Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,150,525	C→T	5.6%	C288C (TGC→TGT)	yghA →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,150,525	0	C	T	5.6%	250.9 / 3.9	73	C288C (TGC→TGT)	yghA	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (35/33);  new base T (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

AACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATA  >  NC_000913/3150387‑3150660                                                                                                                                           |                                                                                                                                        aaCGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTgcg                                                                                                                                        <  2:856600/140‑1 (MQ=255) aaCGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTgcg                                                                                                                                        <  2:141093/140‑1 (MQ=255)      aGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTgcggcggc                                                                                                                                   <  2:506012/140‑1 (MQ=255)              aTCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCAttt                                                                                                                           >  2:187247/1‑140 (MQ=255)              aTCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGAAAGTCAGGAGTCGAGCTACGTCACCGCAGATGTGAACGGCGTGTGCGGCGGCGAGCAttt                                                                                                                           >  2:190667/1‑140 (MQ=255)               tCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTa                                                                                                                          <  1:941361/140‑1 (MQ=255)                 ccGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAgg                                                                                                                        <  2:780614/140‑1 (MQ=255)                  cGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGTGGCGGCGAGCATTTAGGt                                                                                                                       >  2:263400/1‑140 (MQ=255)                  cGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGTGGCGGCGAGCATTTAGGt                                                                                                                       >  2:260134/1‑140 (MQ=255)                  cGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGt                                                                                                                       >  2:1101277/1‑140 (MQ=255)                  cGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGt                                                                                                                       >  2:150964/1‑140 (MQ=255)                    cAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTa                                                                                                                     <  1:108765/140‑1 (MQ=255)                         tGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTaaaaaa                                                                                                                >  1:770700/1‑140 (MQ=255)                              aGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGcc                                                                                                           <  1:1083321/140‑1 (MQ=255)                              aGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGcc                                                                                                           <  1:963218/140‑1 (MQ=255)                              aGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGcc                                                                                                           <  1:36797/140‑1 (MQ=255)                              aGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGcc                                                                                                           <  1:1815817/140‑1 (MQ=255)                                 aaaCGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCTAGTCCGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGGGCGGCGGCGAGCATTTAGGTTATATAAATGCCCgg                                                                                                        >  1:733113/1‑140 (MQ=255)                                 aaaCGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCgg                                                                                                        <  1:887030/140‑1 (MQ=255)                                 aaaCGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCgg                                                                                                        <  1:886516/140‑1 (MQ=255)                                 aaaCGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGGCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCAGTTAGGTTAAAAAAATGCCCgg                                                                                                        >  1:873453/1‑140 (MQ=255)                                 aaaCGCCGATGAAACGAGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCgg                                                                                                        <  1:887042/140‑1 (MQ=255)                                    cGCCGATGAATCGTGCGGGGCATCCGGCGGAACTGGCCCCTGTATATGTGTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAATTGCCCGGTtg                                                                                                     <  2:283452/140‑1 (MQ=255)                                    cGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCTAGTCTGGATTCGAGCTAAGTCACCGCAGAAGTGCACGGAGTTTGAGTCGGCGATCATTTAGGTTAAAAATATGCCAGGttt                                                                                                     <  2:1074987/140‑2 (MQ=255)                                     gCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTtgt                                                                                                    >  2:423911/1‑140 (MQ=255)                                         aTGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGaaa                                                                                                <  2:405994/140‑1 (MQ=255)                                                 tGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCg                                                                                        >  2:1104595/1‑140 (MQ=255)                                                          aCCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCAtt                                                                               <  1:1767776/140‑1 (MQ=255)                                                          aCCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCAtt                                                                               <  1:809015/140‑1 (MQ=255)                                                           ccGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATtg                                                                              >  2:1479248/1‑140 (MQ=255)                                                            cggcggAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATtgt                                                                             <  1:1320678/140‑1 (MQ=255)                                                                ggAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAAt                                                                         <  1:550691/140‑1 (MQ=255)                                                                 gAACTGGCCACTGTATTAGTTTTTCTGGCAAGTCAGTAGTCGAGATACGTCATCGCAGAATTGCAAGTCGTGTTCGGCGGCGATCTTTTAGGTTAAAAAAATGCGCGGTTGTGAAAAGCTACCTGGCATAATTGTGAAtt                                                                        <  2:1139887/140‑1 (MQ=255)                                                                   aCTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCa                                                                      <  1:295439/140‑1 (MQ=255)                                                                   aCTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCa                                                                      <  1:295366/140‑1 (MQ=255)                                                                   aCTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCa                                                                      <  1:1637039/140‑1 (MQ=255)                                                                     tGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCAct                                                                    <  1:1723897/140‑1 (MQ=255)                                                                       gCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCActct                                                                  >  1:10694/1‑140 (MQ=255)                                                                            tGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCt                                                             >  1:581487/1‑140 (MQ=255)                                                                                 atGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGAtgt                                                        >  1:1048334/1‑140 (MQ=255)                                                                                 atGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGAtgt                                                        >  1:1548469/1‑140 (MQ=255)                                                                                      tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAATAGCAACCGGGCATCATTGTGAATTCAct                                                                    <  1:1403735/123‑1 (MQ=255)                                                                                      tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCAct                                                                    >  2:1407044/1‑123 (MQ=255)                                                                                      tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCAct                                                                    <  1:1407044/123‑1 (MQ=255)                                                                                      tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCAct                                                                    >  2:1403735/1‑123 (MQ=255)                                                                                      tATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGa                                                   >  1:203818/1‑140 (MQ=255)                                                                                        tCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCGCTCTTACCTGATGTGGCGaaa                                                 >  1:1362252/1‑140 (MQ=255)                                                                                        tCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGaaa                                                 >  1:430099/1‑140 (MQ=255)                                                                                          tGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAAc                                               <  1:1719103/140‑1 (MQ=255)                                                                                            gCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGc                                             >  2:1068767/1‑140 (MQ=255)                                                                                             cAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGTGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGcc                                            <  1:260134/140‑1 (MQ=255)                                                                                             cAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGTGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGcc                                            <  1:263400/140‑1 (MQ=255)                                                                                                   aGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATcc                                      >  1:1579499/1‑140 (MQ=255)                                                                                                   aGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATcc                                      >  1:182005/1‑140 (MQ=255)                                                                                                   aGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGAGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATcc                                      >  1:1579572/1‑140 (MQ=255)                                                                                                          gAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTAGCTGATGTGGCGAAATCGGCTTATCCGCCTACa                               >  1:1030656/1‑140 (MQ=255)                                                                                                          gAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACa                               <  2:1228357/140‑1 (MQ=255)                                                                                                              tACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACg                           <  1:994789/140‑1 (MQ=255)                                                                                                              tACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACg                           <  1:509304/140‑1 (MQ=255)                                                                                                                  tCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATg                       <  2:1837046/140‑1 (MQ=255)                                                                                                                  tCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATg                       <  2:1837027/140‑1 (MQ=255)                                                                                                                  tCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATg                       <  2:1836994/140‑1 (MQ=255)                                                                                                                  acACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGCTAAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATAGTGAATTCACTCTTACCTGATGTCGCGAAAACGCCTTATCCGCCTACAAACGCATg                       <  2:77098/139‑1 (MQ=255)                                                                                                                          gAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCa               >  2:1098615/1‑140 (MQ=255)                                                                                                                          gAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGGGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTct               >  2:691054/1‑139 (MQ=255)                                                                                                                             gTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACa            >  2:1327129/1‑140 (MQ=255)                                                                                                                             gTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACa            >  2:1005194/1‑140 (MQ=255)                                                                                                                             gTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACa            >  2:1005271/1‑140 (MQ=255)                                                                                                                                cACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGt         <  2:937534/140‑1 (MQ=255)                                                                                                                                cACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCAGTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCGTATCCGCCTACAAACGCATGAAGATTCAACATGt         >  2:1922714/1‑140 (MQ=255)                                                                                                                                cACGGCGTGTGCGGCGGCGAGCATGTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGt         >  2:659574/1‑140 (MQ=255)                                                                                                                                    gCGTGTGCTGCGGCGAGCATTTAGGTTAAAAAATTGCCCGGTTGTTAAAAGCAACCGGGCATCATTGTGAATTAAATCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCa     <  1:854712/140‑1 (MQ=39)                                                                                                                                     cGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCaa    >  1:420509/1‑140 (MQ=255)                                                                                                                                     cGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCAGTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCaa    >  1:416925/1‑140 (MQ=255)                                                                                                                                       tgtgCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATa  >  1:690994/1‑140 (MQ=255)                                                                                                                                       tgtgCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATa  >  1:933790/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        AACGCAGGATAAGATCCCGCAGTTTGGTCAGCAAACGCCGATGAAACGTGCGGGGCAACCGGCGGAACTGGCCCCTGTATATGTTTATCTGGCAAGTCAGGAGTCGAGCTACGTCACCGCAGAAGTGCACGGCGTGTGCGGCGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATA  >  NC_000913/3150387‑3150660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑