Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,405,953 C→T 10.2% intergenic (‑202/‑26) mcaS ← / → smrA motility and biofilm regulator sRNA/DNA endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,405,9530CT10.2% 150.1 / 7.0 49intergenic (‑202/‑26)mcaS/smrAmotility and biofilm regulator sRNA/DNA endonuclease
Reads supporting (aligned to +/- strand):  ref base C (29/15);  new base T (3/2);  total (32/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCG  >  NC_000913/1405815‑1406091
                                                                                                                                          |                                                                                                                                          
atatAAGTTACATTTTTAATATCGGGAATGAATAATCATGCATAAGTATTTTGCTTTAAATATCGGCAATATTAGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATcgcg                                                                                                                                           <  2:681122/140‑1 (MQ=255)
             tattaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCgg                                                                                                                              <  1:463777/140‑1 (MQ=255)
               ttaagctCGTGACTGAAGAATCATGCATCAGTATTTTGCTTAAACTAGCGGCAATATATGGAACTTATTCCTGGAAATTTGGGTAAGACGTGGGGGGACCGCCCCGGTCTGGTTATCATATCGCGCTCGTTAGTGCGgga                                                                                                                            <  2:1051380/134‑1 (MQ=255)
               ttaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGgga                                                                                                                            <  1:818245/140‑1 (MQ=255)
               ttaATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGAAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTATGGTTATCATATCGCGCTCTTAATTGCGgga                                                                                                                            <  2:1747759/140‑1 (MQ=255)
                       gTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTaa                                                                                                                    >  1:105361/1‑140 (MQ=255)
                       gTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTaa                                                                                                                    >  1:846442/1‑140 (MQ=255)
                       gTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATAACTGGAAATTTGGGTAAAACGTTTTTGTACCTACCCGGTCTGGTTAACATAACGTCATCTTAATTGCGGGAGGATGTaa                                                                                                                    >  2:409750/1‑140 (MQ=255)
                        tgaatgaaTAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAAc                                                                                                                   >  2:329792/1‑140 (MQ=255)
                         gaatgaatAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACa                                                                                                                  >  2:1661931/1‑140 (MQ=255)
                          aatgaatAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACAt                                                                                                                 >  1:171267/1‑140 (MQ=255)
                             gaatAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGaa                                                                                                              >  2:839916/1‑140 (MQ=255)
                             gaatAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGaa                                                                                                              >  2:1572764/1‑140 (MQ=255)
                                             gTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCg                                                                                              >  2:226862/1‑140 (MQ=255)
                                             gTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCg                                                                                              <  2:743639/140‑1 (MQ=255)
                                             gTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCg                                                                                              >  1:889813/1‑140 (MQ=255)
                                                ttttGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTg                                                                                           >  1:759835/1‑140 (MQ=255)
                                                 tttGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGt                                                                                          >  2:780158/1‑140 (MQ=255)
                                                  ttGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGtt                                                                                         >  1:1278886/1‑140 (MQ=255)
                                                   tGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGGTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAATTCGCTGttt                                                                                        >  2:169822/1‑140 (MQ=255)
                                                     cTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCt                                                                                      >  2:38242/1‑140 (MQ=255)
                                                     cTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACAGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCt                                                                                      >  1:221569/1‑140 (MQ=255)
                                                        aaaaTATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGa                                                                                   <  1:1787321/140‑1 (MQ=255)
                                                        aaaaTATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGa                                                                                   <  1:1410656/140‑1 (MQ=255)
                                                             atCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCa                                                                              <  1:1115310/140‑1 (MQ=255)
                                                             atCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCa                                                                              <  2:1569558/140‑1 (MQ=255)
                                                             atCGGCAATATTTGGAACTTATTACTGGAAATTTGGGAAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCa                                                                              <  2:1569679/140‑1 (MQ=255)
                                                                                 attaCTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGTGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTg                                                          >  1:1875311/1‑140 (MQ=255)
                                                                                 attaCTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGTGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTg                                                          >  1:1875472/1‑140 (MQ=255)
                                                                                              tGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCg                                             <  2:1357240/140‑1 (MQ=255)
                                                                                                          tgttgGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGACGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGc                                 >  1:462926/1‑140 (MQ=255)
                                                                                                          tgttgGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGc                                 >  1:827227/1‑140 (MQ=255)
                                                                                                          tgttgGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGc                                 >  1:824628/1‑140 (MQ=255)
                                                                                                          tgttgGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGc                                 >  1:824491/1‑140 (MQ=255)
                                                                                                          tgttgGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGc                                 >  1:724826/1‑140 (MQ=255)
                                                                                                          tgttgGACCGACCCGGTCTGGTAATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTGTCTTGACGCCATGGAAGATGTCCATCCGCTGAAACGTGCTACCGATGTCCACTGGc                                 >  1:827244/1‑140 (MQ=255)
                                                                                                               gaccgaccCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCg                                             >  2:662791/1‑123 (MQ=255)
                                                                                                               gaccgaccCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCg                                             <  1:662791/123‑1 (MQ=255)
                                                                                                                    accCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAAcgcg                       >  1:1580562/1‑140 (MQ=255)
                                                                                                                    accCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAAcgcg                       >  1:639393/1‑140 (MQ=255)
                                                                                                                    accCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAAcgcg                       >  1:1498910/1‑140 (MQ=255)
                                                                                                                    accCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGACGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCAAGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGACCACTGTCAACCAAcccg                       >  1:1049121/1‑140 (MQ=255)
                                                                                                                         gTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAAcc                  >  2:1609884/1‑140 (MQ=255)
                                                                                                                         gTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAAcc                  >  2:1609537/1‑140 (MQ=255)
                                                                                                                          tCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCa                 <  1:143992/140‑1 (MQ=255)
                                                                                                                                 aTCATATCGTGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTgcg          <  2:1875311/140‑1 (MQ=255)
                                                                                                                                 aTCATATCGTGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTgcg          <  2:1875472/140‑1 (MQ=255)
                                                                                                                                    atatCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGcgccg       >  2:1476799/1‑140 (MQ=255)
                                                                                                                                    atatCGCGCTCTTAAGTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGAGGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGcgccg       <  2:584904/140‑1 (MQ=255)
                                                                                                                                       tCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAg    <  2:151800/140‑1 (MQ=255)
                                                                                                                                         gcgcTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCg  <  2:277450/140‑1 (MQ=255)
                                                                                                                                         gcgcTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCg  <  2:1488932/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ATATAAGTTACATTATTAATATCGTGAATGAATAATCATGCATAAGTATTTTGCTTAAAATATCGGCAATATTTGGAACTTATTACTGGAAATTTGGGTAATACGTTGTTGGACCGACCCGGTCTGGTTATCATATCGCGCTCTTAATTGCGGGAGGATGTAACATGAACCTTGACGACAAATCGCTGTTTCTTGACGCCATGGAAGATGTCCAGCCGCTGAAACGTGCTACCGATGTCCACTGGCATCCAACGCGTAACCAACGTGCGCCGCAGCG  >  NC_000913/1405815‑1406091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑