                                             ggTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCaa                <  1:887540/140‑1 (MQ=32)
                                                                                                                             ggTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCAATCTCTGCTCTCACTGCCGTAAAACATGGCaa                >  2:242252/1‑140 (MQ=17)
                                                                                                                               tgtTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACt              >  1:762990/1‑140 (MQ=32)
                                                                                                                               tgtTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCACCAGTTCAGCTACTTACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCAATCTCTGCTCTCACTGCCGTAAAACATGGCAACt              >  1:426915/1‑140 (MQ=255)
                                                                                                                                 tttttGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGc            >  1:1600750/1‑140 (MQ=32)
                                                                                                                                 tttttGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGc            >  2:306057/1‑140 (MQ=32)
                                                                                                                                   tttGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAg          <  1:1234415/140‑1 (MQ=32)
                                                                                                                                   tttGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAg          <  1:717233/140‑1 (MQ=32)
                                                                                                                                         gTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACt    >  2:1076650/1‑140 (MQ=32)
                                                                                                                                           tctcCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTa  >  2:351242/1‑140 (MQ=11)
                                                                                                                                           |                                                                                                                                           
CGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTA  >  NC_000913/1049688‑1049966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: