Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,386,010 C→A 6.1% S167* (TCG→TAG)  ycjF → UPF0283 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,386,0100CA 6.1% 259.0 / 2.1 74S167* (TCG→TAG) ycjFUPF0283 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (35/34);  new base A (3/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGAGTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGA  >  NC_000913/1385871‑1386148
                                                                                                                                           |                                                                                                                                          
aCAGAGTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGTAGCGCGTGTTTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAATCTGGCGCAGCAGGCGGGTATTGATCAGTc                                                                                                                                            >  1:1562478/1‑140 (MQ=255)
     gTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATc                                                                                                                                       >  1:509100/1‑140 (MQ=255)
     gTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATc                                                                                                                                       >  1:662310/1‑140 (MQ=255)
     gTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATc                                                                                                                                       >  2:1888533/1‑140 (MQ=255)
     gTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATc                                                                                                                                       >  2:1369681/1‑140 (MQ=255)
     gTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTAGCATc                                                                                                                                       >  1:1666581/1‑140 (MQ=255)
     gTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTAGCATc                                                                                                                                       >  1:1666567/1‑140 (MQ=255)
     gTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTAGCATc                                                                                                                                       >  1:88237/1‑140 (MQ=255)
            cgcTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCt                                                                                                                                >  1:1489499/1‑140 (MQ=255)
                 aTGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAAc                                                                                                                           <  1:475906/140‑1 (MQ=255)
                  tGGCGCTTGCGACAGCGCGCCCATGAACGCGACGTAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACg                                                                                                                          <  1:40881/140‑1 (MQ=255)
                  tGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACg                                                                                                                          <  1:41020/140‑1 (MQ=255)
                  tGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACg                                                                                                                          <  1:1373297/140‑1 (MQ=255)
                  tGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACg                                                                                                                          <  1:1567847/140‑1 (MQ=255)
                   cgcgcTTTCTACATCGCGCGGCTTATCGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGc                                                                                                                         <  1:282249/139‑1 (MQ=255)
                       cTTGCGTCAGCGCGCCCTTGAACGCGTCGTAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAATAACTGGCGCAGCATGCGGGTTTTGTTAATTATCATCCGGCGCTGCTACGCTgtt                                                                                                                     <  1:719726/140‑3 (MQ=255)
                       cTTGCGACAGCGCGCCCATGAACGCGACGATGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGTAAAACTGGCGCTGCAGGAGGTTATTGTTCATTATAATCCGGCGCTGCTACGCTgtt                                                                                                                     <  1:718325/140‑3 (MQ=255)
                       cTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGt                                                                                                                     <  1:1556728/140‑1 (MQ=255)
                       cTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGt                                                                                                                     <  2:223241/140‑1 (MQ=255)
                       cTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGt                                                                                                                     <  2:605117/140‑1 (MQ=255)
                        ttGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGTTCAGTCGCATCCGGCGCTGCAACGCTGGTa                                                                                                                    <  2:452206/140‑1 (MQ=255)
                        ttGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTa                                                                                                                    <  1:700967/140‑1 (MQ=255)
                         tGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTAt                                                                                                                   >  2:1565111/1‑140 (MQ=255)
                            gACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGcc                                                                                                                <  2:727316/140‑1 (MQ=255)
                                 cgcgcCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAAt                                                                                                           >  1:1343118/1‑140 (MQ=255)
                                       cATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGa                                                                                                     <  1:784224/140‑1 (MQ=255)
                                              gcgACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCaa                                                                                              >  2:1287798/1‑140 (MQ=255)
                                                   gaAGCGCGTGACTTTAGGCATTGCCCCGGCCCGGGCAAAGGCCGCGCTTTTTGCGAACACCGGCCGCAGCCGGCGGGTATTGATCAGACGCACCCGGGGCTGAAACGCTGGTATGCCTCAAACCATGAAACGCAAAACGa                                                                                         <  2:948672/140‑1 (MQ=255)
                                                        gcgTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTg                                                                                    <  2:431463/140‑1 (MQ=255)
                                                          gTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGTCCGTGaa                                                                                  >  1:1823584/1‑140 (MQ=255)
                                                            gATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACTACCGTGAAGt                                                                                >  1:1115778/1‑140 (MQ=255)
                                                               ttATTGCATCGCCACGGCCCGGGCAATGGCCGCGCATTTTGCGAAAACCTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAACACGACCGTGAAGTGGt                                                                             <  1:1689145/140‑1 (MQ=255)
                                                               ttATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCTGGCGGTTATTGATCAGTCGCATCCGGCGCTGAAACGCTGGTATGCCTCAATCAATTAAACGCAAAACGACCGTGAAGTGGt                                                                             <  2:1325628/140‑1 (MQ=255)
                                                                 tttGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTTTTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCa                                                                           <  2:527267/139‑1 (MQ=255)
                                                                   tGCATTGCCACGGCACGGGCAAAGGCCGCGCATTTTTCGAACAACTGGCGCAGCTGGCGGGTATTGATCAGTCGCATCCGGGGCTGCAACGCTGGTATGCATCAATCCATGAAACGCAAAACGACCGTGAAGTGGACAGc                                                                         <  2:1051575/140‑1 (MQ=255)
                                                                   tGCATCGCCACGGCACGGGAAACGGCCGCGCTTTTTGCTAAAAACTGGCGCAGCTGGGGGTTATTGAACAGTCGCATCCGGCGCTGCACCGCTGGTAGGCCTCAATCCATGAAACGAAACACGACCGTGAAGTGGTCAGc                                                                         <  1:302383/140‑1 (MQ=255)
                                                                   tGCATCCCCCCGGCACGGGCAAAGGCCGCGAATTTTGCTAAAAACTGGCGCAGCAGGCGGGTTTTGACCATTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAAACCATGAAACGCAACACGACCGTGAAGTGGTCAGc                                                                         <  1:302010/140‑1 (MQ=255)
                                                                   tGCATAGCCACGGCAGGGGAAATGGCCGCGAATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGTTATGCCTAAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGc                                                                         <  1:569771/140‑1 (MQ=255)
                                                                          ccAGGGCACGGGAAAAGGCCGCGATTTTTGCTAAAAACTGGCGCAGCTGGGGGTTATTTATCCGTCGCATCCGGCGCTGAAACGCTGTTAGGCCTCAATCCATTAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATg                                                                  <  1:331921/140‑1 (MQ=255)
                                                                          ccACGGCAGGGGCAACGGCCGCGCTTTTTGCGAAAAACGGGCGCCGCAGGGGGGTTTTGATCCGTCGCATCCGGCGCTGCACCGGGGGTATGCCTCAATCCATGAACCGCAAAACGACCGTGAAGTGGTCAGCCTGTATg                                                                  <  1:1422140/140‑1 (MQ=255)
                                                                          ccACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATg                                                                  >  1:1336105/1‑140 (MQ=255)
                                                                          ccACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATg                                                                  >  1:1336630/1‑140 (MQ=255)
                                                                          ccACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATg                                                                  >  1:1750227/1‑140 (MQ=255)
                                                                          ccACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATg                                                                  >  1:1336728/1‑140 (MQ=255)
                                                                          ccACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATg                                                                  >  1:1750073/1‑140 (MQ=255)
                                                                           cccggcacggGCAACGGCCGCGAATTTTGCGAAAACCGGGCGCAGCAGGCGGGTTTTTATCAGTCGCATCCGGCGCTGAAACGCTGTTAGGCCAAAATCCTTCAACCCCAAAACGACCGTGAAGTGGTCAGCCTGTATgc                                                                 <  1:1093900/138‑1 (MQ=255)
                                                                                   gggCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTgg                                                         >  2:1578655/1‑140 (MQ=255)
                                                                                    ggCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGt                                                        >  1:1763724/1‑140 (MQ=255)
                                                                                        aaGGCCGCTATTTTTGCGAAAAAATGCCGAAGCAGGCGGGTATTTATAAGTCGCATCCTGCGCTGAAACTCTGTTATGCCTAAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTTTATGCGCATTTGGTccag                                                    <  2:569193/140‑1 (MQ=255)
                                                                                        aaGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTccag                                                    <  2:1604258/140‑1 (MQ=255)
                                                                                         tggCCGCGCTTTTTGCGAAAATCTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGTTATGCCTAAATACATGAAACGAAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTccagc                                                   <  1:1889523/139‑1 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCACAACGACCGTGAAGTGGGCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  2:296114/1‑140 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  1:211018/1‑140 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  1:1485156/1‑140 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  1:1361563/1‑140 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  2:1488740/1‑140 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  2:1489290/1‑140 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  1:1689424/1‑140 (MQ=255)
                                                                                               cgcATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGttt                                             >  1:568798/1‑140 (MQ=255)
                                                                                                gcATTTTGCTAAAAACTGGCGCAGCTGGCGGGTATTGAACAGTCGCATCCGGCGCTGCAACGCTGTTATGCCTAAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGtttt                                            <  1:323517/140‑1 (MQ=255)
                                                                                                  aTTTTGCGAAAAACTGGCGCAGCAGGCGTTAATTGATCAGACGCACCCGGCGCTGCAACGATGGTATGCCTAAATCCATGAAACGCAAAACGTCCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAg                                          <  2:666155/140‑1 (MQ=255)
                                                                                                  aTTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTTTGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCTTTTGGTCCAGCCAGTTTTAg                                          <  2:362386/140‑1 (MQ=255)
                                                                                                  aTTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAg                                          <  2:1788167/140‑1 (MQ=255)
                                                                                                   ttttGCGAAAAACTGGCGCAGCAGGCGGGTATTTATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCACAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGa                                         <  2:1281988/140‑1 (MQ=255)
                                                                                                       gCGAAAAACTGGCGCAGCTGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTTGGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGcc                                     <  1:1294057/140‑1 (MQ=255)
                                                                                                                            cGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGc                >  1:1842623/1‑140 (MQ=255)
                                                                                                                            cGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGc                >  1:869587/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGa  >  1:1401181/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGa  >  1:1779174/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGa  >  1:1197049/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGa  >  1:29678/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGa  >  1:1194700/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGa  >  1:1399955/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGa  >  1:1132420/1‑140 (MQ=255)
                                                                                                                                          tCGCATCCGGCGCTGCAACGCTGGTAAGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGATCGGCGGCGGa  >  1:29536/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ACAGAGTGGCGGCGCTTATGGCGCTTGCGACAGCGCGCCCATGAACGCGACGAAGCGCGTGATTTATTGCATAGCCACGGCACGGGCAAAGGCCGCGCATTTTGCGAAAAACTGGCGCAGCAGGCGGGTATTGATCAGTCGCATCCGGCGCTGCAACGCTGGTATGCCTCAATCCATGAAACGCAAAACGACCGTGAAGTGGTCAGCCTGTATGCGCATTTGGTCCAGCCAGTTTTAGATGCCCAGGCGCGGCGCGAAATCAGCCGTTCGGCGGCGGA  >  NC_000913/1385871‑1386148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑