Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,861 T→A 15.6% intergenic (+78/+74) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8610TA15.6% 583.6 / 15.6 300intergenic (+78/+74)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (45/180);  new base A (55/2);  total (118/182)
Fisher's exact test for biased strand distribution p-value = 3.92e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

TCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGG  >  NC_000913/3504722‑3504998
                                                                                                                                           |                                                                                                                                          
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:1678186/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:1791415/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  2:1739767/140‑1 (MQ=255)
tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:39486/140‑1 (MQ=255)
tctaCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGAGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCACt                                                                                                                                            <  2:1397113/140‑1 (MQ=255)
gctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                            <  1:1218660/139‑1 (MQ=255)
 ctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTc                                                                                                                                           >  2:1502857/1‑140 (MQ=255)
  tcCGAACTCTTCTGTCGCGCCAATCGCATCACCTTATCTATTGATAATAAAAGACATTAACCGTAGGCCGGTTAAGATGCGCAAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCa                                                                                                                                          <  2:1238335/140‑1 (MQ=255)
      taCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCt                                                                                                                                      <  2:593689/139‑1 (MQ=255)
         tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCaaaa                                                                                                                                   >  2:618347/1‑139 (MQ=255)
          cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                  <  1:1647320/140‑1 (MQ=255)
           ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAccc                                                                                                                                 >  2:1847346/1‑140 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  1:226550/140‑1 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  1:1817371/140‑1 (MQ=255)
            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                <  2:1339779/140‑1 (MQ=255)
              tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctct                                                                                                                             <  2:1678434/140‑1 (MQ=255)
              tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctct                                                                                                                             <  2:395623/140‑1 (MQ=255)
                   cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCa                                                                                                                        >  2:1016558/1‑140 (MQ=255)
                   cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCACTACCCa                                                                                                                        >  1:662413/1‑140 (MQ=255)
                   cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCa                                                                                                                        >  2:473963/1‑140 (MQ=255)
                   cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCACTCCCCa                                                                                                                        >  1:1832147/1‑140 (MQ=255)
                       aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                    <  2:550790/140‑1 (MQ=255)
                       aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCACAgg                                                                                                                    <  2:1777477/140‑1 (MQ=255)
                                aCCTAAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGACAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACACTAACCCTATACCCAAAGGGGCgggggg                                                                                                           >  2:1135784/1‑140 (MQ=38)
                                 ccTTCACTATTGATACTAAAAGACCTTAACCGTAGGCCGGATAAGACGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGa                                                                                                          <  1:58583/140‑1 (MQ=38)
                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                          <  2:192330/140‑1 (MQ=255)
                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                          <  1:596514/140‑1 (MQ=255)
                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                          <  1:822399/140‑1 (MQ=255)
                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                          <  1:424575/140‑1 (MQ=255)
                                 ccTTAACAAATGATAATAAAAGACATTAACCCTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGAAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGCGAGGGGa                                                                                                          <  1:856865/140‑1 (MQ=38)
                                    tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCACACCCCAAAGGGGCCAGGGGAccc                                                                                                       >  2:408159/1‑139 (MQ=38)
                                    tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACTCCCCAAAAGGGCGATGGGACCg                                                                                                       >  1:736653/1‑140 (MQ=38)
                                      aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                     <  2:944310/140‑1 (MQ=255)
                                      aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                     <  1:461080/140‑1 (MQ=255)
                                      aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                     <  2:420363/140‑1 (MQ=255)
                                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCAACCCAAAGGGGCGAGGGGACCGTTCAGt                                                                                                 >  2:1830916/1‑140 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTa                                                                                                <  2:255020/140‑1 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                <  1:1339129/140‑1 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                <  2:1167533/140‑1 (MQ=255)
                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATACGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACAAAACCCTAACCCCAAAGGGGCGCGGGGACCGTGAAGTa                                                                                                >  2:505249/1‑140 (MQ=38)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCACACCCCAAAGGGGCGAGGGGACCCGTCAGTaa                                                                                               >  1:1270901/1‑139 (MQ=38)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCAAACCCAAAACCACAACCCCAAAGGGGGAGGGGGACCGtgaaaac                                                                                                >  1:1205276/1‑134 (MQ=25)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACCAAACCCTCAACCCCAAGGGGCGAGGGGACCGTTCAGTAc                                                                                               >  2:440056/1‑140 (MQ=39)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                               >  2:1797334/1‑140 (MQ=255)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTACCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                               >  2:1424659/1‑140 (MQ=255)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCGAAACCAATACCCAAAGGGGCGAGGGGGCCGttaaataa                                                                                               >  1:1016358/1‑134 (MQ=37)
                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTAACCACACCCTAAACCAAAACCCAAAGGGGCGAGGGGGCCCttaaataa                                                                                               >  1:1191925/1‑134 (MQ=37)
                                               aataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAAACCACAACCCCAAGGGGCGAGGGGGCCGGTCAGTACGGa                                                                                            >  1:710924/1‑140 (MQ=37)
                                                ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                           <  1:1007304/140‑1 (MQ=255)
                                                ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                           <  1:362967/140‑1 (MQ=255)
                                                    aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCAAACCCTCTCCCCAAAGGGGCCAGGGGACCGGTCAGTAAGGAATTTa                                                                                       >  2:244239/1‑140 (MQ=39)
                                                         aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGGGAGGGGTCCGTTCAGTACGGAATTTATCTTg                                                                                  >  2:38791/1‑140 (MQ=255)
                                                          ttACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACCTCCCGTACGTTCCCTTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                 <  1:1607156/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCTTCGCATCCGGCGAAGCTGGCGCGTTGAATTTTTCAACCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGCGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                 <  2:353058/140‑1 (MQ=38)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                 <  2:1652221/140‑1 (MQ=255)
                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                 <  2:1463560/140‑1 (MQ=255)
                                                           tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                <  1:1725518/140‑1 (MQ=255)
                                                           tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAACCACAACCCAAAGGGGCGAGGGGACCCTTCACTACGGAATTTAGCATGAc                                                                                >  2:658983/1‑140 (MQ=37)
                                                           tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCTCCCCAAATGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGAc                                                                                >  2:715164/1‑140 (MQ=255)
                                                            aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAACCCACACCCCAAAGGGGCGCGGGGACCGTTCCGTACGGAATTTAGCTTGACg                                                                               >  1:1766672/1‑140 (MQ=38)
                                                             aCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACgg                                                                              <  2:927178/140‑1 (MQ=255)
                                                               cggaGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                            <  1:1069448/137‑1 (MQ=255)
                                                                 tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCCAACCCACACCCCAAAGGGGCGAGGGGCCCCGTCAGTACCGGAAATAGCTTGACCGTGCt                                                                          >  1:1434279/1‑140 (MQ=37)
                                                                 tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCGCACCCCCAAAGGGGGAGGGGGCCGGTAAGTACCGAAATTATCGTGAAGGTGCt                                                                          >  1:279408/1‑140 (MQ=21)
                                                                    gCCGGATAAGATGCGCCAGCCTCGCATCCGGCGATGCTGGCGCGTTGAATTATACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                       <  1:1921449/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGATTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                       <  2:1188807/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                       <  2:282328/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                       <  1:1414261/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                       <  1:1545028/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                       <  1:319453/140‑1 (MQ=255)
                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATATTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                       <  2:128800/140‑1 (MQ=255)
                                                                      cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                     <  2:791515/140‑1 (MQ=255)
                                                                      cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACCTCCCGTCCGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                     <  1:300492/140‑1 (MQ=255)
                                                                        gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTACCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAAATTAGCTTGACCGTGATAACCaac                                                                   >  2:21238/1‑138 (MQ=38)
                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCGCTCCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAATTTAGCTTTGCGGTGCTATACACCa                                                                  >  2:186298/1‑140 (MQ=255)
                                                                               tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                            <  1:670444/140‑1 (MQ=255)
                                                                                 cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                          <  1:800551/140‑1 (MQ=255)
                                                                                  gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                        >  1:130774/1‑140 (MQ=255)
                                                                                   ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                        >  2:1733664/1‑140 (MQ=255)
                                                                                    cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                       <  2:753909/140‑1 (MQ=255)
                                                                                      gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCa                                                     <  1:1512154/140‑1 (MQ=255)
                                                                                         tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                  <  1:1152909/140‑1 (MQ=255)
                                                                                         tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCGCTCCCCCAAGGGGCGAGGGGACCGGTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                  >  2:899514/1‑140 (MQ=255)
                                                                                                  gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAGCCTAACCCTATGCCCAAAGGGGCGATGGGTACGTTCAGTACGGAATTTAGGGTGACGGTGCTATGCAGCATTAAAATGTGGCAATACTCTAACgg                                         >  2:1592315/1‑139 (MQ=255)
                                                                                                     aTGCTGGCGCGTTGAATTTTACGTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                      <  2:824289/140‑1 (MQ=255)
                                                                                                     aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                      <  1:80914/140‑1 (MQ=255)
                                                                                                     aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                      <  1:4683/140‑1 (MQ=255)
                                                                                                                ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                        >  2:316789/1‑47 (MQ=255)
                                                                                                                ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTGACGCAAAATCCGCAGCAc                           <  1:939980/140‑1 (MQ=255)
                                                                                                                  gAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCg                         <  2:381343/140‑1 (MQ=255)
                                                                                                                   aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                        <  1:450144/140‑1 (MQ=255)
                                                                                                                        tACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                   <  2:1440243/140‑1 (MQ=255)
                                                                                                                        tACATCCCGTACGTTAACCACACCCTAACCCTCTCCCCAAACGGGCGAGGGGAACCTTCAGAACCGAAAATAGCTTGACGGTGCAAACCACCATTAAAAAAAGGCAAAAAACTCACGCAAAAACCCCACCACCGtcaaat                   >  2:163103/1‑136 (MQ=255)
                                                                                                                           aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTATCTTGACGGTGCTATCCAGCATTAAATACTGGCTATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                <  1:956892/140‑1 (MQ=255)
                                                                                                                           aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                <  1:1377514/140‑1 (MQ=255)
                                                                                                                               cGTACGTTCCCCACACCCTAACCCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAAAATTGCAATACTCTCACGCAAAATACGCAGCACCGTCTCATAACCGCt            >  2:872466/1‑140 (MQ=37)
                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt       <  1:620898/140‑1 (MQ=255)
                                                                                                                                     ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTGGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt      <  2:761160/140‑1 (MQ=255)
                                                                                                                                       ccccTCACCCTAAACCACTCCCCAAAGGGGCGATGGGACCGTTCACTACGGAATTTATGTTTACGGTGCTATCCACCATTAAATAAAGGCAATAATCTAACGCAAAATCCGCAGCACCCACTCTTCACCCCAGCGAttag    >  2:1523643/1‑138 (MQ=255)
                                                                                                                                        cccTCACCCAAAACCTCACCCCAAATGGGCGATGGGACCGTTAAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATAAAGGCAAAACACTAACGCAAAAAACGCAGCACCGTCACTTAAACGCTGCGATTAgg   >  2:602970/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGG  >  NC_000913/3504722‑3504998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: