| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,403 | G→T | 6.3% | I51I (ATC→ATA) | wcaB ← | putative acyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,403 | 0 | G | T | 6.3% | 233.4 / 2.8 | 63 | I51I (ATC→ATA) | wcaB | putative acyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (23/36); new base T (2/2); total (25/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
AGTGACGCCGTGGCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATGCAGCACGGGCGTAAACTCCAGCTGTTGGCGC > NC_000913/2132265‑2132539 | aGTGACGCCGTGGCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGa > 1:1088915/1‑140 (MQ=255)aGTGACGCCGTGGCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGa > 1:1579646/1‑140 (MQ=255) aCGCCGTGGCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAAt > 1:1138251/1‑140 (MQ=255) aCGCCGTGGCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAAt > 1:754815/1‑140 (MQ=255) cGCCGTGGCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATg < 1:340620/140‑1 (MQ=255) cGCCGTGGCGAATGGTAAAATCATCCCCCGCCACCCCGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAACAGCATTCGGTGATAATg < 1:340619/140‑1 (MQ=255) ggCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATAc < 2:674048/140‑1 (MQ=255) tGGTAAAATCATCCCCCGCTACCTCGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCAcc < 1:1738634/140‑1 (MQ=255) tGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCAcc < 1:819882/140‑1 (MQ=255) tGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCAcc < 1:1370014/140‑1 (MQ=255) tGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCAcc < 1:1487544/140‑1 (MQ=255) tGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCAcc < 1:1896577/140‑1 (MQ=255) tAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACcagc > 2:1651729/1‑140 (MQ=255) tAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACcagc > 2:1651675/1‑140 (MQ=255) aaaTCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCTTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACcagcag > 1:494025/1‑140 (MQ=255) aaaTCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACcagcag > 2:1167776/1‑140 (MQ=255) aaaTCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGAGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATATCAAAACCGAAAAAGCATACGGTGATAATGCGATACAGCACcagcag > 1:494013/1‑140 (MQ=255) tcCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGgc < 1:1773771/140‑1 (MQ=255) tcCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGgc > 1:1617696/1‑140 (MQ=255) tcCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGgc > 1:155953/1‑140 (MQ=255) tcCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGgc > 2:740688/1‑140 (MQ=255) tACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAACGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCcaca < 1:1197156/140‑1 (MQ=255) cgacgGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCttt < 2:764008/140‑1 (MQ=255) tAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGGAAAAGCAATCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCca < 2:928842/140‑1 (MQ=255) aCCGTGTTGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCcaca < 1:155809/140‑1 (MQ=255) aCCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCcaca < 1:722703/140‑1 (MQ=255) tGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGa > 1:1329581/1‑140 (MQ=255) aTGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAAc > 1:763577/1‑140 (MQ=255) tAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaaaa < 2:1721700/140‑1 (MQ=255) gcggcgGCCAATGGTCGCGGCAGCGTGGATTTCATAACCGAAATAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCaa < 2:1590461/140‑1 (MQ=255) gcggcgGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCaa < 2:318094/140‑1 (MQ=255) gcggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTTATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaaa < 2:1067436/129‑1 (MQ=255) gcggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTTATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaaa < 2:1067379/129‑1 (MQ=255) gcggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTTATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaaa > 1:1067436/1‑129 (MQ=255) gcggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTTATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaaaa > 1:1067379/1‑129 (MQ=255) ggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGa < 1:537719/140‑1 (MQ=255) ggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGa < 1:507387/140‑1 (MQ=255) ggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGa < 1:1304206/140‑1 (MQ=255) ggcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGa < 2:1287506/140‑1 (MQ=255) gcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGAt > 1:1761751/1‑140 (MQ=255) gcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGAt > 1:1761788/1‑140 (MQ=255) gcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGAt > 1:347090/1‑140 (MQ=255) gcCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGAt > 1:649827/1‑140 (MQ=255) aTGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGc < 1:428317/140‑1 (MQ=255) ggTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCaa < 1:1635106/140‑1 (MQ=255) gTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAg < 1:1770028/140‑1 (MQ=255) cgcgGCAGCCTGTATTTCAGAACCGAAACAGCATTCGGTGATAATGCGTTACAGCACCAGCAGCGGGGCCGACCACAGATTGTTGAGGACGTTCTTTTTGAGCCACACCGAACAAAAATGAGCAACACGCTAGGCAAGaa < 2:156762/140‑1 (MQ=255) cgcgGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGaa < 2:1572552/140‑1 (MQ=255) cgcgGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGaa < 2:1560520/140‑1 (MQ=255) cgGCAGCCTGGATTTCATAACCGATAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaa < 1:356318/113‑1 (MQ=255) cgGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACaa > 2:356318/1‑113 (MQ=255) ggCAGCCTGGATTTCCGAACCGAAAAAGCATTCGGTGATAATGCGATAGAGCACCAGCAGCGGGGCCGCCCCCAGATTGTTGAGGACGTTCTTGTGGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCa < 2:1215186/140‑1 (MQ=255) ggCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCa < 1:199078/140‑1 (MQ=255) tGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATgcagca < 2:124142/140‑1 (MQ=255) tGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATgcagca < 1:384668/140‑1 (MQ=255) tGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATgcagca < 1:1275925/140‑1 (MQ=255) aTAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATGCAGCACGGGCGTa > 2:1766161/1‑140 (MQ=255) aTAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATGCAGCACGGGCGTa > 2:1766235/1‑140 (MQ=255) aTAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATGCAGCACGGGCGTa > 2:210000/1‑140 (MQ=255) tAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGaa > 2:574058/1‑121 (MQ=255) tAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGaa < 1:574058/121‑1 (MQ=255) aTTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATGCAGCACGGGCGTAAACTCCAGCTGTTg < 1:365339/140‑1 (MQ=255) ggTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATGCAGCACGGGCGTAAACTCCAGCTGTTGgcgc < 1:973852/140‑1 (MQ=255) | AGTGACGCCGTGGCGAATGGTAAAATCATCCCCCGCTACCACGTTTTTATTGATCACGACGGCGTAACCGTGATGGATAGTAAAGCGGCGGCCAATGGTCGCGGCAGCCTGGATTTCATAACCGAAAAAGCATTCGGTGATAATGCGATACAGCACCAGCAGCGGGGCCGCCCACAGATTGTTGAGGACGTTCTTTTTGCGCCACACCGAACAAAAATGAGCAACACGATAGGCAAGAACCATGCAGCACGGGCGTAAACTCCAGCTGTTGGCGC > NC_000913/2132265‑2132539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |