Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,826,080 G→T 5.0% M83I (ATG→ATT srlA → glucitol/sorbitol‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,826,0800GT 5.0% 397.3 / 3.2 108M83I (ATG→ATTsrlAglucitol/sorbitol‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base G (68/34);  new base T (3/3);  total (71/37)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCC  >  NC_000913/2825941‑2826218
                                                                                                                                           |                                                                                                                                          
tgctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATg                                                                                                                                            >  1:271257/1‑140 (MQ=255)
  ctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTTAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATTAc                                                                                                                                          >  2:1194604/1‑140 (MQ=255)
  ctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTTAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATTAc                                                                                                                                          >  2:1197336/1‑140 (MQ=255)
  ctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTTAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATTAc                                                                                                                                          >  2:1197465/1‑140 (MQ=255)
  ctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGAc                                                                                                                                          >  1:1015591/1‑140 (MQ=255)
  ctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGAc                                                                                                                                          >  1:379958/1‑140 (MQ=255)
  ctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGAc                                                                                                                                          >  1:1342866/1‑140 (MQ=255)
        aTCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAg                                                                                                                                    >  1:640954/1‑140 (MQ=255)
              aaCGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTggg                                                                                                                              <  1:1785259/140‑1 (MQ=255)
               aCGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGAGCCTAAGCCTGGGt                                                                                                                             >  2:1866781/1‑140 (MQ=255)
               aCGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGt                                                                                                                             >  1:935843/1‑140 (MQ=255)
               aCGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGt                                                                                                                             >  1:1148074/1‑140 (MQ=255)
               aCGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGt                                                                                                                             >  1:1703190/1‑140 (MQ=255)
                cGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGTCCCTAAGCCTGGGTc                                                                                                                            >  1:943128/1‑140 (MQ=255)
                   aCTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCt                                                                                                                         >  1:364568/1‑140 (MQ=255)
                       aTTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTAt                                                                                                                     >  2:801881/1‑140 (MQ=255)
                       aTTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTAt                                                                                                                     >  2:827239/1‑140 (MQ=255)
                       aTTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTAt                                                                                                                     >  1:498552/1‑140 (MQ=255)
                       aTTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGCGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTAt                                                                                                                     >  1:499211/1‑140 (MQ=255)
                             tttATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGa                                                                                                               <  1:1649106/140‑1 (MQ=255)
                              ttATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGaa                                                                                                              <  2:1080810/140‑1 (MQ=255)
                               tATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGaaa                                                                                                             <  1:550988/140‑1 (MQ=255)
                               tATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGaaa                                                                                                             <  1:605205/140‑1 (MQ=255)
                                 tCGGTCAGCATCGTATTGAACGTTTTGCTCAAGGTTGCGCCGGTAAGCCTGTTTACCGTTAGGTAGTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACACTAAGCCTGGGTCGCTTTATGCCGGTAAAg                                                                                                           >  2:326562/1‑140 (MQ=255)
                                 tCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAg                                                                                                           >  2:273462/1‑140 (MQ=255)
                                 tCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAg                                                                                                           >  2:1879055/1‑140 (MQ=255)
                                 tCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAg                                                                                                           >  2:1878732/1‑140 (MQ=255)
                                 tCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGAAGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAg                                                                                                           >  2:135971/1‑140 (MQ=255)
                                       aGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCTCGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACaaa                                                                                                     <  2:581121/140‑1 (MQ=255)
                                         catcatAATTAACGTTATGCTCAACGTTGAGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGAGCATTTGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGACAGGGTCTCTTTATGACGTACAAGAACAAAcc                                                                                                   >  2:673796/1‑140 (MQ=255)
                                              tATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGct                                                                                              >  1:1806459/1‑140 (MQ=255)
                                               aTTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGcta                                                                                             <  2:1585428/140‑1 (MQ=255)
                                                   aaCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCtactac                                                                                         >  1:982026/1‑140 (MQ=255)
                                                   aaCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCtactac                                                                                         >  1:1074424/1‑140 (MQ=255)
                                                   aaCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCtactac                                                                                         >  1:1379750/1‑140 (MQ=255)
                                                   aaCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCtactac                                                                                         >  2:893725/1‑140 (MQ=255)
                                                   aaCGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCtactac                                                                                         >  1:1178393/1‑140 (MQ=255)
                                                     cGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTAcgc                                                                                       >  2:447864/1‑140 (MQ=255)
                                                     cGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTAcgc                                                                                       >  2:1195183/1‑140 (MQ=255)
                                                     cGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTAcgc                                                                                       >  1:1764279/1‑140 (MQ=255)
                                                        tttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGTAAAGTACAAACCCAGCTACTACgcggc                                                                                    >  1:1745984/1‑140 (MQ=255)
                                                        tttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggc                                                                                    >  1:548908/1‑140 (MQ=255)
                                                        tttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggc                                                                                    >  2:934506/1‑140 (MQ=255)
                                                         ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                                                                                   <  2:310521/140‑1 (MQ=255)
                                                         gtGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                                                                                   <  1:738714/139‑1 (MQ=255)
                                                           gCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCggcggc                                                                                 <  1:1372928/140‑1 (MQ=255)
                                                                       gcCGGTAACCCTGTTTACCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTg                                                                     <  2:1278610/140‑1 (MQ=255)
                                                                           gTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACACAGCTACTACGCGGCGGCCTCTTATAGCTGCCAc                                                                 >  2:1072235/1‑140 (MQ=255)
                                                                             aaCCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTc                                                               <  1:1707918/140‑1 (MQ=255)
                                                                              aCCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCa                                                              >  1:1720122/1‑140 (MQ=255)
                                                                                   gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGc                                                                                                                                   <  1:779925/66‑1 (MQ=255)
                                                                                   gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGc                                                                                                                                   >  2:779925/1‑66 (MQ=255)
                                                                                   gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCaatgaa                                                         >  1:328084/1‑140 (MQ=255)
                                                                                   gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCaatgaa                                                         >  1:788429/1‑140 (MQ=255)
                                                                                     ttCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCaatgaatg                                                       >  1:153700/1‑140 (MQ=255)
                                                                                     ttCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCAACTACGCGGCGGCCTCTTATAGCTGCCACTCaatgaatg                                                       >  1:401469/1‑140 (MQ=255)
                                                                                          gTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                                  >  2:1430543/1‑140 (MQ=255)
                                                                                          gTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                                  >  2:532918/1‑140 (MQ=255)
                                                                                          gTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                                  >  1:102802/1‑140 (MQ=255)
                                                                                          gTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                                  >  2:792202/1‑140 (MQ=255)
                                                                                          gTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                                  >  2:1430354/1‑140 (MQ=255)
                                                                                          gTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                                  >  1:1192276/1‑140 (MQ=255)
                                                                                          gTTACCTACAGTTACCGTGCATTGGCACGTTTGTCATTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                                  >  1:1192313/1‑140 (MQ=255)
                                                                                                tACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcccc                                            <  2:908612/140‑1 (MQ=255)
                                                                                                tACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcccc                                            <  2:381936/140‑1 (MQ=255)
                                                                                                tACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcccc                                            <  2:381805/140‑1 (MQ=255)
                                                                                                tACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcccc                                            <  2:1586315/140‑1 (MQ=255)
                                                                                                     ttACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCatat                                       <  1:1705179/140‑1 (MQ=255)
                                                                                                     ttACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCatat                                       >  1:1491260/1‑140 (MQ=255)
                                                                                                     ttACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCatat                                       <  1:515637/140‑1 (MQ=255)
                                                                                                      tACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATTACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATc                                      <  1:1197336/140‑1 (MQ=255)
                                                                                                      tACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATTACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATc                                      <  1:1197465/140‑1 (MQ=255)
                                                                                                      tACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATTACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATc                                      <  1:1194604/140‑1 (MQ=255)
                                                                                                       aCCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCa                                     >  1:578515/1‑140 (MQ=255)
                                                                                                       aCCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCa                                     >  1:1641672/1‑140 (MQ=255)
                                                                                                       aCCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCa                                     >  1:1504980/1‑140 (MQ=255)
                                                                                                       aCCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCa                                     >  1:1504694/1‑140 (MQ=255)
                                                                                                        ccGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCaa                                    >  1:762878/1‑140 (MQ=255)
                                                                                                        ccGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCaa                                    >  1:1783083/1‑140 (MQ=255)
                                                                                                          gTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTccc                                             >  2:734039/1‑129 (MQ=255)
                                                                                                          gTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTccc                                             <  1:734039/129‑1 (MQ=255)
                                                                                                            gCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCt                                >  1:1771673/1‑140 (MQ=255)
                                                                                                                  gCACGTTTGTCTTTTGCAATCCGTTGACACAAAGCCTGCGTCGCTTTAAGCCGGTAAAGTAAAAACCCAGCTACTACGCGGCGGCCTCGAATAGCTGCCACTCAATGAAAGGCGTGTTCCCCCATATCCACCCTGGCGaa                          >  2:1732493/1‑140 (MQ=255)
                                                                                                                  gCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGaa                          <  1:1677954/140‑1 (MQ=255)
                                                                                                                  gCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGaa                          >  1:1285264/1‑140 (MQ=255)
                                                                                                                  gCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGaa                          <  1:579764/140‑1 (MQ=255)
                                                                                                                  gCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGaa                          <  1:1300115/140‑1 (MQ=255)
                                                                                                                    aCGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACt                        >  1:1358603/1‑140 (MQ=255)
                                                                                                                     cGTTTGTCTTTTGCAATCCGATGTCCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCATTGTATGGCCTCTTCCCCCATATCAACCCTGGCGAACtg                       >  1:424663/1‑140 (MQ=255)
                                                                                                                          gTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgt                  <  2:1192313/140‑1 (MQ=255)
                                                                                                                          gTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgt                  <  1:1518147/140‑1 (MQ=255)
                                                                                                                          gTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgt                  <  2:1192276/140‑1 (MQ=255)
                                                                                                                           tCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgtt                 <  2:495471/140‑1 (MQ=255)
                                                                                                                           tCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGATTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgtt                 <  2:65030/140‑1 (MQ=255)
                                                                                                                                tGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCt            >  2:222825/1‑140 (MQ=255)
                                                                                                                                tGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTGTATCt            >  2:248886/1‑140 (MQ=255)
                                                                                                                                tGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGGTTATCt            >  1:8285/1‑140 (MQ=255)
                                                                                                                                tGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACGGTTTGTTTATCt            >  2:248885/1‑140 (MQ=255)
                                                                                                                                tGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCGTCTCGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGACCTGTTTGTTTATCt            >  2:248869/1‑140 (MQ=255)
                                                                                                                                          tGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGcc  <  1:613640/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCC  >  NC_000913/2825941‑2826218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: