Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,113,077 C→A 5.6% R739S (CGT→AGT)  opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,113,0770CA 5.6% 389.8 / 7.0 108R739S (CGT→AGT) opgHOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
Reads supporting (aligned to +/- strand):  ref base C (59/42);  new base A (3/3);  total (63/45)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 108 total aligned reads displayed.

TCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACGTTGAA  >  NC_000913/1112942‑1113211
                                                                                                                                       |                                                                                                                                      
tCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAATCGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCa                                                                                                                                    >  1:258531/1‑140 (MQ=255)
tCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCa                                                                                                                                    >  1:1466205/1‑140 (MQ=255)
tCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCa                                                                                                                                    >  1:1447984/1‑140 (MQ=255)
tCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTAGTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAAGCGTcg                                                                                                                                    >  1:1887681/1‑139 (MQ=255)
tCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGAACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATAGtaa                                                                                                                                    >  1:8860/1‑138 (MQ=255)
tCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGAACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATAGtaa                                                                                                                                    >  1:565070/1‑138 (MQ=255)
tCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGAACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATAGtaa                                                                                                                                    >  1:5649/1‑138 (MQ=255)
   ccGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACg                                                                                                                                 <  1:877405/140‑1 (MQ=255)
    tgtttgtttCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACAAAACGCTGGAAACTGTTCCTGTTCCCGGTAGAGTTTTCGCCGCAGCTGGTTCTTTTTGATACAGATCGGTTCCTTGAGTTGAATAGtgttgta                                                                                                                                <  1:788240/139‑7 (MQ=255)
    cGTTTGTTTCGGTGTTTTCCTGCAGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCACGGAAGTGTATTCGCCGCCTCAGGTTATTTTTGATAACGTTCGGGTCCTTGAGTTGAATAGtgttgta                                                                                                                                <  1:787854/140‑7 (MQ=255)
      tttgtttCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTc                                                                                                                              >  1:1152010/1‑140 (MQ=255)
      tttgtttCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTc                                                                                                                              >  1:1240544/1‑140 (MQ=255)
        tgtttCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTccc                                                                                                                            >  2:397965/1‑140 (MQ=255)
        tgtttCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTccc                                                                                                                            <  2:252206/140‑1 (MQ=255)
              ggTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTtgatg                                                                                                                      >  2:622864/1‑140 (MQ=255)
               gTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTtgatga                                                                                                                     <  2:1776631/140‑1 (MQ=255)
                     tCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCtt                                                                                                               <  2:226098/140‑1 (MQ=255)
                     tCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCtt                                                                                                               <  2:226080/140‑1 (MQ=255)
                        aGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATTCCGTTCTTTTCATTTATATTAATCGTCAACGCTCCATTTATTTTGGGttttt                                                                                                            <  1:1463254/140‑3 (MQ=255)
                          ccGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGATGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGTATCGTCAACGCTCCCTTGATGATGGCTTTATGc                                                                                                          <  1:573138/140‑1 (MQ=255)
                            gTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCAc                                                                                                        >  1:1273564/1‑140 (MQ=255)
                            gTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCAc                                                                                                        >  1:1273947/1‑140 (MQ=255)
                             tGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACg                                                                                                       >  2:948552/1‑140 (MQ=255)
                             tGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACg                                                                                                       >  2:947837/1‑140 (MQ=255)
                             tGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACg                                                                                                       <  2:1346113/140‑1 (MQ=255)
                             tGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACg                                                                                                       <  1:470904/140‑1 (MQ=255)
                             tGCCACCGGTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAAGCGTCAACGCTCCCTTGATGATGGCTTTATGCACg                                                                                                       <  2:88967/140‑1 (MQ=255)
                              gCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGc                                                                                                      <  1:221354/140‑1 (MQ=255)
                              gCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGc                                                                                                      <  2:1681401/140‑1 (MQ=255)
                              gCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGc                                                                                                      <  1:1383563/140‑1 (MQ=255)
                              gCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGc                                                                                                      <  2:1387041/140‑1 (MQ=255)
                              gCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCGTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGc                                                                                                      <  1:221394/140‑1 (MQ=255)
                               ccACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCa                                                                                                     <  2:1193624/140‑1 (MQ=255)
                               ccACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCa                                                                                                     <  1:785501/140‑1 (MQ=255)
                               ccACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCa                                                                                                     <  1:785497/140‑1 (MQ=255)
                               ccACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCa                                                                                                     <  1:1137460/140‑1 (MQ=255)
                               ccACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCa                                                                                                     <  1:397965/140‑1 (MQ=255)
                                    gTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGtt                                                                                                >  2:136454/1‑140 (MQ=255)
                                    gTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAAACGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGtt                                                                                                >  2:136660/1‑140 (MQ=255)
                                       ggTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTaa                                                                                             >  1:334299/1‑140 (MQ=255)
                                             cgcACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTc                                                                                       >  1:184552/1‑140 (MQ=255)
                                             cgcACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTc                                                                                       >  2:312318/1‑140 (MQ=255)
                                             cgcACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTc                                                                                       >  1:463451/1‑140 (MQ=255)
                                             cgcACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTc                                                                                       >  1:1418986/1‑140 (MQ=255)
                                                  cAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTa                                                                                  >  1:1463659/1‑140 (MQ=255)
                                                  cAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTa                                                                                  >  1:1463692/1‑140 (MQ=255)
                                                   aaaCGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTaa                                                                                 <  1:873267/140‑1 (MQ=255)
                                                          ggAAACTGTTCCTGATCCCGGAAGAGTATTAGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTg                                                                          >  1:684893/1‑140 (MQ=255)
                                                            aaaCTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGc                                                                        <  1:601626/140‑1 (MQ=255)
                                                            aaaCTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGc                                                                        <  1:601777/140‑1 (MQ=255)
                                                            aaaCTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGc                                                                        <  1:279396/140‑1 (MQ=255)
                                                            aaaCTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGAGTTTAACCCGTCATTTAACGCTCTGGc                                                                        <  2:517171/140‑1 (MQ=255)
                                                            aaaCTGTTCCGGATCCCGGCAGAGTATGCGCCGCCGCTGGTGCTGGTTGATCCCGCTCGGGTCCTTGAGATGCATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGc                                                                        <  1:930608/140‑1 (MQ=255)
                                                              aCTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCaa                                                                      >  2:1081442/1‑140 (MQ=255)
                                                                  ttCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGc                                                                  >  1:172900/1‑140 (MQ=255)
                                                                   tCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCa                                                                 >  1:1469410/1‑140 (MQ=255)
                                                                          cccGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGa                                                          >  1:466742/1‑140 (MQ=255)
                                                                                    tataCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTAGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACTCTCTGGCAACCGCAATGGCGACCGCGCGTCa                                                >  1:819027/1‑140 (MQ=255)
                                                                                    tATTCGCCGCCGCAGGTGCTGGTTGCTACCGATCGGTTCCGTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGGTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCa                                                >  1:1328629/1‑140 (MQ=255)
                                                                                    tATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCa                                                >  1:1404253/1‑140 (MQ=255)
                                                                                    tATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCa                                                >  1:1407968/1‑140 (MQ=255)
                                                                                    tATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCa                                                >  1:819195/1‑140 (MQ=255)
                                                                                      ttCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCAcc                                              >  1:130945/1‑140 (MQ=255)
                                                                                       tCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACcg                                             >  1:952909/1‑140 (MQ=255)
                                                                                       tCGCCGCCGCAGGTGCTGGTTGATACCGATCGGGTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACcg                                             <  1:1275008/140‑1 (MQ=255)
                                                                                       tCGCCGCCGCAGGTGCTGGATGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCATTATGCACGCAGTGTTTAACCCGTCATTTAACGCACTGGCAACCGCAATGGCGACCGCGCGTCACcg                                             <  2:1157666/140‑1 (MQ=255)
                                                                                          ccgccgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCgcgc                                          <  1:923334/140‑1 (MQ=255)
                                                                                          ccgccgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCgcgc                                          <  1:798466/140‑1 (MQ=255)
                                                                                             ccgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAg                                       >  1:1668322/1‑140 (MQ=255)
                                                                                             ccgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAg                                       >  1:1668586/1‑140 (MQ=255)
                                                                                              cgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGt                                      >  2:461530/1‑140 (MQ=255)
                                                                                              cgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGt                                      >  2:1407114/1‑140 (MQ=255)
                                                                                              cgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGt                                      >  2:864154/1‑140 (MQ=255)
                                                                                              cgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGt                                      >  2:750116/1‑140 (MQ=255)
                                                                                              cgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGt                                      >  2:704008/1‑140 (MQ=255)
                                                                                              cgcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGt                                      >  2:1274713/1‑140 (MQ=255)
                                                                                               gcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTa                                     <  2:1807846/140‑1 (MQ=255)
                                                                                               gcAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTa                                     <  2:1807845/140‑1 (MQ=255)
                                                                                                          ttGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGaa                          >  1:1256960/1‑140 (MQ=255)
                                                                                                             aTACCGATCGGTTCCTTGAGTTGAATCGTCAACGCTCCCTTGATGATGGCTTTAGGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATc                       <  2:1152619/140‑1 (MQ=255)
                                                                                                             aTACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATc                       >  2:763620/1‑140 (MQ=255)
                                                                                                             aTACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATc                       >  2:341314/1‑140 (MQ=255)
                                                                                                              tACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGAGCTGGAAATCg                      >  2:58441/1‑140 (MQ=255)
                                                                                                                ccGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGcc                    >  1:517811/1‑140 (MQ=255)
                                                                                                                   aTCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCTGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGt                 >  1:518958/1‑140 (MQ=255)
                                                                                                                   aTCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGt                 >  1:1738223/1‑140 (MQ=255)
                                                                                                                     cGGTTCCTTGAGCTGAAGCGTCAACGCTCCCTTGATGATGGCTTTCTGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAACTCGCCCGTGa               <  1:88740/140‑1 (MQ=255)
                                                                                                                        ttCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCg            >  1:204378/1‑140 (MQ=255)
                                                                                                                        ttCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCg            >  1:204546/1‑140 (MQ=255)
                                                                                                                        ttCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCg            <  1:1438066/140‑1 (MQ=255)
                                                                                                                        ttCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCg            >  1:925271/1‑140 (MQ=255)
                                                                                                                        ttCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCg            <  2:1292157/140‑1 (MQ=255)
                                                                                                                        ttCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCg            >  2:1300125/1‑140 (MQ=255)
                                                                                                                        ttCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCg            <  1:1589826/140‑1 (MQ=255)
                                                                                                                             tGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACg       <  2:1240544/140‑1 (MQ=255)
                                                                                                                              gagaTGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACGt      <  1:58441/140‑1 (MQ=255)
                                                                                                                                 aTGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGGGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGACACCGCGCCAGTCAGGTGCAGGACATCGCCCGGGACCGCCACGTTGa   >  2:1850368/1‑140 (MQ=255)
                                                                                                                                  tGAATGGTCATCGCTCGCTTGATGATGGCTTTATGCTCGCTGTGTTTAACCCGTCATTTATCGCTCTGGCAACCGCATTGGCGACCGCGCGTCACCGCGCGAGTAAGGTGGTGGAAATCGCCCGTGAGCGCCACGTTGaa  >  2:1372613/1‑140 (MQ=255)
                                                                                                                                  tGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACGTTGaa  >  2:1183817/1‑140 (MQ=255)
                                                                                                                                  tGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACGTTGaa  >  2:311573/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TCACCGTTTGTTTCGGTGATTTCCAGCCGTGCCACCGTTGGTCTGCGCACCAAACGCTGGAAACTGTTCCTGATCCCGGAAGAGTATTCGCCGCCGCAGGTGCTGGTTGATACCGATCGGTTCCTTGAGATGAATCGTCAACGCTCCCTTGATGATGGCTTTATGCACGCAGTGTTTAACCCGTCATTTAACGCTCTGGCAACCGCAATGGCGACCGCGCGTCACCGCGCCAGTAAGGTGCTGGAAATCGCCCGTGACCGCCACGTTGAA  >  NC_000913/1112942‑1113211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: