Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,257,293 C→G 5.0% A15P (GCA→CCA)  psuT ← putative nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,257,2930CG 5.0% 264.5 / 2.9 72A15P (GCA→CCA) psuTputative nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base C (40/27);  new base G (2/2);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTA  >  NC_000913/2257161‑2257431
                                                                                                                                    |                                                                                                                                          
ccTGTTCTACTGCCCATTTTCCCGGTGGGACGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGGGCGGCTCCACCCGGGCGCAAACTGATGCTCTTTTTAGTCACTGACAACAGAAATGCTATTGCCAGTAac                                                                                                                                     >  1:908759/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAATTAGAGCATGATGACACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGGCAGTAac                                                                                                                                     >  1:1846197/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCACACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTCac                                                                                                                                     >  1:1094672/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGGCAGTAac                                                                                                                                     >  1:1846295/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAac                                                                                                                                     >  2:1583946/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAac                                                                                                                                     >  1:754833/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAac                                                                                                                                     >  1:1097018/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAac                                                                                                                                     >  1:1096438/1‑140 (MQ=255)
ccTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAac                                                                                                                                     >  1:1096420/1‑140 (MQ=255)
ccTCTTCAACTGCCCATTTTCCCGCTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGAGCGCAAACTGATGCTCTTTTTATACACAGACAACAGAAATTCAATTTCCAGTAac                                                                                                                                     >  2:620643/1‑140 (MQ=255)
 cTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAaca                                                                                                                                    >  1:1489930/1‑140 (MQ=255)
      cTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACaccacc                                                                                                                               <  1:276022/140‑1 (MQ=255)
       tACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACAccacca                                                                                                                              <  1:1419189/140‑1 (MQ=255)
       tACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTATTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACAccacca                                                                                                                              <  1:1918414/140‑1 (MQ=255)
       tACTGCCCAATTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACAccacca                                                                                                                              <  1:1419463/140‑1 (MQ=255)
            cccATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATcccc                                                                                                                         >  2:1260191/1‑140 (MQ=255)
            cccATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATcccc                                                                                                                         >  1:1216297/1‑140 (MQ=255)
              cATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCcac                                                                                                                       <  2:1502649/140‑1 (MQ=255)
              cATTGTCCCGGTGGGTAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCATCCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCcac                                                                                                                       <  2:47239/140‑1 (MQ=255)
                     ccGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACtt                                                                                                                <  2:1256655/140‑1 (MQ=255)
                     ccGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACtt                                                                                                                <  1:1041898/140‑1 (MQ=255)
                     ccGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACtt                                                                                                                <  1:1119582/140‑1 (MQ=255)
                             aaGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATtat                                                                                                        <  1:922511/140‑1 (MQ=255)
                              aGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATtata                                                                                                       <  1:1777178/140‑1 (MQ=255)
                              aGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATtata                                                                                                       <  2:236628/140‑1 (MQ=255)
                                  gagCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACAGACAACAGAAATGCTATTGCCAGTATCACCACCATCCCCACAACACTTCTCATTATATCCa                                                                                                   <  2:122917/140‑1 (MQ=255)
                                     cATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAAcacc                                                                                                                                  <  1:1047868/106‑1 (MQ=255)
                                     cATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAAcacc                                                                                                                                  >  2:1051370/1‑106 (MQ=255)
                                     cATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAAcacc                                                                                                                                  >  2:1047868/1‑106 (MQ=255)
                                     cATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAAcacc                                                                                                                                  <  1:1051370/106‑1 (MQ=255)
                                       tgatgCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATg                                                                                              >  1:1312469/1‑140 (MQ=255)
                                       tgatgCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATg                                                                                              >  2:1527274/1‑140 (MQ=255)
                                          tgCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGAtt                                                                                           >  1:558939/1‑140 (MQ=255)
                                          tgCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGAtt                                                                                           >  1:652922/1‑140 (MQ=255)
                                          tgCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGAtt                                                                                           >  1:1491446/1‑140 (MQ=255)
                                               ccaATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATCTCCATAATGATTTTccc                                                                                      >  2:1731596/1‑140 (MQ=255)
                                                         tgtGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTAGTGCCAGTACCACCACCCTCCCCACAACACTTCTCAGGATATCCCTAATGATTTTCCCTTCCTGCCgg                                                                            <  2:290542/138‑1 (MQ=255)
                                                          ttGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGt                                                                           >  2:1614654/1‑140 (MQ=255)
                                                          ttGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGt                                                                           >  1:216919/1‑140 (MQ=255)
                                                          ttGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGt                                                                           >  2:1255133/1‑140 (MQ=255)
                                                          ttGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGt                                                                           >  2:74974/1‑140 (MQ=255)
                                                          ttGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGt                                                                           >  2:1255066/1‑140 (MQ=255)
                                                                     gTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGt                                                                <  1:1081517/140‑1 (MQ=255)
                                                                     gTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGt                                                                <  1:1081661/140‑1 (MQ=255)
                                                                        cGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAg                                                             >  2:412764/1‑140 (MQ=255)
                                                                        cGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAg                                                             >  2:413029/1‑140 (MQ=255)
                                                                                aCCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGt                                                     >  1:1044922/1‑140 (MQ=255)
                                                                                aCCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGt                                                     >  1:1249433/1‑140 (MQ=255)
                                                                                    tGCGCAATCTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCCGTAACACCACCATCCCCCCAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTg                                                 <  2:647283/140‑1 (MQ=255)
                                                                                    tGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTg                                                 >  2:1470638/1‑140 (MQ=255)
                                                                                    tGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTg                                                 >  2:679563/1‑140 (MQ=255)
                                                                                    tGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTg                                                 >  1:1425005/1‑140 (MQ=255)
                                                                                     gcgcAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTgg                                                >  1:1442944/1‑140 (MQ=255)
                                                                                      cgcAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGGCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGt                                               <  2:1846197/140‑1 (MQ=255)
                                                                                      cgcAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGGCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGt                                               <  2:1846295/140‑1 (MQ=255)
                                                                                      cgcAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGt                                               >  2:1743084/1‑140 (MQ=255)
                                                                                          aaCTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGc                                           <  2:1489930/140‑1 (MQ=255)
                                                                                          aaCTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGACAGTAACACCACCATCCCCCAATCACATCTCATTTTATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGc                                           <  2:1275508/140‑1 (MQ=255)
                                                                                             tGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTtg                                        <  1:168768/140‑1 (MQ=255)
                                                                                             tGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTtg                                        <  1:970294/140‑1 (MQ=255)
                                                                                             tGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCACATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTtg                                        <  1:1024215/140‑1 (MQ=255)
                                                                                               aTGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTtgat                                      <  2:1752920/140‑1 (MQ=255)
                                                                                               aTGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTtgat                                      <  2:1896212/140‑1 (MQ=255)
                                                                                               aTGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTtgat                                      <  2:501999/140‑1 (MQ=255)
                                                                                                               cTGACAACAGAAATTCTATTGCCAGTAACACCACCAACCCCACAACACATATCATTATATCCAAAATGATTTTCCCGTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTTGTATGCTTGATGAGTACGGGCGACGGc                      >  1:371320/1‑140 (MQ=255)
                                                                                                               cTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGc                      >  1:413152/1‑140 (MQ=255)
                                                                                                                     acaGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTg                <  1:1260191/140‑1 (MQ=255)
                                                                                                                                 ttGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAAt    >  1:1417465/1‑140 (MQ=255)
                                                                                                                                 ttGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAAt    >  1:1221632/1‑140 (MQ=255)
                                                                                                                                 ttGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAAt    >  2:223845/1‑140 (MQ=255)
                                                                                                                                 ttGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAAt    >  2:222956/1‑140 (MQ=255)
                                                                                                                                   gCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTa  >  1:1071650/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                          
CCTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTA  >  NC_000913/2257161‑2257431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: