Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,449,971 C→T 6.8% R88H (CGT→CAT)  rplV ← 50S ribosomal subunit protein L22

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,449,9710CT 6.8% 299.3 / 9.2 88R88H (CGT→CAT) rplV50S ribosomal subunit protein L22
Reads supporting (aligned to +/- strand):  ref base C (35/47);  new base T (3/3);  total (38/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

ATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCG  >  NC_000913/3449832‑3450106
                                                                                                                                           |                                                                                                                                       
aTGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                                                                                                         <  2:718259/140‑1 (MQ=255)
aTGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                                                                                                         >  1:220528/1‑140 (MQ=255)
aTGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAc                                                                                                                                         >  1:503921/1‑140 (MQ=255)
 tGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcg                                                                                                                                        <  1:120993/140‑1 (MQ=255)
 tGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcg                                                                                                                                        <  1:1245782/140‑1 (MQ=255)
 tGGTTTTACAATACCCAGGCGAATACCATTAGGAGGTACTTTCTGACCCATTGCTAGTCTCCAGTGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcg                                                                                                                                        <  1:686592/140‑1 (MQ=255)
   gTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcgcg                                                                                                                                      <  1:839224/140‑1 (MQ=255)
         ccaTACCCCGGCGAATACCACTAGGCTGAACTTTCTGCCCCATTGCCAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGTTGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATaa                                                                                                                                <  2:21188/138‑1 (MQ=255)
              cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                           >  1:1094629/1‑140 (MQ=255)
              cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                           >  2:558023/1‑140 (MQ=255)
              cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                           >  1:13502/1‑140 (MQ=255)
              cccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATgcgc                                                                                                                           >  2:560175/1‑140 (MQ=255)
               ccAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCt                                                                                                                          <  2:1642409/140‑1 (MQ=255)
                  ggCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCa                                                                                                                       <  2:1566018/140‑1 (MQ=255)
                   gCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCAt                                                                                                                      >  2:1817740/1‑140 (MQ=255)
                      aaTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCt                                                                                                                   >  1:114615/1‑140 (MQ=255)
                      aaTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCt                                                                                                                   >  1:895220/1‑140 (MQ=255)
                      aaTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCt                                                                                                                   >  1:895028/1‑140 (MQ=255)
                       aTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTc                                                                                                                  <  1:10611/140‑1 (MQ=255)
                       aTACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTc                                                                                                                  >  1:1539945/1‑140 (MQ=255)
                        tACCATTAGGATGTCCTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGTTCGGACTCAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCCCGTCCTTTTGCTCGCGGCAGAATGCGCTTCATGcgcg                                                                                                                 >  2:1683233/1‑140 (MQ=255)
                        tACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCg                                                                                                                 >  2:1405522/1‑140 (MQ=255)
                                     tACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTAc                                                                                                    >  1:1472743/1‑140 (MQ=255)
                                     tACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCAGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCATCGTCTAc                                                                                                    >  2:589397/1‑140 (MQ=255)
                                     tACCTTCTTACCCATTGCTAGTCACCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTTGCTGGTGCGCTTAAGGATGCGATCTGCACGACCGTGTGCACGCCGCATAATGCGCTTCATGCTCGGGCCGACGTCTAc                                                                                                    >  2:589396/1‑140 (MQ=255)
                                          tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                               >  1:1708188/1‑140 (MQ=255)
                                          tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                               >  1:871486/1‑140 (MQ=255)
                                          tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                               >  2:503121/1‑140 (MQ=255)
                                          tCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                               >  1:1708285/1‑140 (MQ=255)
                                          tCTGACCCATTGCTACACTCCAGAGTCTCAGCGATCGGACACAACCACAGAGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTATGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGaaaa                                                                                               >  1:770149/1‑140 (MQ=255)
                                             gACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAttt                                                                                            >  2:1263678/1‑140 (MQ=255)
                                             gACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAttt                                                                                            >  2:1263662/1‑140 (MQ=255)
                                             gACCCATTGCTAGTCTCCAGAGTCCCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAttt                                                                                            >  2:1264501/1‑140 (MQ=255)
                                                 cATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGt                                                                                        <  1:272669/140‑1 (MQ=255)
                                                 cATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGt                                                                                        <  1:1817740/140‑1 (MQ=255)
                                                    tGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAAc                                                                                     >  1:1411564/1‑140 (MQ=255)
                                                         gTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCa                                                                                >  2:962488/1‑140 (MQ=255)
                                                           ctcCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGa                                                                              <  1:661888/140‑1 (MQ=255)
                                                           ctcCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGa                                                                              <  1:1010476/140‑1 (MQ=255)
                                                           ctcCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGa                                                                              <  1:1395457/140‑1 (MQ=255)
                                                                gagTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTc                                                                         <  1:1263662/140‑1 (MQ=255)
                                                                gagTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTc                                                                         <  1:1263678/140‑1 (MQ=255)
                                                                gagTCCCAGCGATCGGACACAACCACAGTGATGTGGCTGGTTCGCTTCAGGATGCGATCTGCACTACCTTTTGCATGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTc                                                                         <  1:1264501/140‑1 (MQ=255)
                                                                 agTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCa                                                                        <  2:936611/140‑1 (MQ=255)
                                                                 agTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCa                                                                        <  2:503865/140‑1 (MQ=255)
                                                                   tctcAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAAt                                                                      >  1:60921/1‑140 (MQ=255)
                                                                     tcAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTACATGCTCGGGCCTTCGTCTACGAAAATTTTCGAAACTGTCAGATCGTCAATGt                                                                    >  2:649278/1‑140 (MQ=255)
                                                                      ccgCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGTCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTc                                                                   <  2:1381147/138‑1 (MQ=255)
                                                                      cAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTc                                                                   >  2:674774/1‑140 (MQ=255)
                                                                        gCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAg                                                                 <  1:1546938/140‑1 (MQ=255)
                                                                        gCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAg                                                                 <  1:1440941/140‑1 (MQ=255)
                                                                        gCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAg                                                                 <  1:1440969/140‑1 (MQ=255)
                                                                           aTCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAgcgc                                                              <  1:482226/140‑1 (MQ=255)
                                                                             cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCa                                                            <  1:756252/140‑1 (MQ=255)
                                                                             cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCa                                                            <  1:346768/140‑1 (MQ=255)
                                                                             cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCa                                                            <  1:346909/140‑1 (MQ=255)
                                                                             cGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGAGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTAAATGTCAGCGCCa                                                            <  1:1080046/140‑1 (MQ=255)
                                                                                 cacaACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGt                                                        <  1:245415/140‑1 (MQ=255)
                                                                                  acaACCACAGTGATGTGGCTGGTGCGCTTCAGGAAGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGtt                                                       <  2:1660759/140‑1 (MQ=255)
                                                                                   caACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTtg                                                      <  2:1077498/140‑1 (MQ=255)
                                                                                     aCCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTtgtg                                                    >  1:762729/1‑140 (MQ=255)
                                                                                       cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                                  <  1:92280/140‑1 (MQ=255)
                                                                                       cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                                  <  1:63849/140‑1 (MQ=255)
                                                                                       cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                                  <  2:762729/140‑1 (MQ=255)
                                                                                       cacaGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGtt                                                  <  1:1465104/140‑1 (MQ=255)
                                                                                          aGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAg                                               >  1:664098/1‑140 (MQ=255)
                                                                                            tGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCg                                             <  2:789702/140‑1 (MQ=255)
                                                                                              aTGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCCATGTCAGCGCCATCGTTGTGTTCAGCGtt                                           >  2:852735/1‑140 (MQ=255)
                                                                                              aTGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGtt                                           >  2:1050750/1‑140 (MQ=255)
                                                                                                gtgGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAg                                         <  2:4083/140‑1 (MQ=255)
                                                                                                  ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                                       <  2:1164686/140‑1 (MQ=255)
                                                                                                  ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                                       <  2:531877/140‑1 (MQ=255)
                                                                                                  ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                                       <  2:1418795/140‑1 (MQ=255)
                                                                                                  ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                                       <  2:1378284/140‑1 (MQ=255)
                                                                                                  ggCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCa                                       <  2:1378233/140‑1 (MQ=255)
                                                                                                        tGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCa                                 >  2:45013/1‑140 (MQ=255)
                                                                                                             ttCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCTTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTc                            >  1:648165/1‑140 (MQ=255)
                                                                                                                 ggATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGa                        <  1:190798/140‑1 (MQ=255)
                                                                                                                  gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGaa                       <  1:279285/140‑1 (MQ=255)
                                                                                                                  gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGaa                       <  1:871204/140‑1 (MQ=255)
                                                                                                                  gATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGaa                       <  2:555506/140‑1 (MQ=255)
                                                                                                                      cGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACttt                   >  2:1118088/1‑140 (MQ=255)
                                                                                                                      cGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACttt                   >  2:1353460/1‑140 (MQ=255)
                                                                                                                       gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc                  <  1:530863/140‑1 (MQ=255)
                                                                                                                       gATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTc                  <  1:920707/140‑1 (MQ=255)
                                                                                                                       gATCTGCACGACCTTTTGCACGCGGCATAATGAGCTTCATGGTCGGGACTTCGTCTACGATAATTTTCGTAACTTTCAGATAGTCAATGTCAGCGCCATCGTTGTGTTCAGCTTTAGCTATGGCAGATTCCAGAACTTTc                  <  1:18768/140‑1 (MQ=255)
                                                                                                                            gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc             <  1:760974/140‑1 (MQ=255)
                                                                                                                            gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc             <  1:863465/140‑1 (MQ=255)
                                                                                                                            gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc             <  2:959034/140‑1 (MQ=255)
                                                                                                                            gCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc             <  2:959057/140‑1 (MQ=255)
                                                                                                                            gCACGACCTTTTGCACGCGGCATAATGCGCTACATGCTCGGGCCTTCGTCTACGAAAATTATCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGAc             <  2:959146/140‑1 (MQ=255)
                                                                                                                                    tagtGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCGACGAAAATTTTCGTAACTTTCAGTTGGTCAATTTCAGCGCCATCGTTGTGTTCAGCGTTAGCTATGGCAGATTCCAGAACTTTCTTGACCAGTACAg     <  1:1508089/137‑1 (MQ=255)
                                                                                                                                       tGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCg  >  1:178951/1‑140 (MQ=255)
                                                                                                                                       tGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCg  >  1:76765/1‑140 (MQ=255)
                                                                                                                                       tGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCg  >  1:76773/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
ATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCG  >  NC_000913/3449832‑3450106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑