Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,299,293 C→A 5.2% P107Q (CCG→CAG)  yhbO → stress‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,299,2930CA 5.2% 276.3 / 2.3 77P107Q (CCG→CAG) yhbOstress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base A (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

ACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTAC  >  NC_000913/3299157‑3299428
                                                                                                                                        |                                                                                                                                       
aCGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGTATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCa                                                                                                                                      >  1:1662974/1‑140 (MQ=255)
 cGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCAGTCACCGGCTTATCTGCGTGGGGTCACCCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAg                                                                                                                                     <  2:1274111/140‑1 (MQ=255)
     tGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGc                                                                                                                                 >  2:1752185/1‑140 (MQ=255)
     tGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGc                                                                                                                                 >  1:602723/1‑140 (MQ=255)
     tGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCAGCAGTTGc                                                                                                                                 >  1:1312893/1‑140 (MQ=255)
     tGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCAGCAGTTGc                                                                                                                                 >  1:1312880/1‑140 (MQ=255)
        ggAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGa                                                                                                                              >  1:492560/1‑140 (MQ=255)
         gAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGAt                                                                                                                             <  2:1579708/140‑1 (MQ=255)
            tttGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAg                                                                                                                          >  2:1502326/1‑140 (MQ=255)
              tGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAgcg                                                                                                                        >  1:1432710/1‑140 (MQ=255)
                 tGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCg                                                                                                                     <  2:785826/140‑1 (MQ=255)
                  gCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGa                                                                                                                    <  1:1790923/140‑1 (MQ=255)
                  gCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTAACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGa                                                                                                                    <  2:1114581/140‑1 (MQ=255)
                   cccTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAt                                                                                                                   >  1:1558629/1‑140 (MQ=255)
                   cccTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAt                                                                                                                   >  1:1559119/1‑140 (MQ=255)
                    ccTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAtg                                                                                                                  <  1:178007/140‑1 (MQ=255)
                      tgctgcTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAtgtg                                                                                                                >  1:1265016/1‑140 (MQ=255)
                      tgctgcTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGAtgtg                                                                                                                >  2:1188069/1‑140 (MQ=255)
                      tgcagcTACCGGGCGGCCATTCAACGGATTATCAGCGTGGGGACAAGCGTTTTGTCACCTTTACCCGTGATTTTGTGAATTTTGGCTCACCGGTGTTTGCCATCTGTCACGTCCCGCAGTTGCTGATCAGCGCCGAtgtg                                                                                                                >  2:1188068/1‑140 (MQ=255)
                                  gcggcCATTCACCGTATTATCTGCGTGGGGACAAACGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGGTGCTGAGCAGCGCCGATGTGATTCGGGGgcgc                                                                                                    >  2:344210/1‑140 (MQ=255)
                                  gcggcCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGgcgc                                                                                                    >  1:1566624/1‑140 (MQ=255)
                                  gcggcCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGgcgc                                                                                                    >  1:50652/1‑140 (MQ=255)
                                  gcggcCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGgcgc                                                                                                    >  1:1566679/1‑140 (MQ=255)
                                          tCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCTGGGGCGCAAACTGAc                                                                                            <  1:1068152/140‑1 (MQ=255)
                                          tCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            <  1:1538391/140‑1 (MQ=255)
                                          tCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            <  1:1477215/140‑1 (MQ=255)
                                          tCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            <  1:416799/140‑1 (MQ=255)
                                          tCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            <  1:1075791/140‑1 (MQ=255)
                                          tCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            <  1:1008649/140‑1 (MQ=255)
                                          tCACCGGATTATCTGCGTGGGGACAAACGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            <  1:746111/140‑1 (MQ=255)
                                                gATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGt                                                                                      >  2:636229/1‑140 (MQ=255)
                                                gATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGt                                                                                      >  2:1671656/1‑140 (MQ=255)
                                                gATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGt                                                                                      >  2:49419/1‑140 (MQ=255)
                                                gATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGt                                                                                      >  2:49071/1‑140 (MQ=255)
                                                      cTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCTAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAAcc                                                                                <  2:1874244/140‑1 (MQ=255)
                                                      cTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAAcc                                                                                <  2:1269936/140‑1 (MQ=255)
                                                      cTGCGAGGGGACACCCGTTTTGTCACCTTTACCCGAGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAAcc                                                                                <  2:1872273/140‑1 (MQ=255)
                                                       tGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCg                                                                               <  1:914502/140‑1 (MQ=255)
                                                        gCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGa                                                                              >  2:1442684/1‑140 (MQ=255)
                                                         cGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGAt                                                                             <  1:1250092/140‑1 (MQ=255)
                                                         cGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGAt                                                                             <  1:1333409/140‑1 (MQ=255)
                                                         cGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGAt                                                                             <  1:1784383/140‑1 (MQ=255)
                                                           tGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCa                                                                           >  2:598953/1‑140 (MQ=255)
                                                           tGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCa                                                                           >  2:782294/1‑140 (MQ=255)
                                                           tGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCa                                                                           >  2:598958/1‑140 (MQ=255)
                                                               gACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCattat                                                                       >  2:1120741/1‑140 (MQ=255)
                                                                aCAACCGTTTTGTCACGTTTACCCGTGATTTTGGGAATAGTGGCAAACCGGTGTTTGCCATCTGACACGGCCCGCAGATGCTGATCAGCGCCGATGTGTTTCGGGGGCGCAAACTGACCGCAGTTAAACCGGTCattagt                                                                      >  2:1613054/1‑138 (MQ=255)
                                                                aCAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCTTCTCTCACGGCCCGCAGAAGCTGATCAGCGCCGAGGTGATTCGGGGGCGCAAACTGTCCGGAGGTAAACCGAGCaggagt                                                                      >  2:1613053/1‑135 (MQ=255)
                                                                aCAACCGTTTTGTCACCTTTACCCGGGATTTTGTGAATAGTGGCACACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGGTAAACCGATCattatt                                                                      >  2:523031/1‑140 (MQ=255)
                                                                  accccttttGTCACCTTGACCCGCGATTTAGTGAATTGTGGCAAACCGGTGATTGCCATCTGTCACGGCCCGCAGTTGCAGATCAGCTCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGa                                                                    <  2:433285/135‑1 (MQ=255)
                                                                  aaCCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGa                                                                    <  2:436344/140‑1 (MQ=255)
                                                                  aaCCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGa                                                                    <  2:1137627/140‑1 (MQ=255)
                                                                  aaCCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGa                                                                    <  2:1138269/140‑1 (MQ=255)
                                                                   aCCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGAt                                                                   >  1:479709/1‑140 (MQ=255)
                                                                       ttttGTCACCTTAACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTa                                                               >  2:353987/1‑140 (MQ=255)
                                                                          tGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTaaaa                                                            >  2:382769/1‑140 (MQ=255)
                                                                                 tttACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            >  2:901720/1‑101 (MQ=255)
                                                                                 tttACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGAc                                                                                            <  1:901720/101‑1 (MQ=255)
                                                                                          gATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTTATGTTAAAAATGCGGGCGCGGAAta                                            >  1:1887991/1‑139 (MQ=255)
                                                                                          gATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAAtt                                            <  2:265428/140‑1 (MQ=255)
                                                                                             tttGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTa                                         <  2:1820256/140‑1 (MQ=255)
                                                                                             tttGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTa                                         <  2:793/140‑1 (MQ=255)
                                                                                               tgtgAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACg                                       <  1:12711/140‑1 (MQ=255)
                                                                                                gtgAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGa                                      <  1:551201/140‑1 (MQ=255)
                                                                                                      aGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGa                                >  2:361518/1‑140 (MQ=255)
                                                                                                          gCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAATCCGATCATTATTGATGTTAAAATTGCGGGCGCGGAATTTTACGATCAGGAAGTc                            >  1:391131/1‑140 (MQ=255)
                                                                                                          gCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTc                            >  1:1011686/1‑140 (MQ=255)
                                                                                                          gCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTc                            <  2:722907/140‑1 (MQ=255)
                                                                                                          gCAAACCGGTGTTTGCCATCTGTCACGGCCAGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTc                            <  2:1312880/140‑1 (MQ=255)
                                                                                                          gCAAACCGGTGTTTGCCATCTGTCACGGCCAGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTc                            <  2:1312893/140‑1 (MQ=255)
                                                                                                               ccGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGt                       <  2:610618/140‑1 (MQ=255)
                                                                                                                  gtgtTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGa                    <  2:1394687/140‑1 (MQ=255)
                                                                                                                     tttGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATaa                 <  1:1014895/140‑1 (MQ=255)
                                                                                                                         ccATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGAt             >  1:671814/1‑140 (MQ=255)
                                                                                                                         ccATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGAt             >  1:725223/1‑140 (MQ=255)
                                                                                                                                    ggCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTAc  <  2:1294339/140‑1 (MQ=255)
                                                                                                                                    ggCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTAc  <  2:1295795/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ACGCCTGCGGAGTTTGATGCCCTGCTGCTACCGGGCGGCCATTCACCGGATTATCTGCGTGGGGACAACCGTTTTGTCACCTTTACCCGTGATTTTGTGAATAGTGGCAAACCGGTGTTTGCCATCTGTCACGGCCCGCAGTTGCTGATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTAC  >  NC_000913/3299157‑3299428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: