Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,498,458 T→C 5.1% F535L (TTC→CTC)  opgD → OPG biosynthetic periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,498,4580TC 5.1% 243.3 / 4.1 79F535L (TTC→CTC) opgDOPG biosynthetic periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (34/39);  new base C (2/2);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

AACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTA  >  NC_000913/1498330‑1498596
                                                                                                                                |                                                                                                                                          
caCCCATCGAAGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 <  1:458723/139‑1 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 <  1:555893/140‑1 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 >  2:421392/1‑140 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 >  2:574884/1‑140 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 <  1:1858030/140‑1 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 <  1:426307/140‑1 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 >  2:574902/1‑140 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 <  1:391170/140‑1 (MQ=255)
aaCCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTAACAGTGAAACATGGCTGTATCAGTATTTCCCGCCAgcg                                                                                                                                 <  1:1858237/140‑1 (MQ=255)
     atagATGTTTATCGTATTCAGTTTGACTGGTATCCGACTTAGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGTGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                                                                                                            <  2:17091/137‑1 (MQ=255)
     aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTTAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                                                                                                            >  2:469801/1‑140 (MQ=255)
     aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                                                                                                            >  1:809847/1‑140 (MQ=255)
     aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                                                                                                            <  2:202907/140‑1 (MQ=255)
     aTCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGa                                                                                                                            >  2:643740/1‑140 (MQ=255)
        gacggTTATCCTCTTCAGTTTGACTGCTATCCGACTTCGGACCCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGAGATACATGGCTGTATCAGTATGTCCCGCCAGCGCCGGAtta                                                                                                                         <  2:1624040/137‑3 (MQ=255)
         aTGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATaaa                                                                                                                        <  1:663236/140‑1 (MQ=255)
             ttATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTc                                                                                                                    <  2:221853/140‑1 (MQ=255)
              tATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCa                                                                                                                   <  2:396185/140‑1 (MQ=255)
               aTCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGAAATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAg                                                                                                                  >  2:1550155/1‑140 (MQ=255)
                tCGTATTCAGTTTGACTGGTATCCGAGTTCGGACTACACTGATCTGGTCGATATGCGGATGTTTCTACGTTGTCAGGGGGACTCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAATCCTCAGt                                                                                                                 <  2:240467/140‑1 (MQ=255)
                tCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 <  2:592028/140‑1 (MQ=255)
                tCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 <  1:803321/140‑1 (MQ=255)
                tCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 >  1:1542125/1‑140 (MQ=255)
                tCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 <  1:801096/140‑1 (MQ=255)
                acGTAGTCAGATTGACTGGAATCCGCCTTCGGCCACCAGTGATCCGGTCGATATGCGGATGTCTCTACGTTGTCAGGGGGACGCTATCACTGAAACATGGCTGTATCCGTATTTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 <  2:184336/139‑1 (MQ=255)
                     ttCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGtt                                                                                                            >  1:63631/1‑140 (MQ=255)
                          tttGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTgacga                                                                                                       >  2:254469/1‑140 (MQ=255)
                           ttGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTgacgac                                                                                                      <  1:643740/140‑1 (MQ=255)
                              aCTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACcgc                                                                                                   >  1:984331/1‑140 (MQ=255)
                                  gTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTCTCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGtga                                                                                               >  1:1903440/1‑140 (MQ=255)
                                  gTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGtga                                                                                               >  1:916329/1‑140 (MQ=255)
                                                 cTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGtttt                                                                                <  2:649291/140‑1 (MQ=255)
                                                         aTCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCgg                                                                        >  2:1488250/1‑140 (MQ=255)
                                                            cGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCAc                                                                     <  2:767384/140‑1 (MQ=255)
                                                             gggcGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAACCATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCAcc                                                                    <  1:83642/137‑1 (MQ=255)
                                                              gTCGATATGCGGCTGTCTCTACGTTGTCCGGGGGACGCTATCAGTGAAACATGGCTGTCTCAGTATTTCCCGACAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   <  2:153958/140‑1 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   <  1:833134/140‑1 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   >  1:598970/1‑140 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   >  2:357559/1‑140 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   >  1:395053/1‑140 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   <  2:1218029/140‑1 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   >  1:1869032/1‑140 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACct                                                                   >  2:597594/1‑140 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTAAGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCAGCACct                                                                   >  2:1275992/1‑140 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATCTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 >  2:465252/1‑94 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATCTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 >  2:465208/1‑94 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATCTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 <  1:465208/94‑1 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATCTCCCGCCAGCGCCGGATAAACGTCAGt                                                                                                                 <  1:465252/94‑1 (MQ=255)
                                                              gTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGGATCAGTATTTCCCGCCCGCGCCGGCTAAACGTCAGTATGTTGACGACCGCGTGATGAGTGATTCGTTTTTTCTTCGGCACct                                                                   >  1:1006040/1‑140 (MQ=255)
                                                                 gATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACcttct                                                                <  2:611606/140‑1 (MQ=255)
                                                                 gATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACcttct                                                                <  2:611544/140‑1 (MQ=255)
                                                                        ggATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAg                                                         >  1:282930/1‑140 (MQ=255)
                                                                               tCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTACTTCGGGAGGTGCCGt                                                  >  2:1419336/1‑140 (MQ=255)
                                                                                     gtGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGtt                                            <  1:171856/139‑1 (MQ=255)
                                                                                       gTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTaa                                          >  1:179378/1‑140 (MQ=255)
                                                                                       gTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTATTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTaa                                          >  2:550164/1‑140 (MQ=255)
                                                                                          aGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCTCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAaca                                       <  2:1238958/140‑1 (MQ=255)
                                                                                          aGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCTCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAaca                                       <  2:383087/140‑1 (MQ=255)
                                                                                                      tCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCg                           >  1:1684852/1‑140 (MQ=255)
                                                                                                         gTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCAt                        >  1:510501/1‑140 (MQ=255)
                                                                                                          tGAAACATGGGTGTCTCAGTATTTCCCGACAGCGCCGGATAAACGACAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCAtt                       <  2:4262/140‑1 (MQ=255)
                                                                                                          tGAAACATGGCTGTATCAGTTTTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCAGTCTGGTTAAACACGATCCCGCTCGCAtt                       <  2:4216/140‑1 (MQ=255)
                                                                                                          tGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCAtt                       <  2:4127/140‑1 (MQ=255)
                                                                                                          cgatccATGGCTGTATCAGATTGTCCCGCCAGCGCCGGATAATCGTCAGTAGGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAATCACGATCCCGCTCGCAtt                       <  2:4220/135‑1 (MQ=255)
                                                                                                            aaaCATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCAtttt                     >  1:476759/1‑140 (MQ=255)
                                                                                                               cATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTcc                  <  2:598970/140‑1 (MQ=255)
                                                                                                                aTGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTccc                 >  2:585921/1‑140 (MQ=255)
                                                                                                                  ggCTGTATCAGTATTTCCCGCCAGCGCCGGATATACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTa               <  2:1141853/140‑1 (MQ=255)
                                                                                                                  ggCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTa               >  2:1471915/1‑140 (MQ=255)
                                                                                                                  ggCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTa               >  1:489453/1‑140 (MQ=255)
                                                                                                                    cTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAg             >  1:1884708/1‑140 (MQ=255)
                                                                                                                     tGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTATTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGt            <  2:1463628/140‑1 (MQ=255)
                                                                                                                       tATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTa          <  1:1039913/140‑1 (MQ=255)
                                                                                                                          cAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAAt       >  1:712908/1‑140 (MQ=255)
                                                                                                                             tATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGTGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGCTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAg    <  2:1409443/140‑1 (MQ=255)
                                                                                                                             tATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAg    <  1:465216/140‑1 (MQ=255)
                                                                                                                             tATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAg    <  1:465229/140‑1 (MQ=255)
                                                                                                                             tATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAg    <  2:1778689/140‑1 (MQ=255)
                                                                                                                              aTTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGt   >  1:68493/1‑140 (MQ=255)
                                                                                                                               tttCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTa  >  2:438688/1‑140 (MQ=255)
                                                                                                                               tttCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTa  <  2:1402462/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                          
AACCCATCGATGGTTATCGTATTCAGTTTGACTGGTATCCGACTTCGGACTCCACTGATCCGGTCGATATGCGGATGTATCTACGTTGTCAGGGGGACGCTATCAGTGAAACATGGCTGTATCAGTATTTCCCGCCAGCGCCGGATAAACGTCAGTATGTTGACGACCGCGTGATGAGTTAATCGTTTTTTCTTCGGCACCTTCTTCGGGAGGTGCCGTCTGGTTAAACACGATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTA  >  NC_000913/1498330‑1498596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: