Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,907,574 T→C 6.9% D2G (GAC→GGC)  phoU ← negative regulator of PhoR/PhoB two‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,907,5740TC 6.9% 373.3 / 14.7 116D2G (GAC→GGC) phoUnegative regulator of PhoR/PhoB two‑component regulator
Reads supporting (aligned to +/- strand):  ref base T (60/47);  new base C (5/3);  total (66/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 119 total aligned reads displayed.

TGTCCTGGTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAA  >  NC_000913/3907437‑3907713
                                                                                                                                         |                                                                                                                                           
tGTCCTGGTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTcc                                                                                                                                           >  2:4415/1‑140 (MQ=255)
 gTCCTGGTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCa                                                                                                                                          <  2:1153374/140‑1 (MQ=255)
  tCCTGGTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCAt                                                                                                                                         >  1:1306323/1‑140 (MQ=255)
      ggTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACg                                                                                                                                     <  1:97928/140‑1 (MQ=255)
      ggTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACg                                                                                                                                     <  1:417809/140‑1 (MQ=255)
      ggTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACg                                                                                                                                     <  1:1397938/140‑1 (MQ=255)
       gTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGc                                                                                                                                    >  2:1696114/1‑140 (MQ=255)
       gTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGCCCATAACGc                                                                                                                                    >  2:1107515/1‑140 (MQ=255)
       gTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGCCCATAACGc                                                                                                                                    >  1:1359731/1‑140 (MQ=255)
       gTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGCCCATAACGc                                                                                                                                    >  2:292895/1‑140 (MQ=255)
        ttATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGAATGTCCATAACGCa                                                                                                                                   <  1:1742384/140‑1 (MQ=255)
            gCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAACTATGTTTATTAAGATTGAGACTGTCCATAACGCACTcc                                                                                                                               >  1:1806889/1‑140 (MQ=255)
            gCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTcc                                                                                                                               >  1:1679918/1‑140 (MQ=255)
              aTCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTg                                                                                                                             >  1:1457566/1‑140 (MQ=255)
                 gcgGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAAt                                                                                                                          >  1:780517/1‑140 (MQ=255)
                 gcgGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTAATTAAGATTGAGACTGTCCATAACGCACTCCTGAAt                                                                                                                          >  1:1211722/1‑140 (MQ=255)
                         tGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTa                                                                                                                  <  2:74957/140‑1 (MQ=255)
                              cAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGAc                                                                                                             <  1:749286/140‑1 (MQ=255)
                              cAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGCCCATAACGCACTCCTGAATCAACCGTAACGAc                                                                                                             <  1:1337033/140‑1 (MQ=255)
                                   aGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTg                                                                                                        <  2:259779/140‑1 (MQ=255)
                                   aGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTg                                                                                                        >  1:269084/1‑140 (MQ=255)
                                     ctgctgctCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGAt                                                                                                      <  2:1734115/140‑1 (MQ=255)
                                     ctgctgctCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGAt                                                                                                      <  2:1733179/140‑1 (MQ=255)
                                      tgctgctCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATg                                                                                                     <  1:1279042/140‑1 (MQ=255)
                                      tgctgctCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATg                                                                                                     >  2:1380409/1‑140 (MQ=255)
                                      tgctgctCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATg                                                                                                     >  2:1379994/1‑140 (MQ=255)
                                           ctCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAGCTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGtc                                                                                                >  1:920738/1‑140 (MQ=255)
                                           ctCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGtc                                                                                                >  1:1623115/1‑140 (MQ=255)
                                                ccaTGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCtg                                                                                           >  1:1569929/1‑140 (MQ=255)
                                                ccaTGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCtg                                                                                           >  1:1569923/1‑140 (MQ=255)
                                                     ccgccCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCtgtttgt                                                                                      >  1:1297658/1‑140 (MQ=255)
                                                        cccATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTttc                                                                                   >  2:1375383/1‑140 (MQ=255)
                                                          cATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTttctt                                                                                 >  1:226227/1‑140 (MQ=255)
                                                             ggTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGc                                                                              >  1:1789730/1‑140 (MQ=255)
                                                                 atcaCCTGCGTGCGGATACTTTCCAGTTCGGCGTTGATCTGGCCGGAAATATGTTTATTAAGATTGAGACTGCCCATAACGCACTCCTGAATCTAACTTAAAGACCGTTGTTGTAGTCTTCTGttttttttttgggtgta                                                                          <  1:1757801/140‑8 (MQ=255)
                                                                  tcaCCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCt                                                                         <  2:1810854/140‑1 (MQ=255)
                                                                   caCCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCtt                                                                        >  2:405400/1‑140 (MQ=255)
                                                                       tgcgtgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTg                                                                    <  1:1832696/140‑1 (MQ=255)
                                                                        gcgtgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGa                                                                   >  1:420780/1‑140 (MQ=255)
                                                                        gcgtgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGa                                                                   >  1:421007/1‑140 (MQ=255)
                                                                        gcgtgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGa                                                                   >  1:1102483/1‑140 (MQ=255)
                                                                         cgtgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTCGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGac                                                                  >  1:443263/1‑139 (MQ=255)
                                                                         cgtgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGaa                                                                  >  1:445357/1‑140 (MQ=255)
                                                                         cgtgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGCCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGaa                                                                  <  2:1359731/140‑1 (MQ=255)
                                                                         cgtgcgGATACTTTCCAGATCGGCGTTGAACTGGCCGGAAATATGGTTAATAAGATAGAGACTGACCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCAGGCTTGGTGaa                                                                  >  2:1076878/1‑140 (MQ=255)
                                                                           tgcgGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACa                                                                <  1:477401/140‑1 (MQ=255)
                                                                                      tCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCgtg                                                     >  2:637663/1‑140 (MQ=255)
                                                                                      tCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCgtg                                                     >  2:1047558/1‑140 (MQ=255)
                                                                                         aGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTg                                                  >  1:545834/1‑140 (MQ=255)
                                                                                          gTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGc                                                 >  1:700532/1‑140 (MQ=255)
                                                                                          gTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGc                                                 >  2:1209627/1‑140 (MQ=255)
                                                                                              ggCGTTGAACTGGCCGGAAATATGGTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGaa                                             >  1:376452/1‑140 (MQ=255)
                                                                                               ccGTTGCACAGGACGGAAACATGAATAAGCAGATTTAGACAGTCCAAAACCCACACCAGAATCAACCGTAACCACCGGTGATGTAGACTTCCGCTCGTTTCGTCGCCGGCTTGGTGAACAGCTCGTCCGTGTTGCTGAAc                                            <  2:1922597/139‑1 (MQ=255)
                                                                                                   tGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACtcaa                                        <  2:277074/140‑1 (MQ=255)
                                                                                                     aaCTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACtcaatc                                      >  2:780004/1‑140 (MQ=255)
                                                                                                          gCCGGAAATATGTTTATTAAGATTGAGTCTGTCCATAACGCACTCCTGAAACAACCGTCACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTACGTGTTGCTGGACTcgatcaatgc                                 >  2:31308/1‑138 (MQ=255)
                                                                                                          gCCGGAAATATGTTTATTAAGATTGAGACTGCCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTc                                 >  1:710862/1‑140 (MQ=255)
                                                                                                           ccGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCg                                <  2:1341843/140‑1 (MQ=255)
                                                                                                            cGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGc                               <  1:1638011/140‑1 (MQ=255)
                                                                                                             ggAAATCTGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTACTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGcc                              <  1:735924/140‑1 (MQ=255)
                                                                                                               aaaTATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCa                            <  2:593890/140‑1 (MQ=255)
                                                                                                               aaaTATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCa                            <  2:1669233/140‑1 (MQ=255)
                                                                                                                 atatGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAgg                          <  2:16687/140‑1 (MQ=255)
                                                                                                                 atatGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAgg                          <  2:1306225/140‑1 (MQ=255)
                                                                                                                 atatGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAgg                          <  2:1679918/140‑1 (MQ=255)
                                                                                                                 atatGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAgg                          <  2:1477851/140‑1 (MQ=255)
                                                                                                                 atatGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAgg                          <  2:581926/140‑1 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:208984/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:768697/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  1:166270/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:401547/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:401413/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:23484/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:84271/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:208916/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:208494/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:1225135/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:1225220/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  1:239992/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  2:207862/1‑140 (MQ=255)
                                                                                                                    tGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTAc                       >  1:1804164/1‑140 (MQ=255)
                                                                                                                     gTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACa                      <  2:239992/140‑1 (MQ=255)
                                                                                                                     gTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACa                      <  1:1739898/140‑1 (MQ=255)
                                                                                                                     gTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACa                      <  2:551053/140‑1 (MQ=255)
                                                                                                                     gTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACa                      <  1:1250786/140‑1 (MQ=255)
                                                                                                                       ttattaAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATa                    <  1:1747310/140‑1 (MQ=255)
                                                                                                                         attaAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATaaa                  >  1:307039/1‑140 (MQ=255)
                                                                                                                             aGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGcc              <  1:185403/140‑1 (MQ=255)
                                                                                                                             aGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTATTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGcc              <  1:185193/140‑1 (MQ=255)
                                                                                                                               aTTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCgt            <  2:786895/140‑1 (MQ=255)
                                                                                                                                ttGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCgtg           >  1:629269/1‑140 (MQ=255)
                                                                                                                                ttGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCgtg           >  1:303693/1‑140 (MQ=255)
                                                                                                                                    gaCTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGt       <  1:142883/140‑1 (MQ=255)
                                                                                                                                    gaCTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTATTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGt       <  1:369363/140‑1 (MQ=255)
                                                                                                                                     aCTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTc      >  2:853594/1‑140 (MQ=255)
                                                                                                                                       tGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCgg    >  2:1467356/1‑140 (MQ=255)
                                                                                                                                       tGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCgg    >  2:284021/1‑140 (MQ=255)
                                                                                                                                         tCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGaa  >  2:141220/1‑140 (MQ=255)
                                                                                                                                         tCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGaa  >  1:567983/1‑140 (MQ=255)
                                                                                                                                         tCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGGAGTCTTCTGTTTGTTTCTTCGCTGGCGTGGTGAACAGATCGTCCGTGTGGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGaa  >  2:1716228/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGTCCTGGTTATGCATCGCGGTGATTGCATCAGAAAGCTGCTGCTCCACCATGCCGCCCATGGTCATCACCTGCGTGCGGATACTTTCCAGTTCGGCGTTGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAA  >  NC_000913/3907437‑3907713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: