Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,259,286	T→C	5.1%	K4R (AAG→AGG)	psuK ←	pseudouridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,286	0	T	C	5.1%	257.5 / 4.1	79	K4R (AAG→AGG)	psuK	pseudouridine kinase
Reads supporting (aligned to +/- strand):  ref base T (31/44);  new base C (2/2);  total (33/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

TGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAAT  >  NC_000913/2259154‑2259425                                                                                                                                     |                                                                                                                                            tgtgCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTcgcg                                                                                                                                      <  1:315434/140‑1 (MQ=255)   tgCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCa                                                                                                                                    >  1:1647090/1‑140 (MQ=255)       atatTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGc                                                                                                                                >  1:1873146/1‑140 (MQ=255)             cgcgCTACTCCACCAGGGGTAAATTTTATTTTACCTTGATTTGATTCCGCATATTTTAATGATTCATGTGAATATGCGTCGTAATAATTATTCGCAGTAGCTTTAATTTCGAGTTAATCGTTTTCGCTCATTGCTCGTcg                                                                                                                          >  2:197083/1‑139 (MQ=255)             cgcCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCTCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTAAAATTACGACATAATCCTTTTCGCGCATAGCACGTcc                                                                                                                          >  1:1169515/1‑140 (MQ=255)                  tACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCCTTTCGCGCATAGCACGTCCTCTgg                                                                                                                     >  1:494885/1‑140 (MQ=255)                  tACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCCTTTCGCGCATAGCACGTCCTCTgg                                                                                                                     >  1:494835/1‑140 (MQ=255)                         ccaGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTaaaaaa                                                                                                              >  1:1756354/1‑140 (MQ=255)                          caGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAAt                                                                                                             >  1:1900446/1‑140 (MQ=255)                            ggCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTa                                                                                                           >  1:429484/1‑140 (MQ=255)                            ggCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTa                                                                                                           >  2:267590/1‑140 (MQ=255)                            ggCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTa                                                                                                           >  1:860043/1‑140 (MQ=255)                                 aaaTTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAAc                                                                                                      >  2:741016/1‑140 (MQ=255)                                           ttaCCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATc                                                                                            <  2:584478/140‑1 (MQ=255)                                              ccTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCcac                                                                                         <  1:1365082/140‑1 (MQ=255)                                              ccTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCcac                                                                                         >  1:121366/1‑140 (MQ=255)                                              ccTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCcac                                                                                         <  1:400780/140‑1 (MQ=255)                                              ccTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCcac                                                                                         >  1:120848/1‑140 (MQ=255)                                               cTGGATTTGAATCCGCATAATTTAATGATTCATGTGTATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCcaca                                                                                        <  2:1070282/140‑1 (MQ=255)                                                 ggATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAg                                                                                      <  1:120067/140‑1 (MQ=255)                                                   aTTTGAATCCGCATAATTTAATGATTCATGTGAATATCCCGCGACATCAATATTCGCCGAACCTATAATTACGACATAAACCTATACGCGCATAGCACCTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGaa                                                                                    >  2:255993/1‑140 (MQ=255)                                                      tGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAatat                                                                                 <  1:539228/140‑1 (MQ=255)                                                         aTCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAGAATTACCAACCCTGTCAATCCACAAGAATATAGa                                                                              <  1:1331277/140‑1 (MQ=255)                                                         aTCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGa                                                                              <  2:75373/140‑1 (MQ=255)                                                         aTCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGa                                                                              <  1:1664374/140‑1 (MQ=255)                                                          tCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGAt                                                                             <  1:1660833/140‑1 (MQ=255)                                                            cGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATAc                                                                           <  1:1061396/140‑1 (MQ=255)                                                            cGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATAc                                                                           >  1:1278662/1‑140 (MQ=255)                                                              cATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTg                                                                         <  2:1162022/140‑1 (MQ=255)                                                                   tttAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAg                                                                    <  2:1354662/140‑1 (MQ=255)                                                                    ttAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGa                                                                   <  1:1170175/140‑1 (MQ=255)                                                                    ttAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGa                                                                   <  1:579415/140‑1 (MQ=255)                                                                      aaTGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACg                                                                 <  2:877142/140‑1 (MQ=255)                                                                             cATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTaaa                                                          <  2:1625324/140‑1 (MQ=255)                                                                             cATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTaaa                                                          >  2:540147/1‑140 (MQ=255)                                                                             cATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTaaa                                                          >  2:558781/1‑140 (MQ=255)                                                                             cATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTaaa                                                          >  1:1616890/1‑140 (MQ=255)                                                                             cATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTaaa                                                          <  2:1624713/140‑1 (MQ=255)                                                                             cATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTaaa                                                          >  1:1396698/1‑140 (MQ=255)                                                                             cATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTaaa                                                          >  2:1277187/1‑140 (MQ=255)                                                                              aTGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAAc                                                         <  1:990832/140‑1 (MQ=255)                                                                              aTGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAAc                                                         >  2:990832/1‑140 (MQ=255)                                                                              aTGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAAc                                                         <  1:1740907/140‑1 (MQ=255)                                                                                gtgAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACAt                                                       <  1:1848419/140‑1 (MQ=255)                                                                                gtgAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACAt                                                       <  2:321488/140‑1 (MQ=255)                                                                                gtgAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACAt                                                       <  2:321477/140‑1 (MQ=255)                                                                                     tatCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAATAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGtt                                                  >  2:1092128/1‑140 (MQ=255)                                                                                     tatCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGtt                                                  >  2:1092151/1‑140 (MQ=255)                                                                                     tatCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGtt                                                  >  2:1327316/1‑140 (MQ=255)                                                                                     tatCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGtt                                                  >  2:1774545/1‑140 (MQ=255)                                                                                     tatCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGtt                                                  <  1:737957/140‑1 (MQ=255)                                                                                      atCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTc                                                 <  2:753280/140‑1 (MQ=255)                                                                                             gACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTa                                          <  1:583386/140‑1 (MQ=255)                                                                                             gACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTa                                          <  1:1829100/140‑1 (MQ=255)                                                                                             gACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTa                                          <  1:535891/140‑1 (MQ=255)                                                                                               cATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTaaa                                        >  1:577884/1‑140 (MQ=255)                                                                                               cATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTaaa                                        <  1:291267/140‑1 (MQ=255)                                                                                                 tCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACa                                      <  2:194076/140‑1 (MQ=255)                                                                                                 tCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACa                                      <  2:194226/140‑1 (MQ=255)                                                                                                 tCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACa                                      <  2:194232/140‑1 (MQ=255)                                                                                                   aaTATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACAtg                                    <  1:1708392/140‑1 (MQ=255)                                                                                                   aaTATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACAtg                                    <  1:1446436/140‑1 (MQ=255)                                                                                                     tatTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACAtgtg                                  <  1:252696/140‑1 (MQ=255)                                                                                                       ttCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCt                                <  1:525374/140‑1 (MQ=255)                                                                                                       ttCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCt                                <  1:106704/140‑1 (MQ=255)                                                                                                         cGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCa                              <  2:554308/140‑1 (MQ=255)                                                                                                          gCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCAt                             <  1:1908875/140‑1 (MQ=255)                                                                                                                 cTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGc                      <  1:635383/140‑1 (MQ=255)                                                                                                                      aTTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCACAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGtt                 <  1:264835/140‑1 (MQ=255)                                                                                                                       ttACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGttt                >  1:572134/1‑140 (MQ=255)                                                                                                                       ttACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGttt                >  1:572215/1‑140 (MQ=255)                                                                                                                        tACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGtttt               >  1:684938/1‑140 (MQ=255)                                                                                                                         aCGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTa                                                            >  2:580854/1‑94 (MQ=255)                                                                                                                         aCGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTa                                                            <  1:580854/94‑1 (MQ=255)                                                                                                                           gACATAATCCTTTTCGCGCAAAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAAGCCACATGTATAGAGATACTGCGAAGACGGGTGGAACATGTGTTCATAGTTGAACTTGGGCTCGTAGTGTGCATGATTTACg            >  2:493058/1‑140 (MQ=255)                                                                                                                             cATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACgta          <  1:1167287/140‑1 (MQ=255)                                                                                                                             cATAATCCCTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACgta          <  2:494885/140‑1 (MQ=255)                                                                                                                             cATAATCCCTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACgta          <  2:494835/140‑1 (MQ=255)                                                                                                                                    cTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACgtaaagtaaa   >  1:4540/1‑140 (MQ=255)                                                                                                                                     ttttCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAAt  <  2:949218/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                            TGTGCAATATTGCGCCCTACTCCACCAGGCGTAAATTTTATTTTACCTGGATTTGAATCCGCATAATTTAATGATTCATGTGAATATCCGGCGACATCAATATTCGCCGAACCTATAATTACGACATAATCCTTTTCGCGCATAGCACGTCCTCTGGTAAAAAATTACCAACCCTGTCAATCCACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAAT  >  NC_000913/2259154‑2259425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑