Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,046,785 G→T 5.7% V616V (GTG→GTT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,046,7850GT 5.7% 250.8 / 2.6 70V616V (GTG→GTT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (41/25);  new base T (2/2);  total (43/27)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

CTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAAT  >  NC_000913/2046650‑2046923
                                                                                                                                       |                                                                                                                                          
ctcGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTTACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATg                                                                                                                                        <  1:408437/140‑1 (MQ=255)
ctcGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATg                                                                                                                                        <  2:1336169/140‑1 (MQ=255)
ctcGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATg                                                                                                                                        <  1:428754/140‑1 (MQ=255)
ctcGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATg                                                                                                                                        <  1:428760/140‑1 (MQ=255)
ctcGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATg                                                                                                                                        <  2:1645578/140‑1 (MQ=255)
ctcGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATg                                                                                                                                        <  1:1523914/140‑1 (MQ=255)
 tcGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGTCTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGc                                                                                                                                       >  1:1641492/1‑140 (MQ=255)
        gTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTaaa                                                                                                                                >  2:282653/1‑140 (MQ=255)
                 gtgtTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCccgcc                                                                                                                       >  2:285666/1‑140 (MQ=255)
                    ttCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCgtg                                                                                                                    >  1:1534727/1‑140 (MQ=255)
                       aGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCgtggtg                                                                                                                 <  2:352733/140‑1 (MQ=255)
                         gACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGaa                                                                                                               <  1:886790/140‑1 (MQ=255)
                          aCATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAat                                                                                                              <  1:1606559/140‑1 (MQ=255)
                            aTCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGACACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAatat                                                                                                            >  1:770432/1‑140 (MQ=255)
                              cACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCGCGCCGTGGTGAATATCa                                                                                                          >  2:841105/1‑140 (MQ=255)
                              cACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCa                                                                                                          >  1:68995/1‑140 (MQ=255)
                              cACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCa                                                                                                          >  2:841236/1‑140 (MQ=255)
                                   tttCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCt                                                                                                     >  2:1882004/1‑140 (MQ=255)
                                   tttCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCt                                                                                                     >  1:123222/1‑140 (MQ=255)
                                   tttCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCt                                                                                                     >  1:953165/1‑140 (MQ=255)
                                   tttCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCt                                                                                                     >  1:123217/1‑140 (MQ=255)
                                   tttCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCt                                                                                                     >  1:1612996/1‑140 (MQ=255)
                                   tttCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCt                                                                                                     >  1:827170/1‑140 (MQ=255)
                                     tCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGt                                                                                                   >  1:1304655/1‑140 (MQ=255)
                                     tCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGt                                                                                                   >  1:1728996/1‑140 (MQ=255)
                                        gACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTc                                                                                                >  1:1339207/1‑140 (MQ=255)
                                        gACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTc                                                                                                >  1:130877/1‑140 (MQ=255)
                                         aCTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCg                                                                                               >  2:1569580/1‑140 (MQ=255)
                                           tGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGAGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTc                                                                                             >  2:1857677/1‑140 (MQ=255)
                                                   tAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTCAAGGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGATTCGTCATCCCGaa                                                                                     >  2:1104931/1‑140 (MQ=255)
                                                           aCGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCg                                                                             <  2:1082851/140‑1 (MQ=255)
                                                           aCGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCg                                                                             <  2:1141205/140‑1 (MQ=255)
                                                           aCGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCg                                                                             <  2:1082813/140‑1 (MQ=255)
                                                            cGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGt                                                                            >  1:814484/1‑140 (MQ=255)
                                                            acaaaccTATACACATATCCTGACCACAGGATCGATTTATTGCAAGCTGAAGCTGATGACACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGt                                                                            >  1:272891/7‑140 (MQ=255)
                                                                aGCTATACCCAGATCCTGACCTCAGGAGCGATGTCTGGCACGCTGACGCTGATGCCTCAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAAt                                                                        <  1:392692/140‑1 (MQ=255)
                                                                     taCCCAGATGCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCTCAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGa                                                                   <  1:287178/140‑1 (MQ=255)
                                                                            aTCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATaa                                                            >  1:254362/1‑140 (MQ=255)
                                                                            aTCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATaa                                                            <  2:1396800/140‑1 (MQ=255)
                                                                            aTCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATaa                                                            >  1:1540213/1‑140 (MQ=255)
                                                                            aTCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATaa                                                            >  2:532884/1‑140 (MQ=255)
                                                                            aTCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATaa                                                            >  2:605738/1‑140 (MQ=255)
                                                                            aTCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAAGGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCTCTCGTCAATTAAGATTGATaa                                                            >  1:937886/1‑140 (MQ=255)
                                                                                  aCCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCg                                                      >  2:958390/1‑140 (MQ=255)
                                                                                  aCCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCg                                                      >  1:177962/1‑140 (MQ=255)
                                                                                         gAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTAtctc                                               >  1:1075399/1‑140 (MQ=255)
                                                                                            cGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTcc                                            >  1:432520/1‑140 (MQ=255)
                                                                                              aTGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTTGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCgg                                          >  1:1226282/1‑140 (MQ=255)
                                                                                              aTGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTTGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCgg                                          >  1:1226291/1‑140 (MQ=255)
                                                                                              aTGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCgg                                          <  2:1536682/140‑1 (MQ=255)
                                                                                                   tGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATaa                                                            <  2:235119/117‑1 (MQ=255)
                                                                                                   tGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATaa                                                            >  1:235119/1‑117 (MQ=255)
                                                                                                             acgctgaTGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGt                           <  1:1789815/140‑1 (MQ=255)
                                                                                                             acgctgaTGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGt                           <  1:1249508/140‑1 (MQ=255)
                                                                                                             acgctgaTGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGt                           <  1:1789767/140‑1 (MQ=255)
                                                                                                                  gaTGCCACAGCTGAATGGTGTTGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACgg                      <  2:1226291/140‑1 (MQ=255)
                                                                                                                  gaTGCCACAGCTGAATGGTGTTGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACgg                      <  2:1226282/140‑1 (MQ=255)
                                                                                                                  gaTGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACgg                      <  2:1764462/140‑1 (MQ=255)
                                                                                                                   aTGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGt                     >  1:1717899/1‑140 (MQ=255)
                                                                                                                     gCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAg                   >  1:353203/1‑140 (MQ=255)
                                                                                                                       cacaGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGATATCTCTCCGGCAATCCTATCGAGGTGACGgaagaa                 >  1:1287716/1‑140 (MQ=255)
                                                                                                                               aaTGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGt                     <  2:873159/128‑1 (MQ=255)
                                                                                                                               aaTGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGt                     >  1:873159/1‑128 (MQ=255)
                                                                                                                               aaTGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTgagaga         >  2:1533577/1‑140 (MQ=255)
                                                                                                                               aaTGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGAAGAACTgagaga         >  1:1004791/1‑140 (MQ=255)
                                                                                                                                 tGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGTGATg       >  2:1363188/1‑140 (MQ=255)
                                                                                                                                   gtgtGGATGCGGCTAAAGCCCCCGCCGTGGTGACTATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGaa     <  2:1872741/140‑1 (MQ=255)
                                                                                                                                   gtgtGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGaa     <  2:488068/140‑1 (MQ=255)
                                                                                                                                   gtgtGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGaa     <  1:285666/140‑1 (MQ=255)
                                                                                                                                   gtgtGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGaa     >  1:110277/1‑140 (MQ=255)
                                                                                                                                    tgtgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGaaa    >  1:1450216/1‑140 (MQ=255)
                                                                                                                                    tgtgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGaaa    >  2:780274/1‑140 (MQ=255)
                                                                                                                                    tgtgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGaaa    >  1:85438/1‑140 (MQ=255)
                                                                                                                                      tgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAAt  >  1:144423/1‑140 (MQ=255)
                                                                                                                                      tgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAAt  >  1:1568065/1‑140 (MQ=255)
                                                                                                                                      tgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAAt  >  1:1111811/1‑140 (MQ=255)
                                                                                                                                      tgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAAt  >  1:621187/1‑140 (MQ=255)
                                                                                                                                      tgGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTACGGCAATCCTATCGAGGTGACGGTAGAAGTGAGAGATGAAAAt  >  1:143446/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAATGGTGTGGATGCGGCTAAAGCCCCCGCCGTGGTGAATATCATTTCTGTTTCGTCATCCCGAACTCACTCGTCAATTAAGATTGATAAGGACCGTTATCTCTCCGGCAATCCTATCGAGGTGACGGTAGAACTGAGAGATGAAAAT  >  NC_000913/2046650‑2046923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑