Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,941,612 G→T 5.3% V8V (GTC→GTA mepM ← murein DD‑endopeptidase, space‑maker hydrolase, septation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,941,6120GT 5.3% 299.9 / 2.5 83V8V (GTC→GTAmepMmurein DD‑endopeptidase, space‑maker hydrolase, septation protein
Reads supporting (aligned to +/- strand):  ref base G (46/32);  new base T (2/3);  total (48/35)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCC  >  NC_000913/1941476‑1941740
                                                                                                                                        |                                                                                                                                
gCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACa                                                                                                                               >  1:1763494/1‑140 (MQ=255)
   cAATGGTTTTGACACTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTACGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACagag                                                                                                                            <  1:1374576/140‑1 (MQ=255)
   cAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACagag                                                                                                                            <  1:660205/140‑1 (MQ=255)
     aTGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCg                                                                                                                          >  1:616214/1‑140 (MQ=255)
     aTGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCg                                                                                                                          >  1:616100/1‑140 (MQ=255)
     aTGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCg                                                                                                                          >  1:955819/1‑140 (MQ=255)
     aTGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCg                                                                                                                          >  1:1839012/1‑140 (MQ=255)
         ttttGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCt                                                                                                                      <  2:777521/140‑1 (MQ=255)
           ttGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACTGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCTACAGAGCGGGCTAt                                                                                                                    >  1:1340262/1‑140 (MQ=255)
           ttGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACTGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCTACAGAGCGGGCTAt                                                                                                                    >  1:1336694/1‑140 (MQ=255)
               cAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGt                                                                                                                <  2:418540/140‑1 (MQ=255)
                 aTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTg                                                                                                              <  2:22090/140‑1 (MQ=255)
                     gCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACg                                                                                                          >  2:500751/1‑140 (MQ=255)
                          gCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCTACAGAGCGGGCTATCTGTTGCACGTAATc                                                                                                     <  2:106671/140‑1 (MQ=255)
                          gCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATc                                                                                                     >  1:707413/1‑140 (MQ=255)
                            aTCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCAc                                                                                                   >  2:242208/1‑140 (MQ=255)
                                cGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTcc                                                                                               <  1:902161/140‑1 (MQ=255)
                                cGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTcc                                                                                               <  1:902312/140‑1 (MQ=255)
                                     aTAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAATTTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCAtt                                                                                          >  1:1617911/1‑140 (MQ=255)
                                        aaCATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAt                                                                                       <  1:1412367/140‑1 (MQ=255)
                                        aaCATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAt                                                                                       <  1:1253327/140‑1 (MQ=255)
                                         aCATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGAGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTTAtc                                                                                      <  2:493004/140‑1 (MQ=255)
                                         aCATAAGGCCGCCAGAAAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAtc                                                                                      <  2:268976/140‑1 (MQ=255)
                                           aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAtctc                                                                                    <  1:1719663/140‑1 (MQ=255)
                                           aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAtctc                                                                                    <  1:918941/140‑1 (MQ=255)
                                           aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAtctc                                                                                    >  2:1521332/1‑140 (MQ=255)
                                             aaGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCt                                                                                  >  2:1070886/1‑140 (MQ=255)
                                               tgccgccAGACAGCGACGGCCAGAGTAAGAACGTTGAGCGACCCCAACATAACGCGGTGTCGACGCGGTAAAATATTAAACGCCAGGGCGACAGAGCGGGCTATCTGATGCACGTAATCACTACCTCATAAATCTCCttt                                                                                >  2:762862/2‑140 (MQ=255)
                                                   gccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAgg                                                                            <  2:479386/140‑1 (MQ=255)
                                                    ccAGACAGCGTCGGCCAGAGTAAGAACGGTGAGCGACCCCTACATAACGGGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCTGGc                                                                           <  2:1043855/140‑1 (MQ=255)
                                                    ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTACACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                           <  1:1666433/140‑1 (MQ=255)
                                                    ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                           <  2:93765/140‑1 (MQ=255)
                                                    ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                           <  1:1442519/140‑1 (MQ=255)
                                                    ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                           >  1:531406/1‑140 (MQ=255)
                                                    ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                           >  1:531763/1‑140 (MQ=255)
                                                    ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGCCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                           >  1:531322/1‑140 (MQ=255)
                                                          aGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCg                                                                     >  1:206728/1‑140 (MQ=255)
                                                            cGACGGCCAGAGTAAGGACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGTCAGAGCGGGCTATCTGGTGCAGGTAATCACGGCCTCATTAATCTCCTTTCAGGCCGCTCGct                                                                   >  2:368484/1‑139 (MQ=255)
                                                              aCGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATa                                                                 >  2:214416/1‑140 (MQ=255)
                                                                  ccAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTgg                                                             <  2:573480/140‑1 (MQ=255)
                                                                           ggaCGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAt                                                    >  2:712791/3‑140 (MQ=255)
                                                                           gAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAt                                                    >  2:141576/1‑140 (MQ=255)
                                                                           gAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAt                                                    >  2:835474/1‑140 (MQ=255)
                                                                            aaCGGTGAGCGACCCCAACATAACGCGGTTTTGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAtt                                                   <  2:1442579/140‑1 (MQ=255)
                                                                            aaCGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAtt                                                   >  2:634751/1‑140 (MQ=255)
                                                                            aaCGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAtt                                                   <  2:1444993/140‑1 (MQ=255)
                                                                            aaCGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAtt                                                   >  1:1715251/1‑140 (MQ=255)
                                                                            aaCGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAtt                                                   >  2:634657/1‑140 (MQ=255)
                                                                            aaCGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAAtt                                                   >  2:92199/1‑140 (MQ=255)
                                                                              cGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGa                                                 <  2:1884117/140‑1 (MQ=255)
                                                                                gtgtGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCTCGTTATGAGTTCGTCTTTATTCTCCTTTCAGGCTGCTCGCATACTGGTTGGGTTATTgtgt                                               >  2:1404086/1‑137 (MQ=255)
                                                                                gTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACt                                               >  1:12490/1‑140 (MQ=255)
                                                                                        ccccAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAAtt                                       <  2:136820/140‑1 (MQ=255)
                                                                                        ccccAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAAtt                                       <  2:113983/140‑1 (MQ=255)
                                                                                           cAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTg                                    >  1:1420875/1‑140 (MQ=255)
                                                                                            aaCCTAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGa                                   <  1:1288717/140‑1 (MQ=255)
                                                                                            aaCATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGa                                   <  1:1016436/140‑1 (MQ=255)
                                                                                                 aaCGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGc                              <  1:1204212/140‑1 (MQ=255)
                                                                                                 aaCGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGc                              <  1:1204112/140‑1 (MQ=255)
                                                                                                 aaCGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGc                              <  1:426034/140‑1 (MQ=255)
                                                                                                  aCGCGGTGTGGTAGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCt                             >  1:171627/1‑140 (MQ=255)
                                                                                                  aCGCGGTGTGGTAGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCt                             >  1:173327/1‑140 (MQ=255)
                                                                                                   cgcgGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCtt                            >  2:105438/1‑140 (MQ=255)
                                                                                                   cgcgGTGTGGTCGCGGTAAATAATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCtt                            >  2:1117355/1‑140 (MQ=255)
                                                                                                     cgGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAACTCCTTTCAGGCAGCTCGAATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGt                          >  1:929280/1‑140 (MQ=255)
                                                                                                      ggTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGtt                         >  2:171698/1‑140 (MQ=255)
                                                                                                      ggTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGtt                         >  2:171982/1‑140 (MQ=255)
                                                                                                        tgtgGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGtttt                       >  1:1856211/1‑140 (MQ=255)
                                                                                                          tgGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTCATTGACTCAGGAATTCTGAATAGCTTGTTTTAc                     >  1:514951/1‑140 (MQ=255)
                                                                                                           ggTCGCGGTAAATTATTAAACGCCAGGGCTACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTAcc                    <  2:1340262/140‑1 (MQ=255)
                                                                                                           ggTCGCGGTAAATTATTAAACGCCAGGGCTACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTAcc                    <  2:1336694/140‑1 (MQ=255)
                                                                                                           ggTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTAcc                    <  2:1647515/140‑1 (MQ=255)
                                                                                                           ggTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTAcc                    <  2:330192/140‑1 (MQ=255)
                                                                                                             tCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCa                  >  1:754418/1‑140 (MQ=255)
                                                                                                                cgGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGtt               <  2:861844/140‑1 (MQ=255)
                                                                                                                  gTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTg             >  2:1598139/1‑140 (MQ=255)
                                                                                                                  gTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTg             >  1:892406/1‑140 (MQ=255)
                                                                                                                  gTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTg             >  1:892049/1‑140 (MQ=255)
                                                                                                                      attattAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGatat         >  1:1476759/1‑140 (MQ=255)
                                                                                                                      agtattaAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGatat         >  1:1476970/3‑140 (MQ=255)
                                                                                                                             aaCGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTccc  >  2:191629/1‑140 (MQ=255)
                                                                                                                             aaCGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTccc  >  2:1325445/1‑140 (MQ=255)
                                                                                                                             aaCGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTccc  >  2:7902/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                
GCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCC  >  NC_000913/1941476‑1941740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: