Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,451,132 G→T 7.5% P73H (CCT→CAT)  tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,451,1320GT 7.5% 176.6 / 3.1 53P73H (CCT→CAT) tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base G (21/28);  new base T (2/2);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

AAAGGTTTGATCCAGCCCGGACTGGAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCTTCACCACCGTGGGC  >  NC_000913/1450999‑1451262
                                                                                                                                     |                                                                                                                                  
caaGGTTTGATCCAGCCCGGACTGGAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCTTCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCa                                                                                                                              <  2:1300164/139‑1 (MQ=255)
               cccTGACTGGAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACgcgc                                                                                                               <  2:860866/140‑1 (MQ=255)
               cccGGACTGTAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACgcgc                                                                                                               <  1:967546/140‑1 (MQ=255)
               cccGGACTGGAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACgcgc                                                                                                               <  1:967601/140‑1 (MQ=255)
               cccGGACTGGAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACgcgc                                                                                                               >  2:393936/1‑140 (MQ=255)
                    aCTGGAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCt                                                                                                          <  1:1504337/140‑1 (MQ=255)
                        gAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTaa                                                                                                      >  2:479558/1‑140 (MQ=255)
                        gAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTaa                                                                                                      >  2:498360/1‑140 (MQ=255)
                         aaaaCATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAAt                                                                                                     <  1:367062/140‑1 (MQ=255)
                         aaaaCATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAAt                                                                                                     <  2:68801/140‑1 (MQ=255)
                         aaaaCATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCAAGTACGCGCCATCTTTAAt                                                                                                     <  1:1012241/140‑1 (MQ=255)
                          aaaCATCGTTAATAAATGTGTCAGAAACCCAGGCTTTATTGTATTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACTATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATc                                                                                                    <  2:1628044/140‑1 (MQ=255)
                          aaaCATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATc                                                                                                    <  2:1240627/140‑1 (MQ=255)
                          aaaCATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATc                                                                                                    <  2:257288/140‑1 (MQ=255)
                          aaaCATCGTTAATAAAGGTGACAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATc                                                                                                    <  2:1498370/140‑1 (MQ=255)
                           aaCATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCa                                                                                                   <  1:1781272/140‑1 (MQ=255)
                              aTCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACttt                                                                                                                          <  1:842872/114‑1 (MQ=255)
                              aTCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACttt                                                                                                                          >  2:842872/1‑114 (MQ=255)
                                       aaaGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCt                                                                                       >  1:1498761/1‑140 (MQ=255)
                                       aaaGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCt                                                                                       >  1:1499016/1‑140 (MQ=255)
                                              tCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCATGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTa                                                                                >  1:831804/1‑140 (MQ=255)
                                              tCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCATGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTa                                                                                >  1:828713/1‑140 (MQ=255)
                                              tCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTTCGCGCCATCTTTAATCAGGGTCAAGAGCTGGGCGTa                                                                                >  1:1521686/1‑140 (MQ=255)
                                                aGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAgt                                                                              <  2:1342868/140‑1 (MQ=255)
                                                  aaaCCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAgtcg                                                                            <  2:158385/140‑1 (MQ=255)
                                                  aaaCCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAgtcg                                                                            <  2:157759/140‑1 (MQ=255)
                                                  aaaCCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAgtcg                                                                            <  2:844862/140‑1 (MQ=255)
                                                     cccAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGTGTAGTCGTcc                                                                         <  1:34553/140‑1 (MQ=255)
                                                     cccAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTcc                                                                         <  2:1266726/140‑1 (MQ=255)
                                                     cccAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTcc                                                                         >  1:784853/1‑140 (MQ=255)
                                                     cccAGGCTTAATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCTCTTTCACGTACTCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTcc                                                                         >  2:619240/1‑140 (MQ=255)
                                                                     tttCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCAc                                                         >  2:1020179/1‑140 (MQ=255)
                                                                        catcaCTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCaa                                                      >  1:1476658/1‑140 (MQ=255)
                                                                                     aCTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGt                                         >  1:425432/1‑140 (MQ=255)
                                                                                       ttGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGttt                                       >  1:229920/1‑140 (MQ=255)
                                                                                              gCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTATGCGTTTTATCCAt                                >  1:1127092/1‑140 (MQ=255)
                                                                                                 agagGCTGACCATTAACAATTGCTGTTTGCGCACCATGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTgg                             <  2:831804/140‑1 (MQ=255)
                                                                                                 agagGCTGACCATTAACAATTGCTGTTTGCGCACCATGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTgg                             <  2:828713/140‑1 (MQ=255)
                                                                                                 agagGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTgg                             <  1:1304555/140‑1 (MQ=255)
                                                                                                  gagGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGc                            <  2:59984/140‑1 (MQ=255)
                                                                                                     gCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCAcc                         <  2:458903/140‑1 (MQ=255)
                                                                                                     gCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCAcc                         <  2:13230/140‑1 (MQ=255)
                                                                                                       tGACCATTAACAATTGCAGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGTTAAAGAGCTGGGCGTACTCGTCCCACTTCACATCGGCACCAAATTCTTTAATCGTTTTATCCATTGGCAACaa                       >  1:1632646/1‑139 (MQ=255)
                                                                                                         acccgtAACAATTGCTGGATGCGCACCAGGCAGCACTTTCACGTACGCGCCATCGGGAAGCAGGGGAAAGAGCGGGCCGGTGTCGTCCCACGGCCCATCGGCACCCACGTCTGAAAGCGTGGTAGCCATTGGCACCatat                     >  2:1791110/6‑140 (MQ=255)
                                                                                                         aCCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAagag                                                                                         <  1:131219/72‑1 (MQ=255)
                                                                                                         aCCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAagag                                                                                         >  2:131219/1‑72 (MQ=255)
                                                                                                          ccATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATg                    <  2:559961/140‑1 (MQ=255)
                                                                                                               aaCAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCtt               >  2:1097766/1‑140 (MQ=255)
                                                                                                                     tGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCTTcaccac         >  2:1548843/1‑140 (MQ=255)
                                                                                                                         gTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCTTCACCACCGTg     <  1:1794956/140‑1 (MQ=255)
                                                                                                                         gTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCTTCACCACCGTg     <  2:1571732/140‑1 (MQ=255)
                                                                                                                          tttGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCTTCACCACCGTgg    >  2:1072592/1‑140 (MQ=255)
                                                                                                                            tGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCTTCACCACCGTGGGc  >  2:176848/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                  
AAAGGTTTGATCCAGCCCGGACTGGAAAACATCGTTAATAAAGGTGTCAGAAACCCAGGCTTTATTGTCTTTCATCACTACCGGTACTTGCAGTGCCAGAGGCTGACCATTAACAATTGCTGTTTGCGCACCAGGCTTCACTTTCACGTACGCGCCATCTTTAATCAGGGTAAAGAGCTGGGCGTAGTCGTCCCACTGCACATCGGCACCAAATTCTTTAAGCGTTTTATCCATTGGCACCATATGCGCTTCACCACCGTGGGC  >  NC_000913/1450999‑1451262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑