Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,859,142 G→A 90.1% A684T (GCC→ACC)  mutS → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,859,1420GA90.1% 291.7 / 21.5 101A684T (GCC→ACC) mutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (4/6);  new base A (37/54);  total (41/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

Only 100 of 101 total aligned reads displayed.

GCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTA  >  NC_000913/2859005‑2859278
                                                                                                                                         |                                                                                                                                        
gCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACAcc                                                                                                                                        >  1:1845118/1‑140 (MQ=255)
       aCCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAAt                                                                                                                                 <  2:750245/140‑1 (MQ=255)
        ccGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATa                                                                                                                                <  2:768661/140‑1 (MQ=255)
        ccGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATa                                                                                                                                <  1:588065/140‑1 (MQ=255)
        acGGCACAAAATGTCGAGATTGGACCTATCGATCTCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAAAACCACCGAATa                                                                                                                                <  1:588162/139‑1 (MQ=255)
          ggCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACa                                                                                                                              <  1:1795457/140‑1 (MQ=255)
           gCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAg                                                                                                                             >  2:896123/1‑140 (MQ=255)
           gCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAg                                                                                                                             >  1:500450/1‑140 (MQ=255)
            cacaAAAAGTCGAGATTGGACCTATCGATCTCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGTGGTCTGAAACCGCCAATTTTTTACATAACACCACCGAATACAGt                                                                                                                            >  1:1497712/1‑140 (MQ=255)
            cacaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGt                                                                                                                            <  2:456512/140‑1 (MQ=255)
            cacaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGt                                                                                                                            <  2:882711/140‑1 (MQ=255)
            cacaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGt                                                                                                                            >  1:1561973/1‑140 (MQ=255)
            cacaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGt                                                                                                                            >  1:1561964/1‑140 (MQ=255)
            cacaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGt                                                                                                                            >  1:1561948/1‑140 (MQ=255)
             acaAAACGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTc                                                                                                                           <  2:738542/140‑1 (MQ=255)
             acaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTc                                                                                                                           <  2:97001/140‑1 (MQ=255)
                 aaaGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGgt                                                                                                                       <  1:539668/140‑1 (MQ=255)
                   aGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGgtgt                                                                                                                     <  2:896723/140‑1 (MQ=255)
                     tCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTa                                                                                                                   >  1:929527/1‑140 (MQ=255)
                          aTTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGa                                                                                                              <  1:38331/140‑1 (MQ=255)
                           ttGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGAt                                                                                                             <  2:739282/140‑1 (MQ=255)
                                ccTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGAt                                                                                                        <  2:1120579/140‑1 (MQ=255)
                                   atcgatcgCATCTTTACCCGCGTTGGCGCGGCAGATGACCTTGCGTCCGGGAGCTCAAGCATTTTGGTGGAGATGTCTGATACCGCCAATTTTTTACATAACACATCCGAATACAGTCTGGTGTTAAGGGTTGCTATCgg                                                                                                     >  2:1897045/1‑140 (MQ=255)
                                   atcgatcgCATCTTTACCCGCGTAGGCGCGGCAGTTGTCCTGGCGTCCGGGCGCTCAACCTTTAGGGTGGAGATGACTGAAACCGCGACTATTTTACATAACACCAACGAATACAGTCTGGTGTTAAAGGATGAGATCgg                                                                                                     >  2:554092/1‑140 (MQ=255)
                                   atcgatcgCATCTTTACCCGCGTAGGCGCGGCAGCTGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTGTACATACCGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCgg                                                                                                     <  1:1192038/140‑1 (MQ=255)
                                   atcgatcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCgg                                                                                                     >  1:1457789/1‑140 (MQ=255)
                                   atcgatcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCgg                                                                                                     <  1:1841785/140‑1 (MQ=255)
                                   atcgatcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCgg                                                                                                     >  1:1457764/1‑140 (MQ=255)
                                   atcgatcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCgg                                                                                                     >  1:648409/1‑140 (MQ=255)
                                        tcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTg                                                                                                >  2:1265819/1‑140 (MQ=255)
                                        acgCAACATTACCCGCGTAGCCCCGCCAGATGACCAGGCGTCCGGGCGCTCAACCAATATCCGGCAGAAGACAGAAACCGCCCATAAAAAACATAACACCACCGAATACACACTGGTCTTAAAGGATCAGAAcgcgcgag                                                                                                >  2:185545/2‑138 (MQ=255)
                                         cgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATACCGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTgg                                                                                               >  1:198092/1‑140 (MQ=255)
                                         cgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTgg                                                                                               >  1:1315897/1‑140 (MQ=255)
                                          gCATCTTTACCCGCGTAGGCGCGGCAGTTGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGTTGACTGAAACCGCCAATATTTTACATAACACCACCGTATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGa                                                                                              <  2:925417/140‑1 (MQ=255)
                                          gCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTATGGTGTTAATGGATGAGATCGGGCGTGGa                                                                                              <  2:334841/140‑1 (MQ=255)
                                             tCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACg                                                                                           <  1:1759134/140‑1 (MQ=255)
                                              cTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGt                                                                                          <  2:888466/140‑1 (MQ=255)
                                                ttACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTCTTGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCTCCGAAGACCCTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGtac                                                                                        <  2:1653587/140‑3 (MQ=255)
                                                 tACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCa                                                                                       >  1:185924/1‑140 (MQ=255)
                                                  aCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCAc                                                                                      >  1:555597/1‑140 (MQ=255)
                                                  aCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCAc                                                                                      >  1:555443/1‑140 (MQ=255)
                                                  aCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCAc                                                                                      >  1:1381853/1‑140 (MQ=255)
                                                  aCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCAc                                                                                      <  2:639177/140‑1 (MQ=255)
                                                  aCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCAc                                                                                      <  1:727183/140‑1 (MQ=255)
                                                  aCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCAc                                                                                      <  2:32867/140‑1 (MQ=255)
                                                  aCCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCAc                                                                                      >  1:555604/1‑140 (MQ=255)
                                                      gcgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTAc                                                                                  >  2:733840/1‑140 (MQ=255)
                                                       cgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTTAGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGACCGTCCACCTACg                                                                                 <  2:914418/140‑1 (MQ=255)
                                                        gTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGa                                                                                >  1:109852/1‑140 (MQ=255)
                                                        gTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAAGATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGa                                                                                <  2:1270002/140‑1 (MQ=255)
                                                        gTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTAGGGTGGAGATGACTGAAACCGCCAATATTTTACATAAGACCCCCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGa                                                                                <  2:18759/140‑1 (MQ=255)
                                                        gTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTAAGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTATGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGa                                                                                >  1:1175914/1‑140 (MQ=255)
                                                           ggCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATgg                                                                             >  1:299772/1‑140 (MQ=255)
                                                             cgcgGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTc                                                                           <  1:851530/140‑1 (MQ=255)
                                                             cgcgGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTc                                                                           <  2:1852443/140‑1 (MQ=255)
                                                                 gCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTc                                                                       <  1:1072077/140‑1 (MQ=255)
                                                                 gCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTc                                                                       <  1:1072489/140‑1 (MQ=255)
                                                                 gCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTc                                                                       <  1:73472/140‑1 (MQ=255)
                                                                 gCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTc                                                                       <  2:1062849/140‑1 (MQ=255)
                                                                  cAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCg                                                                      <  1:1634411/140‑1 (MQ=255)
                                                                   aGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGc                                                                     <  2:1728967/140‑1 (MQ=255)
                                                                               gTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTggcgtgggcgt                                                         >  1:1574854/1‑140 (MQ=255)
                                                                                tCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTggcgtgggcgtg                                                        <  2:1873565/140‑1 (MQ=255)
                                                                                  cGGGCGCTCAACCTTTATGGTGGAGATGACTGTAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGAAGAGTTCGGGCGTGGAACGTCCACCTACGATGGTATGTCGCTGGCGTGGGAGTgcg                                                      >  2:1888867/1‑140 (MQ=255)
                                                                                  cGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTgcg                                                      >  2:1239829/1‑140 (MQ=255)
                                                                                  cGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTgcg                                                      >  1:1227895/1‑140 (MQ=255)
                                                                                  cGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTgcg                                                      >  1:337474/1‑140 (MQ=255)
                                                                                  cGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTgcg                                                      >  1:158607/1‑140 (MQ=255)
                                                                                                  aTGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGaa                                      <  2:771016/140‑1 (MQ=255)
                                                                                                  aTGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGaa                                      <  2:706850/140‑1 (MQ=255)
                                                                                                  aTGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGaa                                      <  2:706591/140‑1 (MQ=255)
                                                                                                  aTGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGaa                                      <  1:267571/140‑1 (MQ=255)
                                                                                                  aTGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGaa                                      <  2:769612/140‑1 (MQ=255)
                                                                                                  aTGGTGGAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGaa                                      <  2:770783/140‑1 (MQ=255)
                                                                                                   tggtggAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGTGTGGGCGTGCGCGGAAAATCTGGCGAAt                                     <  1:787016/140‑1 (MQ=255)
                                                                                                   tggtggAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAAt                                     <  2:312283/140‑1 (MQ=255)
                                                                                                   tggtggAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAAt                                     <  1:1293060/140‑1 (MQ=255)
                                                                                                   tggtggAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAAt                                     <  1:1290304/140‑1 (MQ=255)
                                                                                                   tggtggAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAAt                                     <  2:1500075/140‑1 (MQ=255)
                                                                                                    ggtggAGATGACTGAAACCGCCAATATTGTACATACCACCACCGCATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCAACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATa                                    <  1:1113004/140‑1 (MQ=255)
                                                                                                       ggAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGa                                 >  1:172210/1‑140 (MQ=255)
                                                                                                       ggAGATGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGa                                 >  2:55259/1‑140 (MQ=255)
                                                                                                        gagaTGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGAt                                >  2:1466673/1‑140 (MQ=255)
                                                                                                        gagaTGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGAt                                >  1:702759/1‑140 (MQ=255)
                                                                                                        gagaTGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGAt                                >  2:1004967/1‑140 (MQ=255)
                                                                                                        gagaTGACTGAAACCGCCAATATTTTACAAAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGACCACCTACGATGCTCTGTCGCTGGCGAGGGCGAGCGCGGAAAATCTCGCCAATAAGAt                                >  2:1912939/1‑140 (MQ=255)
                                                                                                          gaTGACTGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTa                              <  1:1813141/140‑1 (MQ=255)
                                                                                                                tGAAACCGCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCAt                        >  2:75786/1‑140 (MQ=255)
                                                                                                                  aaaCCGCCAATATTTTACATAACACAACCGAATACAGTCAGGGTTAAAAGGATGAGATCGGGCGTGTAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCTAATAAGATTAAGGCAATg                      >  1:1837988/1‑140 (MQ=255)
                                                                                                                       gCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGtt                 <  2:551692/140‑1 (MQ=255)
                                                                                                                       gCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGtt                 <  2:551636/140‑1 (MQ=255)
                                                                                                                       gCCAATATTTTACATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGtt                 <  2:918964/140‑1 (MQ=255)
                                                                                                                                   cATAACACCACCGAATACAGTCTGGTTATAATGGAAGACATCGGTCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAAACTGGCGAATAACATTAACGCATTGACGTTATTTGCTACCCa     <  2:322852/140‑1 (MQ=255)
                                                                                                                                   cATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCTCCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAATATTAAGGCATTGACGTTATTTGATACCCa     <  1:1013201/140‑1 (MQ=255)
                                                                                                                                   cATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTCCGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAACTCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCa     <  1:939868/140‑1 (MQ=255)
                                                                                                                                   cATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCa     <  2:310532/140‑1 (MQ=255)
                                                                                                                                   cATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCa     <  2:311953/140‑1 (MQ=255)
                                                                                                                                   cATAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCa     <  2:1799513/140‑1 (MQ=255)
                                                                                                                                   aaTAACACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAACGCATTGACGTTATTTGCTACCCa     <  2:322705/139‑1 (MQ=255)
                                                                                                                                      aaCACCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTa  >  2:1824771/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCTACGATGGTCTGTCGCTGGCGTGGGCGTGCGCGGAAAATCTGGCGAATAAGATTAAGGCATTGACGTTATTTGCTACCCACTA  >  NC_000913/2859005‑2859278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: