| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,610,940 | G→T | 7.9% | T305T (ACC→ACA) | yneF ← | putative membrane‑bound diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,610,940 | 0 | G | T | 7.9% | 227.7 / 2.2 | 64 | T305T (ACC→ACA) | yneF | putative membrane‑bound diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base G (24/34); new base T (2/4); total (26/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCAATACTTACCGTGAGATAAAGC > NC_000913/1610803‑1611075 | aaaGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGt > 2:1106478/1‑140 (MQ=255)aaaGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGt < 2:1550169/140‑1 (MQ=255)aaaGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGt < 2:619208/140‑1 (MQ=255) aaTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGa > 1:713769/1‑140 (MQ=255) aaTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGa > 1:482683/1‑140 (MQ=255) aaTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGa < 1:1923048/140‑1 (MQ=255) atagttgAATAACGGATTGTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATTTATATAGATGTCAGACTAATTCCTAACCGTAACGCATAGTTCTGGTACGGTTGCGCCCATCTTTATTCGAg < 2:35852/134‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACTTTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTTGTACGGTTGCGCCCATCTTTCTTCGAg < 2:939876/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTTGTACGGTTGCGCCCATCTTTCTTCGAg < 2:657436/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTTGTACGGTTGCGCCCATCTTTCTTCGAg < 2:657495/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 1:1047298/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 1:617563/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 2:1381790/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 1:1045960/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 2:296355/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 1:723043/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 1:1911171/140‑1 (MQ=255) aTCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAg < 1:1743204/140‑1 (MQ=255) cGATGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCg < 1:161564/140‑1 (MQ=255) cGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCATACAACTTCCTCAACTTAAATAATATTGATTGTACGGTTGCGCCAAtattttttggttgt < 1:798786/140‑7 (MQ=255) cGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCg < 2:1303663/140‑1 (MQ=255) gAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGa < 2:588813/140‑1 (MQ=255) aGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGAt > 1:1558135/1‑140 (MQ=255) aCGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGaca < 2:1163670/140‑1 (MQ=255) aCGCCTTTTCCTGGAGCCTAAAGTGGTCGAACACAGTGCATTCACCACGCGCGTACTCAATAAAGATGTCAGACAACGTCCTCCCGGTAACGCATAGTGCTGGTTCGGTTGCGCCCATCTTTCTTCGAGCGAAACAGaca < 2:822725/140‑1 (MQ=255) gATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACCTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCg > 1:942990/1‑140 (MQ=255) tAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCAt > 1:1195806/1‑140 (MQ=255) tAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCAt > 1:1389430/1‑140 (MQ=255) tAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGAAACGCATAGTGCTTGTAAGGTTGCGCCCATCTTTCTTCGAGCGATACAGAAACGTATCGGCTTCTACCaa > 2:606333/1‑139 (MQ=255) aaGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCa > 2:1637580/1‑140 (MQ=255) aaGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCa < 1:1701871/140‑1 (MQ=255) tGGTAGAACACATTGCATACAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCTTAACGCATAGTGCAGGTACTGTTTCACCAATCTTTCTACGATCGAAACAAACACGTAACTGCTTCGACCaaaaaat > 2:1882276/1‑134 (MQ=255) gTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATtta > 1:603888/1‑140 (MQ=255) aaCACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTaa < 1:1424617/140‑1 (MQ=255) aaCACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTaa < 1:1012147/140‑1 (MQ=255) aaCACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTATTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTaa < 1:929409/140‑1 (MQ=255) caTTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTc > 2:17460/1‑140 (MQ=255) tGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATc > 1:1028545/1‑140 (MQ=255) tGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATc > 1:1349730/1‑140 (MQ=255) aTTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGt < 1:251311/140‑1 (MQ=255) aTTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGt < 1:251522/140‑1 (MQ=255) aTCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGt < 2:374765/140‑1 (MQ=255) aTCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGt < 2:376234/140‑1 (MQ=255) gctcgTAATGAATAAAGATGTCAGAAAACTTCCTCACCGTAACGCATAGTGCTTGAACTGTTGCTCCCATCTTTCTACGAGCGATACAGACACGTATCGGCTTCTACCATCAATATATTAAAGTCATCGGTCAGCGTTAg > 2:1850160/4‑140 (MQ=255) gcgcgTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAAATTATTAAAGTCATCGGTCAGCGTTAg > 2:1850619/1‑140 (MQ=255) cgTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTa < 1:619903/140‑1 (MQ=255) aaGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGAc > 2:1813626/1‑140 (MQ=255) aaGATGTCAGACAACGTCCTCACCGTACCGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGAc < 2:1589907/140‑1 (MQ=255) tCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAc > 2:1332450/1‑140 (MQ=255) tCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAc > 2:1478859/1‑140 (MQ=255) tCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAc > 2:819313/1‑140 (MQ=255) tCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAc > 1:32227/1‑140 (MQ=255) tCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAc > 2:505667/1‑140 (MQ=255) tCAGACAACTTCCTCACCGTAACGCATAGTGCTGCTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAc > 2:1110389/1‑140 (MQ=255) cAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAcc < 1:403028/140‑1 (MQ=255) cAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAcc < 1:405358/140‑1 (MQ=255) cAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAcc < 1:546612/140‑1 (MQ=255) cAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTAcc < 1:549573/140‑1 (MQ=255) aCTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGcc < 2:482683/140‑1 (MQ=255) aCTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGcc < 1:1894409/140‑1 (MQ=255) cTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCa > 1:1166061/1‑140 (MQ=255) tCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCAATACt < 1:808302/140‑1 (MQ=255) aGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCAATACTTACCGTGAGATaaa > 1:246477/1‑140 (MQ=255) tGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCAATACTTACCGTGAGATAAAGc > 1:980068/1‑140 (MQ=255) | AAAGTGTGGCGCGGATCATGGTTTAATCGAGGAAAAACGCCTTTTCCTGGATCATAAAGTGGTAGAACACATTGCATTCAAATCGCGCGTAATGAATAAAGATGTCAGACAACTTCCTCACCGTAACGCATAGTGCTGGTACGGTTGCGCCCATCTTTCTTCGAGCGATACAGACACGTATCGGCTTCGACCATCAATTTATTAAAGTCATCGGTCAGCGTTAGGTACGATGCGCGACCACTACCGACGCCAATACTTACCGTGAGATAAAGC > NC_000913/1610803‑1611075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |