Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,635,235 T→G 5.1% L132R (CTG→CGG)  yhiM → acid resistance protein, inner membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,635,2350TG 5.1% 291.4 / 5.0 98L132R (CTG→CGG) yhiMacid resistance protein, inner membrane
Reads supporting (aligned to +/- strand):  ref base T (48/44);  new base G (2/3);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTACG  >  NC_000913/3635098‑3635368
                                                                                                                                         |                                                                                                                                     
cACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGc                                                                                                                                     >  1:1698624/1‑140 (MQ=255)
cACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGACATCCGGGCACTTTATGc                                                                                                                                     >  2:649559/1‑140 (MQ=255)
  ccATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTc                                                                                                                                   >  1:1190091/1‑140 (MQ=255)
    aTGATATTGGGTTGGGCACTGTTAGCAGGCAATTATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCTGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCAt                                                                                                                                 >  2:1663899/1‑140 (MQ=255)
    aTGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCAt                                                                                                                                 >  2:948900/1‑140 (MQ=255)
    aTGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCAGGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGACATCCGGTCACTTTCTGCTCAt                                                                                                                                 >  2:947411/1‑140 (MQ=255)
      gATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTc                                                                                                                               >  1:1550258/1‑140 (MQ=255)
      gATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTc                                                                                                                               >  1:328694/1‑140 (MQ=255)
      gATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCCGCTCATTc                                                                                                                               >  1:707344/1‑140 (MQ=255)
       atatGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTcc                                                                                                                              >  2:489603/1‑140 (MQ=255)
                 gggCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCa                                                                                                                    >  1:927812/1‑140 (MQ=255)
                 gggCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCa                                                                                                                    >  1:1716852/1‑140 (MQ=255)
                 gggCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCAGGTCACTTTCTGCTCATTCCCAAAAATGCa                                                                                                                    >  1:1333231/1‑140 (MQ=255)
                  ggCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAg                                                                                                                   >  2:1003199/1‑140 (MQ=255)
                  ggCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAg                                                                                                                   >  1:866127/1‑140 (MQ=255)
                  ggCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAg                                                                                                                   >  2:27663/1‑140 (MQ=255)
                  ggCACTGTTAGCAGGCAATGATGTGATGGCAGACCAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAg                                                                                                                   >  2:1596181/1‑140 (MQ=255)
                    cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTTTTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCg                                                                                                                 <  1:769816/140‑1 (MQ=255)
                    cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCg                                                                                                                 <  1:234854/140‑1 (MQ=255)
                    cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCg                                                                                                                 <  2:1685254/140‑1 (MQ=255)
                    cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCg                                                                                                                 <  2:651274/140‑1 (MQ=255)
                    cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCg                                                                                                                 <  1:769625/140‑1 (MQ=255)
                    cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCg                                                                                                                 <  1:1064764/140‑1 (MQ=255)
                    cACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCg                                                                                                                 <  1:1688401/140‑1 (MQ=255)
                       tGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCgggg                                                                                                              >  1:361947/1‑140 (MQ=255)
                       tGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCgggg                                                                                                              >  1:1387490/1‑140 (MQ=255)
                       tGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCgggg                                                                                                              >  1:41178/1‑140 (MQ=255)
                       tGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCgggg                                                                                                              >  1:395208/1‑140 (MQ=255)
                       tGTTAGCAGGCAATGATGTGATGGCAGACGAGGTTGTCGCCGGACATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCgggg                                                                                                              >  2:725942/1‑140 (MQ=255)
                         ttAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCGGCTCATTCCCAAAAATGCAGCGGGGAg                                                                                                            >  1:251112/1‑140 (MQ=255)
                         ttAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCGACTCATTCCCAAAAATGCAGCGGGGAg                                                                                                            >  1:251110/1‑140 (MQ=255)
                          tAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGc                                                                                                           <  2:720272/140‑1 (MQ=255)
                          tAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGc                                                                                                           <  2:533712/140‑1 (MQ=255)
                          tAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGc                                                                                                           <  2:536675/140‑1 (MQ=255)
                                 cAATGATGTGATGGCAGACGAGTTTGTCGCCGGCGATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATACGGTCACTTTCTGCTCATTCACAAATATGCAGCGGGGTGCTAGAGCg                                                                                                    >  1:1481011/1‑140 (MQ=255)
                                 cAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCg                                                                                                    >  1:218312/1‑140 (MQ=255)
                                 cAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCg                                                                                                    >  1:219443/1‑140 (MQ=255)
                                   atgatgTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGAc                                                                                                  >  1:1287255/1‑140 (MQ=255)
                                   atgatgTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGAc                                                                                                  >  1:1194568/1‑140 (MQ=255)
                                   atgatgTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGAc                                                                                                  >  1:1723685/1‑140 (MQ=255)
                                     gatgtgatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACgg                                                                                                <  1:577760/140‑1 (MQ=255)
                                          gatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAacac                                                                                           <  2:1409140/140‑1 (MQ=255)
                                          gatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAacac                                                                                           <  1:567097/140‑1 (MQ=255)
                                          gatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAacac                                                                                           <  1:572114/140‑1 (MQ=255)
                                          gatgGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAacac                                                                                           <  1:1429701/140‑1 (MQ=255)
                                               cAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTa                                                                                      <  1:1750948/140‑1 (MQ=255)
                                               cAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTa                                                                                      <  1:1442756/140‑1 (MQ=255)
                                               cAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTa                                                                                      <  1:1671120/140‑1 (MQ=255)
                                               cAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCGGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTa                                                                                      <  2:1878114/140‑1 (MQ=255)
                                                aGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTAc                                                                                     >  1:817399/1‑140 (MQ=255)
                                                aGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTAc                                                                                     >  1:1680103/1‑140 (MQ=255)
                                                  aCGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAaca                                                                                            >  1:317133/1‑131 (MQ=255)
                                                  aCGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAaca                                                                                            <  2:317133/131‑1 (MQ=255)
                                                            cGCCGGCCTTGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGGGGCTGCGTCATCCGGTCACTGTCTGCTCAGTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTTttctt                                                                         <  2:1073432/140‑1 (MQ=255)
                                                               cGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCAt                                                                      <  1:961558/140‑1 (MQ=255)
                                                                   cATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAAt                                                                  <  2:249764/140‑1 (MQ=255)
                                                                   cATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAAt                                                                  <  2:250156/140‑1 (MQ=255)
                                                                   cATGTTATGTTCTGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAAt                                                                  <  2:283577/140‑1 (MQ=255)
                                                                           tttCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACt                                                          >  1:634444/1‑140 (MQ=255)
                                                                              cGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGcc                                                       >  1:1562399/1‑140 (MQ=255)
                                                                                 cGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCa                                                    >  1:1480555/1‑140 (MQ=255)
                                                                                  gTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCAt                                                   <  2:1369853/140‑1 (MQ=255)
                                                                                              gccgccTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGt                                       <  2:1331443/140‑1 (MQ=255)
                                                                                              gccgccTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGt                                       <  1:66975/140‑1 (MQ=255)
                                                                                              gccgccTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGt                                       <  2:290081/140‑1 (MQ=255)
                                                                                                gacaggtgtATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGttt                                     >  1:123253/6‑140 (MQ=255)
                                                                                                 gccTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGtttt                                    >  2:588352/1‑140 (MQ=255)
                                                                                                             aCGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGTGCGACGGAACACCGGTACAGGGTTATTCTTCATTATTCGGTAACTGCCTCATTGCCGTTCCCGGTTTACTCTCCCGGcg                        >  1:1922302/1‑139 (MQ=255)
                                                                                                             aCGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCt                        >  2:989333/1‑140 (MQ=255)
                                                                                                             aCGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCt                        >  2:993306/1‑140 (MQ=255)
                                                                                                             aCGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCt                        >  1:729661/1‑140 (MQ=255)
                                                                                                             aCGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCt                        >  1:736677/1‑140 (MQ=255)
                                                                                                             aCGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCt                        >  2:1066464/1‑140 (MQ=255)
                                                                                                                gTGGCAGCGTCATCCGGTCACTTTCTGCTCGTTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCgg                     >  1:1783564/1‑140 (MQ=255)
                                                                                                                gTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCgg                     >  1:1506676/1‑140 (MQ=255)
                                                                                                                gTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGCAACACCGGTACAGGCTTATTCTTCATTAATCGGAAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCgg                     >  2:870657/1‑140 (MQ=255)
                                                                                                                      gCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCAt               <  2:1600700/140‑1 (MQ=255)
                                                                                                                       cGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCAtt              <  2:361947/140‑1 (MQ=255)
                                                                                                                         tCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTg            <  2:41178/140‑1 (MQ=255)
                                                                                                                         tCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTg            <  2:952315/140‑1 (MQ=255)
                                                                                                                         tCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTg            <  2:927812/140‑1 (MQ=255)
                                                                                                                         tCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTg            <  1:272782/140‑1 (MQ=255)
                                                                                                                           aTCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGt          <  2:714712/140‑1 (MQ=255)
                                                                                                                              cGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTa       <  1:588352/140‑1 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCGTGCTCGGTTTCATTTGGTCTATTa    <  2:1410406/140‑1 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    <  1:40064/140‑1 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    >  1:1000934/1‑140 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    >  1:1010809/1‑140 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    <  2:1410787/140‑1 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    >  1:997301/1‑140 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    <  2:458197/140‑1 (MQ=255)
                                                                                                                                 tCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    <  2:1364714/140‑1 (MQ=255)
                                                                                                                                 tCACTTTCGGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    <  2:251112/140‑1 (MQ=255)
                                                                                                                                 tCACTTTCGACTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTa    <  2:251110/140‑1 (MQ=255)
                                                                                                                                   aCTTTCTGCTCATTCCCATAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATACTTCATTAATCGGTAACGGCCTCAGTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTACg  <  2:1321944/140‑1 (MQ=255)
                                                                                                                                   aCTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTACg  <  1:595862/140‑1 (MQ=255)
                                                                                                                                     tttCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACgg                                                                                                >  1:1696188/1‑44 (MQ=255)
                                                                                                                                     tttCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACgg                                                                                                <  2:1696188/44‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CACCATGATATGGGGTTGGGCACTGTTAGCAGGCAATGATGTGATGGCAGACGAGTTTGTCGCCGGCCATGTTATTTTCGGCGTTGGTATGATTGCCGCCTGTGTATCGACGGTGGCAGCGTCATCCGGTCACTTTCTGCTCATTCCCAAAAATGCAGCGGGGAGCAAGAGCGACGGAACACCGGTACAGGCTTATTCTTCATTAATCGGTAACTGCCTCATTGCCGTTCCCGTTTTACTCACCCTGCTCGGTTTCATTTGGTCTATTACG  >  NC_000913/3635098‑3635368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: