Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,689,399 C→T 6.4% A275V (GCG→GTG)  manA → mannose‑6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,689,3990CT 6.4% 209.3 / 4.2 62A275V (GCG→GTG) manAmannose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (24/34);  new base T (2/2);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATT  >  NC_000913/1689266‑1689536
                                                                                                                                     |                                                                                                                                         
ttCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCt                                                                                                                                     <  1:1215922/140‑1 (MQ=255)
  ctcCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGa                                                                                                                                   <  1:930334/140‑1 (MQ=255)
  ctcCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGa                                                                                                                                   <  1:1515502/140‑1 (MQ=255)
  ctcCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGa                                                                                                                                   <  1:1508357/140‑1 (MQ=255)
  ctcCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGa                                                                                                                                   <  1:1385578/140‑1 (MQ=255)
  ctcCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGa                                                                                                                                   <  1:1385583/140‑1 (MQ=255)
     cccGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGc                                                                                                                                >  1:817525/1‑140 (MQ=255)
     cccGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGc                                                                                                                                >  1:1714429/1‑140 (MQ=255)
       cGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCt                                                                                                                              <  2:644975/140‑1 (MQ=255)
        gCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTa                                                                                                                             >  2:635703/1‑140 (MQ=255)
        gCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTa                                                                                                                             >  1:1519557/1‑140 (MQ=255)
        gCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTa                                                                                                                             >  1:1551600/1‑140 (MQ=255)
          tATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTaaa                                                                                                                           >  2:765782/1‑140 (MQ=255)
          tATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTaaa                                                                                                                           <  1:1715654/140‑1 (MQ=255)
          tATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTaaa                                                                                                                           >  2:51204/1‑140 (MQ=255)
            ttGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATa                                                                                                                         <  1:590149/140‑1 (MQ=255)
            ttGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATa                                                                                                                         <  1:1517530/140‑1 (MQ=255)
               cTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGATGTGATGGCAAAATCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACAt                                                                                                                      <  2:788794/140‑1 (MQ=255)
                  aattttgtgAAATAGAACACTGGCGAAGCGTTGTTCCTGTTCGCTGAAACACCGCACGCTTACCTTCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATTACGTGCTGCGTGCGTGTCTGACGACTAAATACATTGa                                                                                                                   <  2:1832982/134‑1 (MQ=255)
                  aaTGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCGTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGa                                                                                                                   <  2:913466/140‑1 (MQ=255)
                         tgAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCg                                                                                                            >  1:1751625/1‑140 (MQ=255)
                              ttGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACt                                                                                                       >  2:323682/1‑140 (MQ=255)
                              ttGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACt                                                                                                       >  2:843900/1‑140 (MQ=255)
                                 aaCCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGt                                                                                                    >  2:1117561/1‑140 (MQ=255)
                                      tGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCa                                                                                               <  2:825294/140‑1 (MQ=255)
                                      tGGCGAAGCGATGGTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCa                                                                                               <  2:825673/140‑1 (MQ=255)
                                                    tCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGaa                                                                                 <  1:176900/140‑1 (MQ=255)
                                                    tCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGaa                                                                                 <  1:1790825/140‑1 (MQ=255)
                                                     ccTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAg                                                                                >  2:1521896/1‑140 (MQ=255)
                                                        gTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGTGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCa                                                                             >  1:1401492/1‑140 (MQ=255)
                                                        gTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGTGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCa                                                                             >  1:1401066/1‑140 (MQ=255)
                                                         ttCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCaa                                                                            <  1:1259983/140‑1 (MQ=255)
                                                              tGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCGGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATCCCGTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCgg                                                                       <  2:1246607/140‑1 (MQ=255)
                                                               gAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATTACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGc                                                                      <  1:1426973/140‑1 (MQ=255)
                                                               gAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGc                                                                      <  1:1426287/140‑1 (MQ=255)
                                                               gAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGc                                                                      <  1:1240072/140‑1 (MQ=255)
                                                                   cacCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAAc                                                                  <  1:258116/140‑1 (MQ=255)
                                                                        cACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGACCTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAgtt                                                             >  2:34418/1‑140 (MQ=255)
                                                                        cACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAgtt                                                             >  2:1368674/1‑140 (MQ=255)
                                                                               aCCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGAcc                                                      >  2:185413/1‑140 (MQ=255)
                                                                               aCCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGAcc                                                      >  1:713039/1‑140 (MQ=255)
                                                                               aCCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGAcc                                                      >  2:1346134/1‑140 (MQ=255)
                                                                                     aaGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCg                                                >  2:1166919/1‑140 (MQ=255)
                                                                                       ggCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGt                                              >  1:142546/1‑140 (MQ=255)
                                                                                        gCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTg                                             <  2:354444/140‑1 (MQ=255)
                                                                                           tGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGaaa                                          >  2:871029/1‑140 (MQ=255)
                                                                                             gcgcTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACa                                        <  1:661847/140‑1 (MQ=255)
                                                                                             gcgcTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACa                                        <  1:416034/140‑1 (MQ=255)
                                                                                             gcgcTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACa                                        <  1:902388/140‑1 (MQ=255)
                                                                                             gcgcTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACa                                        <  1:662732/140‑1 (MQ=255)
                                                                                              cgcTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTTCATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACaa                                       <  1:670416/140‑1 (MQ=255)
                                                                                                  ggAAGTGATGGCAAACTCCGATAACGTGCTGCGTGTGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTg                                   <  2:1401492/140‑1 (MQ=255)
                                                                                                  ggAAGTGATGGCAAACTCCGATAACGTGCTGCGTGTGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTg                                   <  2:1401066/140‑1 (MQ=255)
                                                                                                  ggAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTg                                   <  1:328032/140‑1 (MQ=255)
                                                                                                  ggAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTg                                   <  1:1210797/140‑1 (MQ=255)
                                                                                                   gAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGc                                  >  1:716138/1‑140 (MQ=255)
                                                                                                   gAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGc                                  <  1:1713732/140‑1 (MQ=255)
                                                                                                   gAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGc                                  <  1:1214924/140‑1 (MQ=255)
                                                                                                           ggCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTgg                          >  1:825359/1‑140 (MQ=255)
                                                                                                           ggCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTgg                          >  1:1159401/1‑140 (MQ=255)
                                                                                                                             gctgcGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTgg        <  1:1267803/140‑1 (MQ=255)
                                                                                                                              ctgcGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGga       >  1:1659346/1‑140 (MQ=255)
                                                                                                                                   tgcgGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtt  >  1:735375/1‑140 (MQ=255)
                                                                                                                                   tgcgGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtt  >  1:289296/1‑140 (MQ=255)
                                                                                                                                   tgcgGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGAtt  >  1:1589956/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                         
TTCTCCCCGCTATTGCTGAATGTGGTGAAATTGAACCCTGGCGAAGCGATGTTCCTGTTCGCTGAAACACCGCACGCTTACCTGCAAGGCGTGGCGCTGGAAGTGATGGCAAACTCCGATAACGTGCTGCGTGCGGGTCTGACGCCTAAATACATTGATATTCCGGAACTGGTTGCCAATGTGAAATTCGAAGCCAAACCGGCTAACCAGTTGTTGACCCAGCCGGTGAAACAAGGTGCAGAACTGGACTTCCCGATTCCAGTGGATGATT  >  NC_000913/1689266‑1689536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑