Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,092,203 C→T 5.1% A29A (GCG→GCA pgaA ← biofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,092,2030CT 5.1% 271.7 / 3.8 78A29A (GCG→GCApgaAbiofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein
Reads supporting (aligned to +/- strand):  ref base C (42/32);  new base T (2/2);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

AATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTA  >  NC_000913/1092068‑1092342
                                                                                                                                       |                                                                                                                                           
aaTCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCgg                                                                                                                                         >  1:1884692/1‑140 (MQ=255)
  tCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGa                                                                                                                                       >  1:1064626/1‑140 (MQ=255)
  tCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGa                                                                                                                                       >  1:1827905/1‑140 (MQ=255)
  tCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGa                                                                                                                                       >  1:263450/1‑140 (MQ=255)
  tCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGa                                                                                                                                       >  1:1680929/1‑140 (MQ=255)
  tCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGa                                                                                                                                       >  1:620827/1‑140 (MQ=255)
             tCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCTGCGGGACTCGCTGCTaa                                                                                                                            >  1:794348/1‑140 (MQ=255)
             tCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCTGCGGGACTCGCTGCTaa                                                                                                                            >  1:794294/1‑140 (MQ=255)
             tCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTaa                                                                                                                            >  2:1761390/1‑140 (MQ=255)
                      tGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGc                                                                                                                   >  1:206958/1‑140 (MQ=255)
                           aTTTCTTAGTGCTGATTTTAGTGCAAACAATTACAAATCTTGCTGAGTATTACCCTATCGAGCTTCAATAATCAAAGCATCAAATGCTTTATTAACATCAATCTTCTCCGCGGGACTCGCTGCTAAAAATTCTTCAGTaa                                                                                                              >  2:953812/1‑140 (MQ=255)
                           aTTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTaa                                                                                                              >  2:1598485/1‑140 (MQ=255)
                              gCTGAGTGCTGATTTTAGTGCAAACCATGTCAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTaagaa                                                                                                           >  2:1839337/1‑140 (MQ=255)
                              gCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTaagaa                                                                                                           >  2:256717/1‑140 (MQ=255)
                                  aGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACTCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGttt                                                                                                       >  1:188910/1‑140 (MQ=255)
                                    tGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCa                                                                                                     <  1:1186282/140‑1 (MQ=255)
                                              aGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCAttt                                                                                           >  1:1002784/1‑140 (MQ=255)
                                              aGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCAttt                                                                                           >  2:1650954/1‑140 (MQ=255)
                                              aGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCAttt                                                                                           >  2:403918/1‑140 (MQ=255)
                                              aGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCAttt                                                                                           >  1:1002764/1‑140 (MQ=255)
                                                   aaaCCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGttt                                                                                      >  2:708078/1‑140 (MQ=255)
                                                     aCCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTc                                                                                    >  2:1089211/1‑140 (MQ=255)
                                                          gACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGcc                                                                               >  1:616075/1‑139 (MQ=255)
                                                           aCAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCAc                                                                              <  2:707588/140‑1 (MQ=255)
                                                               aGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCttt                                                                          >  1:1472425/1‑140 (MQ=255)
                                                               aGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAAAGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCttt                                                                          >  2:90112/1‑140 (MQ=255)
                                                               aGCTGGCTGAGTATTACCCTTGCGAGCGTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCgtt                                                                          >  2:1216295/1‑140 (MQ=255)
                                                                 ctggctgAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCttttt                                                                        >  1:66946/1‑140 (MQ=255)
                                                                  tggctgAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTc                                                                       <  1:1211891/140‑1 (MQ=255)
                                                                  tggctgAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTc                                                                       <  1:1426217/140‑1 (MQ=255)
                                                                     ctgAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGc                                                                    <  2:555255/140‑1 (MQ=255)
                                                                     ctgAGTATTACCCTTGCGAGATTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGc                                                                    <  2:812289/140‑1 (MQ=255)
                                                                        aGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTAc                                                                 <  1:453136/140‑1 (MQ=255)
                                                                        aGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTAc                                                                 <  1:453150/140‑1 (MQ=255)
                                                                         gTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACt                                                                <  1:1743596/140‑1 (MQ=255)
                                                                         gTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACt                                                                <  2:1014523/140‑1 (MQ=255)
                                                                             tACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGaa                                                            <  1:1315978/140‑1 (MQ=255)
                                                                             tACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGaa                                                            <  2:1673579/140‑1 (MQ=255)
                                                                              aCCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAAt                                                           <  2:1050684/140‑1 (MQ=255)
                                                                                  ttGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACAt                                                       >  1:1062989/1‑140 (MQ=255)
                                                                                   tgctatcTTCAATAATAAATGCATCATAGTCTTTATTCTCATCACTCGTCGCCGCGGGACTCGCTGCTACAAATGCTTCATTATAAAGTTTCACATCCCATTTGGATTTAGGGCTCCTTTTTCTGCTACTTGAATACATc                                                      <  2:800401/134‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGTATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:55157/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:1428375/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:280400/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:280284/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  1:6705/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:188910/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:1751216/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:1712087/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:1684648/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:878533/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:1427566/140‑1 (MQ=255)
                                                                                   tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  2:1518377/140‑1 (MQ=255)
                                                                                   tGCGAGCGTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATc                                                      <  1:424540/140‑1 (MQ=255)
                                                                                    gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATcc                                                     >  1:627036/1‑140 (MQ=255)
                                                                                    gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCATCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAATCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATcc                                                     >  2:1808355/1‑140 (MQ=255)
                                                                                        gCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTa                                                 <  1:637979/140‑1 (MQ=255)
                                                                                           tCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCTGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTa                                              >  2:609754/1‑140 (MQ=255)
                                                                                                    aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTa                                     >  2:1737005/1‑140 (MQ=255)
                                                                                                    aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTa                                     >  2:683439/1‑140 (MQ=255)
                                                                                                    aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTa                                     >  2:683371/1‑140 (MQ=255)
                                                                                                    aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTa                                     >  1:1435579/1‑140 (MQ=255)
                                                                                                    aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTa                                     >  1:1268630/1‑140 (MQ=255)
                                                                                                      tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTAtt                                   >  2:817115/1‑140 (MQ=255)
                                                                                                      tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGATGCTAAAAATTCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTAtt                                   >  2:278489/1‑140 (MQ=255)
                                                                                                          tcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCa                               <  2:206958/140‑1 (MQ=255)
                                                                                                           catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCaa                              >  2:1066394/1‑140 (MQ=255)
                                                                                                           catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCaa                              >  1:881137/1‑140 (MQ=255)
                                                                                                              aGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAt                           >  2:610010/1‑140 (MQ=255)
                                                                                                                gCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAtct                         >  1:1593533/1‑140 (MQ=255)
                                                                                                                     attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAAtctctctc                    >  1:1443620/1‑140 (MQ=255)
                                                                                                                     attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCAGGTATTGCCAAAAtctctctc                    >  1:1901270/1‑140 (MQ=255)
                                                                                                                       taACAGCACTCTTCGCTGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTg                  <  2:794348/140‑1 (MQ=255)
                                                                                                                       taACAGCACTCTTCGCTGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTg                  <  2:794294/140‑1 (MQ=255)
                                                                                                                        aaCAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGt                 >  1:688453/1‑140 (MQ=255)
                                                                                                                          cAGCACTCTTCGCCGCGGGACTCGCTGCTATAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGTGCAACTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt               <  2:316059/140‑1 (MQ=255)
                                                                                                                          cAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt               <  2:1192274/140‑1 (MQ=255)
                                                                                                                          cAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACATTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt               <  1:1731204/140‑1 (MQ=255)
                                                                                                                                     gccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAgg    <  2:263450/140‑1 (MQ=255)
                                                                                                                                       cgcgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTa  >  2:837289/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
AATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTA  >  NC_000913/1092068‑1092342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: