Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,449,960 G→T 6.2% R92S (CGT→AGT)  rplV ← 50S ribosomal subunit protein L22

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,449,9600GT 6.2% 354.3 / 6.8 96R92S (CGT→AGT) rplV50S ribosomal subunit protein L22
Reads supporting (aligned to +/- strand):  ref base G (38/52);  new base T (3/3);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAC  >  NC_000913/3449822‑3450095
                                                                                                                                          |                                                                                                                                       
gTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGa                                                                                                                                        >  1:785420/1‑140 (MQ=255)
gTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGa                                                                                                                                        >  1:784414/1‑140 (MQ=255)
     gTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCttt                                                                                                                                   >  1:1706696/1‑140 (MQ=255)
     gTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCttt                                                                                                                                   >  1:1705443/1‑140 (MQ=255)
         cATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCa                                                                                                                               <  2:1379636/140‑1 (MQ=255)
          aTGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                                                                                              >  1:503921/1‑140 (MQ=255)
          aTGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                                                                                              <  2:718259/140‑1 (MQ=255)
          aTGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                                                                                              >  1:220528/1‑140 (MQ=255)
           tGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcg                                                                                                                             <  1:120993/140‑1 (MQ=255)
           tGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcg                                                                                                                             <  1:1245782/140‑1 (MQ=255)
           tGGTTTTACAATACCCAGGCGAATACCATTAGGAGGTACTTTCTGACCCATTGCTAGTCTCCAGTGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcg                                                                                                                             <  1:686592/140‑1 (MQ=255)
             gTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcgcg                                                                                                                           <  1:839224/140‑1 (MQ=255)
                   ccaTACCCCGGCGAATACCACTAGGCTGAACTTTCTGCCCCATTGCCAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGTTGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATaa                                                                                                                     <  2:21188/138‑1 (MQ=255)
                        cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                >  2:558023/1‑140 (MQ=255)
                        cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                >  1:13502/1‑140 (MQ=255)
                        cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                >  2:560175/1‑140 (MQ=255)
                        cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                >  1:1094629/1‑140 (MQ=255)
                         ccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCt                                                                                                               <  2:1642409/140‑1 (MQ=255)
                            ggCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCa                                                                                                            <  2:1566018/140‑1 (MQ=255)
                             gCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCAt                                                                                                           >  2:1817740/1‑140 (MQ=255)
                                aaTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCt                                                                                                        >  1:895028/1‑140 (MQ=255)
                                aaTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCt                                                                                                        >  1:895220/1‑140 (MQ=255)
                                aaTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCt                                                                                                        >  1:114615/1‑140 (MQ=255)
                                 aTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTc                                                                                                       <  1:10611/140‑1 (MQ=255)
                                 aTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTc                                                                                                       >  1:1539945/1‑140 (MQ=255)
                                  tACCATTAGGATGTCCTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGTTCGGACTCAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCCCGTCCTTTTGCTCGCGGCAGAATGCGCTTCATGcgcg                                                                                                      >  2:1683233/1‑140 (MQ=255)
                                  tACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCg                                                                                                      >  2:1405522/1‑140 (MQ=255)
                                               tACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTAc                                                                                         >  1:1472743/1‑140 (MQ=255)
                                               tACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCAGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCATCGTCTAc                                                                                         >  2:589397/1‑140 (MQ=255)
                                               tACCTTCTTACCCATTGCTAGTCACCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTTGCTGGTGCGCTTAAGGATGCGATCTGCACGACCGTGTGCACGCCGCATAATGCGCTTCATGCTCGGGCCGACGTCTAc                                                                                         >  2:589396/1‑140 (MQ=255)
                                                    tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                    >  1:871486/1‑140 (MQ=255)
                                                    tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                    >  1:1708188/1‑140 (MQ=255)
                                                    tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                    >  1:1708285/1‑140 (MQ=255)
                                                    tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                    >  2:503121/1‑140 (MQ=255)
                                                    tCTGACCCATTGCTACACTCCAGAGTCTCAGCGATCGGACACAACCACAGAGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTATGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                    >  1:770149/1‑140 (MQ=255)
                                                       gACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAttt                                                                                 >  2:1263662/1‑140 (MQ=255)
                                                       gACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAttt                                                                                 >  2:1263678/1‑140 (MQ=255)
                                                       gACCCATTGCTAGTCTCCAGAGTCCCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAttt                                                                                 >  2:1264501/1‑140 (MQ=255)
                                                           cATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGt                                                                             <  1:272669/140‑1 (MQ=255)
                                                           cATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGt                                                                             <  1:1817740/140‑1 (MQ=255)
                                                              tGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAAc                                                                          >  1:1411564/1‑140 (MQ=255)
                                                                   gTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCa                                                                     >  2:962488/1‑140 (MQ=255)
                                                                     ctcCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGa                                                                   <  1:1010476/140‑1 (MQ=255)
                                                                     ctcCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGa                                                                   <  1:1395457/140‑1 (MQ=255)
                                                                     ctcCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGa                                                                   <  1:661888/140‑1 (MQ=255)
                                                                          gagTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTc                                                              <  1:1263662/140‑1 (MQ=255)
                                                                          gagTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTc                                                              <  1:1263678/140‑1 (MQ=255)
                                                                          gagTCCCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTc                                                              <  1:1264501/140‑1 (MQ=255)
                                                                           agTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCa                                                             <  2:936611/140‑1 (MQ=255)
                                                                           agTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCa                                                             <  2:503865/140‑1 (MQ=255)
                                                                             tctcAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAAt                                                           >  1:60921/1‑140 (MQ=255)
                                                                               tcAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTACATGCTCGGGCCTTCGTCTACGAAAATTTTCGAAACTGTCAGATCGTCAATGt                                                         >  2:649278/1‑140 (MQ=255)
                                                                                ccgCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGTCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTc                                                        <  2:1381147/138‑1 (MQ=255)
                                                                                cAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTc                                                        >  2:674774/1‑140 (MQ=255)
                                                                                  gCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAg                                                      <  1:1440941/140‑1 (MQ=255)
                                                                                  gCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAg                                                      <  1:1440969/140‑1 (MQ=255)
                                                                                  gCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAg                                                      <  1:1546938/140‑1 (MQ=255)
                                                                                     aTCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAgcgc                                                   <  1:482226/140‑1 (MQ=255)
                                                                                       cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCa                                                 <  1:346909/140‑1 (MQ=255)
                                                                                       cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCa                                                 <  1:346768/140‑1 (MQ=255)
                                                                                       cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCa                                                 <  1:756252/140‑1 (MQ=255)
                                                                                       cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGAGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTAAATGTCAGCGCCa                                                 <  1:1080046/140‑1 (MQ=255)
                                                                                           cacaACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGt                                             <  1:245415/140‑1 (MQ=255)
                                                                                            acaACCACAGTGATGTGGCTGGTGCGCTTCAGGAAGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGtt                                            <  2:1660759/140‑1 (MQ=255)
                                                                                             caACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTtg                                           <  2:1077498/140‑1 (MQ=255)
                                                                                               aCCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTtgtg                                         >  1:762729/1‑140 (MQ=255)
                                                                                                 cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                       <  2:762729/140‑1 (MQ=255)
                                                                                                 cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                       <  1:63849/140‑1 (MQ=255)
                                                                                                 cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                       <  1:92280/140‑1 (MQ=255)
                                                                                                 cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                       <  1:1465104/140‑1 (MQ=255)
                                                                                                    aGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAg                                    >  1:664098/1‑140 (MQ=255)
                                                                                                      tGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCg                                  <  2:789702/140‑1 (MQ=255)
                                                                                                        aTGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCCATGTCAGCGCCATCGTTGTGTTCAGCGtt                                >  2:852735/1‑140 (MQ=255)
                                                                                                        aTGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGtt                                >  2:1050750/1‑140 (MQ=255)
                                                                                                          gtgGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAg                              <  2:4083/140‑1 (MQ=255)
                                                                                                            ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                            <  2:1378284/140‑1 (MQ=255)
                                                                                                            ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                            <  2:531877/140‑1 (MQ=255)
                                                                                                            ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                            <  2:1164686/140‑1 (MQ=255)
                                                                                                            ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                            <  2:1378233/140‑1 (MQ=255)
                                                                                                            ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                            <  2:1418795/140‑1 (MQ=255)
                                                                                                                  tGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCa                      >  2:45013/1‑140 (MQ=255)
                                                                                                                       ttCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCTTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTc                 >  1:648165/1‑140 (MQ=255)
                                                                                                                           ggATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGa             <  1:190798/140‑1 (MQ=255)
                                                                                                                            gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGaa            <  2:555506/140‑1 (MQ=255)
                                                                                                                            gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGaa            <  1:871204/140‑1 (MQ=255)
                                                                                                                            gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGaa            <  1:279285/140‑1 (MQ=255)
                                                                                                                                cGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACttt        >  2:1353460/1‑140 (MQ=255)
                                                                                                                                cGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACttt        >  2:1118088/1‑140 (MQ=255)
                                                                                                                                 gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc       <  1:920707/140‑1 (MQ=255)
                                                                                                                                 gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc       <  1:530863/140‑1 (MQ=255)
                                                                                                                                 gATCTGCACGACCTTTTGCACGCGGCATAATGAGCTTCATGGTCGGGACTTCGTCTACGATAATTTTCGTAACTTTCAGATAGTCAATGTCAGCGCCATCGTTGTGTTCAGCTTTAGCTATGGCAGATTCCAGAACTTTc       <  1:18768/140‑1 (MQ=255)
                                                                                                                                      gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc  <  1:760974/140‑1 (MQ=255)
                                                                                                                                      gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc  <  2:959057/140‑1 (MQ=255)
                                                                                                                                      gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc  <  2:959034/140‑1 (MQ=255)
                                                                                                                                      gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc  <  1:863465/140‑1 (MQ=255)
                                                                                                                                      gCACGACCTTTTGCACGCGGCATAATGCGCTACATGCTCGGGCCTTCGTCTACGAAAATTATCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc  <  2:959146/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAC  >  NC_000913/3449822‑3450095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: