Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,936,327 C→T 7.1% A17V (GCC→GTC)  rbsB → D‑ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,936,3270CT 7.1% 285.3 / 9.3 85A17V (GCC→GTC) rbsBD‑ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (34/45);  new base T (3/3);  total (37/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

ACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGA  >  NC_000913/3936189‑3936465
                                                                                                                                          |                                                                                                                                          
aCCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGcc                                                                                                                                           >  1:1736785/1‑140 (MQ=255)
     atgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGt                                                                                                                                      <  2:91415/140‑1 (MQ=255)
     atgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGt                                                                                                                                      >  2:892351/1‑140 (MQ=255)
     atgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGt                                                                                                                                      >  1:1482572/1‑140 (MQ=255)
     atgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACAGCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTGTCCGCTGTTGCGCTAAGCGCCACCGt                                                                                                                                      >  2:1815793/1‑140 (MQ=255)
     atgatCGTCAAAGCGGTGGTGATTTTGCCGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAATACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGt                                                                                                                                      >  1:1468248/1‑140 (MQ=255)
     atgatCGGCAAACCGGTGGTGATATTGCTGGCGGTGCTGGTAGACCCCAAAAACCAGAAATCACGACTACAGGACATCATGAATATTAACATGAAAAAACTGCCTACCCTGGTTAACGCTGTTGCGCAAAGAGCCACCgg                                                                                                                                      >  2:361020/1‑139 (MQ=255)
      tgatCTTCAAAGCTTTGTTGTTTTTGCAGGCGGTGCTGGTAGACAAAAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGACCTTGAAAAATATGTCTACCCTGGTTTCCGCTTTTTCGCTAAGCGCCACCGta                                                                                                                                     <  2:895278/140‑2 (MQ=255)
      tgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTc                                                                                                                                     <  2:1208058/140‑1 (MQ=255)
      tgatCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTc                                                                                                                                     <  2:1837108/140‑1 (MQ=255)
                  cGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCg                                                                                                                         >  1:1621436/1‑140 (MQ=255)
                  cGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCg                                                                                                                         >  1:1621170/1‑140 (MQ=255)
                   ggtggtGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGa                                                                                                                        <  2:923515/140‑1 (MQ=255)
                     tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAATGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                                                      >  2:1090316/1‑140 (MQ=255)
                     tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                                                      <  2:1853180/140‑1 (MQ=255)
                     tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                                                      <  2:1853325/140‑1 (MQ=255)
                     tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                                                      <  2:780326/140‑1 (MQ=255)
                     tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                                                      >  2:1092629/1‑140 (MQ=255)
                     tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                                                      >  2:1090293/1‑140 (MQ=255)
                     tggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATg                                                                                                                      >  1:1829219/1‑140 (MQ=255)
                     tggtgATTTTGCGGTCGGTGCTTGTAGACAACAAAATGCTGAAATAACGACTCCCGTACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGGTGTTGCGCTTAGCGCCTCCGTCAGTGCGAATGCGATg                                                                                                                      <  2:1295980/140‑1 (MQ=255)
                      ggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATgg                                                                                                                     <  2:167586/140‑1 (MQ=255)
                      ggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATgg                                                                                                                     <  2:1158019/140‑1 (MQ=255)
                      ggtgATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATgg                                                                                                                     <  2:636460/140‑1 (MQ=255)
                        cgATTTTGCTGGCGGAGCTGGTAGCCAACAACAAGCAGTAAGAACGACTACAGCCCATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCa                                                                                                                   <  1:305927/139‑1 (MQ=255)
                             ttGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGa                                                                                                              <  1:412120/140‑1 (MQ=255)
                                   gCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCAt                                                                                                        <  1:209562/140‑1 (MQ=255)
                                   gCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCAt                                                                                                        <  1:209652/140‑1 (MQ=255)
                                       tGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAACAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATcgcg                                                                                                    <  1:488878/140‑1 (MQ=255)
                                       tGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATcgcg                                                                                                    <  1:1188217/140‑1 (MQ=255)
                                       tGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATcgcg                                                                                                    <  1:1207341/140‑1 (MQ=255)
                                       tGCTGGTAGACAACAAAAAGCAGTAAGAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATcgcg                                                                                                    <  1:1209431/140‑1 (MQ=255)
                                       agCTGGTTCACAACAAAACGCATTTATCCCGAGCAGAGGACATCTTGAATATGAACATGCCAACACTGGCTACCCTGGTTACCGCTGTTGCTCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGCCACCCTcgcg                                                                                                    <  2:1560030/139‑1 (MQ=255)
                                                     aaaaaGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCAc                                                                                      <  2:549402/140‑1 (MQ=255)
                                                              taataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGTCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTaacaa                                                                             >  2:183586/1‑140 (MQ=255)
                                                              taataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGTCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTaacaa                                                                             >  2:183744/1‑140 (MQ=255)
                                                              taataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGTCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTaacaa                                                                             >  2:192957/1‑140 (MQ=255)
                                                              taataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTaacaa                                                                             >  1:520090/1‑140 (MQ=255)
                                                               aataaCGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTaacaac                                                                            <  2:1794483/140‑1 (MQ=255)
                                                                     gACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTc                                                                      >  1:242873/1‑140 (MQ=255)
                                                                      aCTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCt                                                                     >  2:1385945/1‑140 (MQ=255)
                                                                      aCTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCt                                                                     >  2:1383659/1‑140 (MQ=255)
                                                                       cTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCtt                                                                    >  1:388705/1‑140 (MQ=255)
                                                                        tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCTCTTTTGCGCTAAGCGCCACCGTCAGTGCGATTTGGATGGCAAAATACAACATCTCGATGGTGGAATCCACGCATAACAACCCGTTCttt                                                                   >  2:1279783/1‑140 (MQ=255)
                                                                            ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTAt                                                               <  1:879197/140‑1 (MQ=255)
                                                                               cATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGc                                                            >  2:906489/1‑140 (MQ=255)
                                                                                  cTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGa                                                         >  1:162576/1‑140 (MQ=255)
                                                                                  cTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGa                                                         >  1:1419339/1‑140 (MQ=255)
                                                                                  cTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGa                                                         >  1:1419487/1‑140 (MQ=255)
                                                                                     gAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                                      >  1:48409/1‑140 (MQ=255)
                                                                                     gAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                                      <  1:344694/140‑1 (MQ=255)
                                                                                     gAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                                      >  1:907445/1‑140 (MQ=255)
                                                                                     gAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAg                                                      >  1:48662/1‑140 (MQ=255)
                                                                                           gAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcg                                                <  2:1149406/140‑1 (MQ=255)
                                                                                            aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACACCCCGTTCTGTGTATCGCTGAAAGATGgcgc                                               >  2:1824865/1‑140 (MQ=255)
                                                                                            aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGTAAGATGgcgc                                               >  1:985452/1‑140 (MQ=255)
                                                                                            aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgc                                               >  2:1676774/1‑140 (MQ=255)
                                                                                            aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTACACAACCCGTTCTGTGTATCGCGGAAAGATGgcgc                                               >  2:1824495/1‑140 (MQ=255)
                                                                                             aCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                              <  2:1511086/140‑1 (MQ=255)
                                                                                                     aaaCTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAaga                                      <  2:867858/140‑1 (MQ=255)
                                                                                                          ggCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCgg                                 >  1:102849/1‑140 (MQ=255)
                                                                                                           gCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTATCAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGa                                >  2:6582/1‑140 (MQ=255)
                                                                                                                ccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGCTGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAc                           <  2:529855/140‑1 (MQ=255)
                                                                                                                ccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAc                           <  2:529936/140‑1 (MQ=255)
                                                                                                                ccTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCACGCAGAAAGAGGCGGATAAAc                           <  2:1759630/140‑1 (MQ=255)
                                                                                                                 cTGGTTTCCGCTGTTGCGCTAAGCGTCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                          <  1:192957/140‑1 (MQ=255)
                                                                                                                 cTGGTTTCCGCTGTTGCGCTAAGCGTCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                          <  1:183586/140‑1 (MQ=255)
                                                                                                                 cTGGTTTCCGCTGTTGCGCTAAGCGTCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                          <  1:183744/140‑1 (MQ=255)
                                                                                                                 cTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                          <  2:1823318/140‑1 (MQ=255)
                                                                                                                 cTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                          <  2:433385/140‑1 (MQ=255)
                                                                                                                 cTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                          <  2:388705/140‑1 (MQ=255)
                                                                                                                  tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACtt                         <  2:1491485/140‑1 (MQ=255)
                                                                                                                     tttCCACTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGc                      <  2:553265/140‑1 (MQ=255)
                                                                                                                       tCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTTGTGGTCTCCACGCTTAACAACCCGTTATTTGTATCGCTGAAAGATGGAGCGCAGAAAGAGGCGGATTAACTTGGCta                    <  1:980911/140‑1 (MQ=255)
                                                                                                                       tCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCta                    <  1:1467009/140‑1 (MQ=255)
                                                                                                                       tCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCta                    <  1:1466806/140‑1 (MQ=255)
                                                                                                                       tCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCta                    <  1:1467071/140‑1 (MQ=255)
                                                                                                                            tGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAAcc               <  1:281183/140‑1 (MQ=255)
                                                                                                                            tGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAAcc               <  1:307235/140‑1 (MQ=255)
                                                                                                                             gTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                              >  1:1829794/1‑108 (MQ=255)
                                                                                                                             gTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                              <  2:1829794/108‑1 (MQ=255)
                                                                                                                              ttGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtg             >  1:478879/1‑140 (MQ=255)
                                                                                                                              ttGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtg             >  1:332251/1‑140 (MQ=255)
                                                                                                                               tGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg            <  2:820399/140‑1 (MQ=255)
                                                                                                                               tGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg            <  2:819104/140‑1 (MQ=255)
                                                                                                                                   cTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCGTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACGTGGCTATAACCTggtggt        >  2:1802525/1‑140 (MQ=255)
                                                                                                                                        cgcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg   <  1:892351/140‑1 (MQ=255)
                                                                                                                                        cgcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg   <  2:1117055/140‑1 (MQ=255)
                                                                                                                                        cgcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg   <  1:230046/140‑1 (MQ=255)
                                                                                                                                        cgcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg   <  2:1780815/140‑1 (MQ=255)
                                                                                                                                        cgcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGAGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg   >  1:680468/1‑140 (MQ=255)
                                                                                                                                         gcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGa  <  1:204068/140‑1 (MQ=255)
                                                                                                                                         gcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGa  <  1:1056495/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACCAGATGATCGTCAAAGCGGTGGTGATTTTGCTGGCGGTGCTGGTAGACAACAAAAAGCAGTAATAACGACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGA  >  NC_000913/3936189‑3936465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: