Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,013,898 G→T 5.5% G224W (GGG→TGG)  fliF → flagellar basal‑body MS‑ring and collar protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,013,8980GT 5.5% 271.0 / 2.6 72G224W (GGG→TGG) fliFflagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base G (38/30);  new base T (2/2);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTC  >  NC_000913/2013760‑2014035
                                                                                                                                          |                                                                                                                                         
ttACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCgg                                                                                                                                          >  2:1374171/1‑140 (MQ=255)
ttACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCgg                                                                                                                                          >  2:1258579/1‑140 (MQ=255)
   cccGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGgcg                                                                                                                                       <  1:1256756/140‑1 (MQ=255)
      ggCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGa                                                                                                                                    >  2:255778/1‑140 (MQ=255)
      ggCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGa                                                                                                                                    >  1:1587917/1‑140 (MQ=255)
       gCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGAt                                                                                                                                   >  1:880543/1‑140 (MQ=255)
                 cGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACg                                                                                                                         <  1:1597376/140‑1 (MQ=255)
                 cGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACg                                                                                                                         <  1:1597217/140‑1 (MQ=255)
                      atgggCAATTTTGCGCCTTTGTGCATCTGGATTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAATCGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAAGACCAGCGGGCGCGATCTTAATGACGCTCAg                                                                                                                    <  2:1490867/138‑1 (MQ=255)
                          gCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGa                                                                                                                >  2:913639/1‑140 (MQ=255)
                          gCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGa                                                                                                                >  2:461036/1‑140 (MQ=255)
                            aaaTTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGaaa                                                                                                              >  1:270878/1‑140 (MQ=255)
                            aaaTTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGaaa                                                                                                              >  2:320551/1‑140 (MQ=255)
                            aaaTTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGaaa                                                                                                              <  2:904938/140‑1 (MQ=255)
                            aaaTTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGaaa                                                                                                              >  1:131298/1‑140 (MQ=255)
                                    gcCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAg                                                                                                      <  2:384305/140‑1 (MQ=255)
                                      cATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCTATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCg                                                                                                    <  1:193555/140‑1 (MQ=255)
                                      cATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCg                                                                                                    <  1:1402177/140‑1 (MQ=255)
                                      cATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCg                                                                                                    <  2:617605/140‑1 (MQ=255)
                                      cATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCg                                                                                                    <  1:1199243/140‑1 (MQ=255)
                                      cATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCg                                                                                                    <  1:813877/140‑1 (MQ=255)
                                      cATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATCCCAGCg                                                                                                    <  1:900122/140‑1 (MQ=255)
                                      cATTGTGCATCTGGTGTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCg                                                                                                    <  1:1366289/140‑1 (MQ=255)
                                        ttGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGAt                                                                                                  >  1:1478532/1‑140 (MQ=255)
                                            gCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCg                                                                                              >  1:64448/1‑140 (MQ=255)
                                             cATCTGGTTTCGAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCgt                                                                                             <  1:261783/140‑2 (MQ=255)
                                             cATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGa                                                                                             <  1:1146660/140‑1 (MQ=255)
                                             cATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGa                                                                                             <  1:261777/140‑1 (MQ=255)
                                             cATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGa                                                                                             <  1:261978/140‑1 (MQ=255)
                                                   gTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCg                                                                                       >  2:388960/1‑140 (MQ=255)
                                                    tttCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGt                                                                                      >  2:290952/1‑140 (MQ=255)
                                                         aGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCTCGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCa                                                                                 <  2:1872076/140‑1 (MQ=255)
                                                          gcgcCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCTGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAg                                                                                >  1:1272648/1‑140 (MQ=255)
                                                          gcgcCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCTGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAg                                                                                >  1:1276874/1‑140 (MQ=255)
                                                            gcCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAgcg                                                                              <  1:118112/140‑1 (MQ=255)
                                                            gcCGTTGCGGGTCTGCCGCCGGGCACCGTCCCGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTAAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAgcg                                                                              <  1:941036/140‑1 (MQ=255)
                                                                      gTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGaa                                                                    >  2:1797/1‑140 (MQ=255)
                                                                       tCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCTGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAg                                                                   <  2:1276874/140‑1 (MQ=255)
                                                                       tCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCTGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAg                                                                   <  2:1272648/140‑1 (MQ=255)
                                                                              ccgGGAAACGTCACGCTGGTGGATCAGGGCGGTCATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTTAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCt                                                            <  1:489087/140‑1 (MQ=255)
                                                                              acgGGAAACGTCTAGCTGGTGTATCAGGGCGGACATCTGTTTACCCAGTCGAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGTAATATGCCAGCGATGTCGAAGGACGTATTCTGCGGCGTATTGAAGCGATCCt                                                            <  1:1917100/139‑1 (MQ=255)
                                                                                      agTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTa                                                    <  2:1880521/139‑1 (MQ=255)
                                                                                        tCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTAtt                                                  <  1:1038574/140‑1 (MQ=255)
                                                                                        tCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTAtt                                                  <  1:1402479/140‑1 (MQ=255)
                                                                                        tCACGCTGGTGGATCAGGGCGGACATCTGTAAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTAtt                                                  <  1:1402144/140‑1 (MQ=255)
                                                                                              tggtggATCAGGGCGGTCATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGATGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGt                                            <  1:1230737/140‑1 (MQ=255)
                                                                                                gtggATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTaa                                          <  1:913639/140‑1 (MQ=255)
                                                                                                      cAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTaa                                    >  2:1414568/1‑140 (MQ=255)
                                                                                                      cAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTaa                                    >  1:750425/1‑140 (MQ=255)
                                                                                                        gggCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAata                                  >  2:1050155/1‑140 (MQ=255)
                                                                                                        gggCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAata                                  >  2:440158/1‑140 (MQ=255)
                                                                                                        gggCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAata                                  >  2:368908/1‑140 (MQ=255)
                                                                                                         ggCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAatat                                 <  1:1797/140‑1 (MQ=255)
                                                                                                          gCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATAtt                                >  1:1687743/1‑140 (MQ=255)
                                                                                                             gACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCAc                             >  1:273149/1‑140 (MQ=255)
                                                                                                                aTCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGcc                          <  1:832193/140‑1 (MQ=255)
                                                                                                                   tGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAg                       >  1:1072997/1‑140 (MQ=255)
                                                                                                                          ccAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACgg                >  1:982372/1‑140 (MQ=255)
                                                                                                                            aGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcg              >  2:1719960/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCATCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  1:1060813/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  2:1717958/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  1:970445/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  1:970440/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  1:1061897/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  2:916772/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  2:961188/1‑140 (MQ=255)
                                                                                                                             gTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGGTGGTAACGGTAATATTCACGCCCAGGTTACGgcgc             >  2:1519220/1‑140 (MQ=255)
                                                                                                                              tCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCa            >  2:1677666/1‑140 (MQ=255)
                                                                                                                                      cAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACt    >  1:918876/1‑140 (MQ=255)
                                                                                                                                        gCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTc  >  2:1693445/1‑140 (MQ=255)
                                                                                                                                        gCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTc  >  2:1603989/1‑140 (MQ=255)
                                                                                                                                        gCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTc  >  1:1294169/1‑140 (MQ=255)
                                                                                                                                        gCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTc  >  1:1294075/1‑140 (MQ=255)
                                                                                                                                        gCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCAAGGTTACGGCGCAGCTGGACTTc  >  1:1282119/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TTACCCGGCCGCGCACTCGATGAAGGGCAAATTAGCGCCATTGTGCATCTGGTTTCCAGCGCCGTTGCTGGTCTGCCGCCGGGAAACGTCACGCTGGTGGATCAGGGCGGACATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGCCGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTC  >  NC_000913/2013760‑2014035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: