Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,476,962 T→C 6.6% W607R (TGG→CGG)  ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,476,9620TC 6.6% 187.6 / 4.6 61W607R (TGG→CGG) ydbDPF10971 family putative periplasmic methylglyoxal resistance protein
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base C (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

ATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTTATTATGC  >  NC_000913/1476825‑1477098
                                                                                                                                         |                                                                                                                                        
aTGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTgg                                                                                                                                        <  1:700576/140‑1 (MQ=255)
  gCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGTAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGa                                                                                                                                      <  2:1417946/140‑1 (MQ=255)
  gCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGa                                                                                                                                      <  1:950085/140‑1 (MQ=255)
    atatGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATa                                                                                                                                    >  2:792184/1‑140 (MQ=255)
     tatGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATaa                                                                                                                                   >  1:757830/1‑140 (MQ=255)
        gAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAAttt                                                                                                                                <  2:337459/140‑1 (MQ=255)
        gAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAAttt                                                                                                                                >  1:1796509/1‑140 (MQ=255)
           aCCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCCGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTAcc                                                                                                                             <  1:898486/140‑1 (MQ=255)
              aGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGc                                                                                                                          >  2:893149/1‑140 (MQ=255)
                cgcTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCtg                                                                                                                        >  1:854586/1‑140 (MQ=255)
                cgcTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCtg                                                                                                                        >  1:177817/1‑140 (MQ=255)
                cgcTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCtg                                                                                                                        >  2:1443442/1‑140 (MQ=255)
                cgcTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAAAGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCTCtg                                                                                                                        >  2:1443415/1‑140 (MQ=255)
                cgatGATATGATTTTGTCTGAGCTGATTAGCCACGGCGTAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATCATTTACCCGCtg                                                                                                                        >  2:1443469/4‑140 (MQ=255)
                 gcTGATATGATTTTGTCTGAGCTGATTAGCCTCGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCtgt                                                                                                                       <  2:179419/140‑1 (MQ=255)
                       atGATTGTGTCTGAGCTGAGAAGCCACGGCGCAGTTGCTGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTtct                                                                                                                 <  2:1918941/140‑1 (MQ=255)
                           ttttGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAg                                                                                                             >  2:1449120/1‑140 (MQ=255)
                               gTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAAt                                                                                                         >  2:522282/1‑140 (MQ=255)
                                 cTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTa                                                                                                       <  1:1874444/140‑1 (MQ=255)
                                              ccACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAAcacgc                                                                                          <  1:976166/140‑1 (MQ=255)
                                                     gAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCaa                                                                                   <  1:421155/140‑1 (MQ=255)
                                                     gAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCaa                                                                                   <  1:999716/140‑1 (MQ=255)
                                                            atgaCCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATAtt                                                                            >  2:694180/1‑140 (MQ=255)
                                                                 cAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGc                                                                       <  1:196123/140‑1 (MQ=255)
                                                                 cAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGc                                                                       <  1:195969/140‑1 (MQ=255)
                                                                   gATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGa                                                                     >  1:1542045/1‑140 (MQ=255)
                                                                   gATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGa                                                                     >  1:1472537/1‑140 (MQ=255)
                                                                   gATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTGTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGa                                                                     >  2:1782133/1‑140 (MQ=255)
                                                                    aTGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACTCGCCACGCAATGAGATTTGGGCGaa                                                                    >  2:854256/1‑140 (MQ=255)
                                                                           tAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGtttttt                                                             >  2:1203813/1‑140 (MQ=255)
                                                                                  cACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGTAGTTTTTTCTGAGGc                                                      >  2:1789508/1‑140 (MQ=255)
                                                                                  cACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGc                                                      >  1:21635/1‑140 (MQ=255)
                                                                                     cGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAgg                                                   <  2:1116078/140‑1 (MQ=255)
                                                                                     cGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAgg                                                   <  2:1597354/140‑1 (MQ=255)
                                                                                            aTACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAg                                            <  2:1180039/140‑1 (MQ=255)
                                                                                            aTACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAg                                            >  1:1701889/1‑140 (MQ=255)
                                                                                             tACGTTCGGACGAGTGGGACTTTTTTGAGAGTGCAGTATTTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACTCGCCACGCACGGATATTTGGGCGTAGTTTTTTCTGAGGCAGGTAATCAGt                                           >  2:1244428/1‑140 (MQ=255)
                                                                                                 ttCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTccc                                       >  1:116427/1‑140 (MQ=255)
                                                                                                  tCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCg                                      >  1:1576024/1‑140 (MQ=255)
                                                                                                      acgacTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTgcg                                  <  1:1337285/140‑1 (MQ=255)
                                                                                                          cTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAgg                              <  1:957086/140‑1 (MQ=255)
                                                                                                                cTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGGTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACg                        <  1:1306776/140‑1 (MQ=255)
                                                                                                                 tttttAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGa                       <  1:1449120/140‑1 (MQ=255)
                                                                                                                   tttAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAg                     >  1:1470450/1‑140 (MQ=255)
                                                                                                                    ttAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGc                    <  1:572646/140‑1 (MQ=255)
                                                                                                                        agagTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTa                <  1:792184/140‑1 (MQ=255)
                                                                                                                           gTGCATTAATTAGTCGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACgt             >  1:1815273/1‑140 (MQ=255)
                                                                                                                           gTGCATTAATTAGTCGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACgt             >  1:1815264/1‑140 (MQ=255)
                                                                                                                            tGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACgtg            >  1:318833/1‑140 (MQ=255)
                                                                                                                            tGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACgtg            >  1:1148832/1‑140 (MQ=255)
                                                                                                                            tGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACgtg            >  1:1132511/1‑140 (MQ=255)
                                                                                                                             gCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACgtgt           <  1:1414698/140‑1 (MQ=255)
                                                                                                                              cATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGtt          >  2:299436/1‑140 (MQ=255)
                                                                                                                              cATTAATTAGTCGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGtt          <  2:1815264/140‑1 (MQ=255)
                                                                                                                              cATTAATTAGTCGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGtt          <  2:1815273/140‑1 (MQ=255)
                                                                                                                                    ttaGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTtattat    <  2:177817/140‑1 (MQ=255)
                                                                                                                                    ttaGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTtattat    <  2:854586/140‑1 (MQ=255)
                                                                                                                                     taGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTTATTATg   <  1:916443/140‑1 (MQ=255)
                                                                                                                                     taGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTTATTATg   <  1:893149/140‑1 (MQ=255)
                                                                                                                                     taGTTGGGATAATTTACCCGCTGGTGTTCACCAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTTATTATg   <  1:494488/140‑1 (MQ=255)
                                                                                                                                      aGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTTATTATGc  >  1:142285/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCTGTTGTTCTCAAGGAATTACAGCAAAACACGCCACGCAATGATATTTGGGCGAAGTTTTTTCTGAGGCAGGAAAACAGTTCCCGTGCGCAGGTTGACGAAGCGTTACGTGTTTATTATGC  >  NC_000913/1476825‑1477098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: