Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,116,386 C→A 5.2% A909A (GCG→GCT sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,116,3860CA 5.2% 425.6 / 6.5 116A909A (GCG→GCTsslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (41/69);  new base A (3/3);  total (44/72)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 116 total aligned reads displayed.

AGACCGACATGTTCTGGTACAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTAGTACA  >  NC_000913/3116250‑3116517
                                                                                                                                        |                                                                                                                                   
aGACCGACATGTTCTGGTACAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCt                                                                                                                                  >  2:795836/1‑140 (MQ=255)
aGACCGACATGTTCTGGTACAGACGTTACAGATAGACGCTGCTCAGACGCTCACCTTTGCGACCATTTGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATACGCAGCCAGCACCAACGCt                                                                                                                                  >  1:500968/1‑140 (MQ=255)
      aCATGTTCTGGTACAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCa                                                                                                                            >  1:343711/1‑140 (MQ=255)
            tCTGGTACAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCg                                                                                                                      >  2:1672265/1‑140 (MQ=255)
                gTACAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGt                                                                                                                  >  2:712958/1‑140 (MQ=255)
                 tACAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGtt                                                                                                                 >  2:523143/1‑140 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  1:1069791/140‑1 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  2:141914/140‑1 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  1:878523/140‑1 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  1:233381/140‑1 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  1:1445085/140‑1 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  1:1416901/140‑1 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  1:1036007/140‑1 (MQ=255)
                  aCAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGAACCATCGCTTTTGCAGTGTCGGCGTTa                                                                                                                <  1:1821373/140‑1 (MQ=255)
                     gACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAgg                                                                                                             <  2:1594401/140‑1 (MQ=255)
                       cGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCt                                                                                                           <  2:573552/140‑1 (MQ=255)
                         ttCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTTGTCCGGAAGTAGAGCTCATGCAGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         <  2:1903668/140‑1 (MQ=255)
                         ttCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         <  2:910460/140‑1 (MQ=255)
                         ttCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         <  2:695406/140‑1 (MQ=255)
                         ttCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         <  2:198232/140‑1 (MQ=255)
                         ttCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         <  2:1904330/140‑1 (MQ=255)
                         ttCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         <  1:483464/140‑1 (MQ=255)
                          tCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         >  2:1209645/1‑139 (MQ=255)
                          tCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTa                                                                                                         <  1:1209645/139‑1 (MQ=255)
                          tCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAg                                                                                                        >  2:822087/1‑140 (MQ=255)
                           ccAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGt                                                                                                       <  1:1206384/140‑1 (MQ=255)
                            cAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGtt                                                                                                      <  2:323772/140‑1 (MQ=255)
                                 cGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGta                                                                                                 <  1:499392/140‑1 (MQ=255)
                                 cGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGta                                                                                                 <  1:1567519/140‑1 (MQ=255)
                                   aCGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGtata                                                                                               <  1:1796960/140‑1 (MQ=255)
                                   aCGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGtata                                                                                               >  2:476803/1‑140 (MQ=255)
                                     gctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACt                                                                                             <  1:1197914/140‑1 (MQ=255)
                                     gctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACt                                                                                             <  1:449971/140‑1 (MQ=255)
                                     gctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACt                                                                                             <  1:590554/140‑1 (MQ=255)
                                      ctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACtg                                                                                            <  1:324896/140‑1 (MQ=255)
                                      ctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACtg                                                                                            <  1:1101100/140‑1 (MQ=255)
                                      ctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATAGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACtg                                                                                            >  2:893744/1‑140 (MQ=255)
                                      ctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATAGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACtg                                                                                            >  2:894008/1‑140 (MQ=255)
                                      ctgctCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATAGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACtg                                                                                            >  2:894019/1‑140 (MQ=255)
                                          tCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGa                                                                                        <  1:1490085/140‑1 (MQ=255)
                                              aCGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAacat                                                                                    >  2:405436/1‑140 (MQ=255)
                                              aCGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAacat                                                                                    >  2:1600207/1‑140 (MQ=255)
                                              aCGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAacat                                                                                    >  1:346611/1‑140 (MQ=255)
                                              aCGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAacat                                                                                    >  2:1441927/1‑140 (MQ=255)
                                              aCGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGATGAATGTTGGTGCCTAAATCCGCAGCCTGCACCAGCGCTTTTGGAGTGTCGGCGTTATGGCGTACTTGCGTATACTGAGGAacat                                                                                    >  2:1910570/1‑140 (MQ=255)
                                               cGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAacata                                                                                   <  2:80694/140‑1 (MQ=255)
                                               cGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAacata                                                                                   <  2:1121322/140‑1 (MQ=255)
                                                 cTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAacataca                                                                                 >  1:1877983/1‑140 (MQ=255)
                                                   cACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATg                                                                               >  2:151941/1‑140 (MQ=255)
                                                   cACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATg                                                                               >  1:1864517/1‑140 (MQ=255)
                                                          gCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAg                                                                        >  2:1643175/1‑140 (MQ=255)
                                                            gACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTa                                                                      >  1:1872065/1‑140 (MQ=255)
                                                              ccATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAAc                                                                    <  1:1437266/140‑1 (MQ=255)
                                                                      ccGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAAccc                                                            <  1:1711454/140‑1 (MQ=255)
                                                                       cGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAAcccc                                                           <  1:1310713/140‑1 (MQ=255)
                                                                       cGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAAcccc                                                           <  1:1831302/140‑1 (MQ=255)
                                                                       cGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAAcccc                                                           <  1:1695254/140‑1 (MQ=255)
                                                                         gAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCg                                                         >  1:618189/1‑140 (MQ=255)
                                                                         gAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCg                                                         >  1:1589019/1‑140 (MQ=255)
                                                                            gTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGATAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGc                                                      <  2:676417/140‑1 (MQ=255)
                                                                              agagCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCa                                                    <  1:1333776/140‑1 (MQ=255)
                                                                               gagCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAg                                                   <  2:1164544/140‑1 (MQ=255)
                                                                               gagCTCATGCTGATACAGACGCAGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTAAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAg                                                   <  1:26067/140‑1 (MQ=255)
                                                                                   tCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACg                                               <  1:590854/140‑1 (MQ=255)
                                                                                           aTACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCa                                       <  2:1043739/140‑1 (MQ=255)
                                                                                           aTACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCa                                       <  2:725141/140‑1 (MQ=255)
                                                                                           aTACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCa                                       <  1:441347/140‑1 (MQ=255)
                                                                                            tACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAg                                      >  2:603259/1‑140 (MQ=255)
                                                                                            tACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAg                                      >  2:603076/1‑140 (MQ=255)
                                                                                            tACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAg                                      >  1:1504954/1‑140 (MQ=255)
                                                                                            tACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAg                                      >  1:1505029/1‑140 (MQ=255)
                                                                                               aGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCa                                   >  2:402544/1‑140 (MQ=255)
                                                                                                 aCGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGt                                 >  1:1791632/1‑140 (MQ=255)
                                                                                                 aCGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGt                                 <  2:136252/140‑1 (MQ=255)
                                                                                                  cGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAACTCTTAGTTGCGTATACTGTTGAACATTCATGCCACCATTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGtt                                <  2:1352187/140‑1 (MQ=255)
                                                                                                   gCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTg                               <  2:1237461/140‑1 (MQ=255)
                                                                                                     tGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGAc                             <  1:890508/140‑1 (MQ=255)
                                                                                                     tGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGAc                             >  1:710309/1‑140 (MQ=255)
                                                                                                     tGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGATAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGAc                             >  2:268227/1‑140 (MQ=255)
                                                                                                         tGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGAAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCa                         <  2:885924/140‑1 (MQ=255)
                                                                                                             ggTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGt                     >  1:1558841/1‑140 (MQ=255)
                                                                                                             ggTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGt                     >  1:662143/1‑140 (MQ=255)
                                                                                                               tgacTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATAATGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGTCAGTTGACCTCATAGTGa                   <  1:91028/137‑1 (MQ=255)
                                                                                                                 ccTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAaca                                                                                     >  1:945553/1‑72 (MQ=255)
                                                                                                                 ccTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGata                 >  1:161659/1‑140 (MQ=255)
                                                                                                                    aaaTCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGc              <  1:365918/140‑1 (MQ=255)
                                                                                                                    aaaTCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGc              <  1:214530/140‑1 (MQ=255)
                                                                                                                    aaaTCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGc              <  1:1760331/140‑1 (MQ=255)
                                                                                                                    aaaTCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGc              <  2:1599820/140‑1 (MQ=255)
                                                                                                                      aTCCGCAGCCTGCACCATCGCTTTTGTAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCg            <  1:187223/140‑1 (MQ=255)
                                                                                                                      aTCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCg            <  1:1167624/140‑1 (MQ=255)
                                                                                                                         cGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGAt         <  2:1112365/140‑1 (MQ=255)
                                                                                                                         cGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGAt         <  2:1116606/140‑1 (MQ=255)
                                                                                                                           cAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTa       >  1:801639/1‑140 (MQ=255)
                                                                                                                           cAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTAGACCTCATAGTGATATGCCGGATTa       >  1:1496800/1‑140 (MQ=255)
                                                                                                                               cTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTAGTac   <  1:1308700/140‑1 (MQ=255)
                                                                                                                               cTGCACCATAGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTAGTac   <  1:893744/140‑1 (MQ=255)
                                                                                                                               cTGCACCATAGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTAGTac   <  1:894008/140‑1 (MQ=255)
                                                                                                                               cTGCACCATAGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTAGTac   <  1:894019/140‑1 (MQ=255)
                                                                                                                                tGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTAGTaca  <  2:1230884/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
AGACCGACATGTTCTGGTACAGACGTTCCAGATCGACGCTGCTCAGACGCTCACCTTTGCGACCATTGGTCCGGAAGTAGAGCTCATGCTGATACAGACGCTGAATGTTGGTGCCTAAATCCGCAGCCTGCACCATCGCTTTTGCAGTGTCGGCGTTAAGGCTTAGTTGCGTATACTGTGGAACATACATGCCACCAGTAACCGGAACCCCCGTGCCAGGACGATATTCCAGGCAGTTGACCTCATAGTGATATGCCGGATTAGTACA  >  NC_000913/3116250‑3116517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: