Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,086,485 G→T 6.2% P12Q (CCA→CAA)  pgaD ← biofilm PGA synthase PgaCD, regulatory subunit; c‑di‑GMP‑stimulated activity and dimerization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,086,4850GT 6.2% 228.1 / 2.7 64P12Q (CCA→CAA) pgaDbiofilm PGA synthase PgaCD, regulatory subunit; c‑di‑GMP‑stimulated activity and dimerization
Reads supporting (aligned to +/- strand):  ref base G (35/25);  new base T (2/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAG  >  NC_000913/1086363‑1086625
                                                                                                                          |                                                                                                                                            
ggCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGta                                                                                                                             >  1:1128823/1‑140 (MQ=255)
 gCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGtaa                                                                                                                            <  2:909505/140‑1 (MQ=255)
  cTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGtaat                                                                                                                           >  1:661267/1‑140 (MQ=255)
     cTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTaataat                                                                                                                        >  2:1330268/1‑140 (MQ=255)
             gCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGt                                                                                                                <  1:1844044/140‑1 (MQ=255)
                      gTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTaa                                                                                                       <  2:1354711/140‑1 (MQ=255)
                        aataaCCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTTATAATTAAATTGTTCATATTAAcc                                                                                                     <  1:286935/140‑1 (MQ=255)
                         ataaCCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACct                                                                                                    >  1:818915/1‑140 (MQ=255)
                                 tCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAAt                                                                                            >  1:1774833/1‑140 (MQ=255)
                                     cagATCCCTGGCGAATAAGAATCTGAACACCGCAAATAATGTCCACAAGTTGGTTGAGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGACGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCcg                                                                                        <  2:386403/140‑1 (MQ=255)
                                        aTCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTTGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGa                                                                                     >  2:169920/1‑140 (MQ=255)
                                        aTCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTTGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGa                                                                                     >  2:170247/1‑140 (MQ=255)
                                         tCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGAt                                                                                    <  1:399250/140‑1 (MQ=255)
                                         tCCATGGCGAATAAGAATATGAACAAAGCAAATATTGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATCTTAACCTCTCAGAATCCCGCGAt                                                                                    <  1:398351/140‑1 (MQ=255)
                                          ccATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATc                                                                                   <  2:1922425/140‑1 (MQ=255)
                                          ccATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATc                                                                                   <  2:1404195/140‑1 (MQ=255)
                                                  aaTAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTa                                                                           >  1:1560109/1‑140 (MQ=255)
                                                  aaTAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTa                                                                           >  1:585127/1‑140 (MQ=255)
                                                  aaTAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTa                                                                           >  1:29802/1‑140 (MQ=255)
                                                   aTAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTAc                                                                          >  1:508946/1‑140 (MQ=255)
                                                            tGAACAACGCAAATAATGTCCACAAGATGGTTTTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGTGTCGTAATAATTAAATTTTTCATATTAACATCTCAGAATCCCGCGATCGGGACTTCCCCAACGGgc                                                                 >  2:829462/1‑140 (MQ=255)
                                                            tGAACAACGCAAATAATGTCCACAAGAAGGTTGTGGCAACATAATCAACCATTAAACGTACTGGTTATTTTCGGGTAGTAATAATTAAATTGTTCATATTAACCGCTCAGAAACCCGCGATCGGGACTTACCCAAAGGgc                                                                 >  2:829157/1‑140 (MQ=255)
                                                               acaacGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGcgcg                                                              >  1:419078/1‑140 (MQ=255)
                                                               acaacGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGcgcg                                                              >  1:630652/1‑140 (MQ=255)
                                                               acaacGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGcgcg                                                              <  1:694867/140‑1 (MQ=255)
                                                               acaacGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGcgcg                                                              >  1:774859/1‑140 (MQ=255)
                                                                caacGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTTGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGt                                                             <  1:170247/140‑1 (MQ=255)
                                                                caacGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTTGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGt                                                             <  1:169920/140‑1 (MQ=255)
                                                                 aacGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGtt                                                            <  1:911873/140‑1 (MQ=255)
                                                                  acGCAAATAATGTCCACACGATGGTGGGGGCAGCATACTCAACCAGGAAGCGTCCGGGTGGTTGGCGGGTCGTGAGAGGTCAGTTGGTCAGCTTCAGCGCGCAGATTCCCGGGAGCGGGACTTACCCATGGGGCGCGGTg                                                           >  2:1493414/1‑140 (MQ=255)
                                                                  acGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTATCCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTg                                                           >  1:1087758/1‑140 (MQ=255)
                                                                  acGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTg                                                           >  1:935279/1‑140 (MQ=255)
                                                                  acGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTg                                                           >  2:1662261/1‑140 (MQ=255)
                                                                  acGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTg                                                           >  1:696713/1‑140 (MQ=255)
                                                                  acGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTg                                                           >  1:1559037/1‑140 (MQ=255)
                                                                  acGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTg                                                           >  1:1300829/1‑140 (MQ=255)
                                                                   ctcAAATAATCTCCAAAATATGTTTTAGTCAACAAAAAAAAACAGAAAACTTAATGTTGAAAGTCGGGTCGTAATAATTAAATATTTCATATTAACCTCTCAGAATCCCTCGATCGGTACTTACCCAACTGGCGCGTTga                                                          >  2:1923624/3‑139 (MQ=255)
                                                                   cGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGc                                                          >  1:1697406/1‑140 (MQ=255)
                                                                       aataatGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCtttt                                                      <  2:189878/140‑1 (MQ=255)
                                                                       aataatGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCtttt                                                      <  2:189697/140‑1 (MQ=255)
                                                                       aataatGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCtttt                                                      <  2:73309/140‑1 (MQ=255)
                                                                         tattGTCCACAAGATTGTTGTGGCATCATAATCATCCAGTAAACGTACTGGTGATTGTCGGGTCGTATTATTTAAATTGTTCATATTAACCTCTCAGACTCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTa                                                    <  1:680702/137‑1 (MQ=255)
                                                                         taatGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTa                                                    <  1:1294266/140‑1 (MQ=255)
                                                                           atGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAgg                                                  >  2:189430/1‑140 (MQ=255)
                                                                            tGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGc                                                 <  1:1860647/140‑1 (MQ=255)
                                                                            tGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGc                                                 <  2:243837/140‑1 (MQ=255)
                                                                             gTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCa                                                <  1:1698405/140‑1 (MQ=255)
                                                                              tCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCAt                                               >  1:1054383/1‑140 (MQ=255)
                                                                              tCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCAt                                               >  1:875751/1‑140 (MQ=255)
                                                                              tCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGACGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGTGCGCGTTGCTTTTTAGGCAt                                               >  1:1054342/1‑140 (MQ=255)
                                                                                      aTGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACATCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGa                                       >  1:1097115/1‑140 (MQ=255)
                                                                                        ggTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACt                                     >  1:1363197/1‑140 (MQ=255)
                                                                                          ttGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCg                                   >  2:1651374/1‑140 (MQ=255)
                                                                                            gtgGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCgtg                                 <  2:1640127/140‑1 (MQ=255)
                                                                                            gtgGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCgtg                                 <  2:704650/140‑1 (MQ=255)
                                                                                               gCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTaa                              >  1:1859057/1‑140 (MQ=255)
                                                                                                  aCATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATATTTAACTTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATg                           <  1:75590/140‑1 (MQ=255)
                                                                                                   cATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGa                          >  2:272948/1‑140 (MQ=255)
                                                                                                    aTAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATTTTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGAATCGTGTAAATGAt                         >  1:687399/1‑140 (MQ=255)
                                                                                                                tAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTATGCATCAACATGACTCGTGTAAATGATACCAATGTCGtt             >  2:258270/1‑140 (MQ=255)
                                                                                                                tAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGtt             >  2:776753/1‑140 (MQ=255)
                                                                                                                   acttACTGGTGTTTGTCGGTTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGcc          <  2:1066241/137‑1 (MQ=255)
                                                                                                                   aCGTCCTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGcc          <  2:1599186/140‑1 (MQ=255)
                                                                                                                   aCGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGcc          <  2:587980/140‑1 (MQ=255)
                                                                                                                          ggTTATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAg  >  2:796479/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGCCTTACCCACCGGCCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:493509/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:525493/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:527190/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:501143/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:795979/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:796450/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:1373053/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  1:151387/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:909779/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAAAGTCGTTGCCAGGCTCa   >  2:512697/1‑140 (MQ=255)
                                                                                                                          ggTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTGTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCa   >  2:402342/1‑140 (MQ=255)
                                                                                                                          |                                                                                                                                            
GGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGCTCAG  >  NC_000913/1086363‑1086625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: