Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,304,455 C→A 6.4% intergenic (‑52/+157) ytcA ← / ← yjcS putative inner membrane efflux pump‑associated DUF1656 family protein/metallo‑beta‑lactamase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,304,4550CA 6.4% 220.2 / 2.7 63intergenic (‑52/+157)ytcA/yjcSputative inner membrane efflux pump‑associated DUF1656 family protein/metallo‑beta‑lactamase superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (33/26);  new base A (2/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

AGCATCCCGAAACCATGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAGCGC  >  NC_000913/4304322‑4304593
                                                                                                                                     |                                                                                                                                          
aGCATCCCGAAACCATGTTGACGGGAATTATCCATGCTGTTTTTTTGAGTTGCATCGACATGCGAGAGAGAACTGTTGGCATATTAAATAATTCCTGTGTTCTTCGAACAATATCTGTAGTGAATATAAATTTCCATTGa                                                                                                                                      <  2:1610260/140‑1 (MQ=255)
aGCATCCCGAAACCATGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATTTCCATTGa                                                                                                                                      <  2:141159/140‑1 (MQ=255)
 gCATCCCGAAACCATGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTgga                                                                                                                                     <  1:1577212/140‑3 (MQ=255)
  cATCCCGAAACCATGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAg                                                                                                                                    >  1:1093285/1‑140 (MQ=255)
    tCCCGAAACCATGAAGACGGTAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATACATTGAAGGc                                                                                                                                  >  1:1617844/1‑140 (MQ=255)
    tCCCGAAACCATGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGc                                                                                                                                  >  1:1587318/1‑140 (MQ=255)
    tCCCGAAACCATGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATACATTGAAGGc                                                                                                                                  >  1:1616236/1‑140 (MQ=255)
    tCCCGAAACCAAGAAGACCGGAATTATCCAGGAAGTATTATTGAGTTGCATCGCCATGAGAGAGAGAAATTATGGCATATTAAATACATCCTGTCTGCAGCGAACAATATCTGTAGTGAATATCAATATCCATAGAAGGc                                                                                                                                  <  2:1877414/140‑1 (MQ=255)
              aTGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATgg                                                                                                                        <  1:1147660/140‑1 (MQ=255)
              aTGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATgg                                                                                                                        <  1:1639854/140‑1 (MQ=255)
                        gAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCtt                                                                                                              >  2:8393/1‑140 (MQ=255)
                               ccAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAAt                                                                                                       <  2:987501/140‑1 (MQ=255)
                               ccAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAAt                                                                                                       <  2:476184/140‑1 (MQ=255)
                                  ggCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGTTGAATaaa                                                                                                    >  1:1535386/1‑140 (MQ=255)
                                   gccgTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaa                                                                                                   >  1:975096/4‑140 (MQ=255)
                                   gCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaa                                                                                                   >  2:1523007/1‑140 (MQ=255)
                                   gCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaa                                                                                                   >  2:954479/1‑140 (MQ=255)
                                   gCTGTTTTTTAGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaa                                                                                                   >  2:902180/1‑140 (MQ=255)
                                    cTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGCACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGAGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaaa                                                                                                  <  2:38025/140‑1 (MQ=255)
                                    cTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaaa                                                                                                  <  1:961785/140‑1 (MQ=255)
                                    cTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaaa                                                                                                  <  2:1745247/140‑1 (MQ=255)
                                    cTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaaa                                                                                                  <  1:1067899/140‑1 (MQ=255)
                                    cTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATaaaaa                                                                                                  <  1:515104/140‑1 (MQ=255)
                                                 ttGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAg                                                                                     >  2:422663/1‑140 (MQ=255)
                                                      tCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCAcc                                                                                <  2:1405057/140‑1 (MQ=255)
                                                      tCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCAcc                                                                                <  1:850159/140‑1 (MQ=255)
                                                              cGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCAt                                                                        >  1:1073921/1‑140 (MQ=255)
                                                              cGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCAt                                                                        >  2:125357/1‑140 (MQ=255)
                                                              cGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCAt                                                                        <  1:338912/140‑1 (MQ=255)
                                                              cGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCAt                                                                        <  1:338876/140‑1 (MQ=255)
                                                              cGAGAGAGAACTGTTGGCATAATAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAAGATCAATATCCATTGAAGGCGGCAGTATGGCTAGTTTCTGGATGAATAAAAATAGCTGTATTCAGCCACCGCAGTCag                                                                        >  1:565636/1‑139 (MQ=255)
                                                                           ttGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATAtattt                                                           >  1:1304233/1‑140 (MQ=255)
                                                                             ggCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATAtatttta                                                         >  1:689304/1‑140 (MQ=255)
                                                                               cATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATAtattttatt                                                       <  2:28745/140‑1 (MQ=255)
                                                                                atatTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCAcc                                                                                <  1:723057/114‑1 (MQ=255)
                                                                                atatTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCAcc                                                                                >  2:723057/1‑114 (MQ=255)
                                                                                atatTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATAtattttattt                                                      >  2:732981/1‑140 (MQ=255)
                                                                                atatTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGAATGGCTATTTTCATGATGAATAAATAGAGCTTTATTCAGCCACCGCATTCATAACTTCTAtattttattt                                                      >  1:614330/1‑140 (MQ=255)
                                                                                   ttAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTtatttatt                                                   >  1:1785887/1‑140 (MQ=255)
                                                                                   ttAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTtatttatt                                                   >  1:1785697/1‑140 (MQ=255)
                                                                                   ttAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTtatttatt                                                   >  2:1437052/1‑140 (MQ=255)
                                                                                      aaTACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATACATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAAt                                                <  2:1616236/140‑1 (MQ=255)
                                                                                      aaTACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATACATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAAt                                                <  2:1617844/140‑1 (MQ=255)
                                                                                         aCATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTgg                                             <  1:1784265/140‑1 (MQ=255)
                                                                                             ccTGTCTGCTGCGAACAATATCTGTGGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGt                                         >  1:298394/1‑140 (MQ=255)
                                                                                             ccTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGt                                         >  1:298013/1‑140 (MQ=255)
                                                                                             ccTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGt                                         >  1:1339201/1‑140 (MQ=255)
                                                                                                gTCTGCTGCGAACAATATATGTTGTTAATATCAATATCCATTGTAGGCGTCAGTATGTCTATTTTCGTGATGAATAAAAATAGCTTTATTCAGCCTCAGCATTCATAACTTTTATATTTTTTTTATTATTTGGCTGtata                                      <  2:541026/140‑1 (MQ=255)
                                                                                                 tCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGtatat                                     >  1:1096920/1‑140 (MQ=255)
                                                                                                       gcGAACACTATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATAttttt                               <  2:1508083/140‑1 (MQ=255)
                                                                                                       gcGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATAttttt                               >  1:1606942/1‑140 (MQ=255)
                                                                                                       gcGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATAttttt                               <  1:1875719/140‑1 (MQ=255)
                                                                                                        cGAACAATATCTCTAGAGAATATCAATACCCATTGAAGGCGTCAGTATGGCTAATTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTa                              <  1:440209/140‑1 (MQ=255)
                                                                                                          aaCAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTaaa                            >  2:1589601/1‑140 (MQ=255)
                                                                                                          aaCAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTaaa                            >  2:1192051/1‑140 (MQ=255)
                                                                                                          aaCAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTaaa                            >  2:1192075/1‑140 (MQ=255)
                                                                                                               tatCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTCTATATTTTATTTATTAATTGGCTGTATATATTTTTAaaataaa                       <  1:532356/140‑1 (MQ=255)
                                                                                                                  cTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATAc                    >  1:826063/1‑140 (MQ=255)
                                                                                                                       gTGAATATCAATATCCATTGAAGGCGTCTGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTg               >  2:1750640/1‑140 (MQ=255)
                                                                                                                       gTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTgg                                             >  2:1467304/1‑110 (MQ=255)
                                                                                                                       gTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTgg                                             <  1:1467304/110‑1 (MQ=255)
                                                                                                                       gTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTg               >  2:1750705/1‑140 (MQ=255)
                                                                                                                       gTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTg               >  2:1421834/1‑140 (MQ=255)
                                                                                                                          aatatcaatatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCATCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTgggg            >  1:1404659/1‑140 (MQ=255)
                                                                                                                          aatatcaatatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTgggg            >  1:273733/1‑140 (MQ=255)
                                                                                                                          aatatcaatatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTgggg            >  2:1674209/1‑140 (MQ=255)
                                                                                                                          aatatcaatatcCATTGAAGGCGTCAGTATGGCTATTATCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATAAATTGGCTGTATATATTTTTAAAATAAATACTTATgggg            >  1:1577808/1‑140 (MQ=255)
                                                                                                                            taacaatatcCATTGAAGGCGTCAGTATGGATATTTACTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGc          <  1:1909317/137‑1 (MQ=255)
                                                                                                                             atcaatatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCt         >  2:1065118/1‑140 (MQ=255)
                                                                                                                               caatatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTc       >  2:194427/1‑140 (MQ=255)
                                                                                                                               caatatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTc       >  1:1420471/1‑140 (MQ=255)
                                                                                                                                  tatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAgc    <  2:21369/140‑1 (MQ=255)
                                                                                                                                  tatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAgc    <  2:21299/140‑1 (MQ=255)
                                                                                                                                  tatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAgc    >  1:1359218/1‑140 (MQ=255)
                                                                                                                                  tatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAgc    <  2:1529569/140‑1 (MQ=255)
                                                                                                                                  gatcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAgc    <  2:21239/139‑1 (MQ=255)
                                                                                                                                    tcCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAgcgc  >  2:507080/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                          
AGCATCCCGAAACCATGAAGACGGGAATTATCCAGGCTGTTTTTTTGAGTTGCATCGCCATGCGAGAGAGAACTGTTGGCATATTAAATACATCCTGTCTGCTGCGAACAATATCTGTAGTGAATATCAATATCCATTGAAGGCGTCAGTATGGCTATTTTCTTGATGAATAAAAATAGCTTTATTCAGCCACCGCATTCATAACTTATATATTTTATTTATTAATTGGCTGTATATATTTTTAAAATAAATACTTTTGGGGGCTTCAGCGC  >  NC_000913/4304322‑4304593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: