Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,213,390 C→T 5.6% Q53* (CAA→TAA)  yjaA → stress‑induced protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,213,3900CT 5.6% 249.3 / 3.9 71Q53* (CAA→TAA) yjaAstress‑induced protein
Reads supporting (aligned to +/- strand):  ref base C (23/44);  new base T (2/2);  total (25/46)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGC  >  NC_000913/4213251‑4213526
                                                                                                                                           |                                                                                                                                        
ccAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGc                                                                                                                                          >  2:982095/1‑140 (MQ=255)
ccAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGc                                                                                                                                          >  1:762092/1‑140 (MQ=255)
ccAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGc                                                                                                                                          >  1:440544/1‑140 (MQ=255)
  aaaTTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCaa                                                                                                                                        >  1:1386848/1‑140 (MQ=255)
  aaaTTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCaa                                                                                                                                        >  1:1017350/1‑140 (MQ=255)
  aaaTTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCaa                                                                                                                                        >  1:1900387/1‑140 (MQ=255)
     ttCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTc                                                                                                                                     >  2:1472347/1‑140 (MQ=255)
     ttCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTc                                                                                                                                     >  2:141187/1‑140 (MQ=255)
       cgtcgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTcca                                                                                                                                   >  2:1375393/1‑140 (MQ=255)
         tcgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccacc                                                                                                                                 <  2:1853984/140‑1 (MQ=255)
         tcgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccacc                                                                                                                                 <  2:417959/140‑1 (MQ=255)
         tcgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccacc                                                                                                                                 <  2:1355067/140‑1 (MQ=255)
         tcgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccacc                                                                                                                                 <  2:1284897/140‑1 (MQ=255)
         tcgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccacc                                                                                                                                 <  2:1284815/140‑1 (MQ=255)
         tcgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccacc                                                                                                                                 <  1:1396371/140‑1 (MQ=255)
         tcgtAAGCAAATTACTGTTCGCGATCTTGAACGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATGTCTTAGCGGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccacc                                                                                                                                 <  2:889560/140‑1 (MQ=255)
          cgtAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTccaccc                                                                                                                                <  2:420067/140‑1 (MQ=255)
               tCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGtt                                                                                                                           <  1:259396/140‑1 (MQ=255)
                         gTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCa                                                                                                                 >  2:1807840/1‑140 (MQ=255)
                         gTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCa                                                                                                                 >  2:1808019/1‑140 (MQ=255)
                          ttCGCGATCTTGTAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCTAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCAt                                                                                                                <  2:950072/140‑1 (MQ=255)
                          ttCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCAt                                                                                                                >  2:1802833/1‑140 (MQ=255)
                          ttCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCAt                                                                                                                <  1:220969/140‑1 (MQ=255)
                          ttCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCAt                                                                                                                <  1:221926/140‑1 (MQ=255)
                          ttCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCAt                                                                                                                <  2:1248902/140‑1 (MQ=255)
                                    tGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGc                                                                                                      >  1:1771197/1‑140 (MQ=255)
                                       aaGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTATGCAAGATCTTGTTCTGTAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAg                                                                                                   >  1:593612/1‑140 (MQ=255)
                                       aaGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTATGCAAGATCTTGTTCTGTAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAg                                                                                                   >  1:593845/1‑140 (MQ=255)
                                          cAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCaa                                                                                                <  1:312465/140‑1 (MQ=255)
                                          cAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCaa                                                                                                <  2:1823440/140‑1 (MQ=255)
                                           aaaCGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCaaa                                                                                               <  2:45221/140‑1 (MQ=255)
                                               gTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGt                                                                                           <  1:461904/140‑1 (MQ=255)
                                                 gAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGACAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTAt                                                                                         <  2:1479134/140‑1 (MQ=255)
                                                 gAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTAt                                                                                         <  1:70680/140‑1 (MQ=255)
                                                 gAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTAt                                                                                         <  1:982095/140‑1 (MQ=255)
                                                   aGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATgg                                                                                       <  2:1738563/140‑1 (MQ=255)
                                                      gtCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGata                                                                                    <  1:1584690/140‑1 (MQ=255)
                                                      gtCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGata                                                                                    <  2:690427/140‑1 (MQ=255)
                                                       tCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGatat                                                                                   >  2:1507993/1‑140 (MQ=255)
                                                                 cTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAgc                                                                         >  1:1613582/1‑140 (MQ=255)
                                                                  agCCTGCAGTAACCCGCGCCTGGTACACCCCAATTTCTTAGTTGCAGCAAAAGTTCTGCAAGATCTTGCTCAGCAACTCCACCCACGTTCAACCTGGCATTCTATATTGCCAGCAAAACCTATGGATATTGTTGTGAgcg                                                                        <  2:603148/139‑1 (MQ=255)
                                                                    ccTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCa                                                                      >  1:1206010/1‑140 (MQ=255)
                                                                    ccTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCa                                                                      >  1:663230/1‑140 (MQ=255)
                                                                     cTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCAc                                                                     >  1:220422/1‑140 (MQ=255)
                                                                     cTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCAc                                                                     >  1:220402/1‑140 (MQ=255)
                                                                      ttCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACt                                                                    <  1:386425/140‑1 (MQ=255)
                                                                              ccAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATCGTTGTGAGCGCACTGGAaatga                                                            >  2:1007552/1‑140 (MQ=255)
                                                                              ccAGCGCCTGTTAATCGCCAATTTCTTTGTAGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTACAACCTGGCATTCTTTATTGCCAGCAAAACGTATGGATATTGTTGTCAGCGCACTGGAaatga                                                            >  2:1007255/1‑140 (MQ=255)
                                                                              ccAGCGCCTGTTAATCGCAAATTTCTTTGTTGATGAAAAAGTTCTCCAAGTGCTTGTTCTGCAAATCCACCCTCGTTCAACCTGGCATTCTTTTTTGCCTGCATTACGTCTGGATATTGTTGTGAGAGCACTGGAaatga                                                            >  2:1576921/1‑140 (MQ=255)
                                                                                  cgcCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAatgaatga                                                        >  1:121846/1‑140 (MQ=255)
                                                                                     cTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAggg                                                     <  1:1382402/140‑1 (MQ=255)
                                                                                     cTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAggg                                                     <  1:1917708/140‑1 (MQ=255)
                                                                                         tAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCCCGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGt                                                 <  2:92302/140‑1 (MQ=255)
                                                                                         tAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGt                                                 <  2:92284/140‑1 (MQ=255)
                                                                                              gCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTc                                            <  1:141187/140‑1 (MQ=255)
                                                                                              gCCAATTTCATTGTTGCAGAAAAAGTTCTGCAAGATCTTGTGCTGCAACTCCCCCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCTCTGGAAATGAATGAGGGCGGTTTGTc                                            <  2:106100/140‑1 (MQ=255)
                                                                                                cAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTcac                                          <  2:209454/140‑1 (MQ=255)
                                                                                                cAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTcac                                          <  2:908335/140‑1 (MQ=255)
                                                                                                       tttGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGa                                   <  1:548123/140‑1 (MQ=255)
                                                                                                         tgttgCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTAt                                                                                         <  1:671458/84‑1 (MQ=255)
                                                                                                         tgttgCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTAt                                                                                         >  2:671458/1‑84 (MQ=255)
                                                                                                                gAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCAt                          <  1:1507993/140‑1 (MQ=255)
                                                                                                                gAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCAt                          <  1:569453/140‑1 (MQ=255)
                                                                                                                gAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCAt                          <  2:781536/140‑1 (MQ=255)
                                                                                                                gAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCAGTTTGTCACAGGTTGAGGAACGCAt                          <  2:909640/140‑1 (MQ=255)
                                                                                                                 aaaaaGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCAtt                         <  1:1080950/140‑1 (MQ=255)
                                                                                                                 aaaaaGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCAtt                         <  1:1081002/140‑1 (MQ=255)
                                                                                                                     aGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTcc                     <  2:440544/140‑1 (MQ=255)
                                                                                                                               gATCTTGTTCTGTAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGt           <  2:593845/140‑1 (MQ=255)
                                                                                                                               gATCTTGTTCTGTAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGt           <  2:593612/140‑1 (MQ=255)
                                                                                                                                 tCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCg         >  2:1612120/1‑140 (MQ=255)
                                                                                                                                      ttCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTggg    >  2:1724858/1‑140 (MQ=255)
                                                                                                                                        cTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGc  <  1:1324117/140‑1 (MQ=255)
                                                                                                                                        cTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGc  <  1:1170757/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGAGACGCTGCCTTCAGTAACCAGCGCCTGTTAATCGCCAATTTCTTTGTTGCAGAAAAAGTTCTGCAAGATCTTGTTCTGCAACTCCACCCACGTTCAACCTGGCATTCTTTTTTGCCAGCAAAACGTATGGATATTGTTGTGAGCGCACTGGAAATGAATGAGGGCGGTTTGTCACAGGTTGAGGAACGCATTCTCCATGAAGTGGTCGCTGGGGC  >  NC_000913/4213251‑4213526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑